##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212664_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55758 Sequences flagged as poor quality 0 Sequence length 56 %GC 59 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.223250475268124 39.0 38.0 40.0 35.0 40.0 2 38.1861616270311 39.0 38.0 40.0 35.0 40.0 3 37.46022095484056 39.0 37.0 40.0 33.0 40.0 4 37.3833171921518 39.0 38.0 40.0 33.0 40.0 5 37.551508303741166 39.0 38.0 40.0 33.0 40.0 6 38.05821586140105 39.0 38.0 40.0 35.0 40.0 7 38.43184834463216 39.0 38.0 40.0 36.0 40.0 8 38.41798486315865 39.0 38.0 40.0 36.0 40.0 9 38.37673517701496 39.0 38.0 40.0 36.0 40.0 10 37.75729043365974 39.0 38.0 40.0 33.0 40.0 11 37.59682915456078 39.0 38.0 40.0 33.0 40.0 12 37.40512572186951 39.0 37.0 40.0 33.0 40.0 13 37.33821155708598 39.0 37.0 40.0 32.0 40.0 14 37.43773090856917 39.0 37.0 40.0 33.0 40.0 15 37.6325370350443 39.0 38.0 40.0 33.0 40.0 16 37.49759675741598 39.0 38.0 40.0 33.0 40.0 17 37.056673481832206 39.0 36.0 40.0 33.0 40.0 18 37.02778076688547 39.0 36.0 40.0 33.0 40.0 19 36.86231572151082 38.0 36.0 40.0 32.0 40.0 20 36.68287958678575 38.0 36.0 40.0 31.0 40.0 21 36.366081997202194 38.0 35.0 40.0 31.0 40.0 22 36.375300405323 38.0 35.0 40.0 31.0 40.0 23 36.22836184942071 38.0 35.0 40.0 30.0 40.0 24 35.90091108002439 38.0 35.0 39.0 30.0 40.0 25 35.77151260805624 38.0 35.0 39.0 30.0 40.0 26 35.20391692671904 38.0 35.0 39.0 29.0 40.0 27 34.88146992359841 38.0 34.0 39.0 28.0 40.0 28 34.458786183148604 37.0 33.0 39.0 27.0 40.0 29 34.37289716273898 37.0 33.0 39.0 27.0 40.0 30 34.24093403637146 38.0 33.0 39.0 26.0 40.0 31 33.10574267369705 36.0 32.0 38.0 23.0 40.0 32 32.976218659205855 36.0 32.0 38.0 23.0 40.0 33 32.824545356720115 36.0 32.0 38.0 22.0 40.0 34 32.14109186125758 35.0 31.0 38.0 19.0 40.0 35 31.645934215717922 35.0 30.0 38.0 17.0 40.0 36 32.083019477025715 36.0 32.0 38.0 17.0 40.0 37 31.435076580939057 35.0 30.0 38.0 10.0 40.0 38 30.772014778148428 35.0 30.0 38.0 2.0 40.0 39 30.843430539115463 35.0 30.0 38.0 2.0 40.0 40 30.44038523619929 35.0 29.0 38.0 2.0 40.0 41 30.106173105204633 35.0 29.0 38.0 2.0 40.0 42 29.528031851931562 35.0 27.0 38.0 2.0 40.0 43 29.012518383012303 34.0 27.0 38.0 2.0 40.0 44 29.289662469959467 35.0 28.0 38.0 2.0 40.0 45 28.66114279565264 34.0 27.0 38.0 2.0 39.0 46 27.491983213171203 33.0 23.0 38.0 2.0 39.0 47 26.92494350586463 33.0 21.0 37.0 2.0 39.0 48 27.523315039994262 33.0 25.0 37.0 2.0 39.0 49 27.04166218300513 33.0 23.0 37.0 2.0 39.0 50 26.691757236629723 33.0 21.0 37.0 2.0 39.0 51 25.946554754474693 33.0 2.0 37.0 2.0 39.0 52 25.51063524516661 33.0 2.0 36.0 2.0 39.0 53 25.176476918110406 32.0 2.0 36.0 2.0 39.0 54 24.90001434771692 32.0 2.0 36.0 2.0 39.0 55 24.444922701675097 32.0 2.0 36.0 2.0 39.0 56 14.343143584777073 18.0 2.0 23.0 2.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.37585552193888816 1 2 -0.6163851293105154 1 3 -0.8862177270646754 1 4 -0.7720018293642497 1 5 -1.5546744328885538 1 6 -0.9726027300208457 1 7 -0.8344937542276867 1 8 -0.6627302629165754 1 9 -0.620370826824427 1 10 -1.7424470009940478 1 11 -1.6030383868066949 1 12 -2.642272486593228 1 13 -1.4681332351197156 1 14 -2.5631369649901643 1 15 -2.4586702191692424 1 16 -2.999577555754769 1 17 -2.1664862685042294 1 18 -1.8907384534238716 1 19 -3.0401252372368717 1 20 -1.4940937908058558 1 21 -1.7624455901180909 1 22 -1.5389338951086273 1 23 -3.5109776278130838 1 24 -1.0245815527094493 1 25 -1.6261698761781815 1 26 -1.455023994742021 1 27 -0.7339426588393252 1 28 -1.8004778542166449 1 29 -0.4168214631254301 1 30 -2.3784395272252326 1 31 1.0555834504060044 1 32 -1.0314024018365018 1 33 1.1481868030344913 1 34 -0.43392408592334064 1 35 0.5215198116473374 1 36 -0.5188390078183893 1 37 -0.4755113450776278 1 38 0.9759702441980913 1 39 1.2549911924368402 1 40 -1.7486084265122486 1 41 1.085852680190758 1 42 0.34149656464192546 1 43 2.9266130287308023 1 44 2.338358124884568 1 45 2.40202412945899 1 46 3.286697779626266 1 47 4.166122762307651 1 48 3.7469433234543956 1 49 3.943510146117543 1 50 3.0786436108822173 1 51 3.785051320013409 1 52 2.602432579814252 1 53 2.7949129481865995 1 54 3.3400358940672774 1 55 4.615588955963684 1 56 2.7127217436004027 2 1 -0.8850098375448141 2 2 -0.137745199057683 2 3 -1.2349535596714745 2 4 -0.7484621606632871 2 5 -0.582573299497092 2 6 0.07098924905500326 2 7 -0.009733510112603483 2 8 0.21695587483407763 2 9 0.07012612173704014 2 10 0.14944489089784696 2 11 0.18074539697708758 2 12 -0.762586348842575 2 13 -0.6041402098363662 2 14 0.2947531832225607 2 15 0.3695773832719098 2 16 0.27330823326005316 2 17 0.4167744115306391 2 18 -0.18716391113965614 2 19 1.0671110312897198 2 20 0.4745199842595298 2 21 -0.08938543318696901 2 22 -0.10301410435013025 2 23 0.02258819607531848 2 24 0.39041408809264055 2 25 1.3145450322786658 2 26 -0.42450960938892734 2 27 1.7621340630438524 2 28 1.0940295564197982 2 29 0.9877121026984597 2 30 -0.9904709134501672 2 31 1.9649121339282374 2 32 2.027882689706651 2 33 1.2554230715610828 2 34 1.6724403430217336 2 35 1.9330280941233582 2 36 2.948467007874722 2 37 1.8863020812519302 2 38 2.244496835305327 2 39 3.092829030274679 2 40 3.844242488919317 2 41 2.6595231248289437 2 42 0.03286535278490632 2 43 2.271861503011536 2 44 1.585088726453879 2 45 1.5755202061808724 2 46 3.4697840917448346 2 47 4.420699920110614 2 48 2.9701342563227477 2 49 2.3166574870068217 2 50 1.1475189378220598 2 51 1.3787740750264845 2 52 2.2249260410173903 2 53 2.6711117276111835 2 54 0.9686322323410366 2 55 2.373217552301959 2 56 0.5139423190145251 3 1 -1.1823071348421124 3 2 -1.067211250474955 3 3 -0.7847228411480742 3 4 -1.1756210268465423 3 5 -1.1494816712835174 3 6 -0.6956554444189536 3 7 -0.9029437935667843 3 8 -1.0388120882509568 3 9 -0.9476721643539321 3 10 -1.1326909034330725 3 11 -1.2371847282701083 3 12 -1.7012811411959063 3 13 -0.892208766197605 3 14 -1.6386680428815907 3 15 -1.7332571585870227 3 16 -0.6785704747162171 3 17 -0.484346221297308 3 18 -1.7105653613703709 3 19 -2.2202983292903724 3 20 -2.175447055951409 3 21 -2.3517453540834694 3 22 -1.6962903073824336 3 23 -1.0255330959484112 3 24 -1.8475555889014217 3 25 -2.3657450138650447 3 26 -2.2221365045768025 3 27 -0.5081362072264213 3 28 -2.162397602446383 3 29 -1.4262628478618566 3 30 -1.3965355146367173 3 31 -1.7555888548654366 3 32 -2.3649979958569602 3 33 -3.617352142677543 3 34 -1.4284166114937555 3 35 -0.6806831781245002 3 36 -1.8241104476295646 3 37 -2.850019606289269 3 38 -3.1160832569820798 3 39 -3.2084234417095026 3 40 -2.7674911959851016 3 41 -2.3114722608005884 3 42 -1.9031926564438315 3 43 -1.8955742253998054 3 44 -3.3311934093404503 3 45 -3.206945186040617 3 46 -0.6086602062116562 3 47 -1.3940660653870793 3 48 -1.2263060864590578 3 49 -0.9424064549839528 3 50 0.06841544408441891 3 51 -0.7853656744791166 3 52 -0.16861384032736737 3 53 -0.05795880633739969 3 54 0.366786484153824 3 55 0.06405473094401515 3 56 -1.3423530006954287 4 1 0.09269286515788622 4 2 0.11022777391529104 4 3 0.004911305193388671 4 4 -0.12684053904166603 4 5 0.15661588969209106 4 6 -0.28711885905310197 4 7 0.3598610844819916 4 8 0.2203342532124566 4 9 0.36269368930460644 4 10 -0.29244700099404497 4 11 -0.7030383868066963 4 12 0.19079202953580676 4 13 -0.10684291253906508 4 14 -0.022814384345004157 4 15 -0.6594766707821478 4 16 -0.38344852349670333 4 17 -0.044712074955846504 4 18 -0.32702877600451785 4 19 0.371165085343776 4 20 -0.8626421779026288 4 21 0.9964253776238436 4 22 0.44090481456878905 4 23 -0.2206550471679236 4 24 0.27622489890345747 4 25 0.8665720593056889 4 26 1.3498147149353983 4 27 1.1418637927735773 4 28 0.7406511780414178 4 29 2.3694688594552176 4 30 1.8473669243876714 4 31 1.104776998793099 4 32 1.6258556626796263 4 33 0.4094771256151404 4 34 1.8886565592379512 4 35 -0.38251244641718074 4 36 0.3795480889558043 4 37 1.0640047839546334 4 38 2.248550889359386 4 39 2.1799911924368374 4 40 2.1239722186490404 4 41 2.2318204221262405 4 42 3.588270758190312 4 43 2.730645286795319 4 44 2.443196834561988 4 45 2.0302499359106037 4 46 -0.2342699623092166 4 47 -0.7056514312407351 4 48 -1.0433792571907645 4 49 -1.138747918398586 4 50 -0.2882918729887507 4 51 -0.25365835740594633 4 52 -0.4588577427663907 4 53 1.1231387546382123 4 54 -0.050286686577884154 4 55 0.3042986333830413 4 56 -0.18808470801250188 5 1 0.15102619849122334 5 2 0.24548418417170126 5 3 0.5017061769882574 5 4 0.8475184353173049 5 5 0.9143081973844005 5 6 0.6039067819725403 5 7 0.5592200588409639 5 8 0.3389239968022011 5 9 0.4069244585353715 5 10 0.4113991528521055 5 11 0.5764487926804875 5 12 0.8722022859460594 5 13 1.205977600281443 5 14 0.8553907438601271 5 15 0.7514207651152915 5 16 0.5421925021443244 5 17 0.3149033096595417 5 18 0.5633558393800939 5 19 0.4288573930360826 5 20 1.132870642610186 5 21 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1.4329849323956267 6 48 1.3657116519001455 6 49 1.8885248088741413 6 50 1.632162672465796 6 51 2.4917961880485997 6 52 1.8729604390517913 6 53 2.561775118274575 6 54 1.0497133134221173 6 55 0.9997531788375866 6 56 1.7823698374420456 7 1 -0.8323071348421109 7 2 -0.7897722260847075 7 3 -0.6200886948066113 7 4 -1.2768405390416646 7 5 -1.3683841103079075 7 6 -0.8121188590531006 7 7 -0.665138915518007 7 8 -0.7546657467875448 7 9 -0.9373063106953978 7 10 -1.1174470009940478 7 11 -0.5530383868066977 7 12 -0.5092079704641961 7 13 -1.2568429125390708 7 14 -0.3228143843450013 7 15 -0.05947667078214636 7 16 -0.23344852349670475 7 17 -1.0447120749558465 7 18 -1.1020287760045164 7 19 -1.4788349146562254 7 20 -1.6376421779026344 7 21 -1.528574622376155 7 22 -1.734095185431208 7 23 -1.7206550471679236 7 24 -1.6487751010965468 7 25 -2.383427940694311 7 26 0.37481471493539686 7 27 0.6668637927735759 7 28 0.29065117804142204 7 29 0.5444688594552147 7 30 -1.7026330756123258 7 31 -1.6202230012068988 7 32 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3.5568204221262434 118 42 1.963270758190312 118 43 3.255645286795321 118 44 4.068196834561988 118 45 2.3802499359106015 118 46 2.0907300376907827 118 47 1.0193485687592627 118 48 1.3816207428092362 118 49 0.31125208160141327 118 50 0.5867081270112493 118 51 0.49634164259405367 118 52 0.8161422572336079 118 53 1.1981387546382116 118 54 0.17471331342211727 118 55 -1.1457013666169615 118 56 -1.4630847080125005 119 1 -0.7323071348421095 119 2 -0.4647722260847047 119 3 -0.9450886948066142 119 4 -0.20184053904166888 119 5 -0.7433841103079075 119 6 -0.8371188590530991 119 7 -0.3401389155180041 119 8 -0.32966574678754057 119 9 -1.0373063106953921 119 10 -1.417447000994045 119 11 -1.5780383868066963 119 12 -1.009207970464196 119 13 -1.731842912539065 119 14 -1.3978143843450042 119 15 -1.334476670782145 119 16 -1.1834485234967076 119 17 -3.0447120749558465 119 18 -2.7270287760045164 119 19 -1.8538349146562254 119 20 -1.0876421779026302 119 21 -1.0535746223761535 119 22 -1.7840951854312124 119 23 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-0.8117292418096866 120 43 0.8368952867953183 120 44 1.0994468345619879 120 45 0.7114999359106022 120 46 1.978230037690782 120 47 1.4755985687592634 120 48 1.1441207428092355 120 49 1.3487520816014147 120 50 1.6179581270112493 120 51 1.571341642594053 120 52 2.097392257233608 120 53 2.3231387546382116 120 54 1.4247133134221173 120 55 0.23554863338303988 120 56 1.5181652919874988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20.0 3 2.0 4 5.0 5 10.0 6 15.0 7 34.0 8 34.0 9 50.0 10 77.0 11 86.0 12 157.0 13 172.0 14 250.0 15 256.0 16 343.0 17 413.0 18 517.0 19 578.0 20 603.0 21 617.0 22 684.0 23 860.0 24 1078.0 25 1319.0 26 1253.0 27 1150.0 28 1197.0 29 1461.0 30 1652.0 31 1871.0 32 2477.0 33 3134.0 34 4084.0 35 5503.0 36 7058.0 37 7847.0 38 6536.0 39 2355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 78.80662864521682 11.08899171419348 2.288460848667456 7.815918791922235 11 39.37910255030668 14.432009756447506 9.16281071774454 37.02607697550127 12 37.33275942465655 9.100039456221529 29.769719143441296 23.797481975680622 13 21.93407224075469 14.43380322106245 36.78575271709889 26.84637182108397 14 14.896517091717781 15.866781448402023 47.91420065282112 21.32250080705908 15 20.371964561139208 11.747193227877615 49.38125470784461 18.499587503138564 16 26.137953298181426 11.503282040245345 36.396570895656225 25.962193765916997 17 26.143333692026253 10.775135406578428 20.074249435058647 43.007281466336664 18 29.674665518849313 12.071810323182323 25.983715341296314 32.26980881667205 19 32.110190465942104 15.850640266867536 29.30879873740091 22.730370529789447 20 35.90695505577675 16.342049571361958 27.08310915025647 20.667886222604828 21 32.054593062878865 15.135047885505218 31.342587610746442 21.46777144086947 22 39.96556547939309 15.592381362315722 25.232253667635135 19.20979949065605 23 45.18813443810753 16.365364611356217 22.73754438824922 15.708956562287026 24 39.18540837189282 20.574626062627786 17.841385989454427 22.398579576024964 25 30.655231807090573 26.740347351801347 15.828190038754125 26.776230802353957 26 31.746231155778894 15.673007896625988 21.81442928930366 30.766331658291456 27 21.88111524030089 18.728568607385863 22.41072871223138 36.979587440081865 28 28.458803246893183 27.548308311184545 18.43797141009985 25.554917031822427 29 32.94170484146232 16.448396658582592 22.11084164196533 28.499056857989757 30 22.74418186719382 16.905382334441548 24.28250516668164 36.06793063168299 31 33.11284466333537 14.624150699212713 25.570694179818098 26.692310457633823 32 46.910849786025096 12.19117488402201 23.45632394720754 17.441651382745352 33 50.355298900822135 9.860219115980355 21.249572741828125 18.534909241369384 34 33.377714604436925 15.95206822720812 33.962467838572124 16.707749329782832 35 26.26066299535687 24.11006730734622 33.831839614152535 15.797430083144368 36 30.377558920437153 33.960497650384404 21.06912010946868 14.592823319709764 37 33.76139188069594 23.837397788264113 24.12557184539462 18.27563848564533 38 30.642168783211744 27.63037017022426 22.076916148788616 19.650544897775376 39 36.619286962474554 20.684933974670773 23.774523050316166 18.921256012538507 40 34.28427255852512 23.71461010290328 21.907044639478094 20.094072699093516 41 35.2095160854285 17.885915112192485 22.7845363611787 24.120032441200323 42 29.563366818763864 21.660324273630675 24.879614767255216 23.896694140350245 43 26.790608770917483 20.216027120600117 24.31477207155222 28.67859203693018 44 30.454619227093215 11.645898328314068 25.533334535552633 32.36614790904009 45 32.051952737440246 14.461982502029405 24.03535672409128 29.45070803643907 46 30.88816323585127 19.482581320247526 25.677894243085746 23.95136120081546 47 27.608546576677373 16.19807268921211 31.63821416970441 24.555166564406107 48 32.98548422040875 16.007077345273345 27.06542933487398 23.942009099443922 49 30.126851801728648 16.23089554124037 25.904473195113585 27.737779461917395 50 18.486894360603653 14.723084699256264 28.799913351144486 37.9901075889956 51 20.022746967071058 14.321562680531486 33.8514586943963 31.804231658001154 52 18.839506618749212 18.494573167428168 37.38645188088057 25.279468332942045 53 17.423106792609584 18.30627246293052 39.02364138778017 25.246979356679734 54 17.767377988182787 17.957103879442748 33.09000234898723 31.185515783387242 55 19.867776954896048 15.897472950271851 32.14176044507867 32.092989649753434 56 20.680060706800607 18.056659680566597 20.963720459637205 40.29955915299559 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 3.5 14 6.0 15 11.5 16 17.0 17 25.5 18 34.0 19 82.5 20 131.0 21 201.0 22 208.0 23 145.0 24 215.0 25 285.0 26 313.0 27 341.0 28 475.0 29 686.5 30 764.0 31 778.0 32 792.0 33 749.0 34 706.0 35 669.5 36 633.0 37 678.5 38 564.5 39 405.0 40 302.5 41 200.0 42 250.0 43 300.0 44 315.5 45 331.0 46 446.0 47 835.0 48 1109.0 49 975.0 50 841.0 51 836.5 52 832.0 53 925.0 54 1626.0 55 2234.0 56 2552.0 57 2870.0 58 3047.5 59 3225.0 60 4109.0 61 4993.0 62 4959.5 63 5022.5 64 5119.0 65 7236.5 66 9354.0 67 7049.5 68 4745.0 69 3830.5 70 2916.0 71 2298.5 72 1321.5 73 962.0 74 794.0 75 626.0 76 488.5 77 351.0 78 277.5 79 171.0 80 138.0 81 136.5 82 135.0 83 102.0 84 69.0 85 67.0 86 65.0 87 59.5 88 42.5 89 31.0 90 17.5 91 4.0 92 2.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.03945622152874924 26 0.0681516553678396 27 0.10222748305175938 28 0.13271638150579287 29 0.1667922091897127 30 0.20266150148857562 31 0.22238961225295026 32 0.2582589045518132 33 0.30668244915527815 34 0.3210301660748234 35 0.34434520606908425 36 0.38918182144266295 37 0.42325764912658276 38 0.43581190143118476 39 0.4465726891208437 40 0.48244198141970657 41 0.48961583987947915 42 0.5577674952473187 43 0.5416263137128304 44 0.5470067075576599 45 0.5792890706266365 46 0.5900498583162954 47 0.6169518275404426 48 0.6635819075289645 49 0.6079845044657268 50 0.6492341906094193 51 0.6564080490691918 52 0.6904838767531116 53 0.6976577352128843 54 0.7442878152014061 55 0.7120054521324294 56 0.7353204921266904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 55758.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.36722981455576 #Duplication Level Percentage of deduplicated Percentage of total 1 71.0185243725106 20.14598801965637 2 10.640450148574319 6.036801893898634 3 4.817601315040779 4.0998601097600345 4 2.8640070809888094 3.2497578822769824 5 2.0737181513561356 2.9412819685067615 6 1.36561927040526 2.3243301409663184 7 1.2454953531010937 2.4731877040066 8 0.9230574698109629 2.094766670253596 9 0.6954542580767529 1.7755299687937158 >10 3.749130682177404 19.720936905914847 >50 0.3161155718530695 6.169518275404426 >100 0.24024783460833282 13.085117830625201 >500 0.03793386862236833 7.164891136697873 >1k 0.012644622874122779 8.71803149323864 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 3198 5.735499838588185 No Hit CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 1663 2.9825316546504537 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 840 1.5065102765522436 No Hit CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 700 1.255425230460203 No Hit CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 659 1.181893181247534 No Hit CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 647 1.160371605868216 No Hit CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC 579 1.0384160120520822 No Hit CCGCTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 570 1.022274830517594 No Hit CCGCTAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC 428 0.7676028551956671 No Hit CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 416 0.7460812798163493 No Hit CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 402 0.7209727752071452 No Hit CCGCTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 372 0.6671688367588507 No Hit CCGCTAGGGGGGGCTGGAGAGATGGTTCAATGGATAAGAGCACTGACTGCTCTTCC 359 0.6438537967645899 No Hit CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 346 0.620538756770329 No Hit CCGCTAGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT 346 0.620538756770329 No Hit CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 290 0.5201047383335127 No Hit CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 269 0.48244198141970657 No Hit CCGCTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 268 0.4806485168047634 No Hit CCGCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 208 0.37304063990817465 No Hit CCGCTAGGGCCTTTTCCTGTGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 190 0.34075827683919796 No Hit CCGCTAGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTC 172 0.3084759137702213 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC 160 0.28695433839090356 No Hit CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.2744000860863015 No Hit CCGCTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 151 0.2708131568564152 No Hit CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGA 148 0.26543276301158575 No Hit CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 146 0.2618458337816995 No Hit CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA 144 0.2582589045518132 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 144 0.2582589045518132 No Hit CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG 141 0.25287851070698375 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCA 134 0.2403242584023817 No Hit CCGCTAGGGGCCACAGCCATCTCCAGGACTGAATTATGTTCCAAAAAAAAGCATCC 130 0.23315039994260914 No Hit CCGCTAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 130 0.23315039994260914 No Hit CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT 126 0.22597654148283655 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCC 123 0.2205961476380071 No Hit CCGCTAGGGGGCCGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGG 123 0.2205961476380071 No Hit CCGCTAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT 123 0.2205961476380071 No Hit CCGCTAGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 122 0.21880268302306394 No Hit CCGCTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 121 0.2170092184081208 No Hit CCGCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 121 0.2170092184081208 No Hit CCGCTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 120 0.21521575379317764 No Hit CCGCTAGGGGGGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTTTGCCCGCCGTG 116 0.20804189533340506 No Hit CCGCTAGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCG 114 0.20445496610351876 No Hit CCGCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 113 0.20266150148857562 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTCTTCT 113 0.20266150148857562 No Hit CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACA 107 0.19190071379891677 No Hit CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA 107 0.19190071379891677 No Hit CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 99 0.17755299687937157 No Hit CCGCTAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGAT 94 0.16858567380465583 No Hit CCGCTAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC 92 0.16499874457476954 No Hit CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC 90 0.16141181534488325 No Hit CCGCTAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG 86 0.15423795688511066 No Hit CCGCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCC 85 0.15244449227016751 No Hit CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA 84 0.15065102765522434 No Hit CCGCTAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC 84 0.15065102765522434 No Hit CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTC 84 0.15065102765522434 No Hit CCGCTAGGGTAGAAGGGGGTTTTCTCAACCTCTGGCTGCTGAGAGCGATACTTCAG 82 0.14706409842533807 No Hit CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 82 0.14706409842533807 No Hit CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 81 0.1452706338103949 No Hit CCGCTAGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCC 80 0.14347716919545178 No Hit CCGCTAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA 78 0.1398902399655655 No Hit CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 77 0.13809677535062231 No Hit CCGCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 77 0.13809677535062231 No Hit CCGCTAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC 76 0.1363033107356792 No Hit CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTAAGTAAGAGCACCCGACTGCTCTT 76 0.1363033107356792 No Hit CCGCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 76 0.1363033107356792 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC 74 0.13271638150579287 No Hit CCGCTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT 70 0.12554252304602032 No Hit CCGCTAGGGGAGCTGGCTGGTCGGGGACAGGAAAGAAGGAAGAACTAACTCAGATA 69 0.12374905843107716 No Hit CCGCTAGGGGGGGGCTGGAGAGATGGTTCAATGGATAAGAGCACTGACTGCTCTTC 67 0.12016212920119085 No Hit CCGCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 67 0.12016212920119085 No Hit CCGCTAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG 65 0.11657519997130457 No Hit CCGCTAGGGGAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGATTGCTCTTCC 64 0.11478173535636141 No Hit CCGCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 64 0.11478173535636141 No Hit CCGCTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 63 0.11298827074141828 No Hit CCGCTAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 63 0.11298827074141828 No Hit CCGCTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC 63 0.11298827074141828 No Hit CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCCCC 62 0.11119480612647513 No Hit CCGCTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 61 0.10940134151153197 No Hit CCGCTAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 60 0.10760787689658882 No Hit CCGCTAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC 60 0.10760787689658882 No Hit CCGCTAGGGAGTCTTAGCCCCAGGGCAAATTCTTGCTGCAGGACAGATAGTTTGGT 60 0.10760787689658882 No Hit CCGCTAGGGGGGGGCTGGCGAGATGGCTCAGAGGTTAAGAGCGCCGACTGCTCTTC 59 0.10581441228164569 No Hit CCGCTAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG 58 0.10402094766670253 No Hit CCGCTAGGGGCTCCGCCGTCTGCCACTGCCGCCTGGGCTCACTGAGTGGTTCATCT 58 0.10402094766670253 No Hit CCGCTAGGGGAGATTCTAAGGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAGA 57 0.10222748305175938 No Hit CCGCTAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC 56 0.10043401843681625 No Hit CCGCTAGGGGCTCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCC 56 0.10043401843681625 No Hit CCGCTAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA 56 0.10043401843681625 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 56 0.10043401843681625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0017934646149431472 0.0 0.0 0.0 0.0 23 0.0017934646149431472 0.0 0.0 0.0 0.0 24 0.0035869292298862944 0.0 0.0 0.0 0.0 25 0.0035869292298862944 0.0 0.0 0.0 0.0 26 0.0035869292298862944 0.0 0.0 0.0 0.0 27 0.0035869292298862944 0.0 0.0 0.0 0.0 28 0.007173858459772589 0.0 0.0 0.0 0.0 29 0.008967323074715736 0.0 0.0 0.0 0.0 30 0.01255425230460203 0.0 0.0 0.0 0.0 31 0.01255425230460203 0.0 0.0 0.0 0.0 32 0.01255425230460203 0.0 0.0 0.0 0.0 33 0.016141181534488326 0.0 0.0 0.0 0.0 34 0.017934646149431473 0.0 0.0 0.0 0.0 35 0.023315039994260912 0.0 0.0 0.0 0.0 36 0.02510850460920406 0.0 0.0 0.0 0.0 37 0.02510850460920406 0.0 0.0 0.0 0.0 38 0.028695433839090356 0.0 0.0 0.0 0.0 39 0.030488898454033502 0.0 0.0 0.0 0.0 40 0.03228236306897665 0.0 0.0 0.0 0.0 41 0.0340758276839198 0.0 0.0 0.0 0.0 42 0.035869292298862945 0.0 0.0 0.0 0.0 43 0.035869292298862945 0.0 0.0 0.0 0.0 44 0.035869292298862945 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTATG 25 1.991538E-5 50.29295 50 AACGCAC 15 0.008197418 50.292946 49 ACTGAGA 15 0.008197418 50.292946 50 CCAGCAG 15 0.008197418 50.292946 50 ACGCACC 15 0.008197418 50.292946 50 AGAGGAC 15 0.008197418 50.292946 50 TGATACA 45 1.2369128E-10 50.292946 50 GATCTCA 15 0.008197418 50.292946 49 ATGCAGA 15 0.008197418 50.292946 49 TAGAGGA 15 0.008197418 50.292946 49 CGCCGTG 15 0.008197418 50.292946 50 CTTCCAA 15 0.008197418 50.292946 50 TCCCCTA 15 0.008197418 50.292946 49 CAAGAAA 20 4.0493303E-4 50.247517 48 GGATCTC 15 0.008226791 50.247517 48 GATGCAG 15 0.008226791 50.247517 48 GATCTTC 15 0.008226791 50.247517 48 TTTGGTA 20 4.0493303E-4 50.247517 48 CGACTGA 15 0.008226791 50.247517 48 TAAACGC 15 0.008256244 50.202168 47 >>END_MODULE