FastQCFastQC Report
Tue 31 May 2016
SRR212655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212655_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68621
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5570.8117048716865101No Hit
TTAACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5080.7402981594555602No Hit
TTAACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4920.716981681992393No Hit
TTAACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4060.5916556156278691No Hit
TTAACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3560.5187916235554714No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2870.4182393144955626No Hit
TTAACTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC2760.4022092362396351No Hit
TTAACTGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2530.36869179988633216No Hit
TTAACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2520.3672345200448842No Hit
TTAACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2470.35994812083764444No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2370.34537532242316493No Hit
TTAACTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2190.31914428527710176No Hit
TTAACTGGGGTCAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAG2040.2972850876553825No Hit
TTAACTGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2030.29582780781393453No Hit
TTAACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1750.25502397225339185No Hit
TTAACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1630.23753661415601637No Hit
TTAACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1550.22587837542443276No Hit
TTAACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1540.2244210955829848No Hit
TTAACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1500.21859197621719298No Hit
TTAACTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1450.21130557700995323No Hit
TTAACTGGGAAGTCTAATAGGTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTTTA1400.20401917780271348No Hit
TTAACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1280.18653181970533803No Hit
TTAACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1260.1836172600224421No Hit
TTAACTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1190.17341630113230644No Hit
TTAACTGGGCAGTTCCCACCACCTCCACAGAGCCCCCTAACAATCCCGGATATCAGGA1110.1617580624007228No Hit
TTAACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1100.16030078255927485No Hit
TTAACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1090.1588435027178269No Hit
TTAACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1070.155928943034931No Hit
TTAACTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1070.155928943034931No Hit
TTAACTGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACGG990.14427070430334737No Hit
TTAACTGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC970.14135614462045146No Hit
TTAACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.13844158493755554No Hit
TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.1369843050961076No Hit
TTAACTGGGGGCCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT930.13552702525465965No Hit
TTAACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG840.12241150668162808No Hit
TTAACTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT790.1151251074743883No Hit
TTAACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.11366782763294035No Hit
TTAACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT760.11075326795004445No Hit
TTAACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA750.10929598810859649No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC730.10638142842570059No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC720.10492414858425264No Hit
TTAACTGGGGGGAATTCCTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGAAC700.10200958890135674No Hit
TTAACTGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA700.10200958890135674No Hit
TTAACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT690.10055230905990878No Hit
TTAACTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG690.10055230905990878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCCC150.0071290752.1142952
AACCAAA150.0071290752.1142950
GTGATTT150.0071290752.1142951
CAGATCT150.0071290752.1142952
TCGAACT251.6175552E-552.11428552
CTCTCCC650.052.11428552
ATATCAG251.6175552E-552.11428550
GGCCGCT203.3877994E-452.1142850
ATTAAGC203.3877994E-452.1142851
GTTCTAG150.00714976652.07613842
CCTGACT150.00714976652.07613843
ACCTGAC150.00714976652.07613842
GTCCTGG150.00714976652.07613823
CTAATCA150.00714976652.07613823
GTCTCAA150.00714976652.07613842
TAATCAG150.00714976652.07613824
GCCACGA150.00714976652.07613843
CGTTGGA150.00714976652.07613827
AACAATC251.6245938E-552.07613439
AATCCCG251.6245938E-552.07613442
Produced by FastQC (version 0.11.5)