FastQCFastQC Report
Tue 31 May 2016
SRR212658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112689
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC23942.12443095599393No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT21571.9141176157388922No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC19271.7100160619048888No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC17091.5165632847926593No Hit
GCGAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9640.8554517299825183No Hit
GCGAATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC8970.7959960599526129No Hit
GCGAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8410.7463017685843338No Hit
GCGAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8410.7463017685843338No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT6140.5448624089307741No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC5640.500492505923382No Hit
GCGAATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA5600.49694291368279064No Hit
GCGAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4850.43038805917170264No Hit
GCGAATGGGACACTCGTGGGCACGATCTCATGGTGTTCTAACTATGAGGGAAATTT4320.3833559619838671No Hit
GCGAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3690.3274498841945531No Hit
GCGAATGGGGGTTTGGTCTTCTCATTGTGTCCTGGATTTCCTGGATGTTTTGAGTT3320.2946161559690831No Hit
GCGAATGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3160.2804177870067176No Hit
GCGAATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3150.27953038894656973No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2720.24137227236021264No Hit
GCGAATGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC2430.21563772861592526No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC2400.21297553443548173No Hit
GCGAATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA2330.20676374801444683No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC2320.205876349954299No Hit
GCGAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2310.20498895189415117No Hit
GCGAATGGGGCTGCCCGTGCAGCCAGAGGATCCAGCACCTCTCTTGGGGCTTCTCC2290.2032141557738555No Hit
GCGAATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA2260.20055196159341196No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2230.19788976741296843No Hit
GCGAATGGGGCCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCC2220.1970023693528206No Hit
GCGAATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2170.1925653790520814No Hit
GCGAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG2160.19167798099193356No Hit
GCGAATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2120.1881283887513422No Hit
GCGAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.17836701008971592No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1990.17659221396942026No Hit
GCGAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1960.17393001978897674No Hit
GCGAATGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT1950.17304262172882892No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1930.1712678256085332No Hit
GCGAATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1850.1641686411273505No Hit
GCGAATGGGAGTTTGTGTTTGGTTAGTGGATATAGGTGAAATAGAATGAAAGTAAT1760.15618205858601994No Hit
GCGAATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC1530.1357719032026196No Hit
GCGAATGGGAAGACAGCCTTTGTCACCTTTGTCCAGTTCCCCAGATCTTCTGGTCT1450.12867271872143687No Hit
GCGAATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1430.1268979226011412No Hit
GCGAATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC1370.12157353424025416No Hit
GCGAATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1360.12068613618010632No Hit
GCGAATGGGGTCTATCGCCCGAGTCCTCACTGTTATTAACCAGACTCAAAAAGAAA1310.1162491458793671No Hit
GCGAATGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA1300.11536174781921926No Hit
GCGAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1280.11358695169892358No Hit
GCGAATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1270.11269955363877576No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC1270.11269955363877576No Hit
GCGAATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG1260.1118121555786279No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1250.11092475751848006No Hit
GCGAATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA1240.11003735945833222No Hit
GCGAATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1180.10471297109744518No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1170.10382557303729734No Hit
GCGAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.1029381749771495No Hit
GCGAATGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC1150.10205077691700166No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC1140.10116337885685382No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1130.10027598079670598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTAC204.0961677E-450.17552650
TTTTACG204.0961677E-450.17552650
CATCGAA301.0145304E-650.1755250
TTAGGGT150.00831022950.15312649
GTCGCGT301.0176627E-650.15312644
GCAAGCC150.00831022950.15312649
CTGTAGG150.00831022950.15312645
CTCAAAG204.1052097E-450.15312644
TCACAAT204.1052097E-450.15312641
ACCTGAC150.00831022950.15312641
TTATCTC204.1052097E-450.15312647
GACTTAA150.00831022950.15312649
GAAAGTA204.1052097E-450.15312648
CCCAAGG252.0381165E-550.15312648
TATCTCA204.1052097E-450.15312648
CGTAGCG650.050.15312641
AGATTAA204.1052097E-450.15312649
GCGTGCA355.1075403E-850.15312647
GGCCTTA150.00831022950.15312645
TCGCGTG301.0176627E-650.15312645
Produced by FastQC (version 0.11.5)