##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212637_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62791
Sequences flagged as poor quality	0
Sequence length	58
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.90546415887627	39.0	38.0	39.0	35.0	40.0
2	36.08189071682247	38.0	35.0	39.0	30.0	40.0
3	37.63958210571579	39.0	38.0	39.0	35.0	40.0
4	36.33761207816407	38.0	35.0	39.0	31.0	40.0
5	37.451561529518564	39.0	38.0	39.0	35.0	40.0
6	37.38535777420331	38.0	38.0	39.0	35.0	40.0
7	37.70080107021707	39.0	38.0	39.0	35.0	40.0
8	37.672166393272924	39.0	38.0	39.0	35.0	40.0
9	36.581612014460674	39.0	35.0	39.0	32.0	40.0
10	34.742447165995124	36.0	34.0	39.0	27.0	40.0
11	34.777404405089904	37.0	34.0	39.0	27.0	40.0
12	35.144527081906645	38.0	34.0	39.0	28.0	40.0
13	34.979057508241624	38.0	33.0	39.0	27.0	40.0
14	35.29061489703938	38.0	34.0	39.0	29.0	40.0
15	35.24901657880906	38.0	34.0	39.0	29.0	40.0
16	34.648675765635204	38.0	33.0	39.0	27.0	40.0
17	34.15720405790639	36.0	33.0	39.0	26.0	40.0
18	34.120654233887024	36.0	33.0	39.0	26.0	40.0
19	33.8251341752799	36.0	32.0	39.0	25.0	40.0
20	33.896529757449315	36.0	32.0	39.0	25.0	40.0
21	33.66652864263987	36.0	32.0	39.0	24.0	40.0
22	33.279673838607444	36.0	31.0	38.0	23.0	40.0
23	32.835310792948036	36.0	31.0	38.0	23.0	40.0
24	32.64822984185632	36.0	30.0	38.0	23.0	39.0
25	32.707219187463174	36.0	31.0	38.0	23.0	40.0
26	32.06899077893328	35.0	30.0	38.0	19.0	39.0
27	31.78399770667771	35.0	30.0	38.0	19.0	39.0
28	31.33004729977226	35.0	29.0	38.0	17.0	39.0
29	31.4399197337198	35.0	29.0	38.0	18.0	39.0
30	31.216718956538358	35.0	29.0	38.0	16.0	39.0
31	30.43579493876511	35.0	28.0	38.0	11.0	39.0
32	30.353840518545653	35.0	28.0	38.0	10.0	39.0
33	29.608956697615902	34.0	27.0	38.0	7.0	39.0
34	29.180774314790337	33.0	26.0	38.0	4.0	39.0
35	28.86765619276648	33.0	25.0	38.0	2.0	39.0
36	29.310824799732444	35.0	26.0	38.0	2.0	39.0
37	29.10491949483206	34.0	26.0	38.0	2.0	39.0
38	28.934942905830454	34.0	26.0	38.0	2.0	39.0
39	29.03868388781832	34.0	26.0	38.0	2.0	39.0
40	28.722555780286985	34.0	26.0	38.0	2.0	39.0
41	28.389434791610263	34.0	25.0	38.0	2.0	39.0
42	28.382220381901863	34.0	25.0	38.0	2.0	39.0
43	28.145960408338773	34.0	24.0	38.0	2.0	39.0
44	27.96853052189008	33.0	24.0	38.0	2.0	39.0
45	27.483445079708876	33.0	23.0	38.0	2.0	39.0
46	27.38363778248475	34.0	22.0	38.0	2.0	39.0
47	27.528785972511983	34.0	23.0	38.0	2.0	39.0
48	27.320810307209634	33.0	23.0	38.0	2.0	39.0
49	27.155101845805927	33.0	22.0	38.0	2.0	39.0
50	26.781162905511938	33.0	21.0	37.0	2.0	39.0
51	26.629978818620504	33.0	19.0	38.0	2.0	39.0
52	26.41550540682582	33.0	18.0	37.0	2.0	39.0
53	25.85155515917886	33.0	14.0	36.0	2.0	39.0
54	25.682773008870697	33.0	9.0	37.0	2.0	39.0
55	25.538389259607268	33.0	2.0	37.0	2.0	39.0
56	24.585880142058574	33.0	2.0	37.0	2.0	39.0
57	24.269767960376488	32.0	2.0	36.0	2.0	39.0
58	23.825213804526125	32.0	2.0	36.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.3601506260763898
1	2	-3.512177633345779
1	3	-0.2486238707693147
1	4	-3.1326573140904443
1	5	-0.2001206542095062
1	6	-0.18585034749873586
1	7	-1.0101708009829267
1	8	-0.8647580046652692
1	9	-2.4753952551612315
1	10	-2.087934119764192
1	11	-3.32437576771396
1	12	-0.7269966131998373
1	13	-3.8822135076740594
1	14	-1.7691615115733583
1	15	-4.993820486015107
1	16	-3.127583539995758
1	17	-2.506430428873294
1	18	-4.473819816028428
1	19	-2.794807263828382
1	20	-4.345092569161679
1	21	-2.2789295239381637
1	22	-3.43196140862651
1	23	-2.371758451654305
1	24	0.2252825919214061
1	25	-0.6846488591696485
1	26	-0.30352522397466686
1	27	0.3713401146224484
1	28	-2.221864639390958
1	29	-1.3998020419437829
1	30	-2.136719156443764
1	31	-2.298018726771261
1	32	-4.0522367743737036
1	33	-3.3795064330119224
1	34	-3.9469536735328425
1	35	-4.411584527146058
1	36	-1.6682590109352624
1	37	-4.37849840403311
1	38	-6.651348985204358
1	39	-3.706795208611311
1	40	-4.711889984413496
1	41	-4.978342641095367
1	42	-3.6656329041308133
1	43	-2.856834588701677
1	44	-2.95229014311278
1	45	-6.585445611532528
1	46	-6.211746413961361
1	47	-4.913187733515365
1	48	-7.880666643511955
1	49	-9.376334307911762
1	50	-8.929444762784364
1	51	-9.016941985268323
1	52	-8.049667865293182
1	53	-9.024291288682797
1	54	-7.567970371581723
1	55	-7.5249651099534205
1	56	-4.591626737128287
1	57	-3.9083750090932163
1	58	-4.712012580040518
2	1	0.07318270725694731
2	2	-2.695510966679116
2	3	0.38470946256401817
2	4	-1.4493239807571072
2	5	0.6332126791238295
2	6	0.5641496525012641
2	7	0.33982919901707476
2	8	0.3019086620013951
2	9	0.007938078172102792
2	10	0.7453992135691436
2	11	-1.3243757677139598
2	12	-2.0769966131998387
2	13	0.6844531589926035
2	14	-2.8691615115733597
2	15	0.556179513984894
2	16	-3.327583539995757
2	17	-0.6897637622066277
2	18	-2.090486482695095
2	19	-1.8948072638283833
2	20	-2.345092569161679
2	21	-4.995596190604832
2	22	-3.048628075293177
2	23	-2.488425118320972
2	24	0.20861592525473682
2	25	-2.71798219250298
2	26	-4.686858557308
2	27	-1.661993218710883
2	28	-3.55519797272429
2	29	-5.0498020419437815
2	30	-4.053385823110432
2	31	-7.181352060104594
2	32	-5.952236774373702
2	33	-6.562839766345256
2	34	-5.330287006866175
2	35	-5.49491786047939
2	36	-6.518259010935264
2	37	-5.161831737366441
2	38	-4.868015651871026
2	39	-5.190128541944642
2	40	-4.978556651080165
2	41	-4.228342641095367
2	42	-4.065632904130812
2	43	-3.5235012553683447
2	44	-5.402290143112779
2	45	-4.518778944865861
2	46	-1.4117464139613602
2	47	-2.613187733515364
2	48	-3.780666643511953
2	49	-0.8430009745784304
2	50	-4.879444762784363
2	51	-8.950275318601657
2	52	-7.283001198626515
2	53	-6.9076246220161295
2	54	-8.53463703824839
2	55	-7.558298443286754
2	56	-5.291626737128286
2	57	-8.158375009093216
2	58	-8.095345913373853
3	1	-0.8777976848999174
3	2	-5.347471750992838
3	3	-1.0956826942987306
3	4	-5.23853966703162
3	5	-0.49423830126833224
3	6	-0.5976150533810909
3	7	-1.1748766833358673
3	8	-2.24711094584174
3	9	-1.7812776081024069
3	10	-2.32322823741125
3	11	-3.471434591243373
3	12	-2.9152319073174873
3	13	-1.1586840959093578
3	14	-4.192690923338066
3	15	-2.134996956603338
3	16	-4.445230598819286
3	17	-5.488783370049767
3	18	-3.673819816028427
3	19	-4.688924910887206
3	20	-6.874504333867563
3	21	-3.0201059945264
3	22	-4.808431996861803
3	23	-5.836464334007246
3	24	-4.992364466902124
3	25	-4.17876650622847
3	26	-7.221172282798197
3	27	-7.063954003024609
3	28	-6.8100999335086065
3	29	-6.8292138066496655
3	30	-5.5484838623261155
3	31	-8.568606962065378
3	32	-8.893413244961938
3	33	-7.861859374188395
3	34	-5.041071320591666
3	35	-7.117466880087235
3	36	-10.474141363876441
3	37	-9.754968992268402
3	38	-12.274878396969065
3	39	-11.48914814978778
3	40	-11.547184102060555
3	41	-8.566577935213015
3	42	-7.712691727660225
3	43	-9.445069882819325
3	44	-8.475819554877486
3	45	-10.538386788003118
3	46	-10.382334649255478
3	47	-12.642599498221246
3	48	-11.898313702335482
3	49	-11.852804896147056
3	50	-11.247091821607892
3	51	-10.999294926444794
3	52	-10.4202561005873
3	53	-10.08899717103574
3	54	-9.485617430405252
3	55	-10.10731805112989
3	56	-9.997509090069462
3	57	-8.849551479681452
3	58	-8.335541991805226
4	1	-0.17191533195874342
4	2	-2.935707045110483
4	3	-0.2721532825340205
4	4	-3.062069078796327
4	5	-1.7883559483271512
4	6	-1.7740856416163808
4	7	-2.2925237421593962
4	8	-2.188287416429972
4	9	-2.663630549278878
4	10	-2.32322823741125
4	11	-4.530258120655137
4	12	-3.7975848484939547
4	13	-4.276331154732883
4	14	-2.5456320998086532
4	15	-4.899702838956284
4	16	-5.209936481172228
4	17	-9.018195134755647
4	18	-8.732643345440191
4	19	-8.512454322651912
4	20	-10.403916098573443
4	21	-6.373047170996987
4	22	-7.0437261145088605
4	23	-7.718817275183717
4	24	-7.051187996313889
4	25	-8.884648859169648
4	26	-8.985878165151139
4	27	-7.299248120671667
4	28	-6.1630411099791935
4	29	-7.593919689002604
4	30	-11.254366215267293
4	31	-9.921548138535968
4	32	-10.18753089202076
4	33	-11.626565256541335
4	34	-12.688130144121079
4	35	-12.352760997734293
4	36	-12.003553128582322
4	37	-12.22555722756252
4	38	-13.157231338145534
4	39	-14.136206973317192
4	40	-12.311889984413497
4	41	-14.566577935213013
4	42	-13.771515257071988
4	43	-12.739187529878148
4	44	-13.005231319583368
4	45	-13.479563258591353
4	46	-12.147040531608418
4	47	-11.466128909985953
4	48	-12.133607819982542
4	49	-11.088099013794116
4	50	-11.776503586313774
4	51	-9.999294926444794
4	52	-10.77319727705789
4	53	-10.206644229859268
4	54	-10.603264489228781
4	55	-9.10731805112989
4	56	-9.35045026654005
4	57	-8.908375009093216
4	58	-9.100247874158166
5	1	-0.4268172927430527
5	2	-2.6677331889013374
5	3	-0.2264016485470961
5	4	-2.699323980757107
5	5	-2.033453987542842
5	6	-1.7969614586098501
5	7	-1.9657263565384824
5	8	-2.309202449109712
5	9	-2.4087285884945686
5	10	-1.532378564208635
5	11	-4.32437576771396
5	12	-3.215885502088728
5	13	-1.148880174340725
5	14	-5.258050400462249
5	15	-6.360487152681774
5	16	-7.049805762217979
5	17	-5.550874873317738
5	18	-4.840486482695095
5	19	-3.2281405971617154
5	20	-6.45620368027279
5	21	-5.023373968382607
5	22	-3.965294741959841
5	23	-3.9050917849876363
5	24	-2.652495185856374
5	25	-5.662426636947426
5	26	-6.659080779530225
5	27	-3.384215440933108
5	28	-6.4440868616131795
5	29	-7.910913153054892
5	30	-5.636719156443764
5	31	-7.486907615660151
5	32	-8.674458996595924
5	33	-7.979506433011924
5	34	-8.191398117977286
5	35	-7.74491786047939
5	36	-9.101592344268596
5	37	-8.356276181810887
5	38	-7.951348985204358
5	39	-9.10679520861131
5	40	-8.64522331774683
5	41	-8.367231529984256
5	42	-10.787855126353033
5	43	-10.467945699812788
5	44	-9.596734587557222
5	45	-8.40766783375475
5	46	-8.91174641396136
5	47	-9.724298844626475
5	48	-7.669555532400842
5	49	-9.231889863467318
5	50	-7.518333651673252
5	51	-6.7836086519349905
5	52	-6.1163345319598506
5	53	-4.435402399793908
5	54	-4.479081482692834
5	55	-5.280520665508977
5	56	-4.902737848239397
5	57	-4.7417083424265485
5	58	-4.62312369115163
6	1	-0.7013270966646203
6	2	-6.582765868639896
6	3	-0.8603885766516726
6	4	-2.532657314090443
6	5	-0.25894418362127425
6	6	-0.30349740632226485
6	7	-1.5866413892182223
6	8	-0.8941697693711532
6	9	-1.7812776081024069
6	10	-2.205581178587721
6	11	-1.1184934147727859
6	12	-1.7975848484939547
6	13	-2.982213507674061
6	14	-0.8985732762792438
6	15	-3.3702910742503995
6	16	-0.9746423635251702
6	17	-5.488783370049767
6	18	-3.2620551101460755
6	19	-7.865395499122499
6	20	-5.286269039749914
6	21	-9.54951775923228
6	22	-8.984902585097096
6	23	-9.130581981066069
6	24	-7.698246819843302
6	25	-9.943472388581412
6	26	-9.162348753386432
6	27	-11.181601061848138
6	28	-8.457158757038016
6	29	-9.476272630179075
6	30	-13.84260150938494
6	31	-12.862724609124204
6	32	-13.246354421432526
6	33	-12.56774172712957
6	34	-12.511659555885785
6	35	-12.058643350675469
6	36	-12.121200187405853
6	37	-11.578498404033109
6	38	-11.921937220498476
6	39	-11.018559914493663
6	40	-11.48836057264879
6	41	-9.390107346977718
6	42	-10.065632904130812
6	43	-9.974481647525206
6	44	-11.181701907818661
6	45	-11.479563258591353
6	46	-11.794099355137831
6	47	-11.70142302763301
6	48	-11.780666643511953
6	49	-12.029275484382351
6	50	-10.364738880431421
6	51	-10.293412573503616
6	52	-9.479079629999065
6	53	-11.85370305338868
6	54	-11.720911548052312
6	55	-10.930847462894596
6	56	-11.115156148892993
6	57	-10.731904420857923
6	58	-10.982600815334637
7	1	-0.2839601498859139
7	2	-3.0169395381076853
7	3	-0.16290958505503284
7	4	-1.9136096950428225
7	5	-0.4858349399237909
7	6	0.004625842977453942
7	7	-0.600646991459115
7	8	-0.6980913379986049
7	9	-1.837300017065992
7	10	-0.9450769769070462
7	11	-4.300566243904434
7	12	-1.2317585179617438
7	13	-2.601261126721681
7	14	-1.7977329401447903
7	15	-2.5747728669674856
7	16	-2.08948830190052
7	17	-2.225478047920916
7	18	-6.340486482695095
7	19	-4.013854882876004
7	20	-7.106997331066442
7	21	-2.936072381081022
7	22	-3.727199503864604
7	23	-5.881282261178114
7	24	-5.398526931888121
7	25	-4.0751250496458375
7	26	-1.7701918906413354
7	27	-3.400088456806124
7	28	-6.221864639390958
7	29	-4.7283734705152085
7	30	-6.184338204062811
7	31	-6.907542536295072
7	32	-8.285570107707034
7	33	-6.8366492901547815
7	34	-6.17552510210427
7	35	-5.459203574765105
7	36	-7.101592344268596
7	37	-5.5308793564140615
7	38	-5.927539461394833
7	39	-7.130604732420835
7	40	-5.26427093679445
7	41	-5.454533117285841
7	42	-3.637061475559385
7	43	-5.190167922035009
7	44	-5.080861571684206
7	45	-4.661636087723004
7	46	-1.6260321282470755
7	47	-2.779854400182032
7	48	-3.1616190244643327
7	49	-4.819191450768905
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120	5	-6.866787320876174
120	6	-7.296961458609847
120	7	-6.076837467649593
120	8	-5.586980226887491
120	9	-7.408728588494565
120	10	-5.087934119764192
120	11	-6.046597989936181
120	12	-7.54921883542206
120	13	-7.759991285451839
120	14	-7.146939289351138
120	15	-8.304931597126217
120	16	-9.883139095551314
120	17	-11.828652651095517
120	18	-9.562708704917316
120	19	-12.061473930495051
120	20	-12.122870346939457
120	21	-7.078929523938164
120	22	-8.74307251973762
120	23	-9.960647340543193
120	24	-9.430272963634152
120	25	-10.884648859169648
120	26	-10.214636335085778
120	27	-13.495326552044219
120	28	-13.110753528279847
120	29	-16.522024264166003
120	30	-16.58116360088821
120	31	-16.209129837882372
120	32	-16.285570107707038
120	33	-18.64617309967859
120	34	-15.969175895755065
120	35	-15.300473416034947
120	36	-16.657147899824153
120	37	-15.80072062625533
120	38	-15.451348985204358
120	39	-16.051239653055752
120	40	-15.867445539969053
120	41	-14.422787085539811
120	42	-14.065632904130812
120	43	-14.634612366479455
120	44	-14.096734587557224
120	45	-15.18544561153253
120	46	-16.245079747294696
120	47	-14.002076622404253
120	48	-14.113999976845287
120	49	-14.565223196800652
120	50	-12.907222540562142
120	51	-12.339164207490544
120	52	-11.616334531959849
120	53	-11.768735733127242
120	54	-11.256859260470613
120	55	-10.669409554397864
120	56	-10.513848959350508
120	57	-11.352819453537661
120	58	-9.73423480226274
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85.0
3	103.0
4	31.0
5	47.0
6	89.0
7	209.0
8	116.0
9	299.0
10	331.0
11	338.0
12	460.0
13	563.0
14	605.0
15	664.0
16	699.0
17	790.0
18	800.0
19	755.0
20	745.0
21	855.0
22	919.0
23	1085.0
24	964.0
25	1127.0
26	1367.0
27	1596.0
28	1835.0
29	2250.0
30	2594.0
31	2949.0
32	3321.0
33	3850.0
34	4453.0
35	5087.0
36	5783.0
37	6540.0
38	5665.0
39	2818.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	50.053351594973805	24.347438327148797	6.289117867210268	19.310092210667136
11	27.94827284164928	23.861699925148507	19.77990476342151	28.410122469780703
12	31.544329601375992	14.169228074087052	33.11461833702282	21.171823987514134
13	17.510471245879184	20.195569428739788	38.413148381137425	23.880810944243603
14	16.52147600770811	23.563886544249975	40.76698889968308	19.14764854835884
15	21.676673408609513	16.753993406698413	36.89063719322833	24.678695991463744
16	21.41389689605198	17.4181013202529	32.2243633641764	28.94363841951872
17	24.941472504021277	15.628035865012501	24.836361898998263	34.59412973196796
18	28.66812122756446	18.91353856444395	24.26940166584383	28.14893854214776
19	24.401586214584892	18.10609800767626	32.933063655619435	24.559252122119414
20	28.150531127072355	19.193833511171984	28.21901227882977	24.436623082925895
21	32.21850613154961	17.072782290173592	24.468864468864467	26.239847109412327
22	35.91279004952939	19.107833925244062	22.791482855823286	22.187893169403257
23	33.16610925306577	24.661570313744228	22.234432234432234	19.937888198757765
24	29.826405478579392	25.314540531931833	22.36184105749323	22.49721293199554
25	28.834209268991877	23.218665392578437	20.648192387322823	27.298932951106863
26	27.181635709051516	23.842673718418453	20.025806862713864	28.949883709816167
27	29.07515647645288	20.024208061921676	21.83184952778353	29.068785933841916
28	29.12989454232644	25.74154904896932	20.392200592602034	24.736355816102208
29	27.361781430687625	18.314458195951005	25.70045077332314	28.623309600038226
30	25.248147853102843	21.018083326694814	28.72460766350673	25.00916115669561
31	30.958829902491875	20.578675673953224	26.30488815244408	22.157606271110826
32	34.874645599057054	18.325316173425506	27.968207447994647	18.831830779522793
33	32.81675559448913	16.927610097953334	28.269491120490564	21.986143187066975
34	25.66896024464832	22.700050968399594	30.100025484199794	21.530963302752294
35	30.20133159185754	23.713803319422762	30.862349080946768	15.22251600777293
36	30.291877768218463	27.10065959277316	25.422999713220534	17.184462925787848
37	29.566311898540565	22.76304888152444	28.178573704671468	19.492065515263526
38	25.637797785037048	24.982869890845354	28.834355828220858	20.54497649589674
39	28.785826947281212	23.096531616932463	26.53464400082846	21.58299743495786
40	31.221618521056072	22.47414795812686	28.694571469543188	17.60966205127388
41	25.707047370182117	24.580551616449707	31.788849763388093	17.923551249980083
42	29.794083896468187	21.737536656891496	29.58370521484126	18.884674231799057
43	26.423243656763994	19.831378299120235	28.01383399209486	25.73154405202091
44	26.082314887107827	17.968737551586226	31.460029637183514	24.488917924122436
45	29.15869980879541	20.58317399617591	30.80465264499681	19.453473550031866
46	27.99821525321095	22.68381298403289	29.763839755234727	19.55413200752143
47	28.939439830386892	21.804211633801472	28.39106661778068	20.865281918030956
48	26.923935220606985	21.2621142565672	28.905253761795464	22.90869676103035
49	21.2636714390485	22.8675743758171	28.74270590861261	27.126048276521797
50	18.4538613608826	21.427204897646835	34.86065939672215	25.258274344748422
51	21.302303339443693	20.594564437714194	34.959751334980474	23.14338088786164
52	21.53855963273504	22.95565403130679	32.37000669493417	23.13577964102401
53	18.835496317081727	24.160581614106693	34.19374382194446	22.810178246867128
54	19.522655527566084	24.705844839131405	30.206307196836836	25.565192436465672
55	23.811498285623156	21.10836456422933	26.551311697631768	28.52882545251575
56	22.772371989152976	24.82692614452066	28.310735364491947	24.08996650183442
57	23.626329808443785	22.251463387402907	28.814775826594573	25.307430977558738
58	23.48747069050771	22.450672323863905	29.976233391287703	24.085623594340678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	155.5
10	311.0
11	202.5
12	94.0
13	96.5
14	99.0
15	105.5
16	207.5
17	303.0
18	402.5
19	502.0
20	432.5
21	363.0
22	405.5
23	530.5
24	613.0
25	739.0
26	865.0
27	1109.0
28	1320.0
29	1287.0
30	1300.0
31	1313.0
32	1132.5
33	952.0
34	1207.0
35	1342.0
36	1222.0
37	948.5
38	675.0
39	684.0
40	693.0
41	780.5
42	945.5
43	1023.0
44	1093.5
45	1164.0
46	1694.5
47	1990.5
48	1756.0
49	1672.0
50	1588.0
51	1662.5
52	1737.0
53	2773.5
54	3253.5
55	2697.0
56	2818.5
57	2940.0
58	3446.5
59	4549.5
60	5146.0
61	5234.0
62	5322.0
63	5109.5
64	4897.0
65	4187.0
66	2921.0
67	2365.0
68	1952.5
69	1540.0
70	1301.5
71	1063.0
72	997.0
73	792.0
74	653.0
75	522.0
76	391.0
77	315.5
78	191.5
79	143.0
80	113.5
81	84.0
82	63.5
83	43.0
84	36.5
85	22.5
86	15.0
87	13.0
88	11.0
89	10.0
90	9.0
91	6.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.001592584924591104
22	0.0
23	0.001592584924591104
24	0.001592584924591104
25	0.001592584924591104
26	0.027073943718048766
27	0.003185169849182208
28	0.027073943718048766
29	0.01592584924591104
30	0.041407208039368704
31	0.0429997929639598
32	0.014333264321319934
33	0.009555509547546623
34	0.011148094472137727
35	0.014333264321319934
36	0.03981462311477759
37	0.0429997929639598
38	0.05733305728527974
39	0.03822203819018649
40	0.047777547737733114
41	0.047777547737733114
42	0.07485149145578188
43	0.07485149145578188
44	0.050962717586915326
45	0.04937013266232421
46	0.05892564220987084
47	0.09555509547546623
48	0.08759217085251071
49	0.10988835979678616
50	0.10670318994760396
51	0.08918475577710182
52	0.09077734070169292
53	0.10988835979678616
54	0.10988835979678616
55	0.13696230351483493
56	0.1608510773837015
57	0.14970298291156375
58	0.15607332260992818
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	62791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.580369798219486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7048503611971	28.43560382857416
2	10.922600619195046	8.427959420936121
3	4.656346749226007	5.389307384816296
4	2.736842105263158	4.2235352200156075
5	1.7626418988648092	3.4001688140020065
6	1.1929824561403508	2.761542259240974
7	0.8255933952528379	2.2296188944275452
8	0.6150670794633644	1.8983612301125958
9	0.5077399380804953	1.7629915115223518
>10	2.716202270381837	18.90239046997181
>50	0.20227038183694532	5.451418196875348
>100	0.13622291021671826	9.65424981287127
>500	0.00825593395252838	2.5847653326113615
>1k	0.01238390092879257	4.878087624022551
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAAGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	1023	1.629214377856699	No Hit
TAAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	1021	1.6260292080075172	No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	1019	1.622844038158335	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	971	1.5463999617779618	No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	652	1.0383653708333997	No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	474	0.7548852542561832	No Hit
TAAGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	363	0.5781083276265707	No Hit
TAAGGGGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	350	0.5574047236068863	No Hit
TAAGGGGGGGATAGAGTACAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGG	307	0.4889235718494689	No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	304	0.48414581707569554	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	282	0.4491089487346913	No Hit
TAAGGGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	262	0.4172572502428692	No Hit
TAAGGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	230	0.3662945326559539	No Hit
TAAGGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA	224	0.35673902310840727	No Hit
TAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	210	0.3344428341641318	No Hit
TAAGGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	163	0.25959134270834994	No Hit
TAAGGGGGGCAAGATACATGCACAAGGCTCTGGGTTTGAGTTCCAACTCATTAAAAAA	158	0.2516284180853944	No Hit
TAAGGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	154	0.24525807838703	No Hit
TAAGGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	150	0.23888773868866559	No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	150	0.23888773868866559	No Hit
TAAGGGGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGTGA	144	0.22933222914111895	No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC	142	0.22614705929193674	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	139	0.22136930451816342	No Hit
TAAGGGGGGATGGTGTGTGGGATCTGCATGGAGGTGGTCTATGAGAAAGCCAACCCCA	137	0.2181841346689812	No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC	135	0.21499896481979902	No Hit
TAAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA	134	0.21340637989520791	No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC	131	0.2086286251214346	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCT	126	0.2006657004984791	No Hit
TAAGGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	126	0.2006657004984791	No Hit
TAAGGGGGGTCCTTTATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCCTT	125	0.19907311557388796	No Hit
TAAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	125	0.19907311557388796	No Hit
TAAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	123	0.19588794572470578	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCT	123	0.19588794572470578	No Hit
TAAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	122	0.19429536080011467	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTCT	117	0.18633243617715914	No Hit
TAAGGGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	117	0.18633243617715914	No Hit
TAAGGGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG	114	0.18155468140338585	No Hit
TAAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	101	0.1608510773837015	No Hit
TAAGGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	96	0.15288815276074597	No Hit
TAAGGGGGGAACACCCTCCTGTTACAGGCTTTTAGTGGAGGAAGAATGTACAGTATGC	94	0.14970298291156375	No Hit
TAAGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAGC	94	0.14970298291156375	No Hit
TAAGGGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA	93	0.14811039798697265	No Hit
TAAGGGGGGGGAACATGGCGGACCTCAGCTTTTCTGACGGCGATCCTACTGTACGCAC	92	0.14651781306238154	No Hit
TAAGGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG	92	0.14651781306238154	No Hit
TAAGGGGGGACTTCTGGGGGTCTTCCATAGCCTGCTCACAGAAAATGCAACCCCAGCG	91	0.14492522813779046	No Hit
TAAGGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC	91	0.14492522813779046	No Hit
TAAGGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	90	0.14333264321319933	No Hit
TAAGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC	88	0.14014747336401714	No Hit
TAAGGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	86	0.13696230351483493	No Hit
TAAGGGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	85	0.13536971859024383	No Hit
TAAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT	79	0.1258142090426972	No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT	78	0.1242216241181061	No Hit
TAAGGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC	77	0.122629039193515	No Hit
TAAGGGGGGGAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGGCAGGCA	75	0.11944386934433279	No Hit
TAAGGGGGGTGAGCTGTCTCTTCAGCCCCTAACTGTACTCTTATCTGTCTACTTAAAA	74	0.11785128441974169	No Hit
TAAGGGGGGATGTATGTGTCTGCTGTTTATAGGTTATAAACATGCTATACATGTGTGT	73	0.11625869949515057	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	73	0.11625869949515057	No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	73	0.11625869949515057	No Hit
TAAGGGGGGCCCTTTTGCTGCCTTGAGCTTTGGACCTAGGCTCCGCTCTTACCAGTGG	71	0.11307352964596837	No Hit
TAAGGGGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC	71	0.11307352964596837	No Hit
TAAGGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	70	0.11148094472137728	No Hit
TAAGGGGGGGGCACTTCCGGTGCCCTTTCTCCCATCTTTTTTAATCGTATTGGTGGCG	70	0.11148094472137728	No Hit
TAAGGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	69	0.10988835979678616	No Hit
TAAGGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGGT	65	0.10351802009842175	No Hit
TAAGGGGGGGCTTCTTCTTCCTGTGTGCTACGGGATGGAACCCAGAGCCTCACACATC	65	0.10351802009842175	No Hit
TAAGGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65	0.10351802009842175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.006370339698364415	0.0	0.0	0.0	0.0
11	0.017518434170502143	0.0	0.0	0.0	0.0
12	0.017518434170502143	0.0	0.0	0.0	0.0
13	0.017518434170502143	0.0	0.0	0.0	0.0
14	0.023888773868866557	0.0	0.0	0.0	0.0
15	0.023888773868866557	0.0	0.0	0.0	0.0
16	0.027073943718048766	0.0	0.0	0.0	0.0
17	0.02866652864263987	0.0	0.0	0.0	0.0
18	0.03025911356723097	0.0	0.0	0.0	0.0
19	0.03025911356723097	0.0	0.0	0.0	0.0
20	0.036629453265595385	0.0	0.0	0.0	0.0
21	0.03822203819018649	0.0	0.0	0.0	0.0
22	0.04618496281314201	0.0	0.0	0.0	0.0
23	0.05574047236068863	0.0	0.0	0.0	0.0
24	0.06051822713446194	0.0	0.0	0.0	0.0
25	0.06211081205905305	0.0	0.0	0.0	0.0
26	0.06529598190823525	0.0	0.0	0.0	0.0
27	0.06529598190823525	0.0	0.0	0.0	0.0
28	0.08918475577710182	0.0	0.0	0.0	0.0
29	0.09077734070169292	0.0	0.0	0.0	0.0
30	0.10351802009842175	0.0	0.0	0.0	0.0
31	0.10351802009842175	0.0	0.0	0.0	0.0
32	0.10351802009842175	0.0	0.0	0.0	0.0
33	0.10351802009842175	0.0	0.0	0.0	0.0
34	0.10351802009842175	0.0	0.0	0.0	0.0
35	0.11785128441974169	0.0	0.0	0.0	0.0
36	0.11785128441974169	0.0	0.0	0.0	0.0
37	0.11944386934433279	0.0	0.0	0.0	0.0
38	0.11944386934433279	0.0	0.0	0.0	0.0
39	0.12262903919351499	0.0	0.0	0.0	0.0
40	0.12262903919351499	0.0	0.0	0.0	0.0
41	0.1258142090426972	0.0	0.0	0.0	0.0
42	0.1289993788918794	0.0	0.0	0.0	0.0
43	0.1321845487410616	0.0	0.0	0.0	0.0
44	0.13536971859024383	0.0	0.0	0.0	0.0
45	0.13536971859024383	0.0	0.0	0.0	0.0
46	0.13855488843942604	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCT	35	3.7625796E-8	52.073544	39
TCATCAA	35	3.7625796E-8	52.073544	52
AGCCTCA	15	0.0071483715	52.07354	46
TGTTGGA	15	0.0071483715	52.07354	51
TTTTGAA	15	0.0071483715	52.07354	37
ACTAACA	15	0.0071483715	52.07354	40
CCAACCC	15	0.0071483715	52.07354	50
CCGTGCG	15	0.0071483715	52.07354	41
GCCTCAC	15	0.0071483715	52.07354	47
CAGCACC	15	0.0071483715	52.07354	48
GCTAGGG	15	0.0071483715	52.07354	37
TGCCGTG	15	0.0071483715	52.07354	39
TGCAACC	20	3.3987014E-4	52.07354	46
CAGTACG	15	0.0071483715	52.07354	37
GTTCCAA	20	3.3987014E-4	52.07354	40
TGCGAAA	15	0.0071483715	52.07354	44
GCGAAAA	15	0.0071483715	52.07354	45
GAGCTGA	15	0.0071483715	52.07354	46
TATGATT	15	0.0071483715	52.07354	51
GTTTCAT	15	0.0071483715	52.07354	41
>>END_MODULE