FastQCFastQC Report
Tue 31 May 2016
SRR212639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33744
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT22506.667852062588904No Hit
GTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8462.507112375533428No Hit
GTTGCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA5961.766239924134661No Hit
GTTGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA4281.2683736367946894No Hit
GTTGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3941.1676149834044571No Hit
GTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.9305357989568516No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.7112375533428165No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGG1410.4178520625889047No Hit
GTTGCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1340.39710763394973925No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG1340.39710763394973925Illumina Single End Adapter 2 (95% over 23bp)
GTTGCCGGGGCTGTTGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCT1160.343764817449028No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG1060.31412991939307733Illumina Single End Adapter 2 (96% over 30bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT990.2933854907539118Illumina Single End Adapter 2 (100% over 29bp)
GTTGCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG900.26671408250355616No Hit
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.24004267425320058No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAGCAGG780.23115220483641538No Hit
GTTGCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGGAAAACATCGAA750.22226173541963018No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGCC740.21929824561403508No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGC710.21040777619724987No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGT640.1896633475580844No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAGCAGT590.17484589853010907No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGG550.1629919393077288No Hit
GTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.15706495969653866No Hit
GTTGCCGGGGCTGTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.14817449027975343No Hit
GTTGCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.14817449027975343No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.14521100047415836No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTT490.14521100047415836No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGGACTTTTTTTTTTTTTTTTTTTT470.13928402086296823No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCCGG470.13928402086296823No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGTGCCGGGGAGCCGG460.13632053105737316No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCC450.13335704125177808Illumina Single End Adapter 2 (100% over 22bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGGAGGGCTCGTATGCCG440.13039355144618303No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTT430.12743006164058795No Hit
GTTGCCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG430.12743006164058795No Hit
GTTGCCGGGGCTGTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.12446657183499288No Hit
GTTGCCGGGATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCA410.12150308202939782No Hit
GTTGCCGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG400.11853959222380275No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC400.11853959222380275Illumina Single End Adapter 2 (96% over 28bp)
GTTGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.11853959222380275No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCC380.11261261261261261Illumina Single End Adapter 2 (100% over 22bp)
GTTGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.11261261261261261No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA380.11261261261261261No Hit
GTTGCCGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGG370.10964912280701754No Hit
GTTGCCGGGGCTGTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.10964912280701754No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGTGCCGGGGAGCAGG340.10075865339023234No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTT340.10075865339023234Illumina Single End Adapter 2 (100% over 27bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG150.00833502750.03863543
GGAGACA301.0121785E-650.03863532
TTGTTCC204.1101157E-450.03863533
GGATGAG252.0348582E-550.03863544
GAGCGCA150.00833502750.03863529
AGCTCGA150.00833502750.03863541
CGGAGAC301.0121785E-650.03863531
GGCATAC150.00833502750.03863549
TCGACGG150.00833502750.03863544
GATATGG252.0348582E-550.03863550
CCAGCCA204.1101157E-450.03863550
TTTCCGG150.00833502750.03863542
GGTCTTG204.1101157E-450.03863529
GCGGCTT150.00833502750.03863549
AGGGTGG252.0348582E-550.03863528
TTTTTAT150.00833502750.03863549
AGGGCAG150.00833502750.03863530
ACTCCGT150.00833502750.03863528
GATGAGA252.0348582E-550.03863545
TCAGCTC150.00833502750.03863539
Produced by FastQC (version 0.11.5)