FastQCFastQC Report
Tue 31 May 2016
SRR212650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186208
Sequences flagged as poor quality0
Sequence length58
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT40962.199690668499742No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT27601.4822134387351777No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGTGG24501.3157329438047773No Hit
GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG11320.6079223234232686No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA10410.5590522426533769No Hit
GATCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10190.5472374978518646No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG10180.5467004639972504No Hit
GATCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC8080.43392335452826947No Hit
GATCTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT6230.3345720914246434No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5910.31738700807698916No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5320.2857020106547517No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5040.27066506272555424No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGATCTTGGGAAGCAGTGG4880.2620725210517271No Hit
GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4840.2599243856332703No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.24327633614023028No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4400.23629489603024575No Hit
GATCTTGGGAGTGGCACCAGGACAAGTGGCCAGAGGTGCCTCAGCGTGTTGTAGGATG4370.23468379446640314No Hit
GATCTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4160.22340608351950506No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4010.21535057570029212No Hit
GATCTTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA3500.18796184911496822No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.18151744285959787No Hit
GATCTTGGGAGTATTAAGATACCACGCTGCTCACATCTGCTTCCCTCAAGGGGAACAA3350.17990634129575528No Hit
GATCTTGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3240.17399896889499913No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.16486939336655784No Hit
GATCTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3010.16164719023887267No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2740.14714727616428938No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2570.1380177006358481No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA2440.13103626052586356No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.13103626052586356No Hit
GATCTTGGGATTTGACATTATTTTAGTAAACTTACCAAAATGAGTACATTATTTAAAA2440.13103626052586356No Hit
GATCTTGGGGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGTGCTTGTCCAGG2310.12405482041587901No Hit
GATCTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2280.12244371885203643No Hit
GATCTTGGGATTTGTTGTTCTGATTATTATGTGTCGGGAGGAATTTCTTTTCTGGTCC2200.11814744801512288No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.11814744801512288No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2200.11814744801512288No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC2190.11761041416050867Illumina Single End Adapter 2 (96% over 25bp)
GATCTTGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA2090.11224007561436673No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.10901787248668157No Hit
GATCTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1920.10311050008592541No Hit
GATCTTGGGGAATATTGATGTCAGTATTTCAACTGCTGTAAAGTGATAAACTTTTGTA1910.10257346623131122No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGGGG1900.10203643237669702No Hit
GATCTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1890.10149939852208284No Hit
GATCTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1890.10149939852208284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGT150.007044135652.308059
GGACTAC150.007044135652.308058
AATGGTC150.007044135652.3080510
AAGCGAT203.341228E-452.3080510
GATGGAC150.007044135652.308059
GGGGTAC550.052.308057
ACTGTGT150.007044135652.3080510
GACTGTG203.341228E-452.308059
AGCTATA203.341228E-452.3080510
GACCTAC203.341228E-452.308059
GAGATAA203.341228E-452.308059
GAGGCAT150.007044135652.308059
CCAATGA150.007127545352.15262238
ACGGCGT150.007127545352.15262239
CAATGAT150.007127545352.15262239
CGGTCCT150.007127545352.15262238
TCGGTCA150.007127545352.15262239
TGACGGA203.390698E-452.1526238
CTGACGG251.6396378E-552.0541937
GCTGACG251.6396378E-552.0541936
Produced by FastQC (version 0.11.5)