##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212644_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 284288 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.61571012494372 38.0 38.0 40.0 33.0 40.0 2 37.45285063034669 38.0 38.0 40.0 33.0 40.0 3 37.02441538158487 38.0 36.0 40.0 33.0 40.0 4 36.23396344552003 38.0 35.0 39.0 30.0 40.0 5 37.46144754615038 38.0 38.0 40.0 33.0 40.0 6 37.651198784331385 39.0 38.0 40.0 33.0 40.0 7 37.9447954187303 39.0 38.0 40.0 35.0 40.0 8 37.8582493809095 39.0 38.0 40.0 35.0 40.0 9 37.7939483903647 39.0 38.0 40.0 35.0 40.0 10 36.92610662426835 38.0 36.0 40.0 32.0 40.0 11 37.30682969383161 39.0 37.0 40.0 33.0 40.0 12 37.189955256641156 39.0 36.0 40.0 33.0 40.0 13 37.1770106371004 39.0 36.0 40.0 32.0 40.0 14 37.22806098041423 39.0 36.0 40.0 33.0 40.0 15 37.307607074515985 39.0 37.0 40.0 33.0 40.0 16 37.10764787820801 39.0 36.0 40.0 33.0 40.0 17 36.76347577104908 38.0 36.0 40.0 32.0 40.0 18 36.647318916028816 38.0 36.0 40.0 31.0 40.0 19 36.49648244034219 38.0 35.0 40.0 31.0 40.0 20 36.4078012438091 38.0 35.0 40.0 31.0 40.0 21 36.188854260468254 38.0 35.0 40.0 31.0 40.0 22 36.17368302566412 38.0 35.0 40.0 30.0 40.0 23 35.86797543336335 38.0 35.0 40.0 30.0 40.0 24 35.62965021386763 38.0 35.0 39.0 29.0 40.0 25 35.517183278928414 38.0 35.0 39.0 29.0 40.0 26 34.89044912201711 38.0 34.0 39.0 28.0 40.0 27 34.71449375281405 38.0 34.0 39.0 27.0 40.0 28 34.31451907924358 38.0 33.0 39.0 26.0 40.0 29 34.19192157248987 38.0 33.0 39.0 26.0 40.0 30 33.96061388451148 37.0 33.0 39.0 25.0 40.0 31 32.92749606033318 36.0 32.0 38.0 22.0 40.0 32 32.80020261143629 36.0 32.0 38.0 21.0 40.0 33 32.326619484466455 36.0 31.0 38.0 18.0 40.0 34 32.16804085997298 36.0 31.0 39.0 18.0 40.0 35 31.662560502026114 35.0 30.0 38.0 15.0 40.0 36 31.735219214317876 36.0 31.0 39.0 9.0 40.0 37 31.36718398244034 35.0 30.0 39.0 2.0 40.0 38 31.034261031067086 35.0 30.0 38.0 2.0 40.0 39 31.023620413102208 35.0 30.0 39.0 2.0 40.0 40 30.76472098716794 35.0 30.0 38.0 2.0 40.0 41 30.16893432012607 35.0 29.0 38.0 2.0 40.0 42 29.787947433588474 35.0 28.0 38.0 2.0 40.0 43 29.46786357496623 35.0 27.0 38.0 2.0 40.0 44 29.4549259905448 35.0 28.0 38.0 2.0 40.0 45 28.759040128320578 35.0 26.0 38.0 2.0 40.0 46 27.947679817649707 34.0 24.0 38.0 2.0 40.0 47 27.612561909049976 33.0 23.0 38.0 2.0 40.0 48 27.697700923007655 33.0 24.0 38.0 2.0 39.0 49 27.374813569338137 33.0 23.0 38.0 2.0 39.0 50 26.94243513619991 33.0 22.0 38.0 2.0 39.0 51 26.355625281404773 33.0 15.0 38.0 2.0 39.0 52 26.107887072264745 33.0 2.0 37.0 2.0 39.0 53 25.712924921206664 33.0 2.0 37.0 2.0 39.0 54 25.355829299864926 33.0 2.0 37.0 2.0 39.0 55 24.87406784669068 33.0 2.0 37.0 2.0 39.0 56 14.322018516434039 18.0 2.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.3444571741325149 1 2 -1.1456201686190752 1 3 -1.7509213917617572 1 4 -2.886247950189073 1 5 -1.141536624165127 1 6 -1.984079992918815 1 7 -1.654181280189654 1 8 -1.74304621389647 1 9 -1.9933395557807998 1 10 -2.3000921959026783 1 11 -1.4977633930487215 1 12 -2.047687001439712 1 13 -1.761467797478268 1 14 -1.38799096277085 1 15 -1.915296479408454 1 16 -1.7156662422414897 1 17 -1.3439205513068586 1 18 -1.6406422184645564 1 19 -1.4484302847733588 1 20 -1.5361878669574338 1 21 -1.264551470093899 1 22 -1.4585123638706463 1 23 -1.758792351008701 1 24 -0.9854192139121025 1 25 -1.71409591768154 1 26 -1.4137652217577426 1 27 -1.3400196614741517 1 28 -1.1550954804496953 1 29 -1.0178675231482899 1 30 -1.303883450599372 1 31 -1.4870254932665112 1 32 -0.7113513743364166 1 33 -1.3940646034495714 1 34 -1.1920613768791384 1 35 -1.4168514958705067 1 36 -0.18817923440800755 1 37 -0.4612874638095974 1 38 -0.6743529624761528 1 39 -0.8590513498197048 1 40 0.2359613124585458 1 41 0.036660756816846884 1 42 -0.2995124953766144 1 43 0.5518672904570359 1 44 0.45283595132215027 1 45 0.2799544326686174 1 46 -0.5034635095692686 1 47 -0.798815056666708 1 48 -0.45750809603409337 1 49 -1.2924123360343103 1 50 -1.1345821290536264 1 51 -1.2629660220968546 1 52 -1.037263329688102 1 53 -1.5525588271567479 1 54 -0.9447995087937713 1 55 -1.8758569165464287 1 56 -0.5662326973139837 2 1 -0.661044450516556 2 2 -0.3928097526139993 2 3 -1.0299306010345433 2 4 -0.4778643363434085 2 5 -0.13171339986111263 2 6 -0.557595394633644 2 7 -0.265168895305564 2 8 -0.2359434299438945 2 9 -0.2749315955817977 2 10 -0.16553095953133834 2 11 -0.4657744018345795 2 12 -0.7816977985181524 2 13 -0.6995857185114005 2 14 0.03627070760069273 2 15 -0.5562618652452258 2 16 -1.0161425839372669 2 17 -0.10193896985242645 2 18 -0.6935479028087954 2 19 -0.19495299039419223 2 20 -0.4291697659729934 2 21 -0.40590851465831435 2 22 0.04187929485699726 2 23 -0.0040342267364437134 2 24 0.6766745724751146 2 25 0.67324397858183 2 26 0.7054683973806064 2 27 1.15700584927 2 28 0.9768829253934328 2 29 0.725437229683294 2 30 0.4546430657550289 2 31 -0.1628167497500499 2 32 -0.12739879679857324 2 33 -0.08658074614037048 2 34 -0.20228684528180452 2 35 -0.20499588033118243 2 36 0.44916595454086305 2 37 0.40370671423634263 2 38 0.02654679406031235 2 39 0.3500520692551312 2 40 0.26096395880130174 2 41 0.21237791570326436 2 42 0.14227854939950646 2 43 0.33725947845322324 2 44 0.17884420791154554 2 45 -0.13190118287070618 2 46 -0.40059487402359295 2 47 -0.24708434850538907 2 48 -0.04211696657500852 2 49 -0.7547390005839034 2 50 -1.0558481394272903 2 51 -0.7287646883401067 2 52 -0.8917356489428911 2 53 -0.8921375293902614 2 54 -0.33607716307555435 2 55 -1.0888980936396848 2 56 -0.4938499302980013 3 1 -0.3531207527909501 3 2 -0.2945522324379155 3 3 -0.7688704396258856 3 4 -0.6700873737608717 3 5 -0.06628786794622243 3 6 -0.15269810923378913 3 7 -0.09422245363278137 3 8 -0.04841591710457038 3 9 -0.15337987144386744 3 10 -0.2402191473523203 3 11 -0.314949016654829 3 12 -0.5241409386502127 3 13 -0.19125849914235715 3 14 0.014994111856012182 3 15 -0.15609678820927542 3 16 -0.4007537358081379 3 17 -0.7332669228971795 3 18 -0.2045897223245703 3 19 -0.5468605472540631 3 20 -0.8454390249605268 3 21 -0.9753509211146607 3 22 -0.5833408078576028 3 23 -0.36586474764619936 3 24 -0.21369593376113016 3 25 -0.9818734095326249 3 26 -0.3322975600662019 3 27 0.013407169886292536 3 28 0.32303112788792987 3 29 0.07530883643311626 3 30 -0.45159317782530906 3 31 0.1382103962514165 3 32 0.24061294201287353 3 33 0.2512218950922005 3 34 0.5450719221429949 3 35 0.35500045129024116 3 36 -0.33421629050682355 3 37 -0.2301157362405455 3 38 -0.1508926776931716 3 39 -0.6336053041858065 3 40 -0.041530538630834 3 41 -0.11621709530187019 3 42 -0.8215145540487718 3 43 -0.14570090305814887 3 44 0.02154413454397286 3 45 -0.7936216524231625 3 46 0.7772664612662119 3 47 0.5792729515679795 3 48 1.0378170026106126 3 49 0.5922162451974629 3 50 0.8287263286578153 3 51 0.7340746366782369 3 52 1.0360339768824929 3 53 0.770738479413847 3 54 1.562748955779913 3 55 1.5177783553698525 3 56 1.222345961117993 4 1 -0.448059335001993 4 2 -0.5092740024541982 4 3 -0.5118591920307978 4 4 -0.7056060950868996 4 5 -0.38871276065008686 4 6 -0.8796664383739454 4 7 -0.49218012715653714 4 8 -0.6136075702050618 4 9 -0.7736440419337285 4 10 -0.7744799139647256 4 11 -1.073810197652314 4 12 -0.7494998257302825 4 13 -0.74412959578288 4 14 -0.3970230555259633 4 15 -1.0531479943658582 4 16 -1.3172292426898906 4 17 -1.874379842832063 4 18 -1.6565284525302815 4 19 -1.7947886218433737 4 20 -1.4608563699361028 4 21 -2.0056528302459284 4 22 -1.36170028691636 4 23 -2.4573809530628097 4 24 -2.133242330090866 4 25 -2.1807967482924298 4 26 -1.5828340407528998 4 27 -1.6192885530676833 4 28 -2.0222404423355442 4 29 -1.9035464247774811 4 30 -2.624746944289715 4 31 -2.0974711192140276 4 32 -2.114870260591836 4 33 -2.3344485207728276 4 34 -1.9594049166908114 4 35 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1.115380198022212 6 44 0.9041843594958756 6 45 0.8337092636638559 6 46 0.5386435516909565 6 47 0.5875791525447447 6 48 0.5889121631848333 6 49 1.1379465989047297 6 50 1.0320525089153243 6 51 0.31380676632156934 6 52 0.09179096963075395 6 53 0.43486457087455577 6 54 0.40935247805310837 6 55 0.8989386335998368 6 56 -0.3074194673265822 7 1 -0.18695781832192893 7 2 -0.4801031322867928 7 3 -0.40317803682329867 7 4 0.010225856078392326 7 5 -0.33869822651195847 7 6 -0.5522550538690467 7 7 -0.38011337271507273 7 8 -0.5627722847374841 7 9 -0.4398718346256203 7 10 -0.38034828597026404 7 11 -0.05789104165826586 7 12 -0.09650664822570576 7 13 -0.18153875741959524 7 14 -0.2399859678252625 7 15 -0.42002383862828907 7 16 -0.24631974762294817 7 17 -0.28588404063313533 7 18 -0.7931664498970363 7 19 -0.2224684604289493 7 20 -0.48469235646210507 7 21 -0.6173690554737732 7 22 -0.15623887077833842 7 23 -0.37439957385859657 7 24 -0.11740866902696467 7 25 -0.6298161214436035 7 26 0.14529208136806204 7 27 0.29251487698891054 7 28 0.14168753673529721 7 29 0.002032974364148288 7 30 0.2155314272028548 7 31 0.8459312370262495 7 32 0.4648195638015764 7 33 0.4593377077038241 7 34 0.986032218887047 7 35 0.19778448755895894 7 36 0.710673117378871 7 37 0.8641966693096705 7 38 1.0221049868211836 7 39 1.010525070865711 7 40 0.47990097668720466 7 41 1.2917201213487672 7 42 1.374151059359665 7 43 1.5188649713751516 7 44 1.8400631618828918 7 45 1.5697009193586737 7 46 1.660200243379137 7 47 1.9090400899111586 7 48 1.8692842371208478 7 49 1.4007751131738324 7 50 1.1399813087374966 7 51 1.2419397280369395 7 52 0.35809057806397604 7 53 1.4204843236232172 7 54 1.1600150637617759 7 55 0.7305136212030696 7 56 -0.39228173499411056 8 1 0.13035625870330136 8 2 -0.08305797956435157 8 3 0.018916916695957298 8 4 0.24988389060694516 8 5 0.12121213205377757 8 6 0.07201453444437078 8 7 0.18163961533273465 8 8 -0.029737756185028275 8 9 0.2709017377515366 8 10 -0.3438111013753087 8 11 -0.01710418906862543 8 12 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-0.2453839355954912 120 40 -0.3530367026472696 120 41 -0.48853708815518715 120 42 -1.0255970982688893 120 43 -0.9148406207640605 120 44 -0.8569622081396062 120 45 -1.0711140590695827 120 46 -0.6301616252086859 120 47 -0.6874547597059184 120 48 -0.5103784592439524 120 49 -1.0349947606985523 120 50 -0.5890197853318213 120 51 -0.85673293880863 120 52 -0.5684416350524693 120 53 -0.6433226247490964 120 54 -0.9040189556011846 120 55 -0.8273172315517137 120 56 -0.06792114626095902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 175.0 3 56.0 4 57.0 5 68.0 6 111.0 7 334.0 8 290.0 9 470.0 10 632.0 11 642.0 12 945.0 13 1227.0 14 1427.0 15 1673.0 16 2022.0 17 2299.0 18 2536.0 19 3010.0 20 3497.0 21 3244.0 22 3482.0 23 4254.0 24 5114.0 25 5618.0 26 5334.0 27 5682.0 28 6555.0 29 7593.0 30 8771.0 31 10338.0 32 12250.0 33 15825.0 34 20635.0 35 26167.0 36 32809.0 37 37439.0 38 34205.0 39 17502.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 57.901494259342634 21.208070688878884 5.393122467357046 15.497312584421433 11 29.947799414678073 17.832620441242682 18.297290072039623 33.92229007203962 12 30.676286019810895 10.130220058532192 34.60469664565511 24.5887972760018 13 19.016631022062132 12.436332170193607 39.43184376407024 29.11519304367402 14 15.958464655560558 17.110817199459703 47.16695745159838 19.76376069338136 15 23.3122748761819 12.072264745610086 43.45241445294912 21.163045925258892 16 23.86101418280054 13.109241332733005 35.18685276902296 27.842891715443496 17 27.182997523638004 13.763859185051777 23.11318099954975 35.939962291760466 18 30.143023975686628 14.360085547050877 26.54667098153985 28.950219495722646 19 28.35082733003152 18.352867514633047 31.27954750112562 22.016757654209815 20 31.528942480864476 19.572053692030618 28.0894726474561 20.809531179648808 21 29.771921431787483 17.314835659612786 29.549259905447993 23.363983003151734 22 35.1805211616389 16.33836109860423 26.080242570914002 22.400875168842862 23 38.46310783430887 19.120750787933364 22.81454018460153 19.601601193156238 24 34.09640927510131 21.760679311121116 21.757161751463304 22.38574966231427 25 28.624264519579967 22.028166436755722 20.822893499619944 28.52467554404437 26 29.65512871956242 16.683209787896068 24.82418533927478 28.83747615326674 27 22.876643340881635 18.343082736688633 23.021739819474625 35.75853410295511 28 25.607329160605406 25.992314384640043 22.486941146220417 25.913415308534134 29 31.44853293497562 16.26243693452465 24.37956255813298 27.909467572366754 30 25.480243911035917 17.278206619435338 27.58027563356949 29.661273835959257 31 33.680087987083944 15.828157276912554 25.632230909693384 24.859523826310113 32 41.179084423347526 12.527551585384348 27.40699466424977 18.88636932701836 33 39.15039252006704 12.387051248125607 28.77198553409191 19.690570697715444 34 29.226990920927737 17.202248420072053 35.324754145215756 18.24600651378445 35 25.57193175641813 25.525344288447016 35.084951541974604 13.817772413160254 36 30.695271756593094 28.268123285301165 25.569447730153634 15.467157227952107 37 31.668915243941203 22.899110693574247 27.882829110481666 17.549144952002884 38 28.52378513009839 26.98945434632845 26.991574075709668 17.49518644786349 39 32.37413884472708 20.906200317965023 27.49832185126303 19.22133898604487 40 30.094372967623357 22.813162731514208 26.953555775484233 20.1389085253782 41 29.45788221654313 20.82966607637085 28.899045276042827 20.813406431043198 42 27.23513242936334 21.00969651088337 28.76902776647717 22.98614329327612 43 26.242619981675976 18.19490677033772 28.803030892606362 26.759442355379942 44 27.317975675847773 14.819479132028215 30.130676873660157 27.73186831846386 45 28.12168188575069 18.388192822255256 29.188079563955544 24.302045728038507 46 27.545927577784855 19.265158755442286 30.39290644578953 22.796007220983327 47 27.2655483669921 17.418632898124244 31.926428771251413 23.389389963632244 48 28.747674809998756 16.866086771661912 30.369018725530143 24.01721969280918 49 26.606807133575742 17.47740220012109 30.076582908167783 25.839207758135384 50 19.401303670114782 17.306220773699874 33.12809975910444 30.16437579708091 51 17.960618149095524 18.16043250596963 37.215778248577564 26.663171096357285 52 20.020131064571807 18.16722251560335 38.56339336029289 23.249253059531952 53 16.420069052058448 19.010230197159935 39.47062451702551 25.099076233756108 54 18.406419994751662 19.104307183840792 35.23691993446669 27.252352886940855 55 19.240859971069575 17.789729699066854 32.61692713503702 30.352483194826558 56 18.891744810593302 19.68269725063372 27.471327530888274 33.95423040788471 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 29.0 9 58.0 10 163.5 11 269.0 12 310.0 13 479.5 14 608.0 15 1127.0 16 1646.0 17 1938.0 18 2230.0 19 2328.0 20 2426.0 21 2664.0 22 3536.5 23 4171.0 24 4586.5 25 5002.0 26 5496.0 27 5990.0 28 6386.0 29 6039.5 30 5297.0 31 4610.0 32 3923.0 33 4884.5 34 5846.0 35 4196.5 36 2547.0 37 2668.0 38 2703.5 39 2618.0 40 2912.5 41 3207.0 42 3675.5 43 4144.0 44 4787.5 45 5431.0 46 5277.5 47 5266.5 48 5409.0 49 6124.0 50 6839.0 51 9169.0 52 11499.0 53 11771.0 54 12408.0 55 12773.0 56 17923.0 57 23073.0 58 23356.0 59 23639.0 60 27018.5 61 30398.0 62 32447.0 63 26806.5 64 19117.0 65 15435.5 66 11754.0 67 9699.0 68 7644.0 69 6033.5 70 4423.0 71 3920.5 72 2694.5 73 1971.0 74 1580.5 75 1190.0 76 867.5 77 545.0 78 445.5 79 270.5 80 195.0 81 141.0 82 87.0 83 63.0 84 39.0 85 29.5 86 20.0 87 14.0 88 4.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.04221071589374156 26 0.0640195857721747 27 0.1192452723998199 28 0.13401902296262946 29 0.16040072039621792 30 0.20331494822152185 31 0.2145711391265196 32 0.25643009905447994 33 0.30356539846915803 34 0.3127110535794687 35 0.33416816749212064 36 0.37813766321476816 37 0.4031123367852319 38 0.43371510580819445 39 0.4354738856371004 40 0.4812021611886538 41 0.4850714768122467 42 0.565975348941918 43 0.5624577892841063 44 0.5649200810445745 45 0.6148694281855019 46 0.6254221071589374 47 0.6665775551553355 48 0.7221549977487618 49 0.6503967807294011 50 0.7063259792886087 51 0.71265758667267 52 0.7517024988743809 53 0.7675315173345341 54 0.806928185502026 55 0.7851193156235929 56 0.7819535119315624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 284288.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.608931787483115 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48692594453111 19.021907361548852 2 10.389181186050815 5.528900270148582 3 4.669116675038997 3.72720621341738 4 2.8183909261560425 2.9997748761819003 5 1.9036036274224677 2.5326429536244937 6 1.4052296221875578 2.243499549752364 7 1.078708722206065 2.009230076542098 8 0.8552996853766226 1.820688878883386 9 0.6649393226343758 1.5923992570914 >10 4.030616291674378 19.422557406573617 >50 0.37543293763054225 6.965119878433139 >100 0.28289665018639454 15.361183025664115 >500 0.023795045342780846 4.36247748761819 >1k 0.014541416598366072 9.966653534443944 >5k 0.001321946963487825 2.445759230076542 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 6953 2.445759230076542 No Hit AAGTACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 4904 1.7250112561909048 No Hit AAGTACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 3453 1.2146133498424134 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 3223 1.133709477712742 No Hit AAGTACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 2889 1.016222985141828 No Hit AAGTACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 2691 0.9465753039171545 No Hit AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 2641 0.9289875056280955 No Hit AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 2333 0.820646668167492 No Hit AAGTACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 2005 0.7052707113912652 No Hit AAGTACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1822 0.6408993696533093 No Hit AAGTACGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1309 0.4604485592075641 No Hit AAGTACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 1064 0.37426834759117517 No Hit AAGTACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 919 0.3232637325529041 No Hit AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 917 0.32256022062134176 No Hit AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 916 0.3222084646555606 No Hit AAGTACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 892 0.3137663214768122 No Hit AAGTACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 776 0.2729626294461954 No Hit AAGTACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 729 0.25643009905447994 No Hit AAGTACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 716 0.25185727149932463 No Hit AAGTACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 680 0.23919405673120214 No Hit AAGTACGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 655 0.23040015758667268 No Hit AAGTACGGGGATTAAACCCCACCCTATCCCCACCTCCAGAGACAGGGTTTCTTTGT 648 0.2279378658262044 No Hit AAGTACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 639 0.2247720621341738 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 624 0.2194957226474561 No Hit AAGTACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 611 0.21492289509230078 No Hit AAGTACGGGAAGGCCTATTGTACTTAAACCAGTTTGTACAGTTGGCACTATGTAGA 569 0.20014914452949123 No Hit AAGTACGGGAACGCAGAGTGCATGCTAAGTACGGGGCCTTTTCCTGTGGGAGCAGC 533 0.18748592976136877 No Hit AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 530 0.1864306618640252 No Hit AAGTACGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 526 0.1850236380009005 No Hit AAGTACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC 522 0.18361661413777577 No Hit AAGTACGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 494 0.17376744709590275 No Hit AAGTACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 474 0.16673232778027916 No Hit AAGTACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 471 0.16567705988293563 No Hit AAGTACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 466 0.16391828005402972 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 453 0.15934545249887438 No Hit AAGTACGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC 449 0.15793842863574967 No Hit AAGTACGGGATCTATGGGCGGCCCCCACTTACTCGTCTGAAGAGCAAATCACTATA 429 0.15090330932012605 No Hit AAGTACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 429 0.15090330932012605 No Hit AAGTACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 426 0.14984804142278255 No Hit AAGTACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 425 0.14949628545700136 No Hit AAGTACGGGGGGAATTCCTGGCGCTGGCTGTTTCGCTACCGTAGCGCAGGCCGCTC 419 0.14738574966231427 No Hit AAGTACGGGATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAG 405 0.14246116614137777 No Hit AAGTACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT 401 0.14105414227825303 No Hit AAGTACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAG 399 0.14035063034669068 No Hit AAGTACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 393 0.1382400945520036 No Hit AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 392 0.1378883385862224 No Hit AAGTACGGGCTTCTTTTTACCTCGATCTTGCTCCCTTGCTTCCCAAACAGACGTTG 373 0.13120497523638003 No Hit AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 0.12909443944169294 No Hit AAGTACGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 364 0.1280391715443494 No Hit AAGTACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 363 0.12768741557856822 No Hit AAGTACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 362 0.12733565961278703 No Hit AAGTACGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 359 0.1262803917154435 No Hit AAGTACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG 354 0.1245216118865376 No Hit AAGTACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 354 0.1245216118865376 No Hit AAGTACGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 348 0.12241107609185051 No Hit AAGTACGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 338 0.11889351643403871 No Hit AAGTACGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAG 338 0.11889351643403871 No Hit AAGTACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG 335 0.1178382485366952 No Hit AAGTACGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA 331 0.11643122467357046 No Hit AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 331 0.11643122467357046 No Hit AAGTACGGGGCCCCTTCCTGTTTCCTGTCTCCTCTGTTCCCTGTAGCACCGGCGGC 327 0.11502420081044576 No Hit AAGTACGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC 325 0.11432068887888339 No Hit AAGTACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 321 0.11291366501575865 No Hit AAGTACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 320 0.11256190904997748 No Hit AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 0.11080312922107159 No Hit AAGTACGGGAACCACCACAGCACTGCTTTCCCATGAGTCACAGGGTGTAGCTTTTA 308 0.10834083746060334 No Hit AAGTACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG 297 0.10447152183701036 No Hit AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC 297 0.10447152183701036 No Hit AAGTACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC 291 0.10236098604232328 No Hit AAGTACGGGGTCAGGGCTGGAGAGATGGCTCAGCAGTTAAGAATACTGACTGCCCT 285 0.1002504502476362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0017587798289058982 0.0 0.0 0.0 0.0 11 0.0028140477262494374 0.0 0.0 0.0 0.0 12 0.0028140477262494374 0.0 0.0 0.0 0.0 13 0.0028140477262494374 0.0 0.0 0.0 0.0 14 0.005979851418280054 0.0 0.0 0.0 0.0 15 0.006331607384061234 0.0 0.0 0.0 0.0 16 0.006331607384061234 0.0 0.0 0.0 0.0 17 0.006331607384061234 0.0 0.0 0.0 0.0 18 0.006331607384061234 0.0 0.0 0.0 0.0 19 0.006683363349842413 0.0 0.0 0.0 0.0 20 0.01020092300765421 0.0 0.0 0.0 0.0 21 0.010552678973435389 0.0 0.0 0.0 0.0 22 0.011607946870778929 0.0 0.0 0.0 0.0 23 0.013366726699684826 0.0 0.0 0.0 0.0 24 0.015125506528590725 0.0 0.0 0.0 0.0 25 0.015477262494371905 0.0 0.0 0.0 0.0 26 0.015829018460153083 0.0 0.0 0.0 0.0 27 0.016532530391715445 0.0 0.0 0.0 0.0 28 0.03271330481764971 0.0 0.0 0.0 0.0 29 0.03376857271499325 0.0 0.0 0.0 0.0 30 0.04537651958577218 0.0 0.0 0.0 0.0 31 0.04608003151733454 0.0 0.0 0.0 0.0 32 0.047487055380459255 0.0 0.0 0.0 0.0 33 0.047838811346240434 0.0 0.0 0.0 0.0 34 0.04889407924358397 0.0 0.0 0.0 0.0 35 0.07633104457451598 0.0 0.0 0.0 0.0 36 0.07668280054029716 0.0 0.0 0.0 0.0 37 0.07879333633498424 0.0 0.0 0.0 0.0 38 0.07984860423232779 0.0 0.0 0.0 0.0 39 0.0812556280954525 0.0 0.0 0.0 0.0 40 0.08442143178748311 0.0 0.0 0.0 0.0 41 0.08723547951373255 0.0 0.0 0.0 0.0 42 0.08829074741107609 0.0 0.0 0.0 0.0 43 0.08934601530841962 0.0 0.0 0.0 0.0 44 0.09040128320576317 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAACT 25 2.0154363E-5 50.281254 49 ACCGGCG 45 1.2914825E-10 50.28125 48 CATCATT 20 4.0644215E-4 50.28125 49 ACGACTC 20 4.0644215E-4 50.28125 50 ACTTGAT 15 0.008245505 50.272324 47 ATCAGTC 20 4.0679847E-4 50.272324 47 TTATCTC 35 5.0495146E-8 50.272324 47 GGCACTA 70 0.0 50.245564 44 GTTAGTT 35 5.0707968E-8 50.245564 45 CAATTAT 30 1.0103249E-6 50.245564 44 AACGGGA 30 1.0103249E-6 50.245564 44 TAACAAT 15 0.008262879 50.245564 45 AATTATC 30 1.0103249E-6 50.245564 45 ATAACAA 15 0.008262879 50.245564 44 GCACTAT 70 0.0 50.245564 45 AATAACA 15 0.008262879 50.245564 43 TACCGGT 15 0.008262879 50.245564 43 TCTATCA 20 4.0786888E-4 50.245564 43 CCACACA 20 4.0786888E-4 50.245564 44 GTACCGG 15 0.008274478 50.22774 42 >>END_MODULE