FastQCFastQC Report
Tue 31 May 2016
SRR212643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269067
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC68792.5566122935923024No Hit
AAGTACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC48751.8118163877398568No Hit
AAGTACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC36011.3383283717438408No Hit
AAGTACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC32931.2238587415030457No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC28901.0740819201165508No Hit
AAGTACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT28211.0484377497054638No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT24090.8953160365262183No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC20940.7782448237799507No Hit
AAGTACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA20200.7507423801506689No Hit
AAGTACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA17100.6355294406225959No Hit
AAGTACGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC13390.49764556783254726No Hit
AAGTACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT13230.49169909353432417No Hit
AAGTACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA10840.4028736337046163No Hit
AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT9090.33783407106780095No Hit
AAGTACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG9090.33783407106780095No Hit
AAGTACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA7710.2865457302456265No Hit
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC7680.2854307663147097No Hit
AAGTACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG7340.27279450843098557No Hit
AAGTACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC6950.25829997732906673No Hit
AAGTACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC6540.24306213693987No Hit
AAGTACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5930.2203912036778944No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC5870.21816127581606065No Hit
AAGTACGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT5840.21704631188514384No Hit
AAGTACGGGGATTAAACCCCACCCTATCCCCACCTCCAGAGACAGGGTTTCTTTGT5810.215931347954227No Hit
AAGTACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA5660.21035652829964283No Hit
AAGTACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT5620.20886990972508704No Hit
AAGTACGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5480.20366674471414184No Hit
AAGTACGGGAAGGCCTATTGTACTTAAACCAGTTTGTACAGTTGGCACTATGTAGA5370.19957854363411343No Hit
AAGTACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC5370.19957854363411343No Hit
AAGTACGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG5090.18917221361222297No Hit
AAGTACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4740.1761643010848599No Hit
AAGTACGGGAACGCAGAGTGCATGCTAAGTACGGGGCCTTTTCCTGTGGGAGCAGC4590.17058948143027572No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.16687293499388628No Hit
AAGTACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4360.16204142462658No Hit
AAGTACGGGGCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTTACCGAG4340.1612981153393021No Hit
AAGTACGGGGGGAATTCCTGGCGCTGGCTGTTTCGCTACCGTAGCGCAGGCCGCTC4240.15758156890291267No Hit
AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG4230.15720991425927372No Hit
AAGTACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC4230.15720991425927372No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT4100.15237840389196744No Hit
AAGTACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4070.1512634399610506No Hit
AAGTACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA4060.15089178531741165No Hit
AAGTACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC3970.14754689352466113No Hit
AAGTACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3970.14754689352466113No Hit
AAGTACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3930.14606027495010535No Hit
AAGTACGGGATCTATGGGCGGCCCCCACTTACTCGTCTGAAGAGCAAATCACTATA3880.14420200173191064No Hit
AAGTACGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3850.1430870378009938No Hit
AAGTACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT3810.141600419226438No Hit
AAGTACGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG3780.1404854552955212No Hit
AAGTACGGGATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAG3750.13937049136460436No Hit
AAGTACGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC3660.13602559957185387No Hit
AAGTACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3640.13528229028457595No Hit
AAGTACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3550.13193739849182545No Hit
AAGTACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG3480.12933581598635283No Hit
AAGTACGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3440.12784919741179707No Hit
AAGTACGGGGCCCCTTCCTGTTTCCTGTCTCCTCTGTTCCCTGTAGCACCGGCGGC3370.12524761490632444No Hit
AAGTACGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG3330.12376099633176867No Hit
AAGTACGGGCTTCTTTTTACCTCGATCTTGCTCCCTTGCTTCCCAAACAGACGTTG3180.1181861766771845No Hit
AAGTACGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAG3180.1181861766771845No Hit
AAGTACGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC3170.11781452203354555No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.11707121274626767No Hit
AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.1140979755971561No Hit
AAGTACGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3030.1126113570226003No Hit
AAGTACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA3010.11186804773532244No Hit
AAGTACGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2950.10963811987348875No Hit
AAGTACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC2920.10852315594257192No Hit
AAGTACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG2890.10740819201165508No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.10740819201165508No Hit
AAGTACGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAG2860.10629322808073825No Hit
AAGTACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA2760.10257668164434881No Hit
AAGTACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC2740.10183337235707092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAAC204.080761E-450.23941849
TCACGGG150.00826640250.23941449
TTTACGG150.00826640250.23941450
AAACACC600.050.23941450
GAGTAAA204.0883129E-450.220648
TACTCCG204.0883129E-450.220648
CTATGTA301.01359E-650.22059648
TAGCTTT252.029591E-550.22059648
GTAGCTT252.0318412E-550.21119747
TCAATCT150.00828478450.21119347
GATAACT204.0958758E-450.20179745
CGATTGT402.575689E-950.20179744
TAGAATC150.00829091950.20179445
TTAGAAT150.00829091950.20179444
ATCCCGC150.00829091950.20179444
GTGTAGC252.0340934E-550.20179445
TCGAGGA150.00829091950.20179444
TCATTAG204.0996613E-450.192442
ATGGACT204.0996613E-450.192442
CATTAGA204.0996613E-450.192443
Produced by FastQC (version 0.11.5)