FastQCFastQC Report
Tue 31 May 2016
SRR212659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120127
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC25382.11276399144239No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT23211.9321218377217446No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC20981.746484970073339No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16111.341080689603503No Hit
GCGAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA10730.8932213407477086No Hit
GCGAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8060.6709565709623981No Hit
GCGAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7730.6434856443597193No Hit
GCGAATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC7210.6001981236524678No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT6390.5319370333064174No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC6010.5003038450972721No Hit
GCGAATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA5180.43121030242992836No Hit
GCGAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5000.41622616064664897No Hit
GCGAATGGGACACTCGTGGGCACGATCTCATGGTGTTCTAACTATGAGGGAAATTT4670.38875523404397017No Hit
GCGAATGGGGGTTTGGTCTTCTCATTGTGTCCTGGATTTCCTGGATGTTTTGAGTT3550.29552057405912074No Hit
GCGAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3530.2938556694165342No Hit
GCGAATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2900.2414111731750564No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2800.23308664996212342No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2760.22975684067695024No Hit
GCGAATGGGGCTGCCCGTGCAGCCAGAGGATCCAGCACCTCTCTTGGGGCTTCTCC2690.22392967442789713No Hit
GCGAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.20811308032332448No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC2420.2014534617529781No Hit
GCGAATGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC2320.19312893854004512No Hit
GCGAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2310.19229648621875184No Hit
GCGAATGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT2300.19146403389745853No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC2260.18813422461228532No Hit
GCGAATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA2230.18563686764840542No Hit
GCGAATGGGAGTTTGTGTTTGGTTAGTGGATATAGGTGAAATAGAATGAAAGTAAT2150.17897724907805904No Hit
GCGAATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2130.17731234443547245No Hit
GCGAATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2100.17481498747159258No Hit
GCGAATGGGGCCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCC2040.16982027354383278No Hit
GCGAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1990.1656580119373663No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1900.1581659410457266No Hit
GCGAATGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT1800.14984141783279362No Hit
GCGAATGGGAAGACAGCCTTTGTCACCTTTGTCCAGTTCCCCAGATCTTCTGGTCT1750.14567915622632713No Hit
GCGAATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1730.14401425158374054No Hit
GCGAATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1690.14068444229856736No Hit
GCGAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1690.14068444229856736No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC1610.13402482372822097No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC1600.13319237140692766No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1590.13235991908563438No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1530.12736520515787458No Hit
GCGAATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC1490.12403539587270138No Hit
GCGAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1420.11820822962364831No Hit
GCGAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1420.11820822962364831No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC1390.11571087265976841No Hit
GCGAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.11404596801718181No Hit
GCGAATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1330.11071615873200862No Hit
GCGAATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1330.11071615873200862No Hit
GCGAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1290.10738634944683542No Hit
GCGAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.10655389712554214No Hit
GCGAATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG1270.10572144480424885No Hit
GCGAATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1240.10322408784036895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATAT150.0082384350.26532449
CGAAACC150.0082384350.26532449
CATGCAG150.0082384350.26532448
ACGCACC204.0612443E-450.26532450
ATTCGTA150.0082384350.26532450
TGAAAAC252.0121313E-550.26532450
TTGAGTT252.0121313E-550.26532450
TATCTCA252.0121313E-550.26532448
GTAGAAT150.0082384350.26532449
CCATATG150.0082384350.26532450
TCTCGCA150.0082384350.26532449
AACGCAC204.0612443E-450.26532449
AAATTCG150.0082384350.26532448
CCCTTCG150.0082384350.26532448
CTCGCAA150.0082384350.26532450
CATCGAA204.0612443E-450.26532450
TTTGATA204.0612443E-450.26532448
CAATTAT204.0781102E-450.22315644
TTATCTC204.0781102E-450.22315647
TTTCAGA204.0781102E-450.22315645
Produced by FastQC (version 0.11.5)