FastQCFastQC Report
Tue 31 May 2016
SRR212657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212657_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63117
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5730.9078378249916821No Hit
TTAACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG5170.8191137094602088No Hit
TTAACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4810.7620767780471188No Hit
TTAACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4490.7113772834577055No Hit
TTAACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3610.5719536733368189No Hit
TTAACTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC3370.533929052394759No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2750.43569878162777065No Hit
TTAACTGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2610.4135177527449023No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2550.4040115975093873No Hit
TTAACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2480.3929210830679532No Hit
TTAACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2350.372324413391004No Hit
TTAACTGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2150.3406372292726207No Hit
TTAACTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1890.2994438899187224No Hit
TTAACTGGGGTCAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAG1850.2931064530950457No Hit
TTAACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1760.2788472202417732No Hit
TTAACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1620.2566661913589049No Hit
TTAACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1560.2471600361233899No Hit
TTAACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1500.23765388088787487No Hit
TTAACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1440.22814772565235988No Hit
TTAACTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1340.21230413359316827No Hit
TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.20279797835765323No Hit
TTAACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1270.20121361915173408No Hit
TTAACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1270.20121361915173408No Hit
TTAACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1260.1996292599458149No Hit
TTAACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1230.19487618232805742No Hit
TTAACTGGGAAGTCTAATAGGTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTTTA1220.19329182312213825No Hit
TTAACTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1140.18061694947478493No Hit
TTAACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1080.17111079423926995No Hit
TTAACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1010.16002027979783576No Hit
TTAACTGGGCAGTTCCCACCACCTCCACAGAGCCCCCTAACAATCCCGGATATCAGGA980.15526720218007828No Hit
TTAACTGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACGG950.15051412456232077No Hit
TTAACTGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC880.1394236101208866No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC860.13625489170904825No Hit
TTAACTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT840.13308617329720995No Hit
TTAACTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC840.13308617329720995No Hit
TTAACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.13150181409129078No Hit
TTAACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA820.1299174548853716No Hit
TTAACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT770.12199565885577578No Hit
TTAACTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC760.12041129964985661No Hit
TTAACTGGGAATGCTTTGATAGCAAGCATAGAATTTCACATTTGACATGCTCTGTGGT760.12041129964985661No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA730.11565822203209912No Hit
TTAACTGGGGGGAATTCCTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGAAC670.10615206679658414No Hit
TTAACTGGGGGCCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT670.10615206679658414No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC660.10456770759066496No Hit
TTAACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG660.10456770759066496No Hit
TTAACTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG660.10456770759066496No Hit
TTAACTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG660.10456770759066496No Hit
TTAACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.10139898917882662No Hit
TTAACTGGGATGTGTGTGTGTGTAAGTATGTAGGGGTATGTGAGCTTCTGTGTGTTTG640.10139898917882662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATA203.3867126E-452.11128645
ACTGACG150.00712810352.11128651
TATGTTT150.00712810352.11128647
TCGAACT150.00712810352.11128652
GTTCAAT150.00712810352.11128644
TCAATAT150.00712810352.11128646
TTTTAGA203.3867126E-452.11128643
CAATATT150.00712810352.11128647
CCATTTG150.00712810352.11128648
TCATGAT150.00712810352.11128651
CCGAGGG203.3867126E-452.11128646
GATTAAT203.3867126E-452.11128644
TCCGAGG203.3867126E-452.11128645
TTTATGT150.00712810352.11128652
CATACCC150.00712810352.11128652
AAATTGC150.007173071652.02857241
ACCGTAG150.007173071652.02857239
GTTTTAG203.4134163E-452.02857242
CGTAGCG150.007173071652.02857241
GTGAGCT150.007173071652.02857240
Produced by FastQC (version 0.11.5)