FastQCFastQC Report
Tue 31 May 2016
SRR212638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30531
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT18976.213356915921523No Hit
GTTGCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA8842.895417772100488No Hit
GTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8072.6432150928564413No Hit
GTTGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA4701.5394189512298975No Hit
GTTGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3771.23481052045462No Hit
GTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.9826078412105729No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.6583472536110838No Hit
GTTGCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1340.4388981690740559No Hit
GTTGCCGGGGCTGTTGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCT1210.39631849595493107No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG1120.36684026071861386Illumina Single End Adapter 2 (95% over 23bp)
GTTGCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG1120.36684026071861386Illumina Single End Adapter 2 (96% over 30bp)
GTTGCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGGAAAACATCGAA1010.33081130654089286No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGG1000.32753594707019096No Hit
GTTGCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG960.3144345091873833No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT920.3013330713045757Illumina Single End Adapter 2 (100% over 29bp)
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.22927516294913367No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGCC690.22599980347843174No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAGCAGG570.18669548983000886No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGC550.18014477088860503No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC540.1768694114179031Illumina Single End Adapter 2 (96% over 28bp)
GTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.16704333300579738No Hit
GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGG500.16376797353509548No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAGCAGT480.15721725459369165No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCCGG470.15394189512298975No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCC460.15066653565228785Illumina Single End Adapter 2 (100% over 22bp)
GTTGCCGGGGCTGTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.15066653565228785No Hit
GTTGCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.15066653565228785No Hit
GTTGCCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG450.14739117618158593No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGGAGGGCTCGTATGCCG440.144115816710884No Hit
GTTGCCGGGGCTGTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.1375650977694802No Hit
GTTGCCGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGG420.1375650977694802No Hit
GTTGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.1342897382987783No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGT400.13101437882807637No Hit
GTTGCCGGGGCTGTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.13101437882807637No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGGGCTCGTATGCCG400.13101437882807637Illumina Single End Adapter 1 (95% over 22bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.12773901935737447No Hit
GTTGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.11791294094526873No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA360.11791294094526873No Hit
GTTGCCGGGCGCGGAAAACCTCCGAACTGGCGCAAAGTCTCTCCTCTTGGCCGAAA360.11791294094526873No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGTGCCGGGGAGCCGG340.11136222200386493No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTT330.10808686253316302No Hit
GTTGCCGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG310.10153614359175919No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCCGTCT310.10153614359175919Illumina Single End Adapter 2 (96% over 26bp)
GTTGCCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.10153614359175919No Hit
GTTGCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA310.10153614359175919Illumina Single End Adapter 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATGG150.00822944450.18811850
GGAGCAG150.00822944450.18811849
CATCGAA1100.050.18811850
CTTTTGC150.00828338150.1054439
TGTTGGC150.00828338150.1054440
CGCCTTT150.00828338150.1054443
GAGTGGA402.4901965E-950.1054429
AGAGGGT150.00828338150.1054428
TGCGATC402.4901965E-950.1054432
GCTGGAA150.00828338150.1054441
GCGGTGG150.00828338150.1054433
AGGGTGG150.00828338150.1054428
TGCTGGA150.00828338150.1054440
CAGAGAG204.0767426E-450.1054427
CAGGGTG150.00828338150.1054427
CAGGGTC150.00828338150.1054427
CAGGGCG150.00828338150.1054434
ATGCTGG150.00828338150.1054439
CCTTTGA150.00828338150.1054445
AGAGAGC204.0767426E-450.1054428
Produced by FastQC (version 0.11.5)