FastQCFastQC Report
Tue 31 May 2016
SRR212648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197908
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT64293.248479091294945No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC30621.547183539826586No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGT30501.5411201164177295No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC15260.7710653434929361No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC14600.7377165147442246No Hit
GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA12410.6270590375325908No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGATCTTGGGAAGCAGT10640.5376235422519554No Hit
GATCTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8990.4542514703801766No Hit
GATCTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA7270.3673424015198981No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA6780.3425834226004002No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6050.3056975968631889No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC5890.29761303231804676No Hit
GATCTTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC5700.2880126119206904No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5580.2819491885118338No Hit
GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5550.2804333326596196No Hit
GATCTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5480.2768963356711199No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5280.26679062998969216No Hit
GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5010.2531479273197647No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.24354750692240837No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4690.23697879822948037No Hit
GATCTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4450.2248519514117671No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGG3870.19554540493562667No Hit
GATCTTGGGAGTGGCACCAGGACAAGTGGCCAGAGGTGCCTCAGCGTGTTGTAGGA3610.18240798754977058No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG3400.17179699658427147Illumina Single End Adapter 2 (95% over 23bp)
GATCTTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT3210.16219657618691513No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190.16118600561877236No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3030.1531014410736302No Hit
GATCTTGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2890.14602744709663076No Hit
GATCTTGGGAGTATTAAGATACCACGCTGCTCACATCTGCTTCCCTCAAGGGGAAC2770.1399640236877741No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA2760.13945873840370274No Hit
GATCTTGGGGTGCTGATCTTGGGGTAACACAAATGTATTGGAAAGAAGCATGACAT2740.13844816783555994No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC2710.1369323119833458No Hit
GATCTTGGGATTTGTTGTTCTGATTATTATGTGTCGGGAGGAATTTCTTTTCTGGT2710.1369323119833458No Hit
GATCTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA2610.13187945914263194No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.13137417385856054No Hit
GATCTTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2590.13086888857448917No Hit
GATCTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG2490.12581603573377528Illumina Single End Adapter 2 (96% over 30bp)
GATCTTGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2410.12177375346120418No Hit
GATCTTGGGATTTGACATTATTTTAGTAAACTTACCAAAATGAGTACATTATTTAA2200.11116276249570507No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2180.11015219192756229No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2170.10964690664349092No Hit
GATCTTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2130.10762576550720536No Hit
GATCTTGGGATTTTTAAATTCATTAGGAATATATAGAAGTTGGATTGCAGGTTCAA2100.1061099096549912No Hit
GATCTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2040.10307819795056289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATAA252.0334817E-550.19608749
TTGATTC150.00829141150.19608347
TTATCTC150.00829141150.19608347
AGCATGA301.0156255E-650.19608347
ATGAATA204.0955146E-450.19608348
CAGCTCG150.00829141150.19608349
CTAATGC204.0955146E-450.19608349
TACAACT204.0955146E-450.19608347
CGGGTAT150.00829975150.18332346
GCAAGAA150.00829975150.18332345
CAATTAT150.00829975150.18332344
CGCGAGA150.00829975150.18332346
CCTCCGT204.1006587E-450.18332344
ACTACTG150.00829975150.18332345
TAGGGCT204.1006587E-450.18332344
AATTATC150.00829975150.18332345
CTACAAC204.1006587E-450.18332346
GTCGCGT355.109905E-850.1833244
CGTGTTG451.3096724E-1050.1833245
GCGTGTT451.3096724E-1050.1833244
Produced by FastQC (version 0.11.5)