SRR212640_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212640_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26340
Sequences flagged as poor quality	0
Sequence length	58
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	1269	4.817767653758542	No Hit
GTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	821	3.1169324221716024	No Hit
GTTGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	356	1.351556567957479	No Hit
GTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	279	1.0592255125284737	No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	196	0.7441154138192863	No Hit
GTTGCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	147	0.5580865603644647	No Hit
GTTGCCGGGGCTGTTGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA	125	0.4745634016704632	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	105	0.3986332574031891	Illumina Single End Adapter 2 (96% over 25bp)
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.360668185269552	No Hit
GTTGCCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT	78	0.296127562642369	No Hit
GTTGCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA	67	0.2543659832953683	No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGCCAA	65	0.24677296886864084	No Hit
GTTGCCGGGGCTGTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64	0.24297646165527714	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	64	0.24297646165527714	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC	63	0.23917995444191342	Illumina Single End Adapter 2 (100% over 31bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGGGG	62	0.2353834472285497	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	56	0.21260440394836752	Illumina Single End Adapter 2 (96% over 32bp)
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGCAA	49	0.18602885345482156	No Hit
GTTGCCGGGGCTGTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46	0.17463933181473046	No Hit
GTTGCCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG	41	0.1556567957479119	No Hit
GTTGCCGGGGCTGTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAG	41	0.1556567957479119	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGTGG	40	0.15186028853454822	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTTTT	39	0.1480637813211845	No Hit
GTTGCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.1480637813211845	No Hit
GTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38	0.1442672741078208	No Hit
GTTGCCGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	37	0.1404707668944571	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG	36	0.1366742596810934	Illumina Single End Adapter 2 (96% over 30bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT	33	0.1252847380410023	No Hit
GTTGCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	33	0.1252847380410023	Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33	0.1252847380410023	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGT	33	0.1252847380410023	Illumina Single End Adapter 2 (100% over 24bp)
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGGAA	32	0.12148823082763857	No Hit
GTTGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32	0.12148823082763857	No Hit
GTTGCCGGGCGCGGAAAACCTCCGAACTGGCGCAAAGTCTCTCCTCTTGGCCGAAATC	31	0.11769172361427485	No Hit
GTTGCCGGGGCTGTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.11769172361427485	No Hit
GTTGCCGGGGCTGTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAA	30	0.11389521640091116	No Hit
GTTGCCGGGGGGAATCCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	29	0.11009870918754745	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA	28	0.10630220197418376	No Hit
GTTGCCGGGCTGTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGC	27	0.10250569476082005	No Hit
GTTGCCGGGGGGAATTCCTTCGATTCCGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	27	0.10250569476082005	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAGCAGTGG	27	0.10250569476082005	No Hit
GTTGCCGGGTAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	27	0.10250569476082005	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATGCA	15	0.0070141843	52.23755	8
GGGCATG	30	7.4574746E-7	52.23755	7
CGGGGTT	30	7.4574746E-7	52.23755	6
CGGGGTC	15	0.0070141843	52.23755	6
CGGGGTA	15	0.0070141843	52.23755	6
CGGGGCA	15	0.0070141843	52.23755	6
CGGGCTG	35	3.56049E-8	52.23755	6
CGGGTGA	15	0.0070141843	52.23755	6
CGGGTAG	15	0.0070141843	52.23755	6
CGGGATG	70	0.0	52.23755	6
CGGGATC	55	0.0	52.23755	6
CGGGAGC	15	0.0070141843	52.23755	6
CGGGACG	15	0.0070141843	52.23755	6
GGGATGG	35	3.56049E-8	52.23755	7
GGGATGC	15	0.0070141843	52.23755	7
AACAGTG	15	0.0070141843	52.23755	10
CCGGGAT	140	0.0	52.23755	5
GGAACAT	15	0.0070141843	52.23755	8
GGAACAG	15	0.0070141843	52.23755	8
GGGCTGA	15	0.0070141843	52.23755	8

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content