SRR212641_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212641_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24381
Sequences flagged as poor quality	0
Sequence length	58
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	1209	4.958779377384029	No Hit
GTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	928	3.8062425659324886	No Hit
GTTGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	420	1.7226528854435832	No Hit
GTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	329	1.3494114269308066	No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	254	1.0417948402444528	No Hit
GTTGCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	169	0.6931627086665847	No Hit
GTTGCCGGGGCTGTTGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA	144	0.5906238464377999	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	117	0.47988187523071246	Illumina Single End Adapter 2 (96% over 25bp)
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	109	0.4470694393175013	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC	70	0.2871088142405972	Illumina Single End Adapter 2 (100% over 31bp)
GTTGCCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	69	0.2830072597514458	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	67	0.274804150773143	Illumina Single End Adapter 2 (96% over 32bp)
GTTGCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA	60	0.24609326934908332	No Hit
GTTGCCGGGGCTGTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.24609326934908332	No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGCCAA	53	0.21738238792502357	No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGCAA	51	0.2091792789467208	No Hit
GTTGCCGGGGCTGTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50	0.20507772445756942	No Hit
GTTGCCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT	47	0.19277306099011524	No Hit
GTTGCCGGGCGCGGAAAACCTCCGAACTGGCGCAAAGTCTCTCCTCTTGGCCGAAATC	45	0.18456995201181248	No Hit
GTTGCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.18456995201181248	No Hit
GTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43	0.1763668430335097	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG	40	0.16406217956605554	Illumina Single End Adapter 2 (96% over 30bp)
GTTGCCGGGGGGAATTCCTTCGATTCCGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	40	0.16406217956605554	No Hit
GTTGCCGGGGCTGTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40	0.16406217956605554	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGGGG	38	0.15585907058775275	No Hit
GTTGCCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG	37	0.15175751609860139	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCGAACGGAAGAGCTCGTATGCCGTCTTCTGCT	35	0.1435544071202986	Illumina Single End Adapter 1 (96% over 31bp)
GTTGCCGGGGCTGTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAG	35	0.1435544071202986	No Hit
GTTGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.1353512981419958	No Hit
GTTGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.1353512981419958	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGTGG	33	0.1353512981419958	No Hit
GTTGCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	32	0.13124974365284442	Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGGAA	32	0.13124974365284442	No Hit
GTTGCCGGGGCTGTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAA	31	0.12714818916369303	No Hit
GTTGCCGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	31	0.12714818916369303	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGGAGGGCTCGTATGCCGTC	30	0.12304663467454166	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA	27	0.11074197120708748	No Hit
GTTGCCGGGGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAATAA	26	0.1066404167179361	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGTGCCGGGGAGCCGGGG	26	0.1066404167179361	No Hit
GTTGCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC	25	0.10253886222878471	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGATC	15	0.00697361	52.302277	27
CAGAGGG	60	0.0	52.302277	27
CAGGGTC	25	1.5528414E-5	52.302277	27
AGCCATG	15	0.0070310566	52.194214	24
GCAGGGT	25	1.5720669E-5	52.194214	26
CCATGAT	15	0.0070310566	52.194214	26
GAGCCAT	15	0.0070310566	52.194214	23
GCAGAAC	15	0.0070310566	52.194214	21
GCCATGA	15	0.0070310566	52.194214	25
ACGCGGA	15	0.0070310566	52.194214	24
ACGCGAT	25	1.5720669E-5	52.194214	24
CAACGCG	50	3.6379788E-12	52.194214	22
CGCGATC	25	1.5720669E-5	52.194214	25
AACGCGG	20	3.3189423E-4	52.19421	23
AACGCCG	20	3.3189423E-4	52.19421	23
CAACGCC	20	3.3189423E-4	52.19421	22
CGCCTTT	15	0.0070888544	52.086597	44
GCGGTTG	15	0.0070888544	52.086597	38
CTGGTTG	15	0.0070888544	52.086597	45
CTGGTGC	25	1.5914891E-5	52.086597	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content