FastQCFastQC Report
Tue 31 May 2016
SRR212654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191135
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT25101.313207942030502No Hit
TTAACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC16200.847568472545583No Hit
TTAACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA14680.7680435294425406No Hit
TTAACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC13260.6937504904910142No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11200.5859732649697857No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC10110.5289455097182619No Hit
TTAACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA10110.5289455097182619No Hit
TTAACTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC7550.39500876343945374No Hit
TTAACTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC6330.3311795327909593No Hit
TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.31496062992125984No Hit
TTAACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6010.31443743950610825No Hit
TTAACTGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5710.29874172705156044No Hit
TTAACTGGGGTCAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAG5400.282522824181861No Hit
TTAACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA5370.2809532529364062No Hit
TTAACTGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT5090.2663039213121616No Hit
TTAACTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4800.25113139927276534No Hit
TTAACTGGGATGTGTGTGTGTGTAAGTATGTAGGTGTATGTGAGCTTCTGTGTGTT4510.2359588772333691No Hit
TTAACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4210.22026316477882124No Hit
TTAACTGGGAATGCTTTGATAGCAAGCATAGAATTTCACATTTGACATGCTCTGTG4160.21764721270306325No Hit
TTAACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4160.21764721270306325No Hit
TTAACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC3950.20666021398487977No Hit
TTAACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3900.20404426190912184No Hit
TTAACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.19828916734245428No Hit
TTAACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3480.18207026447275487No Hit
TTAACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC3380.17683836032123892No Hit
TTAACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3250.1700368849242682No Hit
TTAACTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3180.16637455201820703No Hit
TTAACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3050.1595730766212363No Hit
TTAACTGGGACTTAATCAATTTCTAACAAACTGTCCTTTTCTAAATTCTGGTTATT2980.15591074371517513No Hit
TTAACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.15591074371517513No Hit
TTAACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.15172522039396238No Hit
TTAACTGGGAAGTCTAATAGGTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTT2740.14335417375153686No Hit
TTAACTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2730.14283098333638528No Hit
TTAACTGGGATAGTTTCTACTGACAGGTTTCAGAGCTCCCAAAGATTAATTTCCAA2630.13759907918486933No Hit
TTAACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA2540.132890365448505No Hit
TTAACTGGGCAGTTCCCACCACCTCCACAGAGCCCCCTAACAATCCCGGATATCAG2460.12870484212729222No Hit
TTAACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2440.12765846129698905No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2420.12661208046668584No Hit
TTAACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTAACTGGGAAGCAGT2410.12608889005153426No Hit
TTAACTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT2360.1234729379757763No Hit
TTAACTGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC2250.11771784340910874No Hit
TTAACTGGGGGCCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC2210.11562508174850236No Hit
TTAACTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA2120.11091636801213803No Hit
TTAACTGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAAC1980.1035917022000157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCAC251.9970492E-550.34849550
CGAGCTC150.00819217250.3484950
TAGGCCG150.00819217250.3484950
ATTTTCG150.0082007450.33518249
CGCTAGT150.0082007450.33518249
GCCCGTT150.0082007450.33518249
CGGATAT150.00822648550.295347
CACAACC150.00822648550.295347
CCGGATA150.0082350850.28201746
TTCCCGG204.060714E-450.28201746
CCCGGAT150.00826090450.24221845
GTTAATA150.00826952650.22896643
ACGCTAC150.00826952650.22896644
TATACTA150.00826952650.22896644
ACGTTGA150.00828678950.2024841
CGACGCT150.00828678950.2024842
ACCAGAC150.00828678950.2024842
ACGACGC150.00828678950.2024841
CTCAACT252.0316755E-550.20247741
CGTAGCG252.0316755E-550.20247741
Produced by FastQC (version 0.11.5)