FastQCFastQC Report
Tue 31 May 2016
SRR212651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179920
Sequences flagged as poor quality0
Sequence length58
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT43242.4032903512672297No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT27801.545131169408626No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGTGG13980.7770120053357047No Hit
GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG12550.6975322365495776No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA11640.6469542018674967No Hit
GATCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC11230.6241662961316141No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG10210.5674744330813695No Hit
GATCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC9190.510782570031125No Hit
GATCTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT7410.41184971098265893No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA6830.3796131614050689No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6330.3518230324588706No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5750.31958648288128055No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGATCTTGGGAAGCAGTGG5260.29235215651400626No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5250.29179635393508224No Hit
GATCTTGGGAGTGGCACCAGGACAAGTGGCCAGAGGTGCCTCAGCGTGTTGTAGGATG5040.28012449977767895No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4950.27512227656736327No Hit
GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4780.26567363272565586No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4690.2606714095153402No Hit
GATCTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4660.25900400177856825No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.2234326367274344No Hit
GATCTTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA3630.20175633614939975No Hit
GATCTTGGGAGTATTAAGATACCACGCTGCTCACATCTGCTTCCCTCAAGGGGAACAA3370.1873054690973766No Hit
GATCTTGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3180.17674522009782123No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.17007558915073367No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2970.16507336594041797No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2880.16007114273010226No Hit
GATCTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG2870.1595153401511783No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC2850.15840373499333035Illumina Single End Adapter 2 (96% over 25bp)
GATCTTGGGGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGTGCTTGTCCAGG2700.15006669630947087No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2640.1467318808359271No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA2470.13728323699421965No Hit
GATCTTGGGATTTGACATTATTTTAGTAAACTTACCAAAATGAGTACATTATTTAAAA2460.1367274344152957No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.1350600266785238No Hit
GATCTTGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2370.13172521120498No Hit
GATCTTGGGATTTGTTGTTCTGATTATTATGTGTCGGGAGGAATTTCTTTTCTGGTCC2320.12894619831036017No Hit
GATCTTGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA2270.12616718541574032No Hit
GATCTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2240.12449977767896843No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.12116496220542464No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2180.12116496220542464No Hit
GATCTTGGGAGCTCTAACCTGGCATGAGAAGCAAACAGGCTGCTGGCCTCCCCTCCAT2030.11282792352156515No Hit
GATCTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2000.11116051578479323No Hit
GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.11004891062694531No Hit
GATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.10671409515340151No Hit
GATCTTGGGGAATATTGATGTCAGTATTTCAACTGCTGTAAAGTGATAAACTTTTGTA1880.10449088483770565No Hit
GATCTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1870.10393508225878167No Hit
GATCTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1840.10226767452200979No Hit
GATCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAACATCGAACT1800.10004446420631392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG307.80572E-752.14489427
GAATATA150.007131347452.14489427
GTATTTC353.7916834E-852.11579524
ACGCGAT251.6279795E-552.1157924
ACGCGAA307.835861E-752.1157924
TCGTTAG150.00714711152.1157924
GTCCTCG150.00714711152.1157925
TCCTCGC150.00714711152.1157926
CGCGATC251.6279795E-552.1157925
CGTTAGT150.00714711152.1157925
AAACCGC307.835861E-752.1157926
TACTCAC150.00714711152.1157924
CCGAACA150.00714711152.1157924
CTGGGTA251.6279795E-552.1157925
TAGTAAA307.835861E-752.1157924
GCCGAAC150.007170805252.0722123
TTAGTAA307.881263E-752.0722123
ACCACGC251.6360662E-552.07220521
ATTACTT203.4163377E-452.072222
TATGGGT150.007178716452.05769752
Produced by FastQC (version 0.11.5)