FastQCFastQC Report
Tue 31 May 2016
SRR212646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303781
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT85272.806956327090898No Hit
AAGTACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC63172.079458557315961No Hit
AAGTACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC41381.3621655073885464No Hit
AAGTACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT36861.2133741083214553No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA29390.9674732784473025No Hit
AAGTACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC26300.8657552644832955No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT25730.846991747344304No Hit
AAGTACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG20770.6837162297839562No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG20050.6600149449768089No Hit
AAGTACGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT16590.5461171040980181No Hit
AAGTACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG12000.39502141345245423No Hit
AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10880.35815274819689186No Hit
AAGTACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT9660.31799223782922564No Hit
AAGTACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA9150.3012038277574964No Hit
AAGTACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG8950.2946201375332888No Hit
AAGTACGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8170.26894374565887924No Hit
AAGTACGGGGATTAAACCCCACCCTATCCCCACCTCCAGAGACAGGGTTTCTTTGTGT7850.25840984130014716No Hit
AAGTACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC7240.23832958611631405No Hit
AAGTACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7180.23635447904905177No Hit
AAGTACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC7150.23536692551542066No Hit
AAGTACGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC6970.22944160431363383No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA6890.22680812822395083No Hit
AAGTACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC6190.2037652124392243No Hit
AAGTACGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC5880.19356049259170258No Hit
AAGTACGGGAACGCAGAGTGCATGCTAAGTACGGGGCCTTTTCCTGTGGGAGCAGCCG5790.19059783199080918No Hit
AAGTACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5740.1889519094347573No Hit
AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC5540.1823682192105497No Hit
AAGTACGGGATCTATGGGCGGCCCCCACTTACTCGTCTGAAGAGCAAATCACTATATG5480.18039311214328743No Hit
AAGTACGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5330.17545534447513175No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5320.1751261599639214No Hit
AAGTACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5210.17150513034060721No Hit
AAGTACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG5180.17051757680697607No Hit
AAGTACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTT5070.16689654718366193No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.1655798091388204No Hit
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT5020.16525062462761003No Hit
AAGTACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC4950.16294633304913736No Hit
AAGTACGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4660.1533999822240364No Hit
AAGTACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4610.1517540596679845No Hit
AAGTACGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC4490.14780384553345996No Hit
AAGTACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG4480.1474746610222496No Hit
AAGTACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4460.14681629199982885No Hit
AAGTACGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA4410.14517036944377693No Hit
AAGTACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4330.1425368933540939No Hit
AAGTACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA4300.14154933982046278No Hit
AAGTACGGGATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTG4240.1395742327532005No Hit
AAGTACGGGCTTCTTTTTACCTCGATCTTGCTCCCTTGCTTCCCAAACAGACGTTGTT4180.13759912568593824No Hit
AAGTACGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG4160.1369407566635175No Hit
AAGTACGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4130.13595320312988635No Hit
AAGTACGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA4040.13299054252899292No Hit
AAGTACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3980.13101543546173064No Hit
AAGTACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3890.12805277486083724No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3800.12509011425994385No Hit
AAGTACGGGAACCACCACAGCACTGCTTTCCCATGAGTCACAGGGTGTAGCTTTTACT3750.12344419170389194No Hit
AAGTACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG3730.12278582268147119No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.12113990012541931No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG3680.12113990012541931No Hit
AAGTACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT3550.11686050147968437No Hit
AAGTACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA3470.11422702539000136No Hit
AAGTACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3390.11159354930031831No Hit
AAGTACGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3340.10994762674426642No Hit
AAGTACGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT3320.10928925772184567No Hit
AAGTACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3320.10928925772184567No Hit
AAGTACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA3300.10863088869942492No Hit
AAGTACGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3260.1073141506545834No Hit
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT3260.1073141506545834No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG3230.10632659712095226No Hit
AAGTACGGGGGGAGAAGCGGGGGCCTGTGCTGGCTTTGTTGCTAAGCTGTGACATCCT3190.10500985907611075No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.10435149005369No Hit
AAGTACGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA3150.10369312103126925No Hit
AAGTACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT3110.10237638298642772No Hit
AAGTACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA3100.10204719847521734No Hit
AAGTACGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC3080.10138882945279659No Hit
AAGTACGGGGTCAAGGTGATGAGAGACTCCAGTGGGAAGTCCAAAGGCTTTGGCTTTG3080.10138882945279659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATGC458.913048E-1152.1472527
ACCAGTC150.007134801752.14724726
GCCCTAC307.8213634E-752.14724726
TAATATA150.007139466252.13863425
CATATGT150.007139466252.13863422
GCAACGA150.007139466252.13863423
TTCACCG150.007139466252.13863424
ATAGATG150.007139466252.13863422
GCACATA150.007139466252.13863425
AACATTG150.007139466252.13863425
GTCAATC150.007139466252.13863421
TCCCGTT307.8302946E-752.13863422
TAGATGA150.007139466252.13863423
ACATATG150.007139466252.13863421
TACAGGT203.3986432E-452.1386322
TACATCA353.7902282E-852.13862624
ATACATC353.7902282E-852.13862623
CTATCGG307.929066E-752.0440451
GTTAGCT150.00719092752.0440450
TCGTCGT150.00719092752.0440451
Produced by FastQC (version 0.11.5)