FastQCFastQC Report
Tue 31 May 2016
SRR212664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55758
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC31985.735499838588185No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16632.9825316546504537No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC8401.5065102765522436No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA7001.255425230460203No Hit
CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA6591.181893181247534No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6471.160371605868216No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC5791.0384160120520822No Hit
CCGCTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5701.022274830517594No Hit
CCGCTAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC4280.7676028551956671No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC4160.7460812798163493No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4020.7209727752071452No Hit
CCGCTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3720.6671688367588507No Hit
CCGCTAGGGGGGGCTGGAGAGATGGTTCAATGGATAAGAGCACTGACTGCTCTTCC3590.6438537967645899No Hit
CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3460.620538756770329No Hit
CCGCTAGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT3460.620538756770329No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2900.5201047383335127No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2690.48244198141970657No Hit
CCGCTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC2680.4806485168047634No Hit
CCGCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2080.37304063990817465No Hit
CCGCTAGGGCCTTTTCCTGTGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1900.34075827683919796No Hit
CCGCTAGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTC1720.3084759137702213No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC1600.28695433839090356No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.2744000860863015No Hit
CCGCTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1510.2708131568564152No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGA1480.26543276301158575No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1460.2618458337816995No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA1440.2582589045518132No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC1440.2582589045518132No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1410.25287851070698375No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCA1340.2403242584023817No Hit
CCGCTAGGGGCCACAGCCATCTCCAGGACTGAATTATGTTCCAAAAAAAAGCATCC1300.23315039994260914No Hit
CCGCTAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1300.23315039994260914No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT1260.22597654148283655No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCC1230.2205961476380071No Hit
CCGCTAGGGGGCCGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGG1230.2205961476380071No Hit
CCGCTAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT1230.2205961476380071No Hit
CCGCTAGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1220.21880268302306394No Hit
CCGCTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1210.2170092184081208No Hit
CCGCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1210.2170092184081208No Hit
CCGCTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1200.21521575379317764No Hit
CCGCTAGGGGGGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTTTGCCCGCCGTG1160.20804189533340506No Hit
CCGCTAGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCG1140.20445496610351876No Hit
CCGCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1130.20266150148857562No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTCTTCT1130.20266150148857562No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACA1070.19190071379891677No Hit
CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1070.19190071379891677No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG990.17755299687937157No Hit
CCGCTAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGAT940.16858567380465583No Hit
CCGCTAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC920.16499874457476954No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC900.16141181534488325No Hit
CCGCTAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG860.15423795688511066No Hit
CCGCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCC850.15244449227016751No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA840.15065102765522434No Hit
CCGCTAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC840.15065102765522434No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTC840.15065102765522434No Hit
CCGCTAGGGTAGAAGGGGGTTTTCTCAACCTCTGGCTGCTGAGAGCGATACTTCAG820.14706409842533807No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC820.14706409842533807No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA810.1452706338103949No Hit
CCGCTAGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCC800.14347716919545178No Hit
CCGCTAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA780.1398902399655655No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT770.13809677535062231No Hit
CCGCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC770.13809677535062231No Hit
CCGCTAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC760.1363033107356792No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTAAGTAAGAGCACCCGACTGCTCTT760.1363033107356792No Hit
CCGCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC760.1363033107356792No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC740.13271638150579287No Hit
CCGCTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT700.12554252304602032No Hit
CCGCTAGGGGAGCTGGCTGGTCGGGGACAGGAAAGAAGGAAGAACTAACTCAGATA690.12374905843107716No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGTTCAATGGATAAGAGCACTGACTGCTCTTC670.12016212920119085No Hit
CCGCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG670.12016212920119085No Hit
CCGCTAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG650.11657519997130457No Hit
CCGCTAGGGGAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGATTGCTCTTCC640.11478173535636141No Hit
CCGCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA640.11478173535636141No Hit
CCGCTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA630.11298827074141828No Hit
CCGCTAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT630.11298827074141828No Hit
CCGCTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC630.11298827074141828No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCCCC620.11119480612647513No Hit
CCGCTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG610.10940134151153197No Hit
CCGCTAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA600.10760787689658882No Hit
CCGCTAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC600.10760787689658882No Hit
CCGCTAGGGAGTCTTAGCCCCAGGGCAAATTCTTGCTGCAGGACAGATAGTTTGGT600.10760787689658882No Hit
CCGCTAGGGGGGGGCTGGCGAGATGGCTCAGAGGTTAAGAGCGCCGACTGCTCTTC590.10581441228164569No Hit
CCGCTAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG580.10402094766670253No Hit
CCGCTAGGGGCTCCGCCGTCTGCCACTGCCGCCTGGGCTCACTGAGTGGTTCATCT580.10402094766670253No Hit
CCGCTAGGGGAGATTCTAAGGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAGA570.10222748305175938No Hit
CCGCTAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC560.10043401843681625No Hit
CCGCTAGGGGCTCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCC560.10043401843681625No Hit
CCGCTAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA560.10043401843681625No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC560.10043401843681625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATG251.991538E-550.2929550
AACGCAC150.00819741850.29294649
ACTGAGA150.00819741850.29294650
CCAGCAG150.00819741850.29294650
ACGCACC150.00819741850.29294650
AGAGGAC150.00819741850.29294650
TGATACA451.2369128E-1050.29294650
GATCTCA150.00819741850.29294649
ATGCAGA150.00819741850.29294649
TAGAGGA150.00819741850.29294649
CGCCGTG150.00819741850.29294650
CTTCCAA150.00819741850.29294650
TCCCCTA150.00819741850.29294649
CAAGAAA204.0493303E-450.24751748
GGATCTC150.00822679150.24751748
GATGCAG150.00822679150.24751748
GATCTTC150.00822679150.24751748
TTTGGTA204.0493303E-450.24751748
CGACTGA150.00822679150.24751748
TAAACGC150.00825624450.20216847
Produced by FastQC (version 0.11.5)