FastQCFastQC Report
Tue 31 May 2016
SRR212642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24504
Sequences flagged as poor quality0
Sequence length58
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT11834.827783219066275No Hit
GTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9713.962618348024812No Hit
GTTGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4161.6976820111002284No Hit
GTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3431.399771465883121No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2771.1304276852758735No Hit
GTTGCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1700.693764283382305No Hit
GTTGCCGGGGCTGTTGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA1640.6692784851452824No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1090.4448253346392426Illumina Single End Adapter 2 (96% over 25bp)
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.3999347045380346No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT740.30199151158994453Illumina Single End Adapter 2 (96% over 32bp)
GTTGCCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT720.2938295788442703No Hit
GTTGCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA670.2734247469800849No Hit
GTTGCCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT650.2652628142344107No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC630.2571008814887365Illumina Single End Adapter 2 (100% over 31bp)
GTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.24077701599738818No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGCCAA580.23669604962455107No Hit
GTTGCCGGGGCTGTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.22853411687887693No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGGGG540.22037218413320275No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGGAA530.21629121776036564No Hit
GTTGCCGGGGCTGTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.2081292850146915No Hit
GTTGCCGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT470.19180541952334312No Hit
GTTGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.17548155403199478No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGCAA430.17548155403199478No Hit
GTTGCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.17548155403199478No Hit
GTTGCCGGGGGGAATCCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG370.15099575579497226No Hit
GTTGCCGGGGCTGTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAG370.15099575579497226No Hit
GTTGCCGGGATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGA350.14283382304929806No Hit
GTTGCCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG350.14283382304929806No Hit
GTTGCCGGGCGCGGAAAACCTCCGAACTGGCGCAAAGTCTCTCCTCTTGGCCGAAATC330.1346718903036239No Hit
GTTGCCGGGGCTGTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAA320.1305909239307868No Hit
GTTGCCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1305909239307868No Hit
GTTGCCGGGCTGTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGC310.12650995755794972No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGTGG300.12242899118511265No Hit
GTTGCCGGGCTGGGTGACAGAGCGAACTCCATCAAAAGCAAAAACAAACAAACAAACA290.11834802481227553No Hit
GTTGCCGGGGGGAATTCCTTCGATTCCGTTTTGCTACCCGTTGTAGCGCCGGAAGATG280.11426705843943846No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA280.11426705843943846No Hit
GTTGCCGGGGCTGTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11426705843943846No Hit
GTTGCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA270.11018609206660138Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAAGGCGCT260.10610512569376428No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGT260.10610512569376428Illumina Single End Adapter 2 (100% over 24bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGTGCCGGGGAGCCGGGG250.10202415932092719No Hit
GTTGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.10202415932092719No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGGCGACTTTTTTTTTTTTTTTTTTTTTT250.10202415932092719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGGC458.54925E-1152.00000428
AGGGCAG458.54925E-1152.00000430
GAGGGCA458.54925E-1152.00000429
TGATGCT150.00713652952.050
GTTGATG150.00713652952.048
TGGTTGA150.00713652952.046
GGATGAG307.6760625E-752.044
GGTTGAT150.00713652952.047
GTGCAGG150.00713652952.031
GTGCAGC150.00713652952.031
GTGCAGA251.6077356E-552.031
GAGTGCG503.6379788E-1252.029
CGGGGTA251.6077356E-552.06
AACTGGT150.00713652952.043
GGCAACA150.00713652952.034
TCGGCAA150.00713652952.032
AGTGGGA251.6077356E-552.014
TTCTGGT150.00713652952.052
TTTTTAT150.00713652952.049
CAACATT150.00713652952.036
Produced by FastQC (version 0.11.5)