##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212645_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 301568 Sequences flagged as poor quality 0 Sequence length 58 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.60941147601868 39.0 38.0 40.0 33.0 40.0 2 37.32691465938031 39.0 37.0 40.0 33.0 40.0 3 36.99209465195246 38.0 36.0 40.0 33.0 40.0 4 36.81155493951613 38.0 36.0 40.0 31.0 40.0 5 37.29931557724957 39.0 37.0 40.0 33.0 40.0 6 37.59002613009338 39.0 38.0 40.0 33.0 40.0 7 37.75963961693548 39.0 38.0 40.0 34.0 40.0 8 37.62189622241087 39.0 38.0 40.0 34.0 40.0 9 37.55061213391341 39.0 38.0 40.0 34.0 40.0 10 36.516566744482176 39.0 36.0 40.0 31.0 40.0 11 37.21797405560272 39.0 37.0 40.0 33.0 40.0 12 37.2563037192275 39.0 36.0 40.0 33.0 40.0 13 37.23575445670628 39.0 36.0 40.0 32.0 40.0 14 36.998514431239386 39.0 36.0 40.0 32.0 40.0 15 37.26676902058574 39.0 37.0 40.0 33.0 40.0 16 37.16009656196944 39.0 36.0 40.0 33.0 40.0 17 36.687460207979626 38.0 36.0 40.0 31.0 40.0 18 36.75659552737691 38.0 36.0 40.0 31.0 40.0 19 36.70167259125637 38.0 36.0 40.0 31.0 40.0 20 36.591534910865875 38.0 36.0 40.0 31.0 40.0 21 36.57650347516978 38.0 36.0 40.0 31.0 40.0 22 36.57082316426146 38.0 36.0 40.0 31.0 40.0 23 36.307884125636676 38.0 35.0 40.0 30.0 40.0 24 36.12009231748727 38.0 35.0 40.0 30.0 40.0 25 36.15927750955009 38.0 35.0 40.0 30.0 40.0 26 35.92192142402377 38.0 35.0 40.0 30.0 40.0 27 35.75696691956706 38.0 35.0 39.0 29.0 40.0 28 35.506151183149406 38.0 35.0 39.0 29.0 40.0 29 35.291881101443124 38.0 35.0 39.0 28.0 40.0 30 35.164523424235995 38.0 35.0 39.0 28.0 40.0 31 34.70760160229202 38.0 34.0 39.0 27.0 40.0 32 34.55484335207979 38.0 33.0 39.0 26.0 40.0 33 34.241859215831916 38.0 33.0 39.0 25.0 40.0 34 33.7445916012309 37.0 33.0 39.0 23.0 40.0 35 33.23686531727504 37.0 32.0 39.0 22.0 40.0 36 34.17147707979626 38.0 33.0 39.0 25.0 40.0 37 33.930480024405774 38.0 33.0 39.0 24.0 40.0 38 33.7749363327674 38.0 33.0 39.0 23.0 40.0 39 33.376565152801355 38.0 33.0 39.0 20.0 40.0 40 33.65136221349746 38.0 33.0 39.0 23.0 40.0 41 33.64258807300509 38.0 33.0 39.0 23.0 40.0 42 33.39544978247029 38.0 33.0 39.0 22.0 40.0 43 33.088278597198645 38.0 33.0 39.0 19.0 40.0 44 32.89520108234296 38.0 33.0 39.0 17.0 40.0 45 32.666446705220714 37.0 32.0 39.0 16.0 40.0 46 32.19004005730051 37.0 32.0 39.0 9.0 40.0 47 32.17141739176571 37.0 31.0 39.0 10.0 40.0 48 32.380501246816635 37.0 32.0 39.0 14.0 40.0 49 32.281422432088284 37.0 32.0 39.0 12.0 40.0 50 31.80541038837012 36.0 31.0 39.0 6.0 40.0 51 32.019209597835314 37.0 32.0 39.0 2.0 40.0 52 31.85148623196095 37.0 31.0 39.0 2.0 40.0 53 31.674007189091682 36.0 31.0 39.0 2.0 40.0 54 31.420014723047537 36.0 31.0 39.0 2.0 40.0 55 31.263240794779286 36.0 31.0 39.0 2.0 40.0 56 31.02563269312394 37.0 31.0 39.0 2.0 40.0 57 30.716259682724957 36.0 30.0 39.0 2.0 40.0 58 30.326685855263158 36.0 30.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.530840529372803 1 2 -3.074723582675908 1 3 -3.586766532543699 1 4 -5.592993913336716 1 5 -2.2841266057305916 1 6 -2.760991326262797 1 7 -4.085585728164887 1 8 -3.6280972384094383 1 9 -3.8735969733350615 1 10 -4.182215303250736 1 11 -3.3227560760411023 1 12 -3.1601053871823055 1 13 -2.8737338333776705 1 14 -3.2890911705549826 1 15 -2.5676642784833135 1 16 -2.3315503390134182 1 17 -2.1390757395704583 1 18 -1.607321271665839 1 19 -2.8191492579481974 1 20 -3.2360916888503155 1 21 -2.377342264992876 1 22 -2.1701459933778935 1 23 -2.359359235413997 1 24 -3.08830914338418 1 25 -2.231216987133827 1 26 -3.681908563550529 1 27 -3.2185223402748946 1 28 -3.307689906879915 1 29 -2.583721726604338 1 30 -2.666266856113083 1 31 -2.926705948187827 1 32 -2.390136209406215 1 33 -2.6193575364748725 1 34 -2.7238489922523534 1 35 -3.1895979239690675 1 36 -2.293970004948296 1 37 -2.5037299553700016 1 38 -2.024942058259061 1 39 -2.0159516287564543 1 40 -2.4011179292771025 1 41 -1.930158296670026 1 42 -2.337300337292376 1 43 -2.0137865755916664 1 44 -1.4741515791882698 1 45 -2.595127252327547 1 46 -2.4290150203763403 1 47 -2.9428730418263136 1 48 -2.8781163149180564 1 49 -3.1065297446901674 1 50 -2.7629290819046872 1 51 -2.584279773968696 1 52 -2.087297109130926 1 53 -2.490180014347615 1 54 -3.304935250381348 1 55 -2.659452962137337 1 56 -2.5494112555559525 1 57 -3.052067869809644 1 58 -2.8438843632551922 2 1 -0.42524014968604007 2 2 -1.1236873542310448 2 3 -0.41717311378084077 2 4 0.1266706966933384 2 5 -1.1521063557701368 2 6 -0.453682356163128 2 7 -0.39352750952859594 2 8 -1.0455153684521576 2 9 -0.865686832534557 2 10 -0.2613167112558017 2 11 -0.5567380409200737 2 12 -0.34235503122597066 2 13 -0.3378217941433732 2 14 -0.5555047127160364 2 15 -0.5268479519527034 2 16 -0.33669193053375324 2 17 -0.5068181853859954 2 18 -0.5523457931023188 2 19 -0.6141975098070844 2 20 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0.15003792524503723 3 42 0.02378592988381456 3 43 -0.03002095779076086 3 44 0.25663954204109984 3 45 0.3858197081299437 3 46 0.5336919747723172 3 47 -0.17747206071673816 3 48 -0.2165178109746435 3 49 -0.19108946499262913 3 50 0.06545804984044068 3 51 -0.054345090932134354 3 52 -0.052389122031705426 3 53 -0.1770109220094831 3 54 0.2183322239390577 3 55 -0.5482515094447074 3 56 -0.20127997116968643 3 57 -0.2841734610501234 3 58 -0.21716674393654856 4 1 -0.6674142428276468 4 2 -0.8737317355553813 4 3 -0.6842895112008094 4 4 -0.7866377830983637 4 5 -0.450585555722796 4 6 -0.2731687163027843 4 7 -0.5697213671533063 4 8 -0.6181528026198606 4 9 -0.6423304209125718 4 10 -0.030504237144903357 4 11 -0.1077173888104852 4 12 -0.5422781035971198 4 13 -0.45001778207165444 4 14 -0.4238992922769569 4 15 -0.2240489697644037 4 16 -0.05827308918219387 4 17 -0.10430916118338018 4 18 -0.4896497755864928 4 19 -0.14144764295105716 4 20 -0.29960106206524983 4 21 -0.7115324296083401 4 22 -0.04811244713496876 4 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17 -0.5217223831335431 118 18 -0.71839815524946 118 19 -0.48681707979392286 118 20 -0.5927596390492624 118 21 -0.2455234997509237 118 22 -0.4386352758833496 118 23 -0.6521072780272661 118 24 -0.47629528123554365 118 25 -0.1880646318520931 118 26 -0.82486838899014 118 27 -0.21551887473773235 118 28 -0.5646017282122244 118 29 -0.04289256475091463 118 30 -0.26996342908189064 118 31 -0.5671115443885739 118 32 -0.5088116040923936 118 33 -0.6195372309941902 118 34 -0.4905798355330617 118 35 0.025496415653570637 118 36 -0.20389170947916568 118 37 -0.03865486873157664 118 38 -0.19359819996102345 118 39 0.2257888410163602 118 40 -0.3184584503912049 118 41 -0.5402725310430228 118 42 -0.4589021855788644 118 43 -0.5799269940231895 118 44 -0.15932678134460332 118 45 0.12249564588834261 118 46 0.4301814884158546 118 47 -0.17118776394157464 118 48 -0.22451600687031004 118 49 0.3497659811473355 118 50 0.41810031098454203 118 51 0.34335467603771974 118 52 0.645137751605823 118 53 0.5971001807492904 118 54 0.4557373205338138 118 55 0.4467721694247224 118 56 0.6356740993407257 118 57 0.5983492781816295 118 58 0.5675134033986389 119 1 -0.15242924267913338 119 2 -0.5473141753332058 119 3 -0.2596006467341212 119 4 0.0831753870682661 119 5 -0.3308986219300678 119 6 0.047976618891915734 119 7 -0.2639917209121805 119 8 -0.13303384340163404 119 9 -0.15899362226701186 119 10 0.36176415623733504 119 11 0.30547653996249835 119 12 -0.16396996803410246 119 13 -0.011561727181103265 119 14 0.03741297195804805 119 15 0.07496787165300844 119 16 0.08262155542529115 119 17 0.03845675202540377 119 18 -0.3377799921190032 119 19 -0.4389644992198072 119 20 -0.24067095246237358 119 21 -0.45013402761231447 119 22 -0.36530732639359087 119 23 -0.5422915523096279 119 24 -0.47932002244397154 119 25 -0.7925870604549843 119 26 -0.3947384425838578 119 27 -0.33573031295812683 119 28 -0.480643134863719 119 29 -0.02759266019177886 119 30 -0.04909858802763267 119 31 -0.18179475413822388 119 32 -0.041768068375482414 119 33 0.13513570050863422 119 34 0.04459379342223002 119 35 -0.31022049206245583 119 36 0.23698634646386552 119 37 -0.08603758659369731 119 38 0.06915783850898549 119 39 0.04177885643373003 119 40 -0.008862972819812853 119 41 0.2757762172133482 119 42 0.13844015345645033 119 43 -0.035121179472128006 119 44 0.4124520895165702 119 45 0.3494980686179545 119 46 0.8820572684613737 119 47 0.40173639728006805 119 48 0.6009593620453373 119 49 0.44036138530268687 119 50 0.9083433181058993 119 51 0.6428040556721086 119 52 0.6255063001705352 119 53 0.4847002101157152 119 54 0.710681891417007 119 55 0.2322064253530698 119 56 0.22138733625431328 119 57 0.1313938417232201 119 58 0.049548496269938624 120 1 -1.0198566891964163 120 2 -1.9274836590384723 120 3 -1.4587516902431403 120 4 -1.4025446110861708 120 5 -2.2024939526693643 120 6 -1.6567187872076516 120 7 -2.233632298571706 120 8 -2.331923432918863 120 9 -1.8731936499503732 120 10 -1.547249854620695 120 11 -1.4186126004690536 120 12 -1.6774348486111492 120 13 -1.8902163156986589 120 14 -1.627049278699431 120 15 -1.4504316146667975 120 16 -1.2385977429552355 120 17 -0.9626352028814722 120 18 -1.0949661319815078 120 19 -0.8032314283657769 120 20 -0.9159604743091592 120 21 -1.4406909246148274 120 22 -1.160896443755675 120 23 -0.9373485339001917 120 24 -1.1528408849345553 120 25 -1.7000938659485954 120 26 -1.434294893576613 120 27 -1.8670663428561625 120 28 -1.567107507912425 120 29 -1.1776826311242488 120 30 -1.3563531673174012 120 31 -1.3120142216948594 120 32 -1.4335338055988416 120 33 -0.6494723491983834 120 34 -0.3035860254055365 120 35 -0.8654872520323096 120 36 -0.8680873989414692 120 37 -0.7412874289523188 120 38 -0.7873039199998075 120 39 -0.09016313153938427 120 40 -0.7498851041312307 120 41 -0.5043563553401356 120 42 -0.30761573617920135 120 43 -0.19643829240487065 120 44 -0.2782170789059393 120 45 -0.10473979299598568 120 46 0.15230841808995876 120 47 -0.5215345459536564 120 48 -0.2216671739968632 120 49 -0.45290386746180644 120 50 -0.5542764508918054 120 51 -0.5600534826616581 120 52 -0.37489357064043105 120 53 -0.4338491816192658 120 54 0.07937815877678389 120 55 -0.5066471757911764 120 56 -0.6853043479707814 120 57 -1.038059104248081 120 58 -1.1229034807836271 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 275.0 3 43.0 4 65.0 5 73.0 6 116.0 7 236.0 8 240.0 9 352.0 10 421.0 11 477.0 12 698.0 13 820.0 14 961.0 15 1018.0 16 1259.0 17 1308.0 18 1314.0 19 1532.0 20 1696.0 21 1722.0 22 2189.0 23 2581.0 24 3093.0 25 2957.0 26 3390.0 27 3826.0 28 4600.0 29 5838.0 30 7130.0 31 8498.0 32 10955.0 33 13794.0 34 17741.0 35 23589.0 36 32010.0 37 44187.0 38 52929.0 39 47567.0 40 68.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 58.4147585676295 20.8372777601231 5.207646891568683 15.54031678067872 11 30.903630450653747 17.784562288444945 17.917634565606953 33.39417269529435 12 31.025506685059423 10.200684422750426 34.02947262308999 24.74433626910017 13 19.562420415959252 12.56963603565365 40.15313295840407 27.714810589983024 14 16.22856612295112 17.585146915942392 46.0862760182646 20.10001094284189 15 23.601642084040748 12.233725063667231 42.92829477928693 21.236338073005093 16 24.46512892614601 12.941359825976232 35.4155613327674 27.177949915110354 17 27.69988091565578 13.739480606500878 23.21448118699559 35.34615729084775 18 30.228008276740237 14.408690577249574 25.947050084889646 29.41625106112054 19 27.653464558573855 18.526833085738538 31.138913943123942 22.68078841256367 20 32.553180906272274 19.827897799810987 26.3548488717192 21.264072422197536 21 30.986709465195243 17.49423015704584 28.99014484295416 22.528915534804753 22 36.503740946114455 16.70624286965058 25.090870499588764 21.6991456846462 23 39.03630358658744 19.085910971986415 22.32763423174873 19.55015120967742 24 35.58268781833616 21.32454371816638 19.84361735993209 23.249151103565367 25 29.301893675587838 21.13985341425397 20.23413922329453 29.32411368686366 26 30.246860342459847 16.451606482986474 23.864714428824477 29.436818745729205 27 23.417064425287165 18.087043627956653 22.927149637953967 35.568742308802214 28 25.436527289798384 25.38775699787335 22.482109265360155 26.693606446968115 29 31.23018441976661 15.98990754112511 24.328469697724216 28.451438341384062 30 24.828358184167435 16.70115557208956 27.610167971487698 30.86031827225531 31 33.929293934268564 15.228003847046729 25.955294663880874 24.887407554803833 32 42.01650294174223 12.778503724438018 27.757178580667414 17.44781475315234 33 38.95630947208522 12.399380445561794 29.07328851403118 19.571021568321797 34 28.934188618166623 17.10790784681822 35.54716780874168 18.410735726273476 35 25.734462433536 26.22404877288302 34.685666331651625 13.355822461929353 36 29.67953652580166 29.619478657889147 25.72301709492461 14.977967721384584 37 32.395618689256764 23.69637423640629 27.73989534494029 16.16811172939666 38 28.048331270305336 28.08184856157724 26.784629833044065 17.085190335073356 39 32.66465553901765 22.341457580267914 25.806998192642993 19.186888688071445 40 29.054729009926287 23.96480829414478 27.057191500094586 19.923271195834342 41 28.557442444718056 21.86022122733714 28.474473896435338 21.107862431509464 42 27.65342361180258 22.21016296591324 27.26442961930366 22.871983802980516 43 25.510536029636317 18.96352555335732 29.269183275131784 26.256755141874578 44 26.721582752937422 15.439085012494019 30.661850709766604 27.177481524801955 45 28.280310172214623 18.026104391008193 29.65324711537185 24.040338321405336 46 27.547981634014917 20.05384965256682 31.63617288992029 20.76199582349797 47 26.80271340866153 17.971185804742156 33.27644427916366 21.949656507432653 48 29.545907322061808 17.1828722457599 30.78772263025779 22.483497801920496 49 26.43972450802633 17.019001507634478 30.891231146267113 25.65004283807209 50 17.912326201521555 17.088122096440514 34.094886414579314 30.904665287458617 51 17.434017157033686 17.56487025948104 37.83979462040976 27.161317963075515 52 18.535643935996703 17.953342833511048 40.18342650815321 23.327586722339035 53 15.94558600327467 18.542406318187705 40.694256706266046 24.81775097227158 54 17.592063660759358 18.28885139623243 36.48371283577331 27.6353721072349 55 19.63603505438213 16.892013102206512 33.62190965324333 29.85004219016803 56 18.966417029820136 19.892167519287852 28.40807480462842 32.73334064626359 57 21.156120835160745 19.472243265154233 31.23180893903752 28.1398269606475 58 21.245779160352026 22.163396346813432 32.466565632394776 24.124258860439767 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 28.5 9 139.5 10 222.0 11 288.0 12 354.0 13 478.5 14 603.0 15 1091.0 16 1929.0 17 2279.0 18 2335.0 19 2391.0 20 2621.5 21 2852.0 22 3327.0 23 4357.0 24 4912.0 25 5280.5 26 5649.0 27 5990.5 28 5941.5 29 5551.0 30 4706.0 31 3861.0 32 4731.0 33 5601.0 34 4259.0 35 2548.0 36 2179.0 37 2280.5 38 2382.0 39 2679.0 40 2976.0 41 2878.0 42 3265.5 43 3751.0 44 5409.5 45 7068.0 46 6330.5 47 5756.0 48 5919.0 49 6745.5 50 7572.0 51 10386.5 52 13201.0 53 13796.0 54 13714.5 55 13038.0 56 16859.0 57 20680.0 58 25441.5 59 30932.0 60 31661.0 61 35554.5 62 39448.0 63 28976.0 64 18504.0 65 15215.5 66 9928.5 67 7930.0 68 6164.0 69 4398.0 70 3813.5 71 3229.0 72 2532.5 73 1358.5 74 881.0 75 723.0 76 565.0 77 407.0 78 190.5 79 132.0 80 94.0 81 56.0 82 42.5 83 29.0 84 21.5 85 10.5 86 7.0 87 7.0 88 7.0 89 3.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.8697872453310697 11 0.07560483870967742 12 0.0 13 0.0 14 3.316001697792869E-4 15 0.0 16 0.0 17 0.03349161714770798 18 0.0 19 0.0 20 9.948005093378608E-4 21 0.0 22 0.013264006791171477 23 0.0 24 0.0 25 0.012600806451612902 26 0.03382321731748727 27 0.028849214770797965 28 0.05139802631578947 29 0.11705485993208828 30 0.1674580857385399 31 0.012600806451612902 32 0.015253607809847199 33 0.020227610356536505 34 0.023212011884550084 35 0.02951241511035654 36 0.0636672325976231 37 0.06532523344651953 38 0.07659963921901528 39 0.19100169779286927 40 0.08190524193548387 41 0.08256844227504244 42 0.09218484719864177 43 0.10478565365025466 44 0.20691850594227504 45 0.10478565365025466 46 0.119044460950764 47 0.13164526740237692 48 0.13131366723259763 49 0.1439144736842105 50 0.1422564728353141 51 0.154857279286927 52 0.1727636884550085 53 0.15552047962648557 54 0.15651528013582344 55 0.1823800933786078 56 0.24239972410865876 57 0.197633701188455 58 0.22548811544991512 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 301568.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.878833297962647 #Duplication Level Percentage of deduplicated Percentage of total 1 75.19227567837522 22.46657470288625 2 9.33577492924921 5.578841256366723 3 4.110759669274735 3.684741086587436 4 2.3572498751456634 2.8172750424448214 5 1.5681704677875812 2.342755199490662 6 1.1930525498030076 2.1388210950764006 7 0.8856334276677209 1.852318548387097 8 0.7391376727151656 1.7667657045840408 9 0.6270462238499528 1.686186863327674 >10 3.3982575883691246 18.4154154286927 >50 0.29410132623050883 6.125318336162988 >100 0.2707951833971478 16.05375901952462 >500 0.014427612230175907 2.9973339346349746 >1k 0.011098163253981465 7.411926994906621 >5k 0.002219632650796293 4.661966786926995 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 8082 2.679992572156197 No Hit AAGTACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 5977 1.981974214770798 No Hit AAGTACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 4018 1.3323694821731749 No Hit AAGTACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 3494 1.1586109932088287 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 2616 0.8674660441426146 No Hit AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 2404 0.7971668081494058 No Hit AAGTACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 2380 0.7892084040747029 No Hit AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1866 0.6187659168081494 No Hit AAGTACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1845 0.6118023132427844 No Hit AAGTACGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1538 0.5100010611205433 No Hit AAGTACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 1149 0.3810085950764007 No Hit AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1042 0.345527376910017 No Hit AAGTACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 878 0.2911449490662139 No Hit AAGTACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 843 0.2795389431239389 No Hit AAGTACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 815 0.27025413837011886 No Hit AAGTACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC 750 0.2487001273344652 No Hit AAGTACGGGGATTAAACCCCACCCTATCCCCACCTCCAGAGACAGGGTTTCTTTGTGT 744 0.24671052631578946 No Hit AAGTACGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 733 0.24306292444821734 No Hit AAGTACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 662 0.21951931239388794 No Hit AAGTACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 647 0.21454530984719866 No Hit AAGTACGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 646 0.21421370967741934 No Hit AAGTACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 610 0.20227610356536502 No Hit AAGTACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 585 0.19398609932088287 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA 574 0.1903384974533107 No Hit AAGTACGGGAACGCAGAGTGCATGCTAAGTACGGGGCCTTTTCCTGTGGGAGCAGCCG 552 0.18304329371816638 No Hit AAGTACGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 499 0.16546848471986417 No Hit AAGTACGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC 498 0.16513688455008488 No Hit AAGTACGGGATCTATGGGCGGCCCCCACTTACTCGTCTGAAGAGCAAATCACTATATG 497 0.1648052843803056 No Hit AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 492 0.16314728353140917 No Hit AAGTACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG 481 0.15949968166383702 No Hit AAGTACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTT 465 0.15419407894736842 No Hit AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC 457 0.15154127758913413 No Hit AAGTACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 456 0.15120967741935484 No Hit AAGTACGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA 434 0.1439144736842105 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 427 0.1415932724957555 No Hit AAGTACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC 419 0.13894047113752123 No Hit AAGTACGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC 414 0.13728247028862478 No Hit AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 413 0.1369508701188455 No Hit AAGTACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA 412 0.13661926994906623 No Hit AAGTACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 409 0.13562446943972836 No Hit AAGTACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 401 0.13297166808149405 No Hit AAGTACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 399 0.13230846774193547 No Hit AAGTACGGGATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTG 391 0.12965566638370118 No Hit AAGTACGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 390 0.1293240662139219 No Hit AAGTACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 381 0.12633966468590832 No Hit AAGTACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 368 0.12202886247877759 No Hit AAGTACGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC 363 0.12037086162988116 No Hit AAGTACGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA 356 0.11804966044142616 No Hit AAGTACGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG 351 0.1163916595925297 No Hit AAGTACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA 338 0.11208085738539898 No Hit AAGTACGGGCTTCTTTTTACCTCGATCTTGCTCCCTTGCTTCCCAAACAGACGTTGTT 337 0.11174925721561968 No Hit AAGTACGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT 329 0.10909645585738541 No Hit AAGTACGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA 321 0.10644365449915111 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 321 0.10644365449915111 No Hit AAGTACGGGGGGAGAAGCGGGGGCCTGTGCTGGCTTTGTTGCTAAGCTGTGACATCCT 318 0.10544885398981324 No Hit AAGTACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 317 0.10511725382003395 No Hit AAGTACGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 317 0.10511725382003395 No Hit AAGTACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG 312 0.10345925297113752 No Hit AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG 312 0.10345925297113752 No Hit AAGTACGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG 308 0.10213285229202038 No Hit AAGTACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA 306 0.1014696519524618 No Hit AAGTACGGGAACCACCACAGCACTGCTTTCCCATGAGTCACAGGGTGTAGCTTTTACT 302 0.10014325127334464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0046424023769100166 0.0 0.0 0.0 0.0 11 0.007958404074702887 0.0 0.0 0.0 0.0 12 0.007958404074702887 0.0 0.0 0.0 0.0 13 0.008290004244482172 0.0 0.0 0.0 0.0 14 0.012600806451612902 0.0 0.0 0.0 0.0 15 0.012600806451612902 0.0 0.0 0.0 0.0 16 0.013264006791171477 0.0 0.0 0.0 0.0 17 0.013595606960950765 0.0 0.0 0.0 0.0 18 0.01392720713073005 0.0 0.0 0.0 0.0 19 0.01392720713073005 0.0 0.0 0.0 0.0 20 0.01823800933786078 0.0 0.0 0.0 0.0 21 0.01923280984719864 0.0 0.0 0.0 0.0 22 0.019896010186757216 0.0 0.0 0.0 0.0 23 0.021885611205432937 0.0 0.0 0.0 0.0 24 0.023543612054329373 0.0 0.0 0.0 0.0 25 0.023543612054329373 0.0 0.0 0.0 0.0 26 0.023543612054329373 0.0 0.0 0.0 0.0 27 0.024206812393887944 0.0 0.0 0.0 0.0 28 0.03979202037351443 0.0 0.0 0.0 0.0 29 0.04111842105263158 0.0 0.0 0.0 0.0 30 0.04874522495755518 0.0 0.0 0.0 0.0 31 0.04940842529711375 0.0 0.0 0.0 0.0 32 0.05040322580645161 0.0 0.0 0.0 0.0 33 0.05040322580645161 0.0 0.0 0.0 0.0 34 0.05172962648556876 0.0 0.0 0.0 0.0 35 0.08024724108658744 0.0 0.0 0.0 0.0 36 0.08290004244482173 0.0 0.0 0.0 0.0 37 0.0835632427843803 0.0 0.0 0.0 0.0 38 0.08455804329371816 0.0 0.0 0.0 0.0 39 0.0862160441426146 0.0 0.0 0.0 0.0 40 0.08787404499151104 0.0 0.0 0.0 0.0 41 0.09251644736842106 0.0 0.0 0.0 0.0 42 0.09516924872665534 0.0 0.0 0.0 0.0 43 0.09682724957555178 0.0 0.0 0.0 0.0 44 0.09848525042444822 0.0 0.0 0.0 0.0 45 0.10014325127334466 0.0 0.0 0.0 0.0 46 0.10213285229202038 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACTA 15 0.0070478944 52.308975 9 GGTACCT 30 7.655726E-7 52.308975 8 GGACTAA 15 0.0070478944 52.308975 8 ATAGCCG 15 0.0070478944 52.308975 10 GGATCTA 100 0.0 52.308975 8 CGGGCCA 80 0.0 52.308975 6 GATGCCA 20 3.3442944E-4 52.308975 9 GACTAAT 15 0.0070478944 52.308975 9 TTGCTAA 50 3.6379788E-12 52.0739 39 GTTGCTA 50 3.6379788E-12 52.0739 38 AACGGGA 15 0.0071982164 52.0306 44 TGTTAGT 15 0.0071982164 52.0306 44 TATACTA 15 0.0071982164 52.0306 44 CCCGACA 15 0.0071982164 52.0306 43 ATTAGAG 20 3.4335835E-4 52.030598 44 TTTAAGG 20 3.4335835E-4 52.030598 44 CAATTAT 25 1.646511E-5 52.030598 44 CATTAGA 20 3.4335835E-4 52.030598 43 TTGAACT 20 3.4335835E-4 52.030598 44 GGACCTT 20 3.4335835E-4 52.030598 44 >>END_MODULE