FastQCFastQC Report
Tue 31 May 2016
SRR212665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46266
Sequences flagged as poor quality0
Sequence length58
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT22384.837245493450915No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC16863.6441447283102066No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7301.5778325336099943No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6801.4697618121298577No Hit
CCGCTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5551.1995850084295163No Hit
CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG5521.1931007651407082No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4300.9294082047291747No Hit
CCGCTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4200.9077940604331475No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3790.8191760688194355No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT3510.7586564647905589No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2350.5079323909566421No Hit
CCGCTAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA2340.5057709765270393No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT2290.49496390437902565No Hit
CCGCTAGGGCCTTTTCCTGTGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2180.47118834565339557No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA2120.45821985907577917No Hit
CCGCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1950.42147581377253274No Hit
CCGCTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1860.40202308390610814No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1850.3998616694765055No Hit
CCGCTAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1520.3285349932996152No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1390.30043660571477976No Hit
CCGCTAGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTT1380.29827519128517704No Hit
CCGCTAGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGG1370.2961137768555743No Hit
CCGCTAGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCGCC1320.28530670470756064No Hit
CCGCTAGGGGGGGCTGGAGAGATGGTTCAATGGATAAGAGCACTGACTGCTCTTCCAA1320.28530670470756064No Hit
CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1310.2831452902779579No Hit
CCGCTAGGGGCCACAGCCATCTCCAGGACTGAATTATGTTCCAAAAAAAAGCATCCCC1240.26801538927073876No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA1190.2572083171227251No Hit
CCGCTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1180.25504690269312236No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA1160.2507240738339169No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1150.24856265940431418No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCC1140.24640124497471144No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1100.23775558725630053No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGAAC1070.23127134396749233No Hit
CCGCTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1000.21614144296027318No Hit
CCGCTAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1000.21614144296027318No Hit
CCGCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA980.21181861410106773No Hit
CCGCTAGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCCCCCCATATCGG970.209657199671465No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA960.20749578524186227No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA950.20533437081225953No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC900.19452729866424587No Hit
CCGCTAGGGGGGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTTTGCCCGCCGTGAT860.18588164094583495No Hit
CCGCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA850.1837202265162322No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC840.1815588120866295No Hit
CCGCTAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC830.17939739765702675No Hit
CCGCTAGGGGCTCCGCCGTCTGCCACTGCCGCCTGGGCTCACTGAGTGGTTCATCTGG820.177235983227424No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA790.1707517399386158No Hit
CCGCTAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA790.1707517399386158No Hit
CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT780.1685903255090131No Hit
CCGCTAGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT740.15994466779060218No Hit
CCGCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT720.1556218389313967No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA710.15346042450179398No Hit
CCGCTAGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA710.15346042450179398No Hit
CCGCTAGGGGAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGATTGCTCTTCCAG700.15129901007219124No Hit
CCGCTAGGGTAGAAGGGGGTTTTCTCAACCTCTGGCTGCTGAGAGCGATACTTCAGAA700.15129901007219124No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC690.14913759564258852No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.14697618121298578No Hit
CCGCTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA670.14481476678338304No Hit
CCGCTAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC670.14481476678338304No Hit
CCGCTAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA660.14265335235378032No Hit
CCGCTAGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA650.14049193792417758No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGGCC630.13616910906497212No Hit
CCGCTAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA620.13400769463536938No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA620.13400769463536938No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC620.13400769463536938No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC620.13400769463536938No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA610.13184628020576664No Hit
CCGCTAGGGGCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCACG590.12752345134656118No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCGA570.12320062248735572No Hit
CCGCTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT570.12320062248735572No Hit
CCGCTAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG570.12320062248735572No Hit
CCGCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA560.12103920805775299No Hit
CCGCTAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG550.11887779362815026No Hit
CCGCTAGGGGAGCTGGCTGGTCGGGGACAGGAAAGAAGGAAGAACTAACTCAGATAAA550.11887779362815026No Hit
CCGCTAGGGAGCCAAAGCAACACCTCGCACTCCTGCCCCAGCATGCAGAAGGACGCCT540.11671637919854753No Hit
CCGCTAGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGACA540.11671637919854753No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT540.11671637919854753No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA530.11455496476894479No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTCTTCTGA520.11239355033934206No Hit
CCGCTAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG520.11239355033934206No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGAA520.11239355033934206No Hit
CCGCTAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCA510.11023213590973933No Hit
CCGCTAGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC510.11023213590973933No Hit
CCGCTAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT500.10807072148013659No Hit
CCGCTAGGGGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA500.10807072148013659No Hit
CCGCTAGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT490.10590930705053386No Hit
CCGCTAGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC490.10590930705053386No Hit
CCGCTAGGGGAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTG490.10590930705053386No Hit
CCGCTAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT480.10374789262093113No Hit
CCGCTAGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTT480.10374789262093113No Hit
CCGCTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC480.10374789262093113No Hit
CCGCTAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA480.10374789262093113No Hit
CCGCTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA480.10374789262093113No Hit
CCGCTAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGC470.1015864781913284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGG150.006970571452.383619
ATGGGGG150.006970571452.3836110
GCCTCTT150.006970571452.3836110
AGGGCTT1100.052.383616
AGGGTCC150.006970571452.383616
GTCCTTG150.006970571452.3836110
GGTGGGC150.006970571452.3836110
GTGGTCA150.006970571452.383619
TAGGGTG307.43923E-752.383615
TAGGGGC15450.052.383615
TAGGGCT1650.052.383615
GGGTGCT150.006970571452.383618
GGAGTTG150.006970571452.383619
AGGGGGA1800.052.3836066
AGGGAGT251.5607422E-552.3836066
AGGGACT353.561945E-852.3836066
GGAAGCA503.6379788E-1252.3836068
GGAGGGC251.5607422E-552.3836068
TAGGGCC1350.052.3836065
TAGGGAC650.052.3836065
Produced by FastQC (version 0.11.5)