##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212650_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 186208 Sequences flagged as poor quality 0 Sequence length 58 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.30992223749785 38.0 37.0 39.0 33.0 40.0 2 35.11587579481011 38.0 33.0 39.0 28.0 40.0 3 36.66410143495446 38.0 36.0 39.0 32.0 40.0 4 36.168258076989176 38.0 35.0 39.0 30.0 40.0 5 36.608776207252106 38.0 35.0 39.0 32.0 40.0 6 36.28020815432205 38.0 35.0 39.0 31.0 40.0 7 35.283237025262075 38.0 33.0 39.0 28.0 40.0 8 35.908537764220654 38.0 35.0 39.0 29.0 40.0 9 35.71263855473449 38.0 35.0 39.0 28.0 40.0 10 34.0572155868706 38.0 33.0 39.0 25.0 40.0 11 35.040105688262585 38.0 34.0 39.0 28.0 40.0 12 34.95531341295755 38.0 33.0 39.0 28.0 40.0 13 34.84840608351951 38.0 33.0 39.0 27.0 40.0 14 34.65547130091081 38.0 33.0 39.0 26.0 40.0 15 34.95583970613507 38.0 33.0 39.0 28.0 40.0 16 34.42526099845334 38.0 33.0 39.0 26.0 40.0 17 34.18717240934868 38.0 33.0 39.0 25.0 40.0 18 34.26102530503523 38.0 33.0 39.0 26.0 40.0 19 33.75548848599416 37.0 33.0 39.0 24.0 40.0 20 33.97697735865269 38.0 33.0 39.0 25.0 40.0 21 33.782297216016495 38.0 33.0 39.0 24.0 40.0 22 33.76895729506788 38.0 33.0 39.0 25.0 40.0 23 33.3966478346795 37.0 33.0 39.0 23.0 40.0 24 33.52597095720914 37.0 33.0 39.0 24.0 40.0 25 33.38033274617632 37.0 33.0 39.0 23.0 40.0 26 32.866079867674856 37.0 33.0 39.0 20.0 40.0 27 32.856649553187836 37.0 33.0 39.0 20.0 40.0 28 32.22576366214126 36.0 31.0 39.0 17.0 40.0 29 32.21308429283382 36.0 31.0 39.0 15.0 40.0 30 31.63613271180615 36.0 31.0 38.0 9.0 40.0 31 30.91652345763877 36.0 30.0 38.0 2.0 39.0 32 30.52363485994157 36.0 29.0 38.0 2.0 39.0 33 30.68400927994501 36.0 29.0 38.0 2.0 39.0 34 30.219007776250216 35.0 28.0 38.0 2.0 39.0 35 29.6100328664719 35.0 27.0 38.0 2.0 39.0 36 30.430926705619523 36.0 30.0 39.0 2.0 40.0 37 30.247100017185083 36.0 29.0 39.0 2.0 40.0 38 30.19273608008249 36.0 29.0 39.0 2.0 40.0 39 29.749215930572262 36.0 28.0 39.0 2.0 40.0 40 30.008076989173396 36.0 29.0 39.0 2.0 40.0 41 30.25700292146417 36.0 30.0 39.0 2.0 40.0 42 30.156373517786562 36.0 29.0 39.0 2.0 40.0 43 29.9585893194707 36.0 29.0 39.0 2.0 40.0 44 29.686603153462794 36.0 28.0 39.0 2.0 40.0 45 29.62719646846537 36.0 28.0 39.0 2.0 39.0 46 29.25527367245231 36.0 27.0 39.0 2.0 39.0 47 29.11113378587386 35.0 27.0 39.0 2.0 39.0 48 29.233604356418628 35.0 27.0 39.0 2.0 39.0 49 29.149558558171506 35.0 27.0 39.0 2.0 39.0 50 28.62673998968895 35.0 25.0 39.0 2.0 39.0 51 28.640402130950335 35.0 26.0 39.0 2.0 39.0 52 28.44774660594604 35.0 25.0 39.0 2.0 39.0 53 28.276620768173224 35.0 24.0 39.0 2.0 39.0 54 28.045996949647705 35.0 23.0 39.0 2.0 39.0 55 27.771927092283896 35.0 22.0 39.0 2.0 39.0 56 27.347906642034715 35.0 2.0 39.0 2.0 39.0 57 27.179906341295755 35.0 2.0 39.0 2.0 39.0 58 26.794063627771095 35.0 2.0 39.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.9648716007551243 1 2 -6.750079415567612 1 3 -5.05872918854212 1 4 -5.081528047471579 1 5 -4.481208972739886 1 6 -3.8308267725667235 1 7 -3.218158322731707 1 8 -3.495560051728738 1 9 -3.60800083636364 1 10 -4.825352590978188 1 11 -4.42293180087885 1 12 -4.35613577959154 1 13 -5.259853510181419 1 14 -3.906983570945446 1 15 -4.229292137861176 1 16 -2.474374168318871 1 17 -2.4053983132658736 1 18 -2.1314070515677983 1 19 -2.552899202078393 1 20 -3.8190001219055105 1 21 -4.271030874255487 1 22 -3.039637249562741 1 23 -4.009840572370557 1 24 -3.661093175313802 1 25 -4.28911475138948 1 26 -3.6659733455451864 1 27 -3.567351256924262 1 28 -3.6092399931418306 1 29 -4.698639651861779 1 30 -4.167138228113604 1 31 -2.154771152466111 1 32 -2.9941231601867493 1 33 -1.8579303412212944 1 34 -3.0641120832129687 1 35 -2.889000795763611 1 36 0.15110261424923266 1 37 -0.14378183538471845 1 38 -0.8082854384619438 1 39 0.35822143673949114 1 40 0.2163113190450865 1 41 0.5055650229044168 1 42 0.19275298546485686 1 43 0.7999436690856498 1 44 0.6637456040178762 1 45 -0.3515121191057915 1 46 1.341710796606563 1 47 -0.05291699510837944 1 48 0.8011879893429779 1 49 1.5804808815944007 1 50 0.5905312370086371 1 51 0.4308341490486072 1 52 0.22131918579436416 1 53 0.472374871979202 1 54 0.3588937538159449 1 55 1.5326523982974756 1 56 1.2579306210060857 1 57 -0.026025313074544698 1 58 0.9701159713891165 2 1 -0.4093160451995672 2 2 -1.8453175108057067 2 3 -0.5111101409230727 2 4 -0.09740106334459853 2 5 -0.5288280203589366 2 6 -1.2197156614556093 2 7 -1.7022853068586912 2 8 -0.7177822739509594 2 9 -0.6794294077922132 2 10 -0.46820973383533016 2 11 -0.32769370564075473 2 12 -0.20534212879788782 2 13 -0.5217582720861813 2 14 0.06127039730851891 2 15 -0.06262547119450801 2 16 0.1843559904112908 2 17 0.6739667660992055 2 18 -0.028232448393197274 2 19 0.21694206776287928 2 20 0.3317935288881415 2 21 -0.4853165885412025 2 22 -0.6190023289278201 2 23 -0.8114278739578538 2 24 -1.63728365150428 2 25 -0.558956021230749 2 26 0.024502844931006962 2 27 0.3136011240281178 2 28 -0.5060653899672296 2 29 -0.2780047312268614 2 30 -0.1274556884310627 2 31 -0.3928663905613483 2 32 0.4661943001307094 2 33 0.26111727782632244 2 34 -0.7149057340066172 2 35 0.5316341248713101 2 36 0.8653883285349444 2 37 0.20542451382162952 2 38 0.5964764662999613 2 39 0.48520556372361767 2 40 0.49408909682286506 2 41 0.910326927666322 2 42 0.9070386997505722 2 43 1.3396262087681876 2 44 1.6954916357639078 2 45 1.3389640713703983 2 46 1.8575838124795787 2 47 1.7089877667963833 2 48 1.856743544898535 2 49 1.723338024451543 2 50 2.1302137766911784 2 51 2.0657547839692434 2 52 1.1816366461118228 2 53 1.170787570391898 2 54 1.6366715315937235 2 55 1.4850333506784281 2 56 0.9801528432283071 2 57 1.005720718671487 2 58 0.04154454281768594 3 1 -0.7150804562271347 3 2 -1.90880957429777 3 3 -1.0620708760943316 3 4 -1.2511186907464307 3 5 -1.105269123115825 3 6 -1.1324140741540276 3 7 -1.5849085315871818 3 8 -1.0043319815532996 3 9 -0.6564553058707432 3 10 -0.4728045542196213 3 11 -0.5983703973700756 3 12 -1.2308224963835173 3 13 -1.2899286981513427 3 14 -1.2921130613380925 3 15 -1.5526004085378702 3 16 -1.393338245177688 3 17 -1.195707419364453 3 18 -1.8707554224951224 3 19 -1.43009218453453 3 20 -1.177396111880448 3 21 -0.9577476662354343 3 22 -0.5141568819771081 3 23 -0.8719959608417369 3 24 -0.4196562496663532 3 25 -0.5338933645891473 3 26 0.24797819998530457 3 27 -0.2515617832400494 3 28 0.8853297645858191 3 29 0.1300988610872622 3 30 0.35750671758397345 3 31 1.1710433838747392 3 32 1.4636880344665464 3 33 1.5255283053952482 3 34 0.6468319435205316 3 35 1.3424110672272 3 36 1.8795905006318563 3 37 2.385875641641178 3 38 1.5492751296249416 3 39 1.4676617040744944 3 40 1.8445485788612928 3 41 1.3309618483012429 3 42 1.7249167281549163 3 43 1.3162343959026899 3 44 2.073937751052128 3 45 1.3352046728741591 3 46 1.236447638711823 3 47 0.9362225203469272 3 48 0.8813884905961125 3 49 1.254666345253547 3 50 1.4627116881364586 3 51 0.6104498549801036 3 52 1.9706926193783225 3 53 1.7990248302081078 3 54 1.6262287579930543 3 55 1.2778487224411705 3 56 1.3773959509977303 3 57 1.823598747075831 3 58 1.5908344342128409 4 1 -0.9893316580020652 4 2 -2.2448751480682603 4 3 -1.616366451097413 4 4 -1.370495000372955 4 5 -1.44022536618251 4 6 -1.3584093903132768 4 7 -2.3714240005875524 4 8 -1.3811962734305396 4 9 -1.6187996914247904 4 10 -1.537946918326611 4 11 -1.9810894901840754 4 12 -2.27494920660169 4 13 -2.0408839551462847 4 14 -1.9773712888741457 4 15 -1.8499010371586024 4 16 -1.7075253522897285 4 17 -1.4880160598180439 4 18 -1.3593539680393079 4 19 -1.6727274612509149 4 20 -1.963418545012459 4 21 -1.5259098750361275 4 22 -1.400943520705077 4 23 -1.1039077074212997 4 24 -1.7482646559279047 4 25 -1.1717585192791518 4 26 -0.8231422240255384 4 27 -1.1891311164090368 4 28 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0.25486896934414816 118 53 -0.00309410348977579 118 54 0.14713329205548575 118 55 0.4078327734778533 118 56 0.9292870423625068 118 57 0.8776543406051083 118 58 0.35251135378450016 119 1 -0.1563398547233774 119 2 -0.36974707429777 119 3 -0.13673018060561049 119 4 -0.3617859839795159 119 5 -0.3718339727398856 119 6 -0.7239319312968817 119 7 -1.121680148128533 119 8 -0.7961552898239788 119 9 -0.6380107569985611 119 10 0.23689939314879638 119 11 -0.3600597770693241 119 12 -0.6213887557820144 119 13 -0.6806124387528456 119 14 0.04452932587994951 119 15 -0.7616582092897488 119 16 -1.5898255572077602 119 17 -1.2918070434246047 119 18 -1.1321510991868493 119 19 -1.757512297316488 119 20 -1.2423632171436054 119 21 -1.6690963504459617 119 22 -1.3713584797214722 119 23 -1.2267304533229364 119 24 -1.6820505165836437 119 25 -1.1262923307545591 119 26 -1.169197551894392 119 27 -1.0015030426385465 119 28 -1.6108520963164352 119 29 -0.8242596915443201 119 30 -0.9633931884310627 119 31 -0.40960746198992126 119 32 -0.08142474748833806 119 33 -0.30101069836415206 119 34 -0.2938987895621743 119 35 -0.30690754179535773 119 36 0.5872385269476439 119 37 0.6362280852502025 119 38 -0.29030428766829175 119 39 0.8337918732474279 119 40 0.049024612695880876 119 41 -0.021716723127330084 119 42 0.41435516800454053 119 43 -0.055711092819112906 119 44 0.16610175481152822 119 45 -0.36304485720102875 119 46 -0.23753027482201006 119 47 0.1648409413995573 119 48 0.2535689417239304 119 49 -0.509548880310362 119 50 0.6329419512943524 119 51 0.08460399031844901 119 52 0.3891019238896014 119 53 -0.10252592167159236 119 54 1.2027677617524546 119 55 0.5808914855990643 119 56 0.9652718908473545 119 57 0.7898229012111706 119 58 0.5651060507541956 120 1 -0.7230415353956445 120 2 -2.992843187743148 120 3 -1.9037338570761975 120 4 -1.5138343033072488 120 5 -1.8509568718995482 120 6 -1.4937586119691488 120 7 -1.6485971649352535 120 8 -2.0445796595718804 120 9 -1.847963488090997 120 10 -1.2866037581117098 120 11 -1.1092063106827723 120 12 -2.1348472641853746 120 13 -1.6632148547192287 120 14 -1.4765447287418993 120 15 -2.0192081042477312 120 16 -1.9683050740144807 120 17 -1.227994018214524 120 18 -2.1286059311196226 120 19 -1.6649440200055636 120 20 -1.9646583852108321 120 21 -1.6211709302778914 120 22 -1.500888416696263 120 23 -1.382389591978395 120 24 -0.7796739409533942 120 25 -1.645790755124306 120 26 -1.2756838964322057 120 27 -1.306847055243587 120 28 -0.9182969492576127 120 29 -0.5324398386031426 120 30 -0.7787161926327428 120 31 -0.12395882753613918 120 32 -0.9007524785807739 120 33 -0.05961101349020126 120 34 0.3177740045554742 120 35 -0.14763758381216618 120 36 1.3957338000568882 120 37 1.2474413205443184 120 38 1.9097351030880105 120 39 1.9081747513986898 120 40 2.220046146309329 120 41 1.851970251662589 120 42 1.7324355251473982 120 43 1.5170305038195409 120 44 1.894371187584639 120 45 1.4692161722107357 120 46 1.9551561747578212 120 47 2.4466161514835925 120 48 1.3922244039088056 120 49 1.3395845230509842 120 50 1.5401110689414104 120 51 2.112440124772231 120 52 1.6779018188475838 120 53 1.9056148346309278 120 54 1.5054857239373298 120 55 1.2291976830780555 120 56 1.8386962605952526 120 57 1.8731343507910019 120 58 1.9066239078970533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 902.0 3 79.0 4 651.0 5 281.0 6 739.0 7 1290.0 8 680.0 9 1475.0 10 1398.0 11 1035.0 12 1435.0 13 1590.0 14 1596.0 15 1844.0 16 1989.0 17 1978.0 18 2194.0 19 2184.0 20 1941.0 21 1610.0 22 1902.0 23 1886.0 24 1968.0 25 2002.0 26 2326.0 27 2861.0 28 3409.0 29 4476.0 30 5346.0 31 6552.0 32 8142.0 33 9938.0 34 11804.0 35 14124.0 36 17270.0 37 23775.0 38 25457.0 39 16062.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 40.06268336779655 44.83809503188299 5.380051748963397 9.719169851357057 11 28.540337791725555 38.299004250608576 16.182405382312954 16.97825257535292 12 52.390874720742396 13.816807011514006 18.975554218937962 14.816764048805636 13 22.073702526207253 15.273242825227703 25.765273242825227 36.887781405739815 14 20.788580512115484 35.37710517271009 26.779193160336828 17.0551211548376 15 39.38445179584121 17.511599931259667 26.959636535487196 16.144311737411925 16 24.113357106031962 19.83427135246606 39.84415277539096 16.208218766111017 17 41.214963815418336 17.355704914333607 19.93348699020572 21.495844280042338 18 37.88021996906685 18.38803918199003 22.182183364839318 21.549557484103797 19 22.140831758034025 20.84067279601306 39.920411582746176 17.098083863206735 20 26.06507811199364 34.425129023076465 21.148506769348092 18.3612860955818 21 23.470116590675968 21.96533964888538 38.20533062666817 16.35921313377048 22 26.816587624270223 21.414491881817742 22.055782627143678 29.713137866768356 23 26.419522359524617 35.25055449043269 21.724747189955266 16.60517596008743 24 24.9242782264994 34.52859168241966 24.059653720570545 16.487476370510397 25 22.076729309779953 20.79951876339379 22.31681051845725 34.806941408369006 26 37.36421364793863 17.943138961415723 25.87220234449709 18.820445046148556 27 21.91738875846768 17.44140446631462 24.90746659933709 35.73374017588061 28 25.724295048143052 36.894450653370015 21.296853507565334 16.084400790921595 29 41.34621072386924 21.074000182836 22.137675509117603 15.442113584177159 30 22.747512772250605 34.22855606345792 25.39983866630815 17.62409249798333 31 36.9483951359945 22.00027929360203 25.63217032612899 15.419155244274481 32 28.504133252405023 21.783502441277736 37.569357533047224 12.143006773270024 33 36.25293419349294 21.19817153416019 25.75268441775395 16.79620985459292 34 23.93934184939675 24.13594903254225 31.306739436392743 20.61796968166826 35 19.017099756638714 39.262824817479036 30.575955045314622 11.144120380567628 36 26.160228713914147 33.054964424668434 25.936136368521744 14.848670492895682 37 21.996646819852547 29.76968381230789 35.659781183500634 12.57388818433893 38 26.111959068727565 30.36094331104757 30.653846980673734 12.87325063955113 39 23.001883239171374 27.10411622276029 29.367769706752757 20.52623083131558 40 19.74795109498858 28.270858524788387 36.316001612253125 15.665188767969903 41 24.982532328628707 27.089402229412336 33.457846477979984 14.470218963978972 42 19.315720160184913 31.314000053753325 31.782729056360363 17.5875507297014 43 16.64874629048213 27.12087652143994 36.905401918197065 19.324975269880866 44 18.55996382954674 24.844041832851598 33.5707019328586 23.025292404743066 45 19.031822751735223 25.152285764977233 38.68139076016538 17.134500723122166 46 19.578110801030256 27.59325278400628 38.3738499674684 14.454786447495065 47 21.918088149884916 26.53584718965777 35.56056271376025 15.985501946697067 48 26.880159178295827 25.500255438143636 33.14726681186308 14.472318571697453 49 26.926945231782025 25.68830037272292 32.242952955160305 15.141801440334751 50 16.123445634869412 29.66632244739899 35.28193709392883 18.928294823802762 51 16.355293522757226 30.401781631763825 36.236733244753815 17.00619160072513 52 19.647267073059187 26.918751983988205 37.93008829083788 15.503892652114729 53 13.339537185459319 28.12873172463503 39.70608802297936 18.825643066926297 54 14.16659942440625 31.00944081336238 36.60238306570914 18.22157669652223 55 19.213529699800368 27.880522806885384 34.61093503656312 18.295012456751127 56 15.872503095891885 29.742637161470952 34.01819846013029 20.366661282506865 57 22.757778148528864 28.92432551706322 31.24606450710138 17.071831827306536 58 22.08661947188501 31.92484724502705 31.65567548652795 14.332857796559987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 314.0 9 662.5 10 697.0 11 656.0 12 615.0 13 560.0 14 505.0 15 678.0 16 1102.0 17 1353.0 18 1398.0 19 1443.0 20 1678.0 21 1913.0 22 2461.5 23 3668.5 24 4327.0 25 4829.5 26 5332.0 27 6291.0 28 7327.0 29 7404.0 30 6712.5 31 6021.0 32 8011.0 33 10001.0 34 7710.5 35 4720.0 36 4020.0 37 3870.5 38 3721.0 39 3809.0 40 3897.0 41 4143.0 42 4464.5 43 4540.0 44 5276.0 45 6012.0 46 5754.0 47 5896.5 48 6297.0 49 6426.5 50 6556.0 51 8003.0 52 9450.0 53 11052.0 54 11477.5 55 10301.0 56 10199.0 57 10097.0 58 10416.5 59 10571.0 60 10406.0 61 10645.0 62 10884.0 63 7655.5 64 4427.0 65 3344.5 66 1800.0 67 1338.0 68 1044.5 69 751.0 70 666.0 71 581.0 72 430.5 73 204.5 74 129.0 75 123.5 76 118.0 77 80.0 78 36.5 79 31.0 80 23.5 81 16.0 82 10.0 83 4.0 84 3.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.7894397662828665 11 0.0628329609898608 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.042425674514521394 18 0.0 19 0.0 20 5.370338546141949E-4 21 5.370338546141949E-4 22 0.011277710946898093 23 5.370338546141949E-4 24 0.0 25 0.011277710946898093 26 0.03759236982299364 27 0.0316849974222375 28 0.05155525004296271 29 0.13372142979893453 30 0.1385547344904623 31 0.012888812510740677 32 0.01879618491149682 33 0.02201838803918199 34 0.026851692730709743 35 0.03490720054992267 36 0.06659219797216016 37 0.062295927135246607 38 0.07518473964598728 39 0.19225811995188177 40 0.07142550266368793 41 0.08055507819212923 42 0.09290685684825571 43 0.10740677092283897 44 0.22609125279257603 45 0.11224007561436673 46 0.1262029558343358 47 0.13533253136277712 48 0.1358695652173913 49 0.14983244543736038 50 0.15036947929197456 51 0.1670175287850146 52 0.18527667984189722 53 0.16218422409348685 54 0.16809159649424302 55 0.19601735693418115 56 0.25670218250558513 57 0.21427650799106376 58 0.24327633614023028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 186208.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.97430830039526 #Duplication Level Percentage of deduplicated Percentage of total 1 79.88738637890118 38.32542103454202 2 9.481484798280572 9.09735349716446 3 3.7881162405409037 5.451967692043307 4 1.9500291049120135 3.74205189895171 5 1.10486723682443 2.650262072521052 6 0.7332198988044597 2.110543048633786 7 0.530604934402006 1.7818783296098988 8 0.3917968924909327 1.5036947929197457 9 0.3167957730712399 1.3678252277023544 >10 1.6030090001343305 13.378587386148824 >50 0.11530022836161732 3.842477229764564 >100 0.08395647696234272 7.8471386836226165 >500 0.005597098464156181 1.642249527410208 >1k 0.007835937849818654 7.258549578965458 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 4096 2.199690668499742 No Hit GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 2760 1.4822134387351777 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGTGG 2450 1.3157329438047773 No Hit GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1132 0.6079223234232686 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 1041 0.5590522426533769 No Hit GATCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1019 0.5472374978518646 No Hit GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1018 0.5467004639972504 No Hit GATCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 808 0.43392335452826947 No Hit GATCTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 623 0.3345720914246434 No Hit GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 591 0.31738700807698916 No Hit GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 532 0.2857020106547517 No Hit GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 504 0.27066506272555424 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGATCTTGGGAAGCAGTGG 488 0.2620725210517271 No Hit GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 484 0.2599243856332703 No Hit GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 453 0.24327633614023028 No Hit GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 440 0.23629489603024575 No Hit GATCTTGGGAGTGGCACCAGGACAAGTGGCCAGAGGTGCCTCAGCGTGTTGTAGGATG 437 0.23468379446640314 No Hit GATCTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 416 0.22340608351950506 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 401 0.21535057570029212 No Hit GATCTTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA 350 0.18796184911496822 No Hit GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 338 0.18151744285959787 No Hit GATCTTGGGAGTATTAAGATACCACGCTGCTCACATCTGCTTCCCTCAAGGGGAACAA 335 0.17990634129575528 No Hit GATCTTGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 324 0.17399896889499913 No Hit GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 307 0.16486939336655784 No Hit GATCTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG 301 0.16164719023887267 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 274 0.14714727616428938 No Hit GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 257 0.1380177006358481 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA 244 0.13103626052586356 No Hit GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 244 0.13103626052586356 No Hit GATCTTGGGATTTGACATTATTTTAGTAAACTTACCAAAATGAGTACATTATTTAAAA 244 0.13103626052586356 No Hit GATCTTGGGGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGTGCTTGTCCAGG 231 0.12405482041587901 No Hit GATCTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 228 0.12244371885203643 No Hit GATCTTGGGATTTGTTGTTCTGATTATTATGTGTCGGGAGGAATTTCTTTTCTGGTCC 220 0.11814744801512288 No Hit GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 220 0.11814744801512288 No Hit GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 220 0.11814744801512288 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 219 0.11761041416050867 Illumina Single End Adapter 2 (96% over 25bp) GATCTTGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 209 0.11224007561436673 No Hit GATCTTGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 203 0.10901787248668157 No Hit GATCTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC 192 0.10311050008592541 No Hit GATCTTGGGGAATATTGATGTCAGTATTTCAACTGCTGTAAAGTGATAAACTTTTGTA 191 0.10257346623131122 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGGGG 190 0.10203643237669702 No Hit GATCTTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 189 0.10149939852208284 No Hit GATCTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 189 0.10149939852208284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.009666609383055507 0.0 0.0 0.0 0.0 11 0.01987025262072521 0.0 0.0 0.0 0.0 12 0.02201838803918199 0.0 0.0 0.0 0.0 13 0.02309245574841038 0.0 0.0 0.0 0.0 14 0.05531448702526207 0.0 0.0 0.0 0.0 15 0.05638855473449046 0.0 0.0 0.0 0.0 16 0.06014779171678983 0.0 0.0 0.0 0.0 17 0.06336999484447499 0.0 0.0 0.0 0.0 18 0.06605516411754597 0.0 0.0 0.0 0.0 19 0.07196253651830212 0.0 0.0 0.0 0.0 20 0.10901787248668156 0.0 0.0 0.0 0.0 21 0.11761041416050867 0.0 0.0 0.0 0.0 22 0.1256659219797216 0.0 0.0 0.0 0.0 23 0.14123990376353326 0.0 0.0 0.0 0.0 24 0.15896202096580167 0.0 0.0 0.0 0.0 25 0.16594346107578622 0.0 0.0 0.0 0.0 26 0.17131379962192816 0.0 0.0 0.0 0.0 27 0.1772211720226843 0.0 0.0 0.0 0.0 28 0.24649853926791546 0.0 0.0 0.0 0.0 29 0.2513318439594432 0.0 0.0 0.0 0.0 30 0.29107234920089364 0.0 0.0 0.0 0.0 31 0.29697972160164976 0.0 0.0 0.0 0.0 32 0.3023500601477917 0.0 0.0 0.0 0.0 33 0.31040556796700464 0.0 0.0 0.0 0.0 34 0.316849974222375 0.0 0.0 0.0 0.0 35 0.4554047087128373 0.0 0.0 0.0 0.0 36 0.46614538580512116 0.0 0.0 0.0 0.0 37 0.4747379274789483 0.0 0.0 0.0 0.0 38 0.4784971644612476 0.0 0.0 0.0 0.0 39 0.48708970613507474 0.0 0.0 0.0 0.0 40 0.5005155525004297 0.0 0.0 0.0 0.0 41 0.5123302973019419 0.0 0.0 0.0 0.0 42 0.52360800824884 0.0 0.0 0.0 0.0 43 0.5386449561780374 0.0 0.0 0.0 0.0 44 0.5531448702526207 0.0 0.0 0.0 0.0 45 0.5697929197456608 0.0 0.0 0.0 0.0 46 0.5864409692387008 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGCGT 15 0.0070441356 52.30805 9 GGACTAC 15 0.0070441356 52.30805 8 AATGGTC 15 0.0070441356 52.30805 10 AAGCGAT 20 3.341228E-4 52.30805 10 GATGGAC 15 0.0070441356 52.30805 9 GGGGTAC 55 0.0 52.30805 7 ACTGTGT 15 0.0070441356 52.30805 10 GACTGTG 20 3.341228E-4 52.30805 9 AGCTATA 20 3.341228E-4 52.30805 10 GACCTAC 20 3.341228E-4 52.30805 9 GAGATAA 20 3.341228E-4 52.30805 9 GAGGCAT 15 0.0070441356 52.30805 9 CCAATGA 15 0.0071275453 52.152622 38 ACGGCGT 15 0.0071275453 52.152622 39 CAATGAT 15 0.0071275453 52.152622 39 CGGTCCT 15 0.0071275453 52.152622 38 TCGGTCA 15 0.0071275453 52.152622 39 TGACGGA 20 3.390698E-4 52.15262 38 CTGACGG 25 1.6396378E-5 52.05419 37 GCTGACG 25 1.6396378E-5 52.05419 36 >>END_MODULE