SRR212662_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212662_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117666
Sequences flagged as poor quality	0
Sequence length	58
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

$Per base quality graph$

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	2276	1.9342885795386942	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	2141	1.8195570513147383	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	1551	1.3181377798174494	No Hit
GCGAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	1251	1.0631788282086585	No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	752	0.639097105366036	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	626	0.5320143456903439	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	579	0.4920707766049666	No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC	536	0.4555266602077066	No Hit
GCGAATGGGACACTCGTGGGCACGATCTCATGGTGTTCTAACTATGAGGGAAATTTAC	527	0.4478778916594428	No Hit
GCGAATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	480	0.40793432257406553	No Hit
GCGAATGGGGGTTTGGTCTTCTCATTGTGTCCTGGATTTCCTGGATGTTTTGAGTTAG	409	0.34759403735998506	No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC	391	0.3322965002634576	No Hit
GCGAATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA	319	0.2711063518773477	No Hit
GCGAATGGGGCTGCCCGTGCAGCCAGAGGATCCAGCACCTCTCTTGGGGCTTCTCCGT	313	0.26600717284517195	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	307	0.2609079938129961	No Hit
GCGAATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	302	0.2566586779528496	No Hit
GCGAATGGGAGTTTGTGTTTGGTTAGTGGATATAGGTGAAATAGAATGAAAGTAATGT	260	0.22096442472761885	No Hit
GCGAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	256	0.21756497203950162	No Hit
GCGAATGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA	251	0.2133156561793551	No Hit
GCGAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	246	0.2090663403192086	No Hit
GCGAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	245	0.2082164771471793	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG	245	0.2082164771471793	No Hit
GCGAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	237	0.20141757177094488	No Hit
GCGAATGGGAAGACAGCCTTTGTCACCTTTGTCCAGTTCCCCAGATCTTCTGGTCTTC	211	0.17932112929818297	No Hit
GCGAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	208	0.1767715397820951	No Hit
GCGAATGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC	198	0.16827290806180206	No Hit
GCGAATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	195	0.16572331854571412	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG	185	0.15722468682542112	No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	178	0.15127564462121598	No Hit
GCGAATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG	177	0.1504257814491867	No Hit
GCGAATGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA	172	0.14617646558904016	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG	165	0.14022742338483504	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA	163	0.13852769704077644	No Hit
GCGAATGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC	162	0.13767783386874713	No Hit
GCGAATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	162	0.13767783386874713	No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA	156	0.1325786548365713	No Hit
GCGAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA	153	0.1300290653204834	No Hit
GCGAATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	152	0.1291792021484541	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	147	0.12492988628830758	No Hit
GCGAATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	146	0.12408002311627828	No Hit
GCGAATGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA	145	0.12323015994424896	No Hit
GCGAATGGGCTCTGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTC	142	0.12068057042816108	No Hit
GCGAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	142	0.12068057042816108	No Hit
GCGAATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	139	0.11813098091207315	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCAACTGCTCTTCTG	138	0.11728111774004385	No Hit
GCGAATGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCTT	138	0.11728111774004385	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG	136	0.11558139139598525	No Hit
GCGAATGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC	133	0.11303180187989734	No Hit
GCGAATGGGGGAATTCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	129	0.10963234919178011	No Hit
GCGAATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	128	0.10878248601975081	No Hit
GCGAATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	127	0.10793262284772152	No Hit
GCGAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	125	0.10623289650366291	No Hit
GCGAATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA	124	0.10538303333163361	No Hit
GCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	124	0.10538303333163361	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA	123	0.1045331701596043	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCCCTTCCG	122	0.103683306987575	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCAA	119	0.1011337174714871	No Hit
GCGAATGGGGACTGAAATTGAATTCACCAACATCTGCCCTAGGCTTTCAGACCTGGGG	118	0.1002838542994578	No Hit
GCGAATGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG	118	0.1002838542994578	No Hit
GCGAATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA	118	0.1002838542994578	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACC	15	0.0071736444	52.055748	49
GTTTATG	15	0.0071736444	52.055748	51
CTCCAGG	15	0.0071736444	52.055748	49
TAGTTTA	15	0.0071736444	52.055748	49
GTAATGT	15	0.0071736444	52.055748	52
AAACCTA	15	0.0071736444	52.055748	51
AACCTAC	15	0.0071736444	52.055748	52
TTACGGC	15	0.0071736444	52.055748	52
AGTAATG	15	0.0071736444	52.055748	51
CAACCAA	25	1.6356385E-5	52.055744	49
CAATGCG	25	1.6356385E-5	52.055744	52
TATTTCT	15	0.0071978304	52.011482	43
TTCTAAC	60	0.0	52.011482	36
TCGTTTC	15	0.0071978304	52.011482	47
ACTATGA	60	0.0	52.011482	41
TCGTTGC	25	1.6439026E-5	52.011482	46
AGATAGG	15	0.0071978304	52.011482	46
ACTGAGT	30	7.921353E-7	52.011482	45
TCACAAT	30	7.921353E-7	52.011482	41
CAGCATC	15	0.0071978304	52.011482	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content