FastQCFastQC Report
Tue 31 May 2016
SRR212634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89706
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT15741.7546206496778363No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC13861.5450471540365192No Hit
TAAGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA13271.4792767484895102No Hit
TAAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA12311.3722604953960715No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT10801.2039328473011839No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC8690.9687200410228971No Hit
TAAGGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC7710.8594742826566785No Hit
TAAGGGGGGGATAGAGTACAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCG5160.5752123603772322No Hit
TAAGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4250.4737698704657437No Hit
TAAGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT4250.4737698704657437No Hit
TAAGGGGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGT4200.46819610728379374No Hit
TAAGGGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT3820.42583550710097434No Hit
TAAGGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3790.42249124919180436No Hit
TAAGGGGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3360.37455688582703495No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3200.3567208436447952No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.33554054355338553No Hit
TAAGGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2880.3210487592803157No Hit
TAAGGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2730.30432746973446595No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2580.2876061801886161No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2570.2864914275522262No Hit
TAAGGGGGGGGCACTTCCGGTGCCCTTTCTCCCATCTTTTTTAATCGTATTGGTGG2450.27311439591554637No Hit
TAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2440.2719996432791564No Hit
TAAGGGGGGTCCTTTATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCC2360.26308162218803643No Hit
TAAGGGGGGCAAGATACATGCACAAGGCTCTGGGTTTGAGTTCCAACTCATTAAAA2250.2508193431877466No Hit
TAAGGGGGGAACACCCTCCTGTTACAGGCTTTTAGTGGAGGAAGAATGTACAGTAT2200.24524558000579672No Hit
TAAGGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2150.23967181682384678No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.23521280627828686No Hit
TAAGGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2090.23298330100550688No Hit
TAAGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC1960.21849151673243708No Hit
TAAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.20399973245936726No Hit
TAAGGGGGGATGGTGTGTGGGATCTGCATGGAGGTGGTCTATGAGAAAGCCAACCC1640.18281943236795756No Hit
TAAGGGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT1590.17724566918600762No Hit
TAAGGGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT1500.16721289545849777No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1420.15829487436737788No Hit
TAAGGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1420.15829487436737788No Hit
TAAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1410.1571801217309879No Hit
TAAGGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1350.15049160591264799No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1330.148262100639868No Hit
TAAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1280.1426883374579181No Hit
TAAGGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1280.1426883374579181No Hit
TAAGGGGGGACTTCTGGGGGTCTTCCATAGCCTGCTCACAGAAAATGCAACCCCAG1260.14045883218513813No Hit
TAAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1260.14045883218513813No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1190.1326555637304082No Hit
TAAGGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1120.12485229527567834No Hit
TAAGGGGGGTGAGCTGTCTCTTCAGCCCCTAACTGTACTCTTATCTGTCTACTTAA1120.12485229527567834No Hit
TAAGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC1110.12373754263928835No Hit
TAAGGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.12039328473011839No Hit
TAAGGGGGGGGAACATGGCGGACCTCAGCTTTTCTGACGGCGATCCTACTGTACGC1070.11927853209372839No Hit
TAAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.11593427418455844No Hit
TAAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAA1040.11593427418455844No Hit
TAAGGGGGGTCCCTGTGAACTAGCTTCTCTTTTCAGTGGTCTCCGAATATCGTTGC970.10813100572982855No Hit
TAAGGGGGGTGTTTTTTTGAGCGTTCGGTGGATGCCTTGGCATCAGGGGCCGATGA940.1047867478206586No Hit
TAAGGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.1036719951842686No Hit
TAAGGGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG930.1036719951842686No Hit
TAAGGGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC920.10255724254787862No Hit
TAAGGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG900.10032773727509867No Hit
TAAGGGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA900.10032773727509867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATC150.00821514350.2907845
ACAGTGA150.00821514350.2907843
GTTTGAT150.00821514350.2907848
AATCCAG150.00821514350.2907848
TATACTA150.00821514350.2907844
ACATGTG150.00821514350.2907849
TACAGGT150.00821514350.2907847
TATACAT150.00821514350.2907846
CGCCCTT150.00821514350.2907844
TATAACT150.00821514350.2907843
GCGGCAT150.00821514350.2907842
GTATTGG150.00821514350.2907847
CAATTAT550.050.2907844
ATAACTC150.00821514350.2907844
ACTCATT309.962569E-750.2907846
CAATAAG150.00821514350.2907847
TGCCTAG150.00821514350.2907843
GGCCTTA150.00821514350.2907844
TAACTCT150.00821514350.2907845
CGTATTG150.00821514350.2907846
Produced by FastQC (version 0.11.5)