FastQCFastQC Report
Tue 31 May 2016
SRR212647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303990
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT87552.8800289483206685No Hit
AAGTACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC64092.10829303595513No Hit
AAGTACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC43131.418796670943123No Hit
AAGTACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT37511.2339221684923847No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA30931.01746767985789No Hit
AAGTACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC28050.922727721306622No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT27200.8947662752064214No Hit
AAGTACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG21680.713181354649824No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG20770.6832461594131386No Hit
AAGTACGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT16840.5539655909733873No Hit
AAGTACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG12640.41580315141945456No Hit
AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12010.39507878548636466No Hit
AAGTACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG9690.3187604855422876No Hit
AAGTACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT9250.30428632520806603No Hit
AAGTACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA8770.288496332116188No Hit
AAGTACGGGGATTAAACCCCACCCTATCCCCACCTCCAGAGACAGGGTTTCTTTGTGT8190.26941675713016877No Hit
AAGTACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7950.2615217605842297No Hit
AAGTACGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7910.26020592782657326No Hit
AAGTACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC7710.2536267640382907No Hit
AAGTACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC7550.24836343300766472No Hit
AAGTACGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC7120.2342182308628573No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA6500.21382282311918155No Hit
AAGTACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC6490.2134938649297674No Hit
AAGTACGGGAACGCAGAGTGCATGCTAAGTACGGGGCCTTTTCCTGTGGGAGCAGCCG5740.18882200072370803No Hit
AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC5710.18783512615546563No Hit
AAGTACGGGATCTATGGGCGGCCCCCACTTACTCGTCTGAAGAGCAAATCACTATATG5690.18717720977663738No Hit
AAGTACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5640.18553241882956678No Hit
AAGTACGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC5490.18059804598835488No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.175663673147143No Hit
AAGTACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5340.175663673147143No Hit
AAGTACGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5340.175663673147143No Hit
AAGTACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTT5250.17270304944241588No Hit
AAGTACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG5020.165137011085891No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA4980.16382117832823448No Hit
AAGTACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC4970.16349222013882037No Hit
AAGTACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4930.16217638738116386No Hit
AAGTACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4840.15921576367643672No Hit
AAGTACGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC4820.15855784729760847No Hit
AAGTACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4770.15691305635053784No Hit
AAGTACGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4740.15592618178229548No Hit
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT4730.15559722359288133No Hit
AAGTACGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA4710.1549393072140531No Hit
AAGTACGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA4680.1539524326458107No Hit
AAGTACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA4630.1523076416987401No Hit
AAGTACGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4570.15033389256225532No Hit
AAGTACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG4480.14737326885752822No Hit
AAGTACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4320.1421099378269022No Hit
AAGTACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG4300.14145202144807395No Hit
AAGTACGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG4260.14013618869041744No Hit
AAGTACGGGCTTCTTTTTACCTCGATCTTGCTCCCTTGCTTCCCAAACAGACGTTGTT4200.1381624395539327No Hit
AAGTACGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3930.1292805684397513No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3830.12599098654561006No Hit
AAGTACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3810.1253330701667818No Hit
AAGTACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA3730.1227014046514688No Hit
AAGTACGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG3710.12204348827264055No Hit
AAGTACGGGAACCACCACAGCACTGCTTTCCCATGAGTCACAGGGTGTAGCTTTTACT3660.12039869732556992No Hit
AAGTACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA3640.11974078094674166No Hit
AAGTACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3640.11974078094674166No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.11974078094674166No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.1187539063784993No Hit
AAGTACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT3590.11809598999967105No Hit
AAGTACGGGATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTG3560.11710911543142866No Hit
AAGTACGGGGGGAGAAGCGGGGGCCTGTGCTGGCTTTGTTGCTAAGCTGTGACATCCT3460.11381953353728741No Hit
AAGTACGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA3430.11283265896904504No Hit
AAGTACGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT3420.11250370077963091No Hit
AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.11151682621138855No Hit
AAGTACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA3360.11052995164314615No Hit
AAGTACGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA3290.10822724431724727No Hit
AAGTACGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3260.1072403697490049No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG3260.1072403697490049No Hit
AAGTACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT3260.1072403697490049No Hit
AAGTACGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC3190.10493766242310601No Hit
AAGTACGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAG3180.1046087042336919No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG3160.10395078785486364No Hit
AAGTACGGGGTCAAGGTGATGAGAGACTCCAGTGGGAAGTCCAAAGGCTTTGGCTTTG3130.10296391328662127No Hit
AAGTACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3130.10296391328662127No Hit
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT3100.10197703871837889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAAC150.007181230552.06181349
ACGTTGT550.052.06181351
TCAATCC150.007181230552.06181352
CACTATA600.052.06181350
AAACCAC150.007181230552.06181352
TCCCGGT150.007181230552.06181350
CACATGT251.6407132E-552.0618151
CTAAAAC203.4234842E-452.0618152
CATACCC203.4234842E-452.0618152
GTATCCT353.8346116E-852.0618148
ACATGTT251.6407132E-552.0618152
TCATCAA203.4234842E-452.0618152
TAAGGTC251.6407132E-552.0618148
GTAAACT401.8681021E-952.0618149
ACTATTA150.00718591352.0532347
AAATCAC600.052.0532346
TAACCTT203.426271E-452.0532346
TGTTAGT353.844616E-852.0446644
CCCCTTG150.007190597752.04465542
CAAACAG503.6379788E-1252.04465544
Produced by FastQC (version 0.11.5)