FastQCFastQC Report
Tue 31 May 2016
SRR212656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212656_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67549
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5750.8512339190809635No Hit
TTAACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG5330.7890568328176584No Hit
TTAACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4940.731320967001732No Hit
TTAACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4360.6454573716857392No Hit
TTAACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4200.6217708626330516No Hit
TTAACTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC3130.46336733334320274No Hit
TTAACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3040.4500436720010659No Hit
TTAACTGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2770.4100726879746554No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2740.40563146752727647No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2580.3819449584745888No Hit
TTAACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2530.3745429243956239No Hit
TTAACTGGGGTCAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAG2400.3552976357903152No Hit
TTAACTGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2170.32124827902707664No Hit
TTAACTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1980.29312054952701No Hit
TTAACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1860.27535566773749426No Hit
TTAACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1700.2516691586848066No Hit
TTAACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1680.24870834505322065No Hit
TTAACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1640.2427867177900487No Hit
TTAACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1550.2294630564479119No Hit
TTAACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1430.21169817465839613No Hit
TTAACTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1400.2072569542110172No Hit
TTAACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1400.2072569542110172No Hit
TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.20429614057943124No Hit
TTAACTGGGAAGTCTAATAGGTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTTTA1240.18357044515832951No Hit
TTAACTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1190.1761684110793646No Hit
TTAACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1160.17172719063198566No Hit
TTAACTGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACGG1150.17024678381619268No Hit
TTAACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1120.16580556336881375No Hit
TTAACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1100.1628447497372278No Hit
TTAACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1060.15692312247405588No Hit
TTAACTGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1040.15396230884246992No Hit
TTAACTGGGCAGTTCCCACCACCTCCACAGAGCCCCCTAACAATCCCGGATATCAGGA1040.15396230884246992No Hit
TTAACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.14804068157929798No Hit
TTAACTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG1000.14804068157929798No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1000.14804068157929798No Hit
TTAACTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC890.13175620660557522No Hit
TTAACTGGGGGCCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT870.12879539297398926No Hit
TTAACTGGGAATGCTTTGATAGCAAGCATAGAATTTCACATTTGACATGCTCTGTGGT860.12731498615819628No Hit
TTAACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT830.12287376571081733No Hit
TTAACTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC800.1184325452634384No Hit
TTAACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.1184325452634384No Hit
TTAACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.11251091800026647No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC730.10806969755288752No Hit
TTAACTGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG730.10806969755288752No Hit
TTAACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT730.10806969755288752No Hit
TTAACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG720.10658929073709454No Hit
TTAACTGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA710.10510888392130156No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA700.1036284771055086No Hit
TTAACTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT690.10214807028971562No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA680.10066766347392264No Hit
TTAACTGGGGCTCTTTCCGAGCTGCTAGCGCTCCTGCGAACATGGTGAACGTGCCTAA680.10066766347392264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAC251.618927E-552.105552
TCGAACT203.39028E-452.10549552
GGTCATC150.007133397352.10549552
CTGTTTA150.007133397352.10549552
ACAACGG353.7502105E-852.10549552
TATGGAT150.007133397352.10549552
GTGCTCC150.007133397352.10549552
GGTTATT203.4027497E-452.06681450
CGTGCTC150.00715439952.06681451
GTTATTA203.4027497E-452.06681451
ATATCAG150.00715439952.06681450
GAGATAT150.00715439952.06681451
ATCCCGG150.00717544752.0281943
AGCCCCC150.00717544752.0281931
CCAATTA150.00717544752.0281942
AACAATC150.00717544752.0281939
CCGGATA150.00717544752.0281946
TAAACCT150.00717544752.0281931
ACAGAGC150.00717544752.0281927
ACATGCT150.00717544752.0281945
Produced by FastQC (version 0.11.5)