SRR212635_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212635_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69580
Sequences flagged as poor quality	0
Sequence length	58
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	1135	1.6312158666283414	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	998	1.4343202069560217	No Hit
TAAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	989	1.421385455590687	No Hit
TAAGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	976	1.402701925840759	No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	680	0.9772923253808565	No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	506	0.7272204656510491	No Hit
TAAGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	400	0.5748778384593274	No Hit
TAAGGGGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	328	0.47139982753664844	No Hit
TAAGGGGGGGATAGAGTACAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGG	311	0.4469675194021271	No Hit
TAAGGGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	294	0.42253521126760557	No Hit
TAAGGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	233	0.3348663409025582	No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	232	0.3334291463064099	No Hit
TAAGGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA	228	0.3276803679218166	No Hit
TAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	200	0.2874389192296637	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	185	0.26588100028743894	No Hit
TAAGGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	169	0.2428858867490658	No Hit
TAAGGGGGGCAAGATACATGCACAAGGCTCTGGGTTTGAGTTCCAACTCATTAAAAAA	154	0.22132796780684103	No Hit
TAAGGGGGGATGGTGTGTGGGATCTGCATGGAGGTGGTCTATGAGAAAGCCAACCCCA	153	0.21989077321069272	No Hit
TAAGGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	150	0.21557918942224774	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	146	0.2098304110376545	No Hit
TAAGGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	144	0.20695602184535788	No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	143	0.20551882724920956	No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC	131	0.18827249209542973	No Hit
TAAGGGGGGTCCTTTATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCCTT	129	0.1853981029031331	No Hit
TAAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA	122	0.17533774073009487	No Hit
TAAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	122	0.17533774073009487	No Hit
TAAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	120	0.1724633515377982	No Hit
TAAGGGGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGTGA	115	0.16527737855705663	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTCT	113	0.16240298936476	No Hit
TAAGGGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA	113	0.16240298936476	No Hit
TAAGGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	113	0.16240298936476	No Hit
TAAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	111	0.15952860017246334	No Hit
TAAGGGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	111	0.15952860017246334	No Hit
TAAGGGGGGAACACCCTCCTGTTACAGGCTTTTAGTGGAGGAAGAATGTACAGTATGC	109	0.1566542109801667	No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC	109	0.1566542109801667	No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC	109	0.1566542109801667	No Hit
TAAGGGGGGACTTCTGGGGGTCTTCCATAGCCTGCTCACAGAAAATGCAACCCCAGCG	108	0.15521701638401839	No Hit
TAAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	107	0.15377982178787006	No Hit
TAAGGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	105	0.15090543259557343	No Hit
TAAGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC	103	0.1480310434032768	No Hit
TAAGGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	103	0.1480310434032768	No Hit
TAAGGGGGGGGAACATGGCGGACCTCAGCTTTTCTGACGGCGATCCTACTGTACGCAC	97	0.1394078758263869	No Hit
TAAGGGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG	93	0.13365909744179363	No Hit
TAAGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAGC	91	0.13078470824949698	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCT	88	0.12647312446105202	No Hit
TAAGGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG	87	0.1250359298649037	No Hit
TAAGGGGGGTCCCTGTGAACTAGCTTCTCTTTTCAGTGGTCTCCGAATATCGTTGCAG	84	0.12072434607645875	No Hit
TAAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT	83	0.11928715148031042	No Hit
TAAGGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	83	0.11928715148031042	No Hit
TAAGGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.11784995688416211	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCT	80	0.11497556769186547	No Hit
TAAGGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC	79	0.11353837309571715	No Hit
TAAGGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	76	0.1092267893072722	No Hit
TAAGGGGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC	75	0.10778959471112387	No Hit
TAAGGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC	75	0.10778959471112387	No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	73	0.10491520551882726	No Hit
TAAGGGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	71	0.10204081632653061	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTTGA	30	7.6441574E-7	52.234615	10
GGACTGT	15	0.007064703	52.234615	8
GGACATG	20	3.3497246E-4	52.234615	8
GACACTA	15	0.007064703	52.234615	10
GAACATT	15	0.007064703	52.234615	9
GGGACTG	55	0.0	52.234615	7
GACTGGT	15	0.007064703	52.234615	9
GGGATGT	15	0.007064703	52.234615	7
GGGATAC	20	3.3497246E-4	52.234615	7
GGGATAA	15	0.007064703	52.234615	7
AGCTGGT	15	0.007064703	52.234615	10
GGTCGAA	15	0.007064703	52.234615	10
GTTTTCC	75	0.0	52.23461	10
GGATAGA	45	8.54925E-11	52.23461	9
GATAGAG	45	8.54925E-11	52.23461	10
AAGCATG	50	3.6379788E-12	52.23461	10
CCCAGCA	25	1.6164888E-5	52.121387	38
GATACGT	15	0.007125648	52.121387	39
AAGACGC	25	1.6164888E-5	52.121387	38
CTTGTCT	45	8.731149E-11	52.121387	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content