FastQCFastQC Report
Tue 31 May 2016
SRR212636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66016
Sequences flagged as poor quality0
Sequence length58
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC11001.6662627241880759No Hit
TAAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10391.5738608822103735No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10101.5299321376635966No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT9601.4541929229277752No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6991.0588342220067861No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4680.7089190499272904No Hit
TAAGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3980.6028841492971401No Hit
TAAGGGGGGGATAGAGTACAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGG3300.49987881725642264No Hit
TAAGGGGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3260.49381968007755694No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC2710.41050654386815316No Hit
TAAGGGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2670.4044474066892874No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.402932622394571No Hit
TAAGGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2140.3241638390693165No Hit
TAAGGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2100.3181047018904508No Hit
TAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1860.28174987881725644No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1750.26508725157537566No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1730.2620576829859428No Hit
TAAGGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1720.2605428986912264No Hit
TAAGGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1620.24539505574406204No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.22873242850218128No Hit
TAAGGGGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGTGA1490.2257028599127484No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1450.2196437227338827No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1420.2150993698497334No Hit
TAAGGGGGGCAAGATACATGCACAAGGCTCTGGGTTTGAGTTCCAACTCATTAAAAAA1420.2150993698497334No Hit
TAAGGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1380.20904023267086766No Hit
TAAGGGGGGTCCTTTATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCCTT1380.20904023267086766No Hit
TAAGGGGGGATGGTGTGTGGGATCTGCATGGAGGTGGTCTATGAGAAAGCCAACCCCA1380.20904023267086766No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCT1370.20752544837615125No Hit
TAAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA1300.19692195831313622No Hit
TAAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1300.19692195831313622No Hit
TAAGGGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1280.19389238972370335No Hit
TAAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.18783325254483763No Hit
TAAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1180.178744546776539No Hit
TAAGGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1150.17420019389238972No Hit
TAAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1150.17420019389238972No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCT1130.17117062530295685No Hit
TAAGGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1070.16208191953465825No Hit
TAAGGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1070.16208191953465825No Hit
TAAGGGGGGACTTCTGGGGGTCTTCCATAGCCTGCTCACAGAAAATGCAACCCCAGCG1040.15753756665050897No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTCT1010.1529932137663597No Hit
TAAGGGGGGAACACCCTCCTGTTACAGGCTTTTAGTGGAGGAAGAATGTACAGTATGC1000.15147842947164325No Hit
TAAGGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1000.15147842947164325No Hit
TAAGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAGC950.1439045079980611No Hit
TAAGGGGGGGGAACATGGCGGACCTCAGCTTTTCTGACGGCGATCCTACTGTACGCAC930.14087493940862822No Hit
TAAGGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC930.14087493940862822No Hit
TAAGGGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA930.14087493940862822No Hit
TAAGGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT920.13936015511391178No Hit
TAAGGGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG910.13784537081919535No Hit
TAAGGGGGGTCCCTGTGAACTAGCTTCTCTTTTCAGTGGTCTCCGAATATCGTTGCAG850.12875666505089675No Hit
TAAGGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.12421231216674747No Hit
TAAGGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC810.12269752787203102No Hit
TAAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT800.12118274357731458No Hit
TAAGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG790.11966795928259816No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT790.11966795928259816No Hit
TAAGGGGGGATGTATGTGTCTGCTGTTTATAGGTTATAAACATGCTATACATGTGTGT780.11815317498788172No Hit
TAAGGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG780.11815317498788172No Hit
TAAGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC780.11815317498788172No Hit
TAAGGGGGGCCCTTTTGCTGCCTTGAGCTTTGGACCTAGGCTCCGCTCTTACCAGTGG760.11512360639844886No Hit
TAAGGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG750.11360882210373242No Hit
TAAGGGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA740.112094037809016No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT730.11057925351429956No Hit
TAAGGGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG710.1075496849248667No Hit
TAAGGGGGGGAGGCATTTTGGCCATCTGCACACTTTCACAGTTTCATAGTTTCGGACC700.10603490063015027No Hit
TAAGGGGGGGCTTCTTCTTCCTGTGTGCTACGGGATGGAACCCAGAGCCTCACACATC690.10452011633543383No Hit
TAAGGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT690.10452011633543383No Hit
TAAGGGGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC670.10149054774600097No Hit
TAAGGGGGGTGAGCTGTCTCTTCAGCCCCTAACTGTACTCTTATCTGTCTACTTAAAA670.10149054774600097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCGG150.007162817852.0500852
TAGTGTG307.8276025E-752.0500822
CCCGGTT150.007162817852.0500826
GAGTTAC251.6287813E-552.0500852
TGCCCCT251.6287813E-552.0500852
TCTGCAT150.007162817852.0500823
ATAGCCT150.007162817852.0500827
TTCCATA150.007162817852.0500823
CCCGAGA307.8276025E-752.0500851
GCAGATG150.007162817852.0500852
GCATGGA150.007162817852.0500826
TCCATAG150.007162817852.0500824
GTTAGAC150.007162817852.0500822
CATAGCC150.007162817852.0500826
TGGAATG251.6287813E-552.0500826
TACCCCT251.6287813E-552.0500824
GCCCGGT150.007162817852.0500825
CTGCATG150.007162817852.0500824
ATTTTTA150.007162817852.0500822
TTAGACT150.007162817852.0500823
Produced by FastQC (version 0.11.5)