##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212652_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176363 Sequences flagged as poor quality 0 Sequence length 58 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.47097747259913 39.0 38.0 39.0 34.0 40.0 2 35.292793839977776 38.0 33.0 39.0 28.0 40.0 3 36.8793170903194 38.0 36.0 39.0 33.0 40.0 4 36.37712558756655 38.0 35.0 39.0 31.0 40.0 5 36.816684905564095 38.0 36.0 39.0 33.0 40.0 6 36.60563723683539 38.0 36.0 39.0 31.0 40.0 7 35.74033669193652 38.0 35.0 39.0 30.0 40.0 8 36.17012071693042 38.0 36.0 39.0 30.0 40.0 9 35.9938819366874 38.0 35.0 39.0 29.0 40.0 10 35.00038556840153 38.0 33.0 39.0 27.0 40.0 11 35.629820313784634 38.0 35.0 39.0 29.0 40.0 12 35.3562028316597 38.0 35.0 39.0 29.0 40.0 13 35.23829828251958 38.0 34.0 39.0 28.0 40.0 14 35.23472610468182 38.0 34.0 39.0 28.0 40.0 15 35.29099074068824 38.0 34.0 39.0 29.0 40.0 16 34.74137999467008 38.0 33.0 39.0 27.0 40.0 17 34.74905734195948 38.0 33.0 39.0 28.0 40.0 18 34.604747027437725 38.0 33.0 39.0 27.0 40.0 19 34.08515391550382 37.0 33.0 39.0 25.0 40.0 20 34.319845999444325 38.0 33.0 39.0 26.0 40.0 21 34.078259045264595 38.0 33.0 39.0 26.0 40.0 22 34.01058612067157 38.0 33.0 39.0 26.0 40.0 23 33.67862306719663 37.0 33.0 39.0 25.0 40.0 24 33.78896934164195 37.0 33.0 39.0 25.0 40.0 25 33.65762092956005 37.0 33.0 39.0 25.0 40.0 26 33.26380816837999 37.0 33.0 39.0 23.0 40.0 27 33.25042100667373 37.0 33.0 39.0 23.0 40.0 28 32.5706752550138 36.0 32.0 39.0 19.0 40.0 29 32.781598181024364 37.0 32.0 39.0 20.0 40.0 30 32.126528807062705 36.0 31.0 38.0 16.0 40.0 31 31.38728644897172 36.0 30.0 38.0 2.0 39.0 32 30.994749465590854 36.0 29.0 38.0 2.0 39.0 33 31.189546560219547 36.0 30.0 38.0 2.0 39.0 34 30.648089451869158 35.0 29.0 38.0 2.0 39.0 35 30.21619047079036 35.0 28.0 38.0 2.0 39.0 36 31.018342849690693 36.0 31.0 39.0 2.0 40.0 37 30.794854929888924 36.0 30.0 39.0 2.0 40.0 38 30.722736628431132 36.0 30.0 39.0 2.0 40.0 39 30.687162273265933 36.0 30.0 39.0 2.0 40.0 40 30.521991574196402 36.0 30.0 39.0 2.0 40.0 41 30.879929463663014 36.0 31.0 39.0 2.0 40.0 42 30.787636862607236 36.0 31.0 39.0 2.0 40.0 43 30.572177837755085 36.0 30.0 39.0 2.0 40.0 44 30.583166537198846 36.0 30.0 39.0 2.0 40.0 45 30.332240889528983 36.0 30.0 39.0 2.0 39.0 46 30.066952818901925 36.0 30.0 39.0 2.0 39.0 47 29.751002194337815 35.0 29.0 39.0 2.0 39.0 48 29.870222212142004 36.0 29.0 39.0 2.0 39.0 49 29.700107165335133 35.0 29.0 39.0 2.0 39.0 50 29.426875251611733 35.0 28.0 39.0 2.0 39.0 51 29.139581431479392 35.0 28.0 39.0 2.0 39.0 52 28.945476091924043 35.0 27.0 39.0 2.0 39.0 53 28.742553710245346 35.0 27.0 39.0 2.0 39.0 54 28.51694516423513 35.0 26.0 39.0 2.0 39.0 55 28.184307366057507 35.0 24.0 39.0 2.0 39.0 56 27.78893532090064 35.0 20.0 39.0 2.0 39.0 57 27.484438345911556 35.0 17.0 39.0 2.0 39.0 58 27.070116747843937 35.0 15.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -2.3804456713968563 1 2 -3.1478667895573125 1 3 -3.614374390440169 1 4 -3.8006229102646785 1 5 -4.251614182846417 1 6 -4.300445518837478 1 7 -4.948522409028975 1 8 -4.033261007002828 1 9 -4.09321508309484 1 10 -3.325995482041211 1 11 -2.656418112534297 1 12 -2.7516860209904976 1 13 -3.3886935817891057 1 14 -3.53195682239204 1 15 -3.2555982245784847 1 16 -3.739030049557446 1 17 -3.5537423515534563 1 18 -3.8868917044843663 1 19 -3.6470759448987806 1 20 -3.2864868192245993 1 21 -3.220815449197609 1 22 -3.0235076310763915 1 23 -3.291879010492444 1 24 -3.7161934599256377 1 25 -2.63127400254184 1 26 -2.744348818320688 1 27 -3.064377628071682 1 28 -2.325965654171913 1 29 -3.5104767102711314 1 30 -2.9541937689376496 1 31 -2.908074799979964 1 32 -2.1763645659578827 1 33 -2.034059991087595 1 34 -1.7158729838266602 1 35 -2.5336942292990443 1 36 -0.7946415200748902 1 37 -0.9573364735474961 1 38 -0.6097255180378021 1 39 -0.6059453663131862 1 40 -0.6072556046836901 1 41 -0.32791910044520733 1 42 -0.29894086192239655 1 43 -0.4707002528626738 1 44 0.1988791134907899 1 45 -0.22771057481339696 1 46 -0.2077519752486907 1 47 -0.2583566443463958 1 48 -0.3373117126480487 1 49 0.12654744880856938 1 50 0.38225311684907126 1 51 0.22682122751716705 1 52 -0.8852367848705747 1 53 0.01168226888186652 1 54 -0.42853063410322534 1 55 -0.9405923842895056 1 56 -0.23810026400087025 1 57 -0.9900481151469087 1 58 -0.5658062605900618 2 1 -0.4460444509544459 2 2 -0.5910399398242845 2 3 -0.29496172835015955 2 4 -0.4104627271983148 2 5 -0.49970724158021085 2 6 -1.0336644356948383 2 7 -1.3137016615079986 2 8 -1.2964189017396706 2 9 -1.126014472873635 2 10 -0.6217620724302293 2 11 -0.6512693711155322 2 12 -0.8552329317456469 2 13 -0.9970078457097742 2 14 -0.7729941984408555 2 15 -1.4451481864396598 2 16 -0.31740533559863593 2 17 -0.1323083317212692 2 18 -0.6429939165362342 2 19 -0.4868928785372262 2 20 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0.8751701444060522 3 42 0.34694014494259307 3 43 0.44119906063847125 3 44 0.7351109975487624 3 45 0.21298355180750406 3 46 -0.18582215068728658 3 47 0.6836723411608503 3 48 -0.41492422218275493 3 49 0.14599824972390252 3 50 -0.6116446710990608 3 51 -0.005634912833709649 3 52 0.7877227879745838 3 53 -0.5987296304316345 3 54 0.8893564749738161 3 55 0.6637173029721275 3 56 1.009611406479678 3 57 1.3032394515960348 3 58 1.6205019011185584 4 1 -1.0891413235707645 4 2 -2.0391711373833985 4 3 -1.1687222165271223 4 4 -1.3571446493951171 4 5 -1.5798750524116372 4 6 -1.2634889970983423 4 7 -1.6028702351159296 4 8 -1.8506523113506574 4 9 -1.636693343964403 4 10 -1.8325172211716492 4 11 -1.0672876777516933 4 12 -1.0929903688165865 4 13 -1.5104327122238885 4 14 -1.6797829093485603 4 15 -1.3469025724045736 4 16 -1.4672909191226609 4 17 -1.2928727863360692 4 18 -1.2760221392669777 4 19 -1.5340324666379104 4 20 -1.3930085583550351 4 21 -2.040380666588913 4 22 -1.9104641528155213 4 23 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-1.4816968794683554 118 29 -1.3697652478205384 118 30 -1.9609131365265817 118 31 -0.6977981201380672 118 32 -0.7467202971831775 118 33 -0.41014694760933423 118 34 -0.5729876083325891 118 35 0.4846852173412728 118 36 -0.09226997857291153 118 37 0.2507662932509227 118 38 -0.3350219607255518 118 39 -0.5132576192776135 118 40 0.242942023774809 118 41 -1.3846384680341401 118 42 -1.0248301899856358 118 43 -1.0363129010840169 118 44 -1.5042829418451795 118 45 -1.2986591914141883 118 46 -1.830479247975962 118 47 -1.140570083081574 118 48 -1.1940310802369787 118 49 -1.099934764630163 118 50 -1.2770354207003365 118 51 -0.8413605906646495 118 52 -0.3253553619456717 118 53 -0.6877248457426397 118 54 -0.19947925070401595 118 55 -1.181896732115593 118 56 -0.5363216078743882 118 57 -1.270285269297105 118 58 -0.5276639680999438 119 1 -0.43854843819527645 119 2 -2.171582204577078 119 3 -1.0137815050646708 119 4 -1.0334292343753546 119 5 -1.555369123557881 119 6 -1.9543980880074372 119 7 -1.6771785355111888 119 8 -0.7878064615482856 119 9 -0.9177210119485935 119 10 -1.2040587231479307 119 11 -0.6842837251825173 119 12 -0.7064291039944521 119 13 -1.3954129493780343 119 14 -1.0536959528268213 119 15 -1.6287207542227549 119 16 -1.0542474408617935 119 17 -1.3972206124230198 119 18 -1.4503304396622312 119 19 -0.9261273282979872 119 20 -1.8123761472878428 119 21 -1.3071790855612448 119 22 -1.4839819393767897 119 23 -1.2527485757098376 119 24 -1.46915788680311 119 25 -1.129692974873855 119 26 -0.3625306365025054 119 27 -1.0420456122614041 119 28 -1.236242334013813 119 29 -0.02431070236599453 119 30 -0.20636768198112776 119 31 -1.0796163019562464 119 32 0.47146152099864125 119 33 -0.34651058397297163 119 34 -0.1457148810598632 119 35 -0.6516784190223639 119 36 0.398639112336177 119 37 0.8962208387054673 119 38 0.874068948365359 119 39 0.8867423807223851 119 40 0.7065783874111702 119 41 0.515361531965862 119 42 0.7751698100143614 119 43 0.018232553461437817 119 44 0.28662614906391326 119 45 1.0467953540403556 119 46 0.5604298429331287 119 47 0.5957935532820642 119 48 0.8423325561266566 119 49 0.46370159900619967 119 50 1.3684191247542117 119 51 1.649548500244439 119 52 1.4928264562361448 119 53 1.0213660633482675 119 54 0.4550662038414366 119 55 1.1090123587934997 119 56 1.245496573943793 119 57 1.3388056397938009 119 58 0.8814269409909663 120 1 -2.528621583440831 120 2 -3.850265590157015 120 3 -3.283164995137817 120 4 -3.4742860786804712 120 5 -3.730499740067806 120 6 -4.015213135029377 120 7 -2.8209611896386733 120 8 -4.3436058345890345 120 9 -4.762130625323724 120 10 -3.1689490052795932 120 11 -2.723259691744694 120 12 -3.1283726776621634 120 13 -2.443166345407292 120 14 -3.076684458573947 120 15 -2.6756382045884806 120 16 -2.8875807741951256 120 17 -2.5167608423080807 120 18 -2.53319355355983 120 19 -2.953922521610423 120 20 -3.661174475396514 120 21 -3.1202407365539315 120 22 -3.1919234231803415 120 23 -3.6332083458247766 120 24 -4.271665723793703 120 25 -3.4166313238811696 120 26 -3.048946519470114 120 27 -2.81487737819662 120 28 -3.2240166286846588 120 29 -3.177393252000268 120 30 -2.9224596360041133 120 31 -2.004276699030438 120 32 -2.5066993985415884 120 33 -2.4434802809426657 120 34 -2.405902968834159 120 35 -2.4443638944664627 120 36 -2.199688996336757 120 37 -2.179850216675934 120 38 -1.400330215688978 120 39 -1.905420628682002 120 40 -2.1012586031844407 120 41 -1.4772194502702938 120 42 -1.058685989358679 120 43 -1.8869921069356366 120 44 -2.8913257840604345 120 45 -2.1478754923546255 120 46 -1.7738439292716777 120 47 -2.626672486425356 120 48 -1.842933901553593 120 49 -2.211533510711668 120 50 -2.0605254938455815 120 51 -1.218581071333407 120 52 -1.0250418823218475 120 53 -0.6398669565054398 120 54 -0.590074861989283 120 55 -0.4841955826903046 120 56 0.4566773471935335 120 57 0.4257439888011163 120 58 -0.11240795974048723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 708.0 3 68.0 4 475.0 5 242.0 6 546.0 7 1052.0 8 552.0 9 1139.0 10 1148.0 11 876.0 12 1172.0 13 1328.0 14 1294.0 15 1486.0 16 1728.0 17 1716.0 18 1851.0 19 1905.0 20 1699.0 21 1384.0 22 1750.0 23 1847.0 24 1809.0 25 1826.0 26 2186.0 27 2619.0 28 3176.0 29 4173.0 30 5176.0 31 6232.0 32 7711.0 33 9319.0 34 11344.0 35 13328.0 36 16425.0 37 23433.0 38 25432.0 39 16184.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 41.114632887850625 43.52273436038171 5.257338557407166 10.105294194360495 11 29.281652047198108 36.94822610184676 16.485317215062118 17.284804635893018 12 51.07307088221452 14.402680834415381 19.443987684491645 15.08026059887845 13 22.489978056621855 15.699438090755999 26.48741516077635 35.323168691845794 14 21.34404608676423 33.89656560616456 27.57097577156206 17.188412535509148 15 37.992095847768525 17.968054523908076 27.6152027352676 16.4246468930558 16 24.539727720667035 20.412444787171914 38.45761299138708 16.59021450077397 17 40.05375277127289 17.673208099204484 20.37105288524237 21.901986244280263 18 36.42997680919467 18.964295231993106 22.711679887504747 21.894048071307473 19 22.677659146192795 21.3655925562618 38.516015263972605 17.4407330335728 20 26.681635962599444 33.08271103021643 21.69867487709868 18.536978130085448 21 24.077140297356454 22.444628417841386 36.90418136248682 16.574049922315343 22 27.32494514031039 22.03743500473466 22.585181363015213 28.052438491939736 23 27.0095715484588 33.87522964911883 22.254417201569552 16.86078160085282 24 25.404988574701044 33.09934623475446 24.39740761951203 17.098257571032473 25 22.42023553693235 21.506211620351205 22.86307218634294 33.210480656373505 26 36.05063319854588 18.60860787060405 26.166724324692193 19.17403460615788 27 22.104403642673258 18.083513841477934 25.436338274153126 34.375744241695685 28 26.455725636764765 35.505696995820124 21.50793155664952 16.53064581076559 29 40.27686409727106 21.496381825193385 22.43154957693442 15.79520450060114 30 23.44007942135867 32.95929655368033 25.59296553680329 18.00765848815771 31 35.559828914377775 22.754501208263843 25.798418479062434 15.887251398295952 32 29.202536096271846 22.70067031882677 35.812151938934065 12.284641645967312 33 35.28120853322446 21.959103126807523 26.02327243033582 16.7364159096322 34 24.230566378005 24.843617968479734 31.53160816872813 19.39420748478713 35 19.463380329616733 38.30688610868505 31.0748273085081 11.154906253190115 36 25.09004690947662 34.01022138777177 25.9942029643159 14.905528738435708 37 22.392403124521365 30.524213906048796 34.20409228343062 12.879290685999218 38 25.432509262989463 31.244503203036782 30.232808856155565 13.09017867781819 39 23.346967952027324 27.792496610256034 29.272637532833713 19.587897904882933 40 20.247246980330306 28.90032395141296 34.752440982406775 16.09998808584996 41 24.126447410971107 27.677958505188272 33.13457730777304 15.061016776067582 42 19.594333840321447 31.100315543347484 31.276815509296043 18.028535107035026 43 17.012184394667695 27.828317509321316 35.190597529070594 19.968900566940395 44 19.0490780141844 25.29985815602837 33.42070921985816 22.23035460992908 45 19.54601414947152 25.7566903625873 37.23228621192437 17.465009276016815 46 20.106897862610147 28.434604494930294 36.79975942306928 14.658738219390274 47 21.43205839946414 27.26235482442696 34.98121090335252 16.324375872756377 48 26.26207585510109 26.279671695671425 32.669232253743374 14.789020195484111 49 26.071923330229602 26.335929870778735 31.938546090432173 15.65360070855949 50 16.416097018145482 29.517634501396678 34.97320191674426 19.093066563713577 51 16.585399081553298 30.023216606969523 35.96417036107784 17.427213950399338 52 18.944841402716218 27.46125163875347 37.53440672867918 16.059500229851135 53 13.475829572227427 28.770615118226463 39.604870986970965 18.14868432257515 54 14.503253352713399 30.814871171774755 36.149120515994234 18.532754959517618 55 18.676700269771406 28.372000567939796 34.154195655260544 18.797103507028254 56 16.05319064335458 30.267657316837642 32.79313361280574 20.886018427002035 57 22.258068180527317 29.721916143176834 30.701684633822175 17.318331042473677 58 21.75829418881385 32.58035749785581 31.122306980125753 14.539041333204587 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 386.0 9 742.5 10 713.0 11 656.0 12 599.0 13 536.0 14 473.0 15 665.5 16 1106.0 17 1354.0 18 1369.0 19 1384.0 20 1599.5 21 1815.0 22 2329.0 23 3552.5 24 4262.0 25 4621.0 26 4980.0 27 5954.0 28 7006.5 29 7085.0 30 6318.5 31 5552.0 32 7501.5 33 9451.0 34 7158.0 35 4346.0 36 3827.0 37 3631.0 38 3435.0 39 3606.5 40 3778.0 41 3988.5 42 4259.0 43 4319.0 44 5109.5 45 5900.0 46 5534.5 47 5559.0 48 5949.0 49 5966.0 50 5983.0 51 7231.5 52 8480.0 53 9584.5 54 10017.0 55 9345.0 56 9452.5 57 9560.0 58 9953.5 59 10235.5 60 10124.0 61 10395.0 62 10666.0 63 7643.0 64 4620.0 65 3543.0 66 1935.0 67 1404.0 68 1118.0 69 832.0 70 714.5 71 597.0 72 446.5 73 236.0 74 176.0 75 149.5 76 123.0 77 98.5 78 54.5 79 35.0 80 25.0 81 15.0 82 12.0 83 9.0 84 7.0 85 4.5 86 4.0 87 3.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0011340247103984397 21 0.005103111196792978 22 0.0022680494207968794 23 0.0039690864863945385 24 0.0 25 0.0 26 0.020412444787171913 27 0.005103111196792978 28 0.02381451891836723 29 0.017577383011175812 30 0.049897087257531336 31 0.04365995135033992 32 0.016443358300777373 33 0.009639210038386737 34 0.018144395366375035 35 0.020979457142371132 36 0.03742281544314851 37 0.04536098841593758 38 0.07030953204470325 39 0.0544331860991251 40 0.05783526023032042 41 0.056701235519921976 42 0.09015496447667595 43 0.08731990270067984 44 0.06407239613751184 45 0.05783526023032042 46 0.06804148262390637 47 0.11170143397424631 48 0.10489728571185566 49 0.13097985405101975 50 0.13097985405101975 51 0.11113442161904707 52 0.09185600154227361 53 0.12360869343342991 54 0.1343819281822151 55 0.1632995582973753 56 0.17974291659815267 57 0.17067071891496516 58 0.17350578069096126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 176363.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.64932553880349 #Duplication Level Percentage of deduplicated Percentage of total 1 75.18038500285893 31.312123291166515 2 11.324093990796962 9.432817541094222 3 4.615133280692678 5.766515652376065 4 2.5008849075611947 4.166406786003867 5 1.396792550439731 2.908773382171997 6 0.8726549949628339 2.180729518096199 7 0.7610205026274948 2.218719345894547 8 0.528221744220873 1.7600063505383783 9 0.4043346856536063 1.5156240254475146 >10 2.1346693168513626 15.354127566439674 >50 0.14703079478312958 4.310994936579668 >100 0.114357284831323 8.790959555008705 >500 0.010891169983935524 2.8696495296632514 >1k 0.009529773735943584 7.4125525195194 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 4019 2.2788226555456643 No Hit GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 2793 1.5836655080714208 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGTGG 1710 0.9695911273906659 No Hit GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1293 0.7331469752725912 No Hit GATCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1104 0.6259816401399386 No Hit GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1077 0.6106723065495597 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 1077 0.6106723065495597 No Hit GATCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 932 0.5284555150456729 No Hit GATCTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 743 0.42129017991302026 No Hit GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 676 0.38330035211467256 No Hit GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 625 0.3543827219995124 No Hit GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 557 0.31582588184596544 No Hit GATCTTGGGAGTGGCACCAGGACAAGTGGCCAGAGGTGCCTCAGCGTGTTGTAGGATG 514 0.291444350572399 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGATCTTGGGAAGCAGTGG 509 0.28860928879640285 No Hit GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 505 0.286341239375606 No Hit GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 459 0.2602586710364419 No Hit GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 453 0.25685659690524654 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 446 0.252887510418852 No Hit GATCTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 438 0.24835141157725826 No Hit GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 435 0.2466503745116606 No Hit GATCTTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA 375 0.2126296331997074 No Hit GATCTTGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 342 0.19391822547813317 No Hit GATCTTGGGAGTATTAAGATACCACGCTGCTCACATCTGCTTCCCTCAAGGGGAACAA 321 0.18201096601894956 No Hit GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 308 0.17463980540135968 No Hit GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 307 0.17407279304616047 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 276 0.15649541003498466 No Hit GATCTTGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 270 0.15309333590378935 No Hit GATCTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG 266 0.15082528648299245 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA 261 0.14799022470699635 No Hit GATCTTGGGGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGTGCTTGTCCAGG 258 0.1462891876413987 No Hit GATCTTGGGATTTGACATTATTTTAGTAAACTTACCAAAATGAGTACATTATTTAAAA 256 0.14515516293100028 No Hit GATCTTGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 249 0.14118607644460573 No Hit GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 233 0.13211387876141822 No Hit GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 232 0.131546866406219 No Hit GATCTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 228 0.12927881698542212 No Hit GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 227 0.1287118046302229 No Hit GATCTTGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 226 0.12814479227502368 No Hit GATCTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC 220 0.12474271814382837 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 217 0.1230416810782307 Illumina Single End Adapter 2 (96% over 25bp) GATCTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 215 0.12190765636783225 No Hit GATCTTGGGATTTGTTGTTCTGATTATTATGTGTCGGGAGGAATTTCTTTTCTGGTCC 213 0.12077363165743382 No Hit GATCTTGGGGAATATTGATGTCAGTATTTCAACTGCTGTAAAGTGATAAACTTTTGTA 212 0.1202066193022346 No Hit GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT 199 0.11283545868464473 No Hit GATCTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 195 0.11056740926384785 No Hit GATCTTGGGAGCTCTAACCTGGCATGAGAAGCAAACAGGCTGCTGGCCTCCCCTCCAT 185 0.10489728571185566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.006804148262390637 0.0 0.0 0.0 0.0 11 0.015876345945578154 0.0 0.0 0.0 0.0 12 0.016443358300777373 0.0 0.0 0.0 0.0 13 0.017577383011175812 0.0 0.0 0.0 0.0 14 0.047629037836734466 0.0 0.0 0.0 0.0 15 0.05046409961273056 0.0 0.0 0.0 0.0 16 0.052732149033527444 0.0 0.0 0.0 0.0 17 0.05613422316472276 0.0 0.0 0.0 0.0 18 0.0572682478751212 0.0 0.0 0.0 0.0 19 0.0601033096511173 0.0 0.0 0.0 0.0 20 0.09866014980466424 0.0 0.0 0.0 0.0 21 0.10943338455344942 0.0 0.0 0.0 0.0 22 0.12247466872303148 0.0 0.0 0.0 0.0 23 0.1434541258654026 0.0 0.0 0.0 0.0 24 0.1638665706525745 0.0 0.0 0.0 0.0 25 0.17123773127016437 0.0 0.0 0.0 0.0 26 0.17577383011175812 0.0 0.0 0.0 0.0 27 0.17917590424295346 0.0 0.0 0.0 0.0 28 0.2494854362876567 0.0 0.0 0.0 0.0 29 0.257990621615645 0.0 0.0 0.0 0.0 30 0.29881551118998884 0.0 0.0 0.0 0.0 31 0.3016505729659849 0.0 0.0 0.0 0.0 32 0.30902173358357476 0.0 0.0 0.0 0.0 33 0.31922795597716075 0.0 0.0 0.0 0.0 34 0.32603210423955137 0.0 0.0 0.0 0.0 35 0.4524758594489774 0.0 0.0 0.0 0.0 36 0.4604140324217665 0.0 0.0 0.0 0.0 37 0.469486230104954 0.0 0.0 0.0 0.0 38 0.47912544014334074 0.0 0.0 0.0 0.0 39 0.48933166253692667 0.0 0.0 0.0 0.0 40 0.502939959061708 0.0 0.0 0.0 0.0 41 0.5188163050072861 0.0 0.0 0.0 0.0 42 0.5307235644664697 0.0 0.0 0.0 0.0 43 0.5431978362808526 0.0 0.0 0.0 0.0 44 0.5568061328056338 0.0 0.0 0.0 0.0 45 0.5715484540408136 0.0 0.0 0.0 0.0 46 0.5874247999863917 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTGTCC 15 0.0071716793 52.07019 49 GTCATGT 15 0.0071716793 52.07019 49 GACGTCT 15 0.0071716793 52.07019 52 TTGTAGG 45 9.094947E-11 52.07019 49 TGACGTC 15 0.0071716793 52.07019 51 TAGTTAC 15 0.0071716793 52.07019 52 TCATGTT 15 0.0071716793 52.07019 50 TCTCCCG 20 3.416811E-4 52.07019 51 CCGACGG 15 0.0071716793 52.07019 50 CACCGCA 15 0.0071716793 52.07019 50 GATTCGG 15 0.0071716793 52.07019 49 CCTGCCC 15 0.0071716793 52.07019 51 GATTAAG 15 0.0071716793 52.07019 50 CCCGACG 15 0.0071716793 52.07019 49 AGACTAC 25 1.6363128E-5 52.070187 50 ACTACCC 25 1.6363128E-5 52.070187 52 CAAATAC 25 1.6363128E-5 52.070187 51 TGTTGTA 45 9.094947E-11 52.055397 47 TCAGACT 25 1.6390684E-5 52.055397 48 AAGATTA 15 0.007179744 52.055397 48 >>END_MODULE