FastQCFastQC Report
Tue 31 May 2016
SRR212653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177310
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT21151.192826123738086No Hit
TTAACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC16250.9164739721391914No Hit
TTAACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA14060.7929614798939709No Hit
TTAACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC12810.7224634820371102No Hit
TTAACTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC10680.6023348936890193No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9930.5600360949749027No Hit
TTAACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA9680.5459364954035305No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8490.47882240144379895No Hit
TTAACTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC6860.38689301223845246No Hit
TTAACTGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5990.3378264057300773No Hit
TTAACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5810.3276746940386893No Hit
TTAACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA5540.31244712650160733No Hit
TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.3028593987930743No Hit
TTAACTGGGGTCAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAG5340.30116744684450963No Hit
TTAACTGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT5090.28706784727313744No Hit
TTAACTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4850.27353223168462015No Hit
TTAACTGGGATGTGTGTGTGTGTAAGTATGTAGGTGTATGTGAGCTTCTGTGTGTT4680.26394450397608704No Hit
TTAACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4420.24928092042186004No Hit
TTAACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4210.23743725678190739No Hit
TTAACTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4150.23405335288477808No Hit
TTAACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4120.23236140093621344No Hit
TTAACTGGGAATGCTTTGATAGCAAGCATAGAATTTCACATTTGACATGCTCTGTG4060.2289774970390841No Hit
TTAACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3840.21656984941627655No Hit
TTAACTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3570.20134228187919465No Hit
TTAACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC3530.19908634594777508No Hit
TTAACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.18949861823924202No Hit
TTAACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3270.18442276239354802No Hit
TTAACTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3020.17032316282217583No Hit
TTAACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.16468332299362698No Hit
TTAACTGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC2780.15678754723365856No Hit
TTAACTGGGAAGTCTAATAGGTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTT2650.14945575545654502No Hit
TTAACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC2600.1466358355422706No Hit
TTAACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA2580.1455078675765608No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2500.14099599571372173No Hit
TTAACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.1381760757994473No Hit
TTAACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA2370.13366420393660822No Hit
TTAACTGGGACTTAATCAATTTCTAACAAACTGTCCTTTTCTAAATTCTGGTTATT2360.13310021995375332No Hit
TTAACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2200.12407647622807512No Hit
TTAACTGGGGGCCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC2200.12407647622807512No Hit
TTAACTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA2120.11956460436523603No Hit
TTAACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2100.11843663639952626No Hit
TTAACTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA2030.11448874851954205No Hit
TTAACTGGGCAGTTCCCACCACCTCCACAGAGCCCCCTAACAATCCCGGATATCAG1960.11054086063955783No Hit
TTAACTGGGATAGTTTCTACTGACAGGTTTCAGAGCTCCCAAAGATTAATTTCCAA1840.1037730528452992No Hit
TTAACTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1840.1037730528452992No Hit
TTAACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1820.10264508487958941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCTT150.00818791550.3535850
AAGCAGG150.00818791550.3535850
TAATCGC150.00818791550.3535850
CTAATCG150.00819715150.33922649
ACGGTAA251.9986264E-550.33922249
ATGCTCT204.042859E-450.32488347
TCATAAT204.042859E-450.32488348
TTATCTC150.008206394550.3248848
AACGGTA252.0020012E-550.3248848
TCTAATC150.008206394550.3248848
TACTCTT150.008206394550.3248847
CTACTCT150.008224905550.2962146
ACTGACG301.0011445E-650.2962146
GTCTCGT204.0599512E-450.2818943
CACCTTC150.00823417250.2818944
TCTCGTG150.00823417250.2818944
ACTACTC204.0599512E-450.2818945
ATGCATG150.00823417250.2818943
GACTACT204.0599512E-450.2818944
TGCAAAG150.00823417250.2818943
Produced by FastQC (version 0.11.5)