SRR212633_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212633_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83039
Sequences flagged as poor quality	0
Sequence length	56
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1416	1.705222847095943	No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	1375	1.6558484567492384	No Hit
TAAGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1303	1.5691422102867325	No Hit
TAAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1239	1.4920699912089501	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	1068	1.2861426558604994	No Hit
TAAGGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	885	1.0657642794349644	No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	846	1.0187983959344404	No Hit
TAAGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT	488	0.5876756704680933	No Hit
TAAGGGGGGGATAGAGTACAAGTGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCG	456	0.5491395609292019	No Hit
TAAGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	452	0.5443225472368405	No Hit
TAAGGGGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT	411	0.49494815689013594	No Hit
TAAGGGGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGT	381	0.45882055419742535	No Hit
TAAGGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	362	0.4359397391587086	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	318	0.38295258854273295	No Hit
TAAGGGGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	311	0.3745228145811004	No Hit
TAAGGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	281	0.3383952118883898	No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	277	0.3335781981960284	No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	249	0.2998591023494984	No Hit
TAAGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC	242	0.29142932838786595	No Hit
TAAGGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	230	0.27697828731078167	No Hit
TAAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	214	0.25771023254133596	No Hit
TAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	209	0.25168896542588426	No Hit
TAAGGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	207	0.2492804585797035	No Hit
TAAGGGGGGGGCACTTCCGGTGCCCTTTCTCCCATCTTTTTTAATCGTATTGGTGG	195	0.23482941750261926	No Hit
TAAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	191	0.2300124038102578	No Hit
TAAGGGGGGAACACCCTCCTGTTACAGGCTTTTAGTGGAGGAAGAATGTACAGTAT	191	0.2300124038102578	No Hit
TAAGGGGGGTCCTTTATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCC	185	0.2227868832717157	No Hit
TAAGGGGGGCAAGATACATGCACAAGGCTCTGGGTTTGAGTTCCAACTCATTAAAA	183	0.22037837642553498	No Hit
TAAGGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	177	0.21315285588699287	No Hit
TAAGGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	162	0.19508905454063752	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	159	0.19147629427136648	No Hit
TAAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	158	0.19027204084827612	No Hit
TAAGGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	148	0.17822950661737255	No Hit
TAAGGGGGGACTTCTGGGGGTCTTCCATAGCCTGCTCACAGAAAATGCAACCCCAG	140	0.1685954792326497	No Hit
TAAGGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	139	0.16739122580955937	No Hit
TAAGGGGGGATGGTGTGTGGGATCTGCATGGAGGTGGTCTATGAGAAAGCCAACCC	135	0.16257421211719794	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT	132	0.15896145184792687	No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC	131	0.15775719842483651	No Hit
TAAGGGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	129	0.1553486915786558	No Hit
TAAGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC	124	0.14932742446320402	No Hit
TAAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	121	0.14571466419393297	No Hit
TAAGGGGGGTGAGCTGTCTCTTCAGCCCCTAACTGTACTCTTATCTGTCTACTTAA	121	0.14571466419393297	No Hit
TAAGGGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT	112	0.13487638338611976	No Hit
TAAGGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	111	0.13367212996302943	No Hit
TAAGGGGGGGGAACATGGCGGACCTCAGCTTTTCTGACGGCGATCCTACTGTACGC	110	0.13246787653993908	No Hit
TAAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	109	0.13126362311684872	No Hit
TAAGGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	108	0.13005936969375834	No Hit
TAAGGGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC	98	0.1180168354628548	No Hit
TAAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCC	98	0.1180168354628548	No Hit
TAAGGGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA	97	0.11681258203976444	No Hit
TAAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAA	96	0.1156083286166741	No Hit
TAAGGGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT	96	0.1156083286166741	No Hit
TAAGGGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC	95	0.11440407519358374	No Hit
TAAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94	0.11319982177049338	No Hit
TAAGGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	94	0.11319982177049338	No Hit
TAAGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGGCT	91	0.10958706150122231	No Hit
TAAGGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG	89	0.1071785546550416	No Hit
TAAGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGC	89	0.1071785546550416	No Hit
TAAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	88	0.10597430123195126	No Hit
TAAGGGGGGTGTTTTTTTGAGCGTTCGGTGGATGCCTTGGCATCAGGGGCCGATGA	85	0.1023615409626802	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTAT	25	1.9937806E-5	50.319954	50
CCCGTCA	45	1.2551027E-10	50.319954	50
TGGTATG	20	4.032168E-4	50.31995	50
CGTGTAT	20	4.032168E-4	50.31995	49
TTGGTAT	20	4.032168E-4	50.31995	49
CCCCTAT	15	0.008194147	50.31995	50
GATTAAG	40	2.4865585E-9	50.31995	50
GCCAACC	15	0.008194147	50.31995	49
TGTACGC	30	9.913565E-7	50.31995	50
TTCAGGT	35	4.9540176E-8	50.31995	50
CATGTGT	15	0.008194147	50.31995	50
TTATCTC	15	0.008213882	50.289364	47
TACATGT	15	0.008213882	50.289364	48
ATTATCT	15	0.008213882	50.289364	46
TATCTCA	15	0.008213882	50.289364	48
CGTATTG	15	0.008213882	50.289364	46
TGTACAG	25	2.000976E-5	50.289364	47
CTACTGT	30	9.955293E-7	50.289364	46
CTCATTA	20	4.0442985E-4	50.28936	47
GTGTGTA	20	4.0442985E-4	50.28936	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content