FastQCFastQC Report
Tue 31 May 2016
SRR212649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217310
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT72793.3495927476876353No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGT44162.032120012884819No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC30981.425613179329069No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC16830.774469651649717No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC15400.7086650407252313No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGATCTTGGGAAGCAGT12640.5816575399199301No Hit
GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA11740.540242050526897No Hit
GATCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8430.38792508398140907No Hit
GATCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA7350.33822649670976945No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA7290.33546546408356726No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6840.31475771938705077No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA6440.29635083521236943No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC6230.28668722102066174No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6040.2779439510376881No Hit
GATCTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5830.2682803368459804No Hit
GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5530.2544751737149694No Hit
GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5030.23146656849661776No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4990.22962588007914958No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGG4840.2227232985136441No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.2116791680088353No Hit
GATCTTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC4310.19833417698219133No Hit
GATCTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4250.19557314435598916No Hit
GATCTTGGGAGTGGCACCAGGACAAGTGGCCAGAGGTGCCTCAGCGTGTTGTAGGA4240.1951129722516221No Hit
GATCTTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT3500.16106023652846163No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG3480.16013989231972758Illumina Single End Adapter 2 (95% over 23bp)
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.15875937600662646No Hit
GATCTTGGGAGTATTAAGATACCACGCTGCTCACATCTGCTTCCCTCAAGGGGAAC3410.15691868758915833No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3320.15277713864985504No Hit
GATCTTGGGATTTGACATTATTTTAGTAAACTTACCAAAATGAGTACATTATTTAA3150.1449542128756155No Hit
GATCTTGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3100.14265335235378032No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.14173300814504625No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC3030.13943214762321107No Hit
GATCTTGGGATTTGTTGTTCTGATTATTATGTGTCGGGAGGAATTTCTTTTCTGGT3010.13851180341447702No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2950.1357507707882748No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA2800.1288481892227693No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2720.12516681238783303No Hit
GATCTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA2540.11688371450922644No Hit
GATCTTGGGGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGTGCTTGTCCA2510.11550319819612535No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.11228199346555612No Hit
GATCTTGGGGTGCTGATCTTGGGGTAACACAAATGTATTGGAAAGAAGCATGACAT2400.110441305048088No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGATCTTGGGAAGCAGG2350.10814044452625282No Hit
GATCTTGGGATTTTTAAATTCATTAGGAATATATAGAAGTTGGATTGCAGGTTCAA2340.10768027242188578No Hit
GATCTTGGGGAATATTGATGTCAGTATTTCAACTGCTGTAAAGTGATAAACTTTTG2340.10768027242188578No Hit
GATCTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG2320.10675992821315172Illumina Single End Adapter 2 (96% over 30bp)
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA2260.10399889558694952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCCC252.0081661E-550.3052150
GAAACAC252.0081661E-550.3052150
TGGAGTA150.00822159750.30520650
ATCTAGC150.00822159750.30520648
TTGTCCA301.0009753E-650.30520650
TGATTCG150.00822159750.30520648
TATACAT150.00822159750.30520648
GGTAGGC355.016227E-850.30520648
AGGCCCT204.0527273E-450.30520248
CTAATGC204.0527273E-450.30520249
AAGGGAT150.0082367250.2818347
CCGTAAT150.00824428950.27015346
AGGTAGC150.008251863550.25847643
CAAGGGA301.0074145E-650.25847644
CTAATGA150.008251863550.25847642
TTAGACA204.071362E-450.25847243
TGGCCTC204.071362E-450.25847244
TGAGTAC301.0106487E-650.2351441
TCTAATG150.00826702750.2351441
GGAATTT355.09026E-850.21183440
Produced by FastQC (version 0.11.5)