FastQCFastQC Report
Tue 31 May 2016
SRR212663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59212
Sequences flagged as poor quality0
Sequence length56
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC34175.7707897047895695No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC17953.031480105384044No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA7921.337566709450787No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7921.337566709450787No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7261.2261028169965547No Hit
CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA6841.155171249071134No Hit
CCGCTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG6681.1281496993852598No Hit
CCGCTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC6161.0403296629061678No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC5910.9981084915219887No Hit
CCGCTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4310.7278929946632439No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4200.7093156792542052No Hit
CCGCTAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC4160.7025602918327366No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3810.6434506518948861No Hit
CCGCTAGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT3810.6434506518948861No Hit
CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3490.5894075525231371No Hit
CCGCTAGGGGGGGCTGGAGAGATGGTTCAATGGATAAGAGCACTGACTGCTCTTCC3460.5843410119570357No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2840.47963250692427206No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2650.4475444166722961No Hit
CCGCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2370.4002567047220158No Hit
CCGCTAGGGCCTTTTCCTGTGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2140.3614132270485712No Hit
CCGCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2060.347902452205634No Hit
CCGCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1700.2871039654124164No Hit
CCGCTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1700.2871039654124164No Hit
CCGCTAGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTC1660.28034857799094776No Hit
CCGCTAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1600.27021549685874485No Hit
CCGCTAGGGGGGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTTTGCCCGCCGTG1590.2685266500033777No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGA1530.25839356887117476No Hit
CCGCTAGGGGCCACAGCCATCTCCAGGACTGAATTATGTTCCAAAAAAAAGCATCC1470.24826048773897183No Hit
CCGCTAGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1450.2448827940282375No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA1430.2415051003175032No Hit
CCGCTAGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCG1410.23812740660676893No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT1400.23643855975140177No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCC1400.23643855975140177No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC1360.2296831723299331No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCA1340.22630547861919884No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.22630547861919884No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTCTTCT1340.22630547861919884No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC1330.22461663176383168No Hit
CCGCTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1320.22292778490846452No Hit
CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1310.22123893805309736No Hit
CCGCTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1290.217861244342363No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1290.217861244342363No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1240.20941701006552726No Hit
CCGCTAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1210.2043504694994258No Hit
CCGCTAGGGGGCCGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGG1200.20266162264405865No Hit
CCGCTAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC1070.1807066135242856No Hit
CCGCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC1070.1807066135242856No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTAAGTAAGAGCACCCGACTGCTCTT1040.17564007295818415No Hit
CCGCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1040.17564007295818415No Hit
CCGCTAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT1030.173951226102817No Hit
CCGCTAGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1010.1705735323920827No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACA1000.16888468553671554No Hit
CCGCTAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGAT960.1621292981152469No Hit
CCGCTAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC910.15368506383841113No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG900.15199621698304397No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT890.1503073701276768No Hit
CCGCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCC890.1503073701276768No Hit
CCGCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC870.14692967641694252No Hit
CCGCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG860.14524082956157536No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA830.14017428899547388No Hit
CCGCTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG790.13341890157400527No Hit
CCGCTAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG790.13341890157400527No Hit
CCGCTAGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCC780.1317300547186381No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC780.1317300547186381No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC780.1317300547186381No Hit
CCGCTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT770.13004120786327097No Hit
CCGCTAGGGGGGGGCTGGCGAGATGGCTCAGAGGTTAAGAGCGCCGACTGCTCTTC750.12666351415253663No Hit
CCGCTAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA730.12328582044180234No Hit
CCGCTAGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGA730.12328582044180234No Hit
CCGCTAGGGGCTCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCC730.12328582044180234No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA720.12159697358643518No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGTTCAATGGATAAGAGCACTGACTGCTCTTC710.11990812673106802No Hit
CCGCTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA710.11990812673106802No Hit
CCGCTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC710.11990812673106802No Hit
CCGCTAGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCCGCCCCCCCCTATC710.11990812673106802No Hit
CCGCTAGGGAGCCAAAGCAACACCTCGCACTCCTGCCCCAGCATGCAGAAGGACGC700.11821927987570088No Hit
CCGCTAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG700.11821927987570088No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTC700.11821927987570088No Hit
CCGCTAGGGGGTCTGAGTCCTGGCCGAGGAACTTCTGTTGGTGTTTGGGGCCGCAG690.11653043302033371No Hit
CCGCTAGGGTAGAAGGGGGTTTTCTCAACCTCTGGCTGCTGAGAGCGATACTTCAG690.11653043302033371No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCCCC680.11484158616496655No Hit
CCGCTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAA670.11315273930959942No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC650.10977504559886508No Hit
CCGCTAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA650.10977504559886508No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCCGC640.10808619874349795No Hit
CCGCTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG620.10470850503276363No Hit
CCGCTAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC620.10470850503276363No Hit
CCGCTAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC610.10301965817739647No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG600.10133081132202933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAATT150.00828200350.16652341
GGATGCT150.00828200350.16652347
TCCCATC150.00828200350.16652347
AAACCAG204.083816E-450.16652344
CCTATTG150.00828200350.16652340
CATTGGA150.00828200350.16652343
GTCATCA150.00828200350.16652346
CACATCC150.00828200350.16652345
AAACATT150.00828200350.16652340
GTCGCTG204.083816E-450.16652342
AATTCCC150.00828200350.16652344
GCAGGTC150.00828200350.16652340
GGTCTGC204.083816E-450.16652343
GTTCCCA150.00828200350.16652349
CATCCCC150.00828200350.16652347
TTATTGT204.083816E-450.16652350
ATTGGAT150.00828200350.16652344
TGTCATC150.00828200350.16652345
TGCTGGA150.00828200350.16652350
CGTAGTT150.00828200350.16652345
Produced by FastQC (version 0.11.5)