##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212649_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 217310 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.59815470986149 39.0 38.0 40.0 33.0 40.0 2 36.2852790943813 38.0 35.0 39.0 30.0 40.0 3 37.26678937922783 39.0 37.0 40.0 33.0 40.0 4 37.020657125765034 39.0 36.0 40.0 33.0 40.0 5 37.25608117435921 39.0 37.0 40.0 33.0 40.0 6 37.29334130964981 39.0 37.0 40.0 33.0 40.0 7 37.094091390179926 38.0 36.0 40.0 33.0 40.0 8 36.951571487736416 39.0 36.0 40.0 32.0 40.0 9 36.852040863282866 39.0 36.0 40.0 31.0 40.0 10 35.70435782982835 38.0 35.0 39.0 29.0 40.0 11 35.45456720813584 38.0 35.0 39.0 28.0 40.0 12 35.4873866826193 38.0 35.0 39.0 29.0 40.0 13 35.44480695780222 38.0 35.0 39.0 29.0 40.0 14 35.243016888316234 38.0 35.0 39.0 28.0 40.0 15 35.385780681975056 38.0 35.0 39.0 29.0 40.0 16 34.73957019925452 38.0 35.0 39.0 27.0 40.0 17 34.59971469329529 38.0 34.0 39.0 26.0 40.0 18 34.39173991072661 38.0 34.0 39.0 25.0 40.0 19 33.875017256453916 38.0 33.0 39.0 23.0 40.0 20 34.156992315125855 38.0 33.0 39.0 25.0 40.0 21 33.68623165063734 38.0 33.0 39.0 22.0 40.0 22 33.62064792232295 38.0 33.0 39.0 22.0 40.0 23 33.22957986286871 38.0 33.0 39.0 20.0 40.0 24 33.22386912705352 38.0 33.0 39.0 19.0 40.0 25 33.05745248723022 38.0 33.0 39.0 18.0 40.0 26 32.38952188118356 38.0 33.0 39.0 2.0 40.0 27 32.386392710873864 38.0 33.0 39.0 2.0 40.0 28 31.56104182964429 36.0 31.0 39.0 2.0 40.0 29 31.620795177396346 37.0 31.0 39.0 2.0 40.0 30 30.770466154341722 36.0 30.0 39.0 2.0 40.0 31 29.721798352583868 36.0 29.0 39.0 2.0 40.0 32 29.205793566793982 35.0 26.0 39.0 2.0 40.0 33 29.348014357369657 36.0 27.0 39.0 2.0 40.0 34 29.135810593161843 35.0 27.0 39.0 2.0 40.0 35 28.538562422345958 35.0 25.0 39.0 2.0 40.0 36 28.579177212277393 36.0 24.0 39.0 2.0 40.0 37 28.31513506051263 35.0 23.0 39.0 2.0 40.0 38 28.16490727532097 35.0 23.0 39.0 2.0 40.0 39 28.112231374534076 35.0 22.0 39.0 2.0 40.0 40 27.86309879895081 35.0 21.0 39.0 2.0 40.0 41 27.820808982559477 35.0 19.0 39.0 2.0 40.0 42 27.684653260319358 35.0 18.0 39.0 2.0 40.0 43 27.440808062215268 35.0 17.0 39.0 2.0 40.0 44 27.418770420137133 35.0 15.0 39.0 2.0 40.0 45 27.074253370760665 35.0 8.0 39.0 2.0 40.0 46 26.583406193916524 35.0 2.0 39.0 2.0 40.0 47 26.212797386222448 34.0 2.0 39.0 2.0 40.0 48 26.196424462749068 34.0 2.0 39.0 2.0 40.0 49 25.977161658460265 34.0 2.0 39.0 2.0 40.0 50 25.622672679582163 34.0 2.0 39.0 2.0 40.0 51 25.039896921448623 34.0 2.0 38.0 2.0 40.0 52 24.82120473056923 33.0 2.0 38.0 2.0 40.0 53 24.527384841930882 33.0 2.0 38.0 2.0 39.0 54 24.1710643780774 33.0 2.0 38.0 2.0 39.0 55 23.725898486033778 33.0 2.0 38.0 2.0 39.0 56 13.45741107174083 17.0 2.0 22.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.0828774688417369 1 2 -1.3746408034407835 1 3 -1.9711368135828735 1 4 -1.8952305710147357 1 5 -1.8603513876230764 1 6 -1.705993882465421 1 7 -2.4293988607060513 1 8 -1.8923215788393435 1 9 -2.1998225982567305 1 10 -2.2096074005110964 1 11 -1.7184882898880929 1 12 -2.3586804212239727 1 13 -2.3852260225912687 1 14 -1.3214380568666115 1 15 -1.9467667675477571 1 16 -2.6769612144046704 1 17 -2.8904542587225635 1 18 -2.3281184235539953 1 19 -2.5878084906809775 1 20 -2.591147596496807 1 21 -2.66628446374175 1 22 -2.5057155700125016 1 23 -3.055630867187716 1 24 -2.6436160087984817 1 25 -2.7149540203221996 1 26 -3.37023065853926 1 27 -2.481809065512895 1 28 -2.8970397995989217 1 29 -3.338917999360973 1 30 -3.367925563128235 1 31 -2.633743651442 1 32 -2.5605798634347217 1 33 -2.262536415181252 1 34 -3.3601378012242584 1 35 -3.178311673642469 1 36 -3.491811684999842 1 37 -3.802524353338118 1 38 -3.0671514295327498 1 39 -3.545169493747956 1 40 -3.7922659985548997 1 41 -3.388099970226552 1 42 -3.3310663301972987 1 43 -2.981536253186711 1 44 -3.336255826216995 1 45 -3.63662872627215 1 46 -3.4607938242387775 1 47 -3.347590063313671 1 48 -3.450384220451074 1 49 -3.4291676241427567 1 50 -3.3488053913786615 1 51 -3.6134051617778162 1 52 -3.665399681778883 1 53 -3.7177571229332678 1 54 -2.799817977843336 1 55 -3.0628294745897264 1 56 -1.5768831477250256 2 1 -0.5003528628812433 2 2 -0.3824871796538716 2 3 -0.6598628098661692 2 4 -0.5951410947947622 2 5 -0.8757000007416664 2 6 -0.688614847432035 2 7 -1.0530825278545137 2 8 -0.9231908747303166 2 9 -0.6791054119397089 2 10 -0.4814774535085533 2 11 -2.1942264687028796 2 12 -1.987526177986311 2 13 -1.6646671403557391 2 14 -0.6973070086182744 2 15 -1.3960750475394974 2 16 -1.2243491970600502 2 17 -0.7219980676564219 2 18 -1.0033885729104526 2 19 -0.4202126478838153 2 20 -0.41835525015500963 2 21 -0.842965584259332 2 22 -0.06649057173319761 2 23 -0.6116567464636447 2 24 -0.07258565571154918 2 25 -0.48007894289085584 2 26 -0.03445393612202707 2 27 -0.5564184673987782 2 28 -0.8741545356440703 2 29 -0.6804074342842306 2 30 -1.0183488544765744 2 31 -1.3348586627985988 2 32 -0.6296486224683768 2 33 -1.6882711526029617 2 34 -1.089919616903245 2 35 -0.14912866035869143 2 36 -1.136419022049882 2 37 -1.2063374168662335 2 38 -1.2754589524180133 2 39 -1.3297382871955428 2 40 -1.006079750361632 2 41 -0.8560606006897657 2 42 -1.268123250838773 2 43 -0.720816313755229 2 44 -1.0311419161061828 2 45 -1.236532367266026 2 46 -0.8994681996259359 2 47 -1.1614451118373132 2 48 -1.2021909518159326 2 49 -1.6045410152914918 2 50 -1.8229605293785234 2 51 -1.6371851879324026 2 52 -1.0059255266408833 2 53 -1.078311875497107 2 54 -0.5484242136475928 2 55 -1.0366060593512358 2 56 -1.4803176580147905 3 1 -0.18408131115710802 3 2 -0.03173286930904595 3 3 -0.2263139592914527 3 4 -0.04970287640395554 3 5 -0.0915764375232726 3 6 -0.6623935830642225 3 7 -0.5001012060154366 3 8 -0.5889595528912395 3 9 -0.7994358717098251 3 10 -0.8514487178763659 3 11 -1.0782063537603506 3 12 -1.0227273274115944 3 13 -0.9719588070224034 3 14 -0.9814305718366683 3 15 -1.7060606797234001 3 16 -1.959801783266947 3 17 -1.6257336998403318 3 18 -1.917720469462182 3 19 -1.6711106363895567 3 20 -2.0155176064768447 3 21 -1.856435411845542 3 22 -1.4219144223079105 3 23 -1.7436610568084738 3 24 -1.437528184447185 3 25 -1.5589223336954525 3 26 -1.729137844168001 3 27 -1.6600463409619977 3 28 -1.4375525241498188 3 29 -1.8883815722152626 3 30 -1.3168402337869196 3 31 -1.2921144099250341 3 32 -1.6427592546522867 3 33 -2.3078832215684777 3 34 -1.9290000766733613 3 35 -1.9558815339219073 3 36 -2.314759539291259 3 37 -2.4227526467512916 3 38 -2.5739503317283585 3 39 -2.779450930873704 3 40 -1.8816185434650805 3 41 -2.065651117931143 3 42 -1.9651419289996923 3 43 -2.0744441873184485 3 44 -1.9357755367958376 3 45 -2.167566850024645 3 46 -1.9274854410052455 3 47 -1.876064364710878 3 48 -1.5994610667584617 3 49 -1.6582407279351727 3 50 -1.6498283454704783 3 51 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5 20 -1.5280757706054402 5 21 -0.9891818162448658 5 22 -1.0787725018341376 5 23 -1.507012368145194 5 24 -1.051318370572396 5 25 -1.231960616267365 5 26 -1.1767901284319606 5 27 -1.136965757205651 5 28 -1.546451635824944 5 29 -0.8048896094403055 5 30 -0.398534396223468 5 31 -0.6575437669470325 5 32 -0.4688749483917114 5 33 -0.7500892283366696 5 34 -0.6751929700743773 5 35 -0.7872055609946678 5 36 -0.752761865856403 5 37 -0.7502431289847955 5 38 -0.5735801963645706 5 39 -0.752166666914313 5 40 -0.7952448125006129 5 41 -1.0180887236841052 5 42 -0.5315283503194905 5 43 -0.890196090871747 5 44 -0.6678768481325577 5 45 -0.7479391004476597 5 46 -0.035036202426564245 5 47 -0.011348840176189867 5 48 -0.1881878011070235 5 49 -0.34682469578627106 5 50 -0.34959568895609294 5 51 -0.1624492056697271 5 52 -0.24447503361328415 5 53 -0.07968301733735217 5 54 -0.0911338233097645 5 55 0.18219317047547534 5 56 0.035931831453673624 6 1 -0.34433350413956276 6 2 -0.9305815535195734 6 3 -0.5277393978879488 6 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120 44 -2.3845145718835568 120 45 -2.051338779849207 120 46 -2.3386696515315606 120 47 -1.8154832243600012 120 48 -2.1597680843023213 120 49 -2.018712219163241 120 50 -2.960135363014338 120 51 -3.017478593861018 120 52 -2.688169930754615 120 53 -2.861509092677988 120 54 -2.926827117458842 120 55 -2.731479017608951 120 56 -0.6351875914691156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 627.0 3 63.0 4 312.0 5 227.0 6 748.0 7 3061.0 8 1185.0 9 1661.0 10 3139.0 11 1841.0 12 2170.0 13 2755.0 14 2122.0 15 2326.0 16 2766.0 17 2911.0 18 3096.0 19 3448.0 20 4083.0 21 3640.0 22 2850.0 23 3401.0 24 2972.0 25 2904.0 26 2881.0 27 3091.0 28 3643.0 29 4189.0 30 5123.0 31 6249.0 32 7566.0 33 9499.0 34 11799.0 35 14657.0 36 19651.0 37 27275.0 38 29021.0 39 18358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 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21.400303713588883 34.30583038056233 23 24.644977221480836 39.116929731719665 20.727992269108647 15.510100777690855 24 23.008145046247297 38.63236850582118 23.032994339883118 15.32649210804841 25 20.27703228360593 19.510106752719455 21.89328410770102 38.319576855973594 26 40.793351137305464 16.765816373515058 24.993093286674647 17.447739202504835 27 20.073076781304138 16.14185926499318 23.76377677024586 40.02128718345681 28 23.708656265263432 40.341992977670465 20.70481333689672 15.244537420169385 29 44.42723744734198 19.84227943548759 21.540241328134364 14.19024178903607 30 21.333376374234486 37.97729285029145 24.925754445510222 15.763576329963847 31 39.80648791241226 21.227759484213728 24.76594998939281 14.199802613981202 32 26.25968500376094 21.44890886521059 40.50327409656624 11.788132034462231 33 37.44806190098059 20.529630108400585 25.150505069158463 16.87180292146036 34 23.300840863859477 23.089355060652096 30.573089585940348 23.03671448954808 35 17.025708769434 42.00838791327563 30.65745351082207 10.3084498064683 36 28.33766185753764 31.125169829108017 25.71466861373236 14.822499699621986 37 20.54902794597543 29.27625192977915 38.014569254804805 12.160150869440619 38 28.275262612812547 28.662708980618433 31.0183644030182 12.04366400355082 39 21.254716283198935 25.49151069024192 30.5948065399127 22.658966486646445 40 18.64278179765716 26.936173362695932 39.47480430075689 14.94624053889002 41 26.98992833713469 25.98923900421465 33.645923451661105 13.374909206989557 42 18.340811535416115 32.51513004662879 32.92677844610832 16.217279971846768 43 15.604482103995926 25.936472658239566 40.438023799601794 18.021021438162705 44 17.198286838754488 23.803680981595093 33.76640814909133 25.23162403055909 45 17.51371335384159 24.317575330788333 42.53409806901153 15.634613246358548 46 17.89685849318877 26.450745992030395 41.74636270966546 13.906032805115373 47 24.78061534325065 24.934984261787434 35.11823361163005 15.166166783331866 48 28.889280562525798 24.125807633616112 33.27056247941781 13.714349324440281 49 29.332208606017907 24.511485196240198 32.17060012236044 13.985706075381449 50 15.577784579426249 31.22188523145701 35.898327721973345 17.302002467143403 51 15.44233587474725 32.25462370378615 36.71925723931957 15.583783182147032 52 22.329966454941516 25.51048341073906 37.5932000501088 14.566350084210624 53 12.931606520558878 26.71378124666478 39.44585458207078 20.90875765070556 54 13.65434253774303 32.26541754099274 36.81222261425043 17.268017307013796 55 21.263621006515805 26.610852252687074 34.72915777163118 17.39636896916594 56 15.886275984016262 28.282490752730087 36.696694188027045 19.1345390752266 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 361.5 9 723.0 10 711.0 11 699.0 12 707.0 13 665.5 14 616.0 15 898.5 16 1181.0 17 1463.0 18 1745.0 19 1793.0 20 1841.0 21 2144.0 22 3210.0 23 3973.0 24 4940.0 25 5907.0 26 6432.0 27 6957.0 28 8069.0 29 9487.5 30 9794.0 31 8495.5 32 7197.0 33 10518.0 34 13839.0 35 9809.0 36 5779.0 37 5334.5 38 4622.5 39 4355.0 40 4617.0 41 4879.0 42 5108.5 43 5338.0 44 5911.5 45 6485.0 46 6240.5 47 6164.0 48 6332.0 49 7380.0 50 8428.0 51 12370.5 52 16313.0 53 14505.5 54 12250.0 55 11802.0 56 11674.5 57 11547.0 58 11687.5 59 11828.0 60 12509.0 61 13190.0 62 11817.0 63 7407.5 64 4371.0 65 3378.0 66 2385.0 67 1866.5 68 1348.0 69 1063.5 70 779.0 71 680.0 72 438.0 73 295.0 74 247.0 75 199.0 76 158.5 77 118.0 78 87.5 79 41.5 80 26.0 81 20.0 82 14.0 83 13.0 84 12.0 85 7.5 86 3.0 87 1.5 88 1.0 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.03635359624499563 26 0.059822373567714325 27 0.12792784501403526 28 0.13252956605770558 29 0.15829920390225946 30 0.2125995122175694 31 0.2190419216787078 32 0.28024481155952324 33 0.3248815056831255 34 0.34374856196217385 35 0.3718190603285629 36 0.42151764760020244 37 0.4436059086098201 38 0.470295890663108 39 0.47765864433298055 40 0.5347199852744926 41 0.5333394689613915 42 0.6207721687911278 43 0.6166306198518245 44 0.6143297593299895 45 0.6727716165846026 46 0.6856564355068795 47 0.7330541622566841 48 0.7873544705719939 49 0.7151074501863697 50 0.7712484469191477 51 0.7740094795453499 52 0.8186461736689523 53 0.8315309925912291 54 0.8770880309235655 55 0.8439556394091391 56 0.8471768441397083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 217310.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.940131609221844 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83148807984983 31.045511021121904 2 11.014196275023323 9.018452901385118 3 4.394887992176875 5.3978187842253 4 2.414378365011746 3.95379872072155 5 1.3993952813964727 2.8645713496847818 6 0.9318061753234346 2.2888960471216238 7 0.655299155866782 1.8779623579218627 8 0.5170456461384558 1.6934333440706824 9 0.393404295974912 1.449542128756155 >10 2.186203873346297 15.517463531360729 >50 0.1337574606314701 3.9027196171368095 >100 0.10902919059876134 8.585891123280106 >500 0.011240122742140344 2.9915788504900833 >1k 0.006744073645284206 6.062767475035663 >5k 0.0011240122742140345 3.3495927476876353 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 7279 3.3495927476876353 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGT 4416 2.032120012884819 No Hit GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 3098 1.425613179329069 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1683 0.774469651649717 No Hit GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1540 0.7086650407252313 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGATCTTGGGAAGCAGT 1264 0.5816575399199301 No Hit GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1174 0.540242050526897 No Hit GATCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 843 0.38792508398140907 No Hit GATCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 735 0.33822649670976945 No Hit GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 729 0.33546546408356726 No Hit GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 684 0.31475771938705077 No Hit GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 644 0.29635083521236943 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 623 0.28668722102066174 No Hit GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 604 0.2779439510376881 No Hit GATCTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 583 0.2682803368459804 No Hit GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 553 0.2544751737149694 No Hit GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 503 0.23146656849661776 No Hit GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 499 0.22962588007914958 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGG 484 0.2227232985136441 No Hit GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 0.2116791680088353 No Hit GATCTTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 431 0.19833417698219133 No Hit GATCTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 425 0.19557314435598916 No Hit GATCTTGGGAGTGGCACCAGGACAAGTGGCCAGAGGTGCCTCAGCGTGTTGTAGGA 424 0.1951129722516221 No Hit GATCTTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT 350 0.16106023652846163 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 348 0.16013989231972758 Illumina Single End Adapter 2 (95% over 23bp) GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 345 0.15875937600662646 No Hit GATCTTGGGAGTATTAAGATACCACGCTGCTCACATCTGCTTCCCTCAAGGGGAAC 341 0.15691868758915833 No Hit GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 332 0.15277713864985504 No Hit GATCTTGGGATTTGACATTATTTTAGTAAACTTACCAAAATGAGTACATTATTTAA 315 0.1449542128756155 No Hit GATCTTGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 310 0.14265335235378032 No Hit GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 308 0.14173300814504625 No Hit GATCTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC 303 0.13943214762321107 No Hit GATCTTGGGATTTGTTGTTCTGATTATTATGTGTCGGGAGGAATTTCTTTTCTGGT 301 0.13851180341447702 No Hit GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 295 0.1357507707882748 No Hit GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 280 0.1288481892227693 No Hit GATCTTGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 272 0.12516681238783303 No Hit GATCTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 254 0.11688371450922644 No Hit GATCTTGGGGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGTGCTTGTCCA 251 0.11550319819612535 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 244 0.11228199346555612 No Hit GATCTTGGGGTGCTGATCTTGGGGTAACACAAATGTATTGGAAAGAAGCATGACAT 240 0.110441305048088 No Hit GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGATCTTGGGAAGCAGG 235 0.10814044452625282 No Hit GATCTTGGGATTTTTAAATTCATTAGGAATATATAGAAGTTGGATTGCAGGTTCAA 234 0.10768027242188578 No Hit GATCTTGGGGAATATTGATGTCAGTATTTCAACTGCTGTAAAGTGATAAACTTTTG 234 0.10768027242188578 No Hit GATCTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 232 0.10675992821315172 Illumina Single End Adapter 2 (96% over 30bp) GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA 226 0.10399889558694952 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.03359256361879343 0.0 0.0 0.0 0.0 11 0.05752151304587916 0.0 0.0 0.0 0.0 12 0.060282545672081356 0.0 0.0 0.0 0.0 13 0.06166306198518246 0.0 0.0 0.0 0.0 14 0.08375132299480005 0.0 0.0 0.0 0.0 15 0.08743269982973632 0.0 0.0 0.0 0.0 16 0.08973356035157148 0.0 0.0 0.0 0.0 17 0.09341493718650776 0.0 0.0 0.0 0.0 18 0.09709631402144402 0.0 0.0 0.0 0.0 19 0.10307855137821545 0.0 0.0 0.0 0.0 20 0.13206939395333855 0.0 0.0 0.0 0.0 21 0.13805163131010997 0.0 0.0 0.0 0.0 22 0.14863558971055174 0.0 0.0 0.0 0.0 23 0.16704247388523308 0.0 0.0 0.0 0.0 24 0.1909714233123188 0.0 0.0 0.0 0.0 25 0.19603331646035618 0.0 0.0 0.0 0.0 26 0.20247572592149463 0.0 0.0 0.0 0.0 27 0.21259951221756937 0.0 0.0 0.0 0.0 28 0.3023330725691409 0.0 0.0 0.0 0.0 29 0.3106161704477475 0.0 0.0 0.0 0.0 30 0.3483502830058442 0.0 0.0 0.0 0.0 31 0.35157148773641345 0.0 0.0 0.0 0.0 32 0.359394413510653 0.0 0.0 0.0 0.0 33 0.36537665086742444 0.0 0.0 0.0 0.0 34 0.37227923243292993 0.0 0.0 0.0 0.0 35 0.5098706916386728 0.0 0.0 0.0 0.0 36 0.5222953384565827 0.0 0.0 0.0 0.0 37 0.5291979200220882 0.0 0.0 0.0 0.0 38 0.53978187842253 0.0 0.0 0.0 0.0 39 0.5512861810317059 0.0 0.0 0.0 0.0 40 0.5604896231190465 0.0 0.0 0.0 0.0 41 0.5715337536238553 0.0 0.0 0.0 0.0 42 0.5890202935898026 0.0 0.0 0.0 0.0 43 0.6101882103906862 0.0 0.0 0.0 0.0 44 0.6244535456260641 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATCCC 25 2.0081661E-5 50.30521 50 GAAACAC 25 2.0081661E-5 50.30521 50 TGGAGTA 15 0.008221597 50.305206 50 ATCTAGC 15 0.008221597 50.305206 48 TTGTCCA 30 1.0009753E-6 50.305206 50 TGATTCG 15 0.008221597 50.305206 48 TATACAT 15 0.008221597 50.305206 48 GGTAGGC 35 5.016227E-8 50.305206 48 AGGCCCT 20 4.0527273E-4 50.305202 48 CTAATGC 20 4.0527273E-4 50.305202 49 AAGGGAT 15 0.00823672 50.28183 47 CCGTAAT 15 0.008244289 50.270153 46 AGGTAGC 15 0.0082518635 50.258476 43 CAAGGGA 30 1.0074145E-6 50.258476 44 CTAATGA 15 0.0082518635 50.258476 42 TTAGACA 20 4.071362E-4 50.258472 43 TGGCCTC 20 4.071362E-4 50.258472 44 TGAGTAC 30 1.0106487E-6 50.23514 41 TCTAATG 15 0.008267027 50.23514 41 GGAATTT 35 5.09026E-8 50.211834 40 >>END_MODULE