FastQCFastQC Report
Tue 31 May 2016
SRR212660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117093
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT19241.6431383601069236No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA18151.5500499602879763No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG14361.2263756159633794No Hit
GCGAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10670.9112414918056588No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA6190.5286396283296183No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5770.4927707036287396No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC5760.49191668161205193No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA5640.4816684174118009No Hit
GCGAATGGGACACTCGTGGGCACGATCTCATGGTGTTCTAACTATGAGGGAAATTTAC4390.37491566532585213No Hit
GCGAATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC3830.32709043239134705No Hit
GCGAATGGGGGTTTGGTCTTCTCATTGTGTCCTGGATTTCCTGGATGTTTTGAGTTAG3470.2963456397905938No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC3160.2698709572732785No Hit
GCGAATGGGGCTGCCCGTGCAGCCAGAGGATCCAGCACCTCTCTTGGGGCTTCTCCGT2690.22973192248896177No Hit
GCGAATGGGAGTTTGTGTTTGGTTAGTGGATATAGGTGAAATAGAATGAAAGTAATGT2640.22546181240552382No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2620.22375376837214864No Hit
GCGAATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2590.22119170232208588No Hit
GCGAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2570.2194836582887107No Hit
GCGAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2470.2109434381218348No Hit
GCGAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2360.20154919593827128No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG2300.19642506383814573No Hit
GCGAATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA2100.17934462350439392No Hit
GCGAATGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA2070.1767825574543312No Hit
GCGAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.16141016115395454No Hit
GCGAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1820.1554320070371414No Hit
GCGAATGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1790.15286994098707865No Hit
GCGAATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1760.15030787493701586No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1730.14774580888695313No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1710.14603776485357792No Hit
GCGAATGGGAAGACAGCCTTTGTCACCTTTGTCCAGTTCCCCAGATCTTCTGGTCTTC1710.14603776485357792No Hit
GCGAATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1710.14603776485357792No Hit
GCGAATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1640.1400596107367648No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1630.13920558872007718No Hit
GCGAATGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC1520.12981134653651372No Hit
GCGAATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1490.12724928048645093No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1450.12383319241970059No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGAATGGGAAGCAGTGG1420.12127112636963781No Hit
GCGAATGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1400.11956308233626264No Hit
GCGAATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1390.11870906031957504No Hit
GCGAATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1380.11785503830288745No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA1360.11614699426951228No Hit
GCGAATGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1360.11614699426951228No Hit
GCGAATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1330.1135849282194495No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA1250.10675275208594877No Hit
GCGAATGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1240.10589873006926118No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG1230.1050447080525736No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1230.1050447080525736Illumina Single End Adapter 2 (96% over 25bp)
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCAACTGCTCTTCTG1210.10333666401919843No Hit
GCGAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1180.10077459796913564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG150.006982096552.412789
GGATGTA150.006982096552.412788
GGACTAA203.3032423E-452.412788
GGGTAAT150.006982096552.412788
AAGACAG458.367351E-1152.4127810
GAGAATT203.3032423E-452.4127810
GAACGCA251.5706493E-552.412789
GGGCACA251.5706493E-552.412788
ACACTCG650.052.4127810
TGGGACG401.7425918E-952.412786
GATTCCT150.006982096552.4127810
GACTAAG150.006982096552.412789
ACCGTAG1050.052.03086539
TACCGTA1050.052.03086538
GTACCGG150.0071870952.0308639
CGGCCGA150.0071870952.0308629
GTTAGCG251.6401995E-552.0308638
CGACACC150.0071870952.0308628
TGTGCAT150.0071870952.0308638
CTAACTA700.052.0308638
Produced by FastQC (version 0.11.5)