##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212638_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30531 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.68209360977367 39.0 38.0 39.0 35.0 40.0 2 37.44580917755724 39.0 38.0 39.0 34.0 40.0 3 37.73394255019488 39.0 38.0 39.0 35.0 40.0 4 36.74324457109168 38.0 35.0 39.0 32.0 40.0 5 37.00144115816711 39.0 36.0 39.0 33.0 40.0 6 37.16317840883037 39.0 38.0 39.0 33.0 40.0 7 36.92876093151224 38.0 37.0 39.0 33.0 40.0 8 36.74353935344404 39.0 37.0 39.0 32.0 40.0 9 36.55464937276866 39.0 37.0 39.0 31.0 40.0 10 35.40424486587403 38.0 35.0 39.0 29.0 40.0 11 35.10533556057777 38.0 35.0 39.0 27.0 40.0 12 34.930922668762896 38.0 35.0 39.0 27.0 40.0 13 34.811241033703446 38.0 34.0 39.0 27.0 40.0 14 34.35190462153221 38.0 33.0 39.0 26.0 40.0 15 34.78818250302971 38.0 35.0 39.0 27.0 40.0 16 34.37620778880482 38.0 34.0 39.0 26.0 40.0 17 34.93485310012774 38.0 35.0 39.0 28.0 40.0 18 34.70564344436802 38.0 35.0 39.0 27.0 40.0 19 33.58343978251613 37.0 33.0 39.0 23.0 40.0 20 34.10507353182012 38.0 34.0 39.0 25.0 40.0 21 33.675149847695785 38.0 33.0 39.0 23.0 40.0 22 33.39428777308309 38.0 33.0 39.0 22.0 40.0 23 32.77743932396581 37.0 33.0 39.0 19.0 40.0 24 32.85830794929743 37.0 33.0 39.0 19.0 40.0 25 32.81648160885657 38.0 33.0 39.0 17.0 40.0 26 31.827552323867543 36.0 32.0 39.0 2.0 40.0 27 31.766892666470145 37.0 32.0 39.0 2.0 40.0 28 30.648357407225443 35.0 30.0 38.0 2.0 40.0 29 30.95188496937539 36.0 31.0 38.0 2.0 40.0 30 29.408994137106546 35.0 29.0 38.0 2.0 40.0 31 27.96154727981396 35.0 23.0 38.0 2.0 40.0 32 27.01778520192591 34.0 18.0 38.0 2.0 40.0 33 27.441616717434737 35.0 19.0 38.0 2.0 40.0 34 27.3247846451148 35.0 17.0 39.0 2.0 40.0 35 26.6504863908814 35.0 2.0 38.0 2.0 40.0 36 24.85850447086568 34.0 2.0 39.0 2.0 40.0 37 24.458353804330024 33.0 2.0 39.0 2.0 40.0 38 24.45278569322983 34.0 2.0 39.0 2.0 40.0 39 24.040843732599654 33.0 2.0 39.0 2.0 40.0 40 23.675673905211095 33.0 2.0 38.0 2.0 40.0 41 23.619763519046217 33.0 2.0 38.0 2.0 39.0 42 23.558678064917626 33.0 2.0 38.0 2.0 39.0 43 23.33140087124562 33.0 2.0 38.0 2.0 39.0 44 23.125904818053783 32.0 2.0 38.0 2.0 39.0 45 22.923323834790867 32.0 2.0 38.0 2.0 39.0 46 22.81651436245128 33.0 2.0 38.0 2.0 39.0 47 22.82892797484524 33.0 2.0 38.0 2.0 39.0 48 22.75045036192722 33.0 2.0 38.0 2.0 39.0 49 22.490354066358783 32.0 2.0 38.0 2.0 39.0 50 22.14788248010219 32.0 2.0 38.0 2.0 39.0 51 21.643804657561166 32.0 2.0 38.0 2.0 39.0 52 21.20546329959713 30.0 2.0 38.0 2.0 39.0 53 21.090039631849596 30.0 2.0 37.0 2.0 39.0 54 20.932167305361762 31.0 2.0 37.0 2.0 39.0 55 20.380564016900856 30.0 2.0 37.0 2.0 39.0 56 11.531296059742557 2.0 2.0 21.0 2.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.39494766846903673 1 2 -0.762872346327903 1 3 -1.3151103648149203 1 4 -0.5185308237566204 1 5 -1.3123259871119046 1 6 -0.285185968080107 1 7 -0.8809540213310996 1 8 -0.8492024626694246 1 9 -2.025732452512891 1 10 -0.5727237448416886 1 11 -0.3502952102917334 1 12 -0.7880363338463425 1 13 -0.1411944787206707 1 14 -0.9466926301811043 1 15 -2.022114411696407 1 16 -2.2941962119379653 1 17 -2.531905284789012 1 18 -1.2330266256710871 1 19 -3.1477999931612075 1 20 -2.3508510779427105 1 21 -3.8187543432035795 1 22 -4.360161694281093 1 23 -3.8202173808774944 1 24 -3.9726304646916937 1 25 -3.2735869078538187 1 26 -3.8035798366881153 1 27 -4.855178201255107 1 28 -4.3069638926986755 1 29 -5.860273643844458 1 30 -4.433924300187815 1 31 -4.029431887845778 1 32 -4.641234708907959 1 33 -4.386249058459381 1 34 -5.04788323501182 1 35 -5.924852512499481 1 36 -4.9205868509736845 1 37 -4.429949985288566 1 38 -4.32461763088007 1 39 -3.8898677966251647 1 40 -4.150047530334977 1 41 -3.9764996603881357 1 42 -3.717901639537235 1 43 -3.21119984050981 1 44 -3.1150878053710613 1 45 -3.4186603892632377 1 46 -3.376996149286743 1 47 -2.6364429232937816 1 48 -3.418865280123139 1 49 -2.048817442931277 1 50 -1.9343141275559823 1 51 -3.58030384206317 1 52 -2.9388721267091853 1 53 -2.121118255923413 1 54 -1.406522923464319 1 55 -1.556564856728773 1 56 -0.9897744847065315 2 1 -0.1646521019665741 2 2 -0.75178860248554 2 3 -0.29786898550457863 2 4 -1.2537524986334674 2 5 -0.9945919969641182 2 6 -0.31474261832640593 2 7 -0.8513973710848006 2 8 -0.7790054183344495 2 9 -0.009722600296143469 2 10 -1.8067138926249413 2 11 -3.2406892989616836 2 12 -3.1390215555212215 2 13 -1.9921797003955461 2 14 -2.372801004565339 2 15 -2.639109485588037 2 16 -2.054048428686734 2 17 -1.4087525754294106 2 18 -0.4842581527646814 2 19 -2.916272899565147 2 20 -3.7843486148885255 2 21 -1.251020353055793 2 22 -1.9057281967441497 2 23 -1.7093799424538467 2 24 -2.6561280016375086 2 25 -1.3240795186912564 2 26 -0.4944665361955032 2 27 -0.16182844756052717 2 28 -0.11361413900409545 2 29 -0.38120960443559326 2 30 1.011888507693957 2 31 1.799385846144368 2 32 1.207287458579728 2 33 1.1999578380923417 2 34 1.359752232968475 2 35 1.323915960406925 2 36 1.7727629027208955 2 37 2.0183258767803984 2 38 2.434003058775101 2 39 3.1446149619955257 2 40 3.010050991832511 2 41 3.1786727534049675 2 42 3.9458914639110425 2 43 3.2407706028399446 2 44 3.652153573939284 2 45 4.747595768372232 2 46 5.237535870417691 2 47 5.260108800844151 2 48 5.3003465425369605 2 49 5.704877138349513 2 50 4.376030700030224 2 51 5.802701084045204 2 52 4.404723932404117 2 53 5.335778295800726 2 54 6.030669194762279 2 55 5.32151396100522 2 56 4.507762461106278 3 1 0.7352366633282017 3 2 1.0101691283264742 3 3 0.7332767319592719 3 4 0.611653508040618 3 5 1.0955081142705865 3 6 -0.04555463167457674 3 7 -0.41090793838179707 3 8 -1.1360688221164281 3 9 -0.3356402866142716 3 10 0.2867232597666103 3 11 -1.6878528139783668 3 12 -0.934349697901645 3 13 -1.0778304234211333 3 14 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3.5285238982364504 4 41 2.9877860538975796 4 42 1.5678126461770496 4 43 3.2530858737759054 4 44 2.6706264803432234 4 45 1.509911039308193 4 46 0.5158609935704028 4 47 0.5778427909919337 4 48 -0.5974367086945662 4 49 -0.8345317286455618 4 50 -0.8271712704131247 4 51 -3.83030384206317 4 52 -3.22458641242347 4 53 -3.942546827351986 4 54 -4.013665780607177 4 55 -3.1279934281573425 4 56 -1.954060198992245 5 1 0.06140153788017244 5 2 -0.2562585897141503 5 3 -2.955500001036171E-4 5 4 -0.5066260618518541 5 5 -0.1721143469002655 5 6 0.9687822858881532 5 7 0.23544809507101405 5 8 0.40873404526708157 5 9 -0.2651504419308779 5 10 0.5304508583329124 5 11 1.198646588650071 5 12 1.2926514968414864 5 13 1.291345203819013 5 14 1.5109793274908583 5 15 0.45539881581682096 5 16 1.4108302430884905 5 17 1.298782545898817 5 18 0.9627405700961091 5 19 1.8627820174208054 5 20 1.2629055358138999 5 21 1.830716556267319 5 22 2.225817141697739 5 23 1.394068333408221 5 24 0.5763113342501072 5 25 2.336201451934542 5 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117 11 0.2282762182796958 117 12 0.43339223758222545 117 13 0.6469007593745673 117 14 1.362831179342706 117 15 0.25169511211311146 117 16 1.17008950234775 117 17 -0.4604767133604426 117 18 0.5812590886146296 117 19 1.6998190544578407 117 20 -1.5556129827046163 117 21 -1.1544686289178614 117 22 0.11840973429033141 117 23 -0.13926499992511054 117 24 -0.4607257027869309 117 25 -1.6045392888062011 117 26 0.09403921093093359 117 27 -0.7480353441122496 117 28 1.0001789644441814 117 29 0.032583499012684314 117 30 2.194647128383611 117 31 1.8062823978685074 117 32 3.7659081482348995 117 33 0.9470842748739514 117 34 3.0402120030834183 117 35 2.5560998684529004 117 36 1.853222672835841 117 37 0.6700500147114354 117 38 1.0753823691199287 117 39 1.010132203374834 117 40 1.1261429458554986 117 41 1.4235003396118628 117 42 1.398765027129432 117 43 2.2959430166330463 117 44 1.9349121946289394 117 45 1.8361015154986688 117 46 2.2110990888084956 117 47 2.4968904100395513 117 48 1.7049442436863842 117 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118 31 1.9729490645351717 118 32 2.2659081482348995 118 33 2.0804176082072843 118 34 2.1402120030834197 118 35 2.356099868452901 118 36 2.986556006169174 118 37 3.070050014711434 118 38 3.0753823691199287 118 39 3.1434655367081703 118 40 3.559476279188832 118 41 4.156833672945197 118 42 4.865431693796097 118 43 2.962609683299714 118 44 3.7682455279622715 118 45 3.1361015154986696 118 46 3.1110990888084977 118 47 2.8968904100395534 118 48 3.3049442436863856 118 49 3.241658747544914 118 50 2.5656858724440177 118 51 3.626839015079689 118 52 3.358746920909862 118 53 3.1955484107432532 118 54 3.7053818384404416 118 55 4.174387524223608 118 56 1.738796943864898 119 1 0.24790947438810917 119 2 0.3799847965292358 119 3 0.14917534947079503 119 4 0.6243263191005255 119 5 1.0448168700309566 119 6 0.3576711747770389 119 7 -0.30952544990253017 119 8 0.11508325161629074 119 9 -0.5971610239414602 119 10 -0.5370094591274039 119 11 -2.7074380674345875 119 12 -2.6451791909892037 119 13 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0.4141840103038632 120 33 1.5447854242992385 120 34 0.359752232968475 120 35 1.3583987190276154 120 36 -0.2962026145204817 120 37 -0.7402948128747724 120 38 -0.5315141826042087 120 39 -1.8898677966251647 120 40 -1.4037421116157667 120 41 0.4545348223704835 120 42 0.29071905011793575 120 43 0.44766715456408335 120 44 0.8245673670427323 120 45 0.7131130097515417 120 46 1.4444324221418299 120 47 1.329074318085528 120 48 1.3003465425369605 120 49 2.0152219659357193 120 50 2.3415479414095337 120 51 1.699252808183136 120 52 2.404723932404117 120 53 2.1978472613179676 120 54 2.651358849934695 120 55 3.0801346506603906 120 56 3.059486599037312 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70.0 3 36.0 4 134.0 5 56.0 6 140.0 7 428.0 8 147.0 9 203.0 10 268.0 11 251.0 12 312.0 13 441.0 14 428.0 15 372.0 16 501.0 17 575.0 18 637.0 19 779.0 20 977.0 21 935.0 22 623.0 23 839.0 24 794.0 25 865.0 26 468.0 27 401.0 28 488.0 29 542.0 30 690.0 31 826.0 32 960.0 33 1238.0 34 1495.0 35 1931.0 36 2469.0 37 3434.0 38 3240.0 39 1538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 36.67092463397858 52.78241787036128 4.556025023746356 5.990632471913793 11 27.725917919491668 48.49497232321247 12.416887753430938 11.362222003864925 12 60.207657790442504 18.617143231469655 13.700828665946089 7.474370312141758 13 18.446824538993152 26.458353804330027 13.628770757590647 41.46605089908618 14 13.501031738233271 54.92777832367103 24.00183420130359 7.569355736792113 15 46.69679997379713 15.119059316760014 30.444466280174247 7.739674429268613 16 15.390914152828273 13.05230749074711 51.05302806983067 20.503750286593952 17 45.547148799580754 13.039206052864301 11.719236186171432 29.694408961383512 18 45.612655988994796 15.25007369558809 17.811404801676982 21.325865513740133 19 20.061576758049195 14.110248599783827 55.2291113949756 10.59906324719138 20 21.51256100357014 47.119321345517676 14.886508794340179 16.48160885657201 21 23.713602567881825 18.564737479938422 42.47486161606236 15.24679833611739 22 28.092758180210275 20.13691002587534 9.511643902918346 42.258687890996036 23 19.684910418918477 54.3775179325931 13.101437882807637 12.836133765680783 24 13.926828469424517 60.476237267040055 15.220595460351774 10.37633880318365 25 11.776270520003932 28.0972508928864 16.022805465447753 44.10367312166192 26 44.28852459016394 21.74754098360656 15.22622950819672 18.737704918032787 27 15.937038858829316 22.426627315953436 15.809149040826364 45.82718478439089 28 15.730779323012333 52.84702177906061 18.95171870900026 12.47048018892679 29 41.55017391875041 24.023757957603202 23.357616328673622 11.068451794972765 30 11.47670180277805 52.34295471710505 26.870915837520116 9.30942764259679 31 39.878469370996875 24.526194777467563 26.000985383478405 9.594350468057153 32 20.388923562066815 21.420359360115626 44.016686923102185 14.17403015471537 33 36.29103815439219 17.930263892996813 23.004370830457788 22.77432712215321 34 25.2440266868242 20.573832451441156 24.60643507411181 29.575705787622837 35 19.50481701903791 43.55703153256831 24.913688225429915 12.024463222963865 36 27.76150834128525 26.807278470599847 24.260471850218813 21.170741337896086 37 14.75328947368421 24.07236842105263 42.276315789473685 18.898026315789473 38 28.230531235600026 20.841287604502668 30.840629319992104 20.087551839905206 39 17.445841838414434 23.256732731941792 30.723645222887995 28.573780206755778 40 22.24930820925023 22.470022400843327 42.71972591909343 12.560943470813019 41 31.560564122841704 20.61750362462106 37.18861209964413 10.633320152893107 42 20.484668644906034 23.484998351467194 41.20342894823607 14.826904055390703 43 20.255919794208825 21.17934173207572 45.61044785963986 12.954290614075589 44 30.907232134023676 22.92319361540745 36.84661807868614 9.32295617188273 45 20.80295582753274 27.278725299376504 32.98254874146406 18.935770131626693 46 16.46651270207852 28.848564830089078 30.969976905311782 23.71494556252062 47 24.170600468755158 26.963324860528836 34.34126695936355 14.524807711352459 48 26.88637189182049 25.717399200871778 33.52706138757719 13.86916751973054 49 25.78718067199155 26.39448148392633 34.34220080533368 13.476137038748432 50 19.71998415004623 25.303790780610225 37.61722361643112 17.35900145291243 51 19.219595932919585 34.11461772085039 33.863726396408296 12.802059949821734 52 23.299527600673912 25.810181361699314 40.523933798024515 10.36635723960226 53 15.313707998810585 28.638451118379756 37.711038424686954 18.33680245812271 54 18.14457512884895 31.121977005418263 35.99180652834677 14.74164133738602 55 26.254379586170423 27.14682356052092 33.47656508230317 13.122231771005488 56 22.708794659087154 31.16634167300129 35.264566877086295 10.860296790825263 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 111.5 13 171.5 14 120.0 15 105.0 16 90.0 17 74.5 18 59.0 19 89.5 20 120.0 21 134.0 22 163.5 23 179.0 24 256.0 25 333.0 26 363.5 27 394.0 28 550.5 29 799.0 30 891.0 31 970.5 32 1050.0 33 1184.5 34 1319.0 35 1091.0 36 863.0 37 1812.0 38 1776.0 39 791.0 40 678.5 41 566.0 42 531.5 43 497.0 44 488.0 45 479.0 46 1012.0 47 1452.5 48 1360.0 49 1097.0 50 834.0 51 790.0 52 746.0 53 1074.0 54 1866.5 55 2331.0 56 2371.5 57 2412.0 58 2143.0 59 1874.0 60 1805.0 61 1736.0 62 1604.0 63 1346.5 64 1221.0 65 1038.0 66 855.0 67 692.5 68 530.0 69 432.5 70 335.0 71 243.5 72 107.0 73 62.0 74 50.5 75 39.0 76 27.0 77 15.0 78 14.0 79 8.0 80 3.0 81 2.0 82 1.0 83 2.0 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.03930431364842291 26 0.10153614359175919 27 0.11791294094526873 28 0.1408404572401821 29 0.18669548983000886 30 0.25547803871474895 31 0.2816809144803642 32 0.288231633421768 33 0.33408666601159476 34 0.34063738495299856 35 0.3864924175428253 36 0.45855032589826733 37 0.4290720906619501 38 0.48802856113458454 39 0.5142314369001998 40 0.5731879073728342 41 0.5993907831384495 42 0.6583472536110838 43 0.6845501293766991 44 0.6812747699059971 45 0.7140283646130163 46 0.723854443025122 47 0.7795355540270544 48 0.8122891487340735 49 0.7631587566735449 50 0.8090137892633716 51 0.7828109134977563 52 0.8515934623824964 53 0.8646949002653042 54 0.8614195407946023 55 0.907274573384429 56 0.8974484949723232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 30531.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.85549114015263 #Duplication Level Percentage of deduplicated Percentage of total 1 82.011116725619 42.52726736759359 2 9.171298635674583 9.511643902918346 3 3.309752400202122 5.148865087943402 4 1.6738251642243556 3.471881038944024 5 0.9221829206670036 2.391012413612394 6 0.6632137443153108 2.063476466542203 7 0.3663466397170288 1.3297959451049752 8 0.3600303183425973 1.4935639186400709 9 0.30318342597271347 1.4149552913432248 >10 1.0864072764022232 10.471324227834005 >50 0.056846892369883786 2.10933149913203 >100 0.056846892369883786 6.314893059513281 >500 0.012632642748863063 5.538632864956929 >1k 0.0063163213744315315 6.213356915921523 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1897 6.213356915921523 No Hit GTTGCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 884 2.895417772100488 No Hit GTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 807 2.6432150928564413 No Hit GTTGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 470 1.5394189512298975 No Hit GTTGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 377 1.23481052045462 No Hit GTTGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 300 0.9826078412105729 No Hit GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 201 0.6583472536110838 No Hit GTTGCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 134 0.4388981690740559 No Hit GTTGCCGGGGCTGTTGCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCT 121 0.39631849595493107 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 112 0.36684026071861386 Illumina Single End Adapter 2 (95% over 23bp) GTTGCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 112 0.36684026071861386 Illumina Single End Adapter 2 (96% over 30bp) GTTGCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGGAAAACATCGAA 101 0.33081130654089286 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGG 100 0.32753594707019096 No Hit GTTGCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 96 0.3144345091873833 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT 92 0.3013330713045757 Illumina Single End Adapter 2 (100% over 29bp) GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 70 0.22927516294913367 No Hit GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGCC 69 0.22599980347843174 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAGCAGG 57 0.18669548983000886 No Hit GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGC 55 0.18014477088860503 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC 54 0.1768694114179031 Illumina Single End Adapter 2 (96% over 28bp) GTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.16704333300579738 No Hit GTTGCCGGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGGATGAGATGGGG 50 0.16376797353509548 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAGCAGT 48 0.15721725459369165 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCCGG 47 0.15394189512298975 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCC 46 0.15066653565228785 Illumina Single End Adapter 2 (100% over 22bp) GTTGCCGGGGCTGTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.15066653565228785 No Hit GTTGCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.15066653565228785 No Hit GTTGCCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG 45 0.14739117618158593 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGGAGGGCTCGTATGCCG 44 0.144115816710884 No Hit GTTGCCGGGGCTGTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.1375650977694802 No Hit GTTGCCGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGG 42 0.1375650977694802 No Hit GTTGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.1342897382987783 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAGCAGT 40 0.13101437882807637 No Hit GTTGCCGGGGCTGTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.13101437882807637 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGGGCTCGTATGCCG 40 0.13101437882807637 Illumina Single End Adapter 1 (95% over 22bp) GTTGCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.12773901935737447 No Hit GTTGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.11791294094526873 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA 36 0.11791294094526873 No Hit GTTGCCGGGCGCGGAAAACCTCCGAACTGGCGCAAAGTCTCTCCTCTTGGCCGAAA 36 0.11791294094526873 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGTGCCGGGGAGCCGG 34 0.11136222200386493 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTT 33 0.10808686253316302 No Hit GTTGCCGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 31 0.10153614359175919 No Hit GTTGCCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCCGTCT 31 0.10153614359175919 Illumina Single End Adapter 2 (96% over 26bp) GTTGCCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31 0.10153614359175919 No Hit GTTGCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA 31 0.10153614359175919 Illumina Single End Adapter 2 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.009826078412105728 0.0 0.0 0.0 0.0 11 0.03930431364842291 0.0 0.0 0.0 0.0 12 0.04913039206052864 0.0 0.0 0.0 0.0 13 0.05240575153123055 0.0 0.0 0.0 0.0 14 0.11136222200386492 0.0 0.0 0.0 0.0 15 0.11791294094526875 0.0 0.0 0.0 0.0 16 0.12446365988667256 0.0 0.0 0.0 0.0 17 0.13101437882807637 0.0 0.0 0.0 0.0 18 0.1342897382987783 0.0 0.0 0.0 0.0 19 0.1342897382987783 0.0 0.0 0.0 0.0 20 0.16049261406439358 0.0 0.0 0.0 0.0 21 0.1703186924764993 0.0 0.0 0.0 0.0 22 0.18342013035930693 0.0 0.0 0.0 0.0 23 0.22272444400772984 0.0 0.0 0.0 0.0 24 0.2784055550096623 0.0 0.0 0.0 0.0 25 0.28823163342176805 0.0 0.0 0.0 0.0 26 0.3046084307752776 0.0 0.0 0.0 0.0 27 0.3177098686580852 0.0 0.0 0.0 0.0 28 0.7304051619665258 0.0 0.0 0.0 0.0 29 0.7402312403786315 0.0 0.0 0.0 0.0 30 0.8679702597360061 0.0 0.0 0.0 0.0 31 0.8810716976188137 0.0 0.0 0.0 0.0 32 0.8843470570895156 0.0 0.0 0.0 0.0 33 0.9007238544430252 0.0 0.0 0.0 0.0 34 0.9039992139137271 0.0 0.0 0.0 0.0 35 1.2741148341030428 0.0 0.0 0.0 0.0 36 1.2872162719858504 0.0 0.0 0.0 0.0 37 1.2904916314565524 0.0 0.0 0.0 0.0 38 1.3068684288100618 0.0 0.0 0.0 0.0 39 1.3232452261635714 0.0 0.0 0.0 0.0 40 1.3461727424584848 0.0 0.0 0.0 0.0 41 1.3592741803412924 0.0 0.0 0.0 0.0 42 1.3822016966362058 0.0 0.0 0.0 0.0 43 1.3985784939897155 0.0 0.0 0.0 0.0 44 1.4051292129311193 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATGG 15 0.008229444 50.188118 50 GGAGCAG 15 0.008229444 50.188118 49 CATCGAA 110 0.0 50.188118 50 CTTTTGC 15 0.008283381 50.10544 39 TGTTGGC 15 0.008283381 50.10544 40 CGCCTTT 15 0.008283381 50.10544 43 GAGTGGA 40 2.4901965E-9 50.10544 29 AGAGGGT 15 0.008283381 50.10544 28 TGCGATC 40 2.4901965E-9 50.10544 32 GCTGGAA 15 0.008283381 50.10544 41 GCGGTGG 15 0.008283381 50.10544 33 AGGGTGG 15 0.008283381 50.10544 28 TGCTGGA 15 0.008283381 50.10544 40 CAGAGAG 20 4.0767426E-4 50.10544 27 CAGGGTG 15 0.008283381 50.10544 27 CAGGGTC 15 0.008283381 50.10544 27 CAGGGCG 15 0.008283381 50.10544 34 ATGCTGG 15 0.008283381 50.10544 39 CCTTTGA 15 0.008283381 50.10544 45 AGAGAGC 20 4.0767426E-4 50.10544 28 >>END_MODULE