FastQCFastQC Report
Tue 31 May 2016
SRR212644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284288
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC69532.445759230076542No Hit
AAGTACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC49041.7250112561909048No Hit
AAGTACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC34531.2146133498424134No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC32231.133709477712742No Hit
AAGTACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT28891.016222985141828No Hit
AAGTACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC26910.9465753039171545No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT26410.9289875056280955No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC23330.820646668167492No Hit
AAGTACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA20050.7052707113912652No Hit
AAGTACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA18220.6408993696533093No Hit
AAGTACGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC13090.4604485592075641No Hit
AAGTACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG10640.37426834759117517No Hit
AAGTACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT9190.3232637325529041No Hit
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC9170.32256022062134176No Hit
AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT9160.3222084646555606No Hit
AAGTACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA8920.3137663214768122No Hit
AAGTACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA7760.2729626294461954No Hit
AAGTACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG7290.25643009905447994No Hit
AAGTACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC7160.25185727149932463No Hit
AAGTACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6800.23919405673120214No Hit
AAGTACGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT6550.23040015758667268No Hit
AAGTACGGGGATTAAACCCCACCCTATCCCCACCTCCAGAGACAGGGTTTCTTTGT6480.2279378658262044No Hit
AAGTACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC6390.2247720621341738No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC6240.2194957226474561No Hit
AAGTACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT6110.21492289509230078No Hit
AAGTACGGGAAGGCCTATTGTACTTAAACCAGTTTGTACAGTTGGCACTATGTAGA5690.20014914452949123No Hit
AAGTACGGGAACGCAGAGTGCATGCTAAGTACGGGGCCTTTTCCTGTGGGAGCAGC5330.18748592976136877No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.1864306618640252No Hit
AAGTACGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5260.1850236380009005No Hit
AAGTACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC5220.18361661413777577No Hit
AAGTACGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4940.17376744709590275No Hit
AAGTACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4740.16673232778027916No Hit
AAGTACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC4710.16567705988293563No Hit
AAGTACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4660.16391828005402972No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT4530.15934545249887438No Hit
AAGTACGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC4490.15793842863574967No Hit
AAGTACGGGATCTATGGGCGGCCCCCACTTACTCGTCTGAAGAGCAAATCACTATA4290.15090330932012605No Hit
AAGTACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4290.15090330932012605No Hit
AAGTACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC4260.14984804142278255No Hit
AAGTACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA4250.14949628545700136No Hit
AAGTACGGGGGGAATTCCTGGCGCTGGCTGTTTCGCTACCGTAGCGCAGGCCGCTC4190.14738574966231427No Hit
AAGTACGGGATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAG4050.14246116614137777No Hit
AAGTACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT4010.14105414227825303No Hit
AAGTACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAG3990.14035063034669068No Hit
AAGTACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA3930.1382400945520036No Hit
AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG3920.1378883385862224No Hit
AAGTACGGGCTTCTTTTTACCTCGATCTTGCTCCCTTGCTTCCCAAACAGACGTTG3730.13120497523638003No Hit
AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.12909443944169294No Hit
AAGTACGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3640.1280391715443494No Hit
AAGTACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3630.12768741557856822No Hit
AAGTACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC3620.12733565961278703No Hit
AAGTACGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3590.1262803917154435No Hit
AAGTACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG3540.1245216118865376No Hit
AAGTACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3540.1245216118865376No Hit
AAGTACGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3480.12241107609185051No Hit
AAGTACGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3380.11889351643403871No Hit
AAGTACGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAG3380.11889351643403871No Hit
AAGTACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG3350.1178382485366952No Hit
AAGTACGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA3310.11643122467357046No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.11643122467357046No Hit
AAGTACGGGGCCCCTTCCTGTTTCCTGTCTCCTCTGTTCCCTGTAGCACCGGCGGC3270.11502420081044576No Hit
AAGTACGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC3250.11432068887888339No Hit
AAGTACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG3210.11291366501575865No Hit
AAGTACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3200.11256190904997748No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.11080312922107159No Hit
AAGTACGGGAACCACCACAGCACTGCTTTCCCATGAGTCACAGGGTGTAGCTTTTA3080.10834083746060334No Hit
AAGTACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG2970.10447152183701036No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC2970.10447152183701036No Hit
AAGTACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC2910.10236098604232328No Hit
AAGTACGGGGTCAGGGCTGGAGAGATGGCTCAGCAGTTAAGAATACTGACTGCCCT2850.1002504502476362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAACT252.0154363E-550.28125449
ACCGGCG451.2914825E-1050.2812548
CATCATT204.0644215E-450.2812549
ACGACTC204.0644215E-450.2812550
ACTTGAT150.00824550550.27232447
ATCAGTC204.0679847E-450.27232447
TTATCTC355.0495146E-850.27232447
GGCACTA700.050.24556444
GTTAGTT355.0707968E-850.24556445
CAATTAT301.0103249E-650.24556444
AACGGGA301.0103249E-650.24556444
TAACAAT150.00826287950.24556445
AATTATC301.0103249E-650.24556445
ATAACAA150.00826287950.24556444
GCACTAT700.050.24556445
AATAACA150.00826287950.24556443
TACCGGT150.00826287950.24556443
TCTATCA204.0786888E-450.24556443
CCACACA204.0786888E-450.24556444
GTACCGG150.00827447850.2277442
Produced by FastQC (version 0.11.5)