FastQCFastQC Report
Tue 31 May 2016
SRR212661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117246
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT21771.8567797622093718No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA20651.761254115278986No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG14411.2290397966668372No Hit
GCGAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG12211.0414001330535796No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6950.5927707555055183No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA6390.5450079320403254No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA5420.4622758985381164No Hit
GCGAATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4970.42389505825358653No Hit
GCGAATGGGACACTCGTGGGCACGATCTCATGGTGTTCTAACTATGAGGGAAATTTAC4930.42048342800607275No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC4410.37613223478839364No Hit
GCGAATGGGGGTTTGGTCTTCTCATTGTGTCCTGGATTTCCTGGATGTTTTGAGTTAG4130.3522508230557972No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC3750.31984033570441633No Hit
GCGAATGGGGCTGCCCGTGCAGCCAGAGGATCCAGCACCTCTCTTGGGGCTTCTCCGT2960.25246063831601934No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2930.249901915630384No Hit
GCGAATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA2800.23881411732596422No Hit
GCGAATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2770.2362553946403289No Hit
GCGAATGGGAGTTTGTGTTTGGTTAGTGGATATAGGTGAAATAGAATGAAAGTAATGT2700.23028504170717976No Hit
GCGAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2690.2294321341453013No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG2550.21749142827900314No Hit
GCGAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2510.21407979803148933No Hit
GCGAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2480.21152107534585401No Hit
GCGAATGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA2300.19616873923204203No Hit
GCGAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2230.19019838629889294No Hit
GCGAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.17314023506132406No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2030.17314023506132406No Hit
GCGAATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2000.17058151237568872No Hit
GCGAATGGGAAGACAGCCTTTGTCACCTTTGTCCAGTTCCCCAGATCTTCTGGTCTTC1990.16972860481381027No Hit
GCGAATGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1980.16887569725193183No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1860.15864080650939053No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1690.14414137795745696No Hit
GCGAATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1690.14414137795745696No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1690.14414137795745696No Hit
GCGAATGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC1570.13390648721491566No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA1470.12537741159613122No Hit
GCGAATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1470.12537741159613122No Hit
GCGAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1470.12537741159613122No Hit
GCGAATGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCTT1460.12452450403425276No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1440.12281868891049587No Hit
GCGAATGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1440.12281868891049587No Hit
GCGAATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1420.12111287378673899No Hit
GCGAATGGGCTCTGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTC1410.12025996622486056No Hit
GCGAATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1400.11940705866298211No Hit
GCGAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1390.11855415110110366No Hit
GCGAATGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1360.11599542841546832No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG1330.113436705729833No Hit
GCGAATGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGTCCTTCCG1320.11258379816795455No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC1310.11173089060607612No Hit
GCGAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.10746635279668389No Hit
GCGAATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1240.10576053767292701No Hit
GCGAATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1240.10576053767292701No Hit
GCGAATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1230.10490763011104856No Hit
GCGAATGGGGACTGAAATTGAATTCACCAACATCTGCCCTAGGCTTTCAGACCTGGGG1220.10405472254917013No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCCCTTCCG1220.10405472254917013No Hit
GCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1210.10320181498729168No Hit
GCGAATGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1200.10234890742541324No Hit
GCGAATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1180.10064309230165636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC353.7427526E-852.17488526
CCCTACA203.3783668E-452.17488527
TACATCA203.3783668E-452.17488524
TCTCATG700.052.17488526
TAGTGGA353.7427526E-852.17488524
CAGAGAG203.3783668E-452.17488527
ACGCGAT251.6135868E-552.1748824
CGCGATC251.6135868E-552.1748825
ACCTCCG150.007108964552.1748827
TATTGTG150.007108964552.1748825
TTAAGGG150.007108964552.1748824
AGACCTC150.007108964552.1748825
TAAGGGT150.007108964552.1748825
ATTCTTC150.007108964552.1748827
CCCTAGC203.385527E-452.1525321
CATTGTG353.7554855E-852.1525323
AATTCAC203.385527E-452.1525321
ATACATC203.385527E-452.1525323
CCAGACC150.00712102952.15252723
ACGATCT750.052.15252722
Produced by FastQC (version 0.11.5)