##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212632_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66436 Sequences flagged as poor quality 0 Sequence length 58 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.21178276837859 38.0 36.0 39.0 33.0 40.0 2 36.68748871093985 38.0 36.0 39.0 31.0 40.0 3 36.62865012944789 38.0 36.0 39.0 31.0 40.0 4 35.96581672587151 38.0 35.0 39.0 30.0 40.0 5 36.176515744475886 38.0 35.0 39.0 30.0 40.0 6 36.38008007706665 38.0 35.0 40.0 30.0 40.0 7 36.03416822204829 38.0 35.0 39.0 30.0 40.0 8 35.488771148172674 38.0 35.0 40.0 27.0 40.0 9 35.10962430007827 38.0 34.0 40.0 25.0 40.0 10 35.537931242097656 38.0 35.0 39.0 28.0 40.0 11 36.17127762056716 38.0 35.0 39.0 30.0 40.0 12 36.03866879402734 38.0 35.0 39.0 30.0 40.0 13 35.659536998013124 38.0 35.0 39.0 29.0 40.0 14 36.07623878620025 38.0 35.0 39.0 30.0 40.0 15 36.06078029983744 38.0 35.0 39.0 30.0 40.0 16 35.93365043048949 38.0 35.0 39.0 30.0 40.0 17 36.00109880185442 38.0 35.0 39.0 30.0 40.0 18 35.782407128665184 38.0 35.0 39.0 29.0 40.0 19 35.30498524896141 38.0 35.0 39.0 28.0 40.0 20 35.57580227587452 38.0 35.0 39.0 29.0 40.0 21 35.50689385273045 38.0 35.0 39.0 29.0 40.0 22 35.329204045999155 38.0 35.0 39.0 29.0 40.0 23 35.02229213077247 38.0 35.0 39.0 28.0 40.0 24 34.83344873261485 38.0 34.0 39.0 27.0 40.0 25 34.83939430429285 38.0 35.0 39.0 28.0 40.0 26 34.227617556746345 38.0 34.0 39.0 25.0 40.0 27 34.35376904088145 38.0 34.0 39.0 26.0 40.0 28 33.506472394484916 38.0 33.0 39.0 23.0 40.0 29 33.737220783912335 38.0 33.0 39.0 23.0 40.0 30 32.988003492082605 38.0 33.0 39.0 18.0 40.0 31 32.072054307905354 37.0 32.0 39.0 2.0 40.0 32 30.988485158648924 36.0 29.0 39.0 2.0 40.0 33 31.53314468059486 37.0 31.0 39.0 2.0 40.0 34 31.25042145824553 37.0 30.0 39.0 2.0 40.0 35 30.456544644469865 36.0 29.0 39.0 2.0 40.0 36 29.815265819736286 37.0 28.0 39.0 2.0 40.0 37 29.172662411945332 36.0 25.0 39.0 2.0 40.0 38 28.969052923113974 36.0 24.0 39.0 2.0 40.0 39 29.00615630080077 36.0 25.0 39.0 2.0 40.0 40 28.739734481305316 36.0 24.0 39.0 2.0 40.0 41 29.055918477933652 37.0 26.0 39.0 2.0 40.0 42 29.0125534348847 37.0 26.0 39.0 2.0 40.0 43 28.58888253356614 36.0 23.0 39.0 2.0 40.0 44 28.554925040640615 36.0 23.0 39.0 2.0 40.0 45 28.575696911313145 36.0 24.0 39.0 2.0 40.0 46 28.39300981395629 36.0 22.0 39.0 2.0 40.0 47 28.161689445481365 36.0 20.0 39.0 2.0 40.0 48 28.197754229634537 36.0 20.0 39.0 2.0 40.0 49 27.944292251189115 36.0 16.0 39.0 2.0 40.0 50 27.801703895478354 36.0 15.0 39.0 2.0 40.0 51 27.422225901619605 36.0 2.0 39.0 2.0 40.0 52 27.098350292010355 35.0 2.0 39.0 2.0 40.0 53 26.900505749894634 35.0 2.0 39.0 2.0 40.0 54 26.59067373110964 35.0 2.0 39.0 2.0 40.0 55 26.141519658016737 35.0 2.0 39.0 2.0 40.0 56 25.373351797218376 35.0 2.0 39.0 2.0 40.0 57 25.27464025528328 35.0 2.0 39.0 2.0 40.0 58 24.94364501174062 35.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1 1 -1.5087689581403225 1 2 -2.9980949363310785 1 3 -2.488440738201696 1 4 -3.4443818424358668 1 5 -1.8597164034420075 1 6 -4.485490938559867 1 7 -4.34982895729275 1 8 -5.482963471883959 1 9 -6.369448054645101 1 10 -3.897033311895264 1 11 -3.2548326236366947 1 12 -3.709803180240158 1 13 -3.7092104916595794 1 14 -3.4679594732811765 1 15 -3.8142060130268334 1 16 -4.40174726497974 1 17 -3.979117578309179 1 18 -4.7090794508234985 1 19 -5.64648493336842 1 20 -4.420515276657312 1 21 -3.676938173088228 1 22 -3.336741975753249 1 23 -3.601721804495174 1 24 -4.076175974578721 1 25 -3.626360885684619 1 26 -3.0169207521538794 1 27 -3.765362082358326 1 28 -4.625265390673505 1 29 -3.723667001529858 1 30 -4.432637416543329 1 31 -5.192123401760583 1 32 -4.977171702718451 1 33 -4.86229656407226 1 34 -3.7133716210014285 1 35 -4.836274606329482 1 36 -4.166063683667517 1 37 -4.547736570799405 1 38 -4.80512270712968 1 39 -3.8521323951586517 1 40 -4.562084464188686 1 41 -3.4877017535308177 1 42 -4.22184737972038 1 43 -3.8539342077365752 1 44 -4.662740690801385 1 45 -4.800379406333018 1 46 -6.0024158197632325 1 47 -5.651206830597609 1 48 -6.327361148576042 1 49 -5.323055436557951 1 50 -5.345241648127001 1 51 -5.617845047497866 1 52 -4.888631348639297 1 53 -5.328969772025022 1 54 -5.231938523006999 1 55 -5.829688713111306 1 56 -4.92685527387048 1 57 -4.728510015755052 1 58 -5.405494088856084 2 1 -1.0859078080701963 2 2 -2.118010784858427 2 3 -1.3012037115537325 2 4 -2.7564435535158083 2 5 -1.314134355756174 2 6 -2.053513378952573 2 7 -2.453615773562035 2 8 -3.004001340046649 2 9 -2.8715518414613683 2 10 -2.2588846442935804 2 11 -2.135618037381434 2 12 -2.8213038815024305 2 13 -2.0044419082093654 2 14 -2.5177490945995515 2 15 -2.2630138671642825 2 16 -3.1920698456249 2 17 -2.6726659654059546 2 18 -2.8977190020156485 2 19 -3.164016490170667 2 20 -3.6372053187330486 2 21 -3.056321062288788 2 22 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0.12502806642832098 3 6 -0.6098235091166941 3 7 -0.8441081334941245 3 8 -1.0008887197863245 3 9 -0.17036338645288396 3 10 -0.5842949442369871 3 11 0.5514221437164721 3 12 0.3416842339474826 3 13 0.053566014824021124 3 14 0.3928338142855665 3 15 0.5606986399098588 3 16 0.5368492262483286 3 17 0.5822859304627528 3 18 0.6684949198858803 3 19 0.6353609857772682 3 20 0.304503792755348 3 21 -0.0034065178632118887 3 22 0.2288568038043408 3 23 0.2651736951234369 3 24 0.48713447545942046 3 25 0.7695201211803706 3 26 1.4708748237423848 3 27 1.532502143423521 3 28 1.6565805284721833 3 29 2.2439149969445893 3 30 2.1669049175527775 3 31 1.677823203883964 3 32 1.7322104483112994 3 33 1.9092518722359024 3 34 0.2278945879993337 3 35 1.1316887803982034 3 36 0.06582037430349885 3 37 0.11435343682835963 3 38 0.29518240347291425 3 39 -0.1110950191098361 3 40 -0.08763747715588366 3 41 0.5897200618772693 3 42 0.4192662739790869 3 43 0.9492694535906558 3 44 0.9924843282680271 3 45 0.6252498842848304 3 46 0.34998692623219085 3 47 0.5005856941926261 3 48 0.7004801939106073 3 49 0.654061268247542 3 50 0.18755774165179417 3 51 0.21968355662112415 3 52 -0.6487381373502075 3 53 -0.5295799932302074 3 54 -0.28533287846085287 3 55 -0.4269427176879681 3 56 -1.3212107277225016 3 57 -1.402804447486556 3 58 -1.02296167085456 4 1 -0.19934866828524633 4 2 0.31639781729211336 4 3 0.4912694067258414 4 4 -0.6994543062039824 4 5 0.3127473646739318 4 6 -1.1782445617482722 4 7 -0.7686695370028929 4 8 -1.1394852110143958 4 9 -0.8484335618914756 4 10 -0.1992072249387391 4 11 -0.6345427685642306 4 12 -0.32936839763146253 4 13 -0.28239889745668023 4 14 0.40160574411012817 4 15 -0.220003114476107 4 16 0.09825273502026022 4 17 0.7042157550241512 4 18 1.1141089549736023 4 19 0.4397469506895533 4 20 0.11064414363254116 4 21 0.17378646459292924 4 22 0.3183304880148654 4 23 -0.027808761016913763 4 24 0.8564327210734533 4 25 -0.5234623349599801 4 26 0.3743835956722066 4 27 0.3281161785112374 4 28 -0.07236683994887017 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0.5523108102992111 118 21 0.18107813125959638 118 22 0.9349971546815325 118 23 0.46281623898308766 118 24 -0.3404422789265453 118 25 0.10257933170668565 118 26 -0.5318664043277934 118 27 -1.1437588214887597 118 28 -0.17757517328220018 118 29 0.45636017238318516 118 30 0.3601614965001474 118 31 -0.28791144523884427 118 32 -0.03423692010975543 118 33 -0.33444330320269344 118 34 -0.6895944470883855 118 35 -0.5278506932860054 118 36 -2.569596292363169 118 37 -2.2134974403646233 118 38 -3.3649053158253324 118 39 -3.3772682647238703 118 40 -2.0865409859278152 118 41 -2.454413710052556 118 42 -2.199428901459509 118 43 -2.294151599040923 118 44 -2.1240178647144283 118 45 -2.1814935367677997 118 46 -1.551328863241494 118 47 -1.7211796566845656 118 48 -1.9231491920543036 118 49 -2.151859784384037 118 50 -1.75624708290961 118 51 -1.5961059170630847 118 52 -1.6270824355958204 118 53 -0.8187795546337178 118 54 -1.89498200126787 118 55 -1.398982191372177 118 56 -1.0484585347400461 118 57 -1.8745697983637477 118 58 -2.3110647410299983 119 1 -0.7057589246955018 119 2 -0.9271919262976311 119 3 -1.1446280291715993 119 4 -1.981505588255267 119 5 0.06402941595598577 119 6 -3.1885009720046824 119 7 -2.5096951780285366 119 8 -3.054869826399006 119 9 -3.6035617670196842 119 10 -1.5645918403233594 119 11 -1.9524914865129475 119 12 -1.310137628400696 119 13 -1.127270692328473 119 14 -1.3753173328129478 119 15 -1.4071826016555988 119 16 -1.7479011111335865 119 17 -1.7175791167707146 119 18 -1.3987115578469087 119 19 -2.5538427929001912 119 20 -1.0496122666238676 119 21 -2.1826237918173277 119 22 -2.1303874607030835 119 23 -2.0816549148630656 119 24 -2.47049035584962 119 25 -1.6337187452163917 119 26 -1.5563856350970227 119 27 -1.8526530522579918 119 28 -2.440315557897584 119 29 -2.481860981462969 119 30 -4.611232734269084 119 31 -4.373728752931154 119 32 -4.200583073955908 119 33 -4.2726644570488475 119 34 -3.956180985549924 119 35 -4.922562231747545 119 36 -3.9085386000554756 119 37 -3.7644589788261626 119 38 -3.816828392748409 119 39 -4.3073163416469455 119 40 -4.064425601312429 119 41 -4.675327171591018 119 42 -5.285726978382584 119 43 -4.649920829810153 119 44 -4.973777480099045 119 45 -4.337503152152415 119 46 -3.360944247856878 119 47 -4.001708502838412 119 48 -3.355120345900456 119 49 -3.2220520920763462 119 50 -3.015141313678839 119 51 -3.203798224755392 119 52 -3.1056882048265884 119 53 -4.244019939249103 119 54 -3.14498200126787 119 55 -2.6811937298337156 119 56 -1.8626412270477388 119 57 -2.689714029132979 119 58 -1.9911128179530735 120 1 -2.240374309310887 120 2 -2.6310380801437887 120 3 -2.4215511060946753 120 4 -4.869967126716801 120 5 -4.159047507120938 120 6 -4.7538855873892985 120 7 -5.4443105626439205 120 8 -6.439485211014393 120 9 -5.9881771516350675 120 10 -4.587668763400281 120 11 -2.590953024974482 120 12 -2.4639837822468493 120 13 -2.76188607694386 120 14 -2.098394255889872 120 15 -2.5533364478094427 120 16 -4.17097803421051 120 17 -3.3637329629245656 120 18 -3.5602500193853714 120 19 -4.103842792900192 120 20 -4.284227651239249 120 21 -3.7287776379711737 120 22 -3.7957720760876974 120 23 -4.466270299478452 120 24 -3.9474134327726986 120 25 -3.622180283677931 120 26 -3.0486933274047168 120 27 -3.3372684368733765 120 28 -3.371084788666817 120 29 -3.0857071353091214 120 30 -3.2804635034998526 120 31 -1.2506518298542275 120 32 -2.8659676893405255 120 33 -2.8380490724334635 120 34 -2.9023348317037687 120 35 -1.4725622317475455 120 36 -0.9277693692862456 120 37 -1.8336897480569299 120 38 -1.336059161979179 120 39 -1.3303932647238703 120 40 -1.6875025243893553 120 41 -0.37148101774486264 120 42 -0.5011115937672024 120 43 -0.5345362144255397 120 44 -0.18531594163750498 120 45 -1.456733921383183 120 46 -1.426328863241494 120 47 -1.0324777336076423 120 48 -1.0012741920543036 120 49 -0.9066674766917302 120 50 -1.35360285214038 120 51 -0.9422597632169314 120 52 -0.6787651279035138 120 53 -0.7286353238644878 120 54 -1.0103666166524867 120 55 -1.6658091144491003 120 56 -0.5087950732015862 120 57 -0.5704832599022076 120 58 -0.4949589717992282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 265.0 3 23.0 4 90.0 5 48.0 6 106.0 7 191.0 8 170.0 9 275.0 10 307.0 11 375.0 12 454.0 13 599.0 14 668.0 15 694.0 16 748.0 17 794.0 18 944.0 19 962.0 20 1169.0 21 1011.0 22 1431.0 23 1267.0 24 1424.0 25 1206.0 26 894.0 27 806.0 28 895.0 29 1263.0 30 1519.0 31 1857.0 32 2258.0 33 2806.0 34 3393.0 35 4000.0 36 5039.0 37 7453.0 38 9439.0 39 9579.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 47.6578963212716 40.565356132217474 5.140285387440544 6.636462159070383 11 31.534108013727497 44.84767294840147 14.224215786621711 9.394003251249321 12 66.24721536516347 14.484616774038173 11.132518514058642 8.135649346739719 13 18.131735805888376 25.63068216027455 24.06978144379553 32.167800590041544 14 24.13601059666446 47.68499006562707 17.901438978866878 10.277560358841592 15 40.16346559094467 16.1237883075441 28.174483713649227 15.538262387862003 16 15.082184357878258 20.085495815521707 48.45264615569872 16.37967367090132 17 41.69187928049974 14.46677203281403 13.459772710167833 30.3815759765184 18 37.35173701005479 17.96465771569631 21.328797639833823 23.354807634415074 19 20.490396772834004 15.902522728641097 50.89860918778976 12.708471310735145 20 20.287499059230825 35.8214796417551 21.285466997817416 22.60555430119666 21 19.651378080171 22.696551412701517 40.61686210166634 17.03520840546114 22 24.56499488229273 31.26768619423204 11.456138238304533 32.711180685170696 23 27.73688567773011 49.15330774441183 13.291186874388499 9.818619703469556 24 18.16936600638208 50.91516647600698 17.3881630441327 13.527304473478235 25 19.703172978505627 27.41585887169607 23.17869829610452 29.70226985369378 26 38.93714715844938 22.37410613473843 22.789612344749717 15.899134362062476 27 22.313713683576697 16.469968387776607 23.203371970495258 38.01294595815144 28 25.917282705250006 41.43242144567066 18.247790541863022 14.402505307216309 29 42.19131063137928 19.73022611627977 27.412458976906635 10.666004275434318 30 22.65622647191688 39.84490287607288 27.1962053907544 10.302665261255834 31 38.36076435422909 26.142540920658348 25.943772681413662 9.552922043698896 32 23.96194032097799 27.459275541236337 39.06988648339406 9.508897654391617 33 33.46129527594617 22.104296510402552 24.579231023996627 19.85517718965465 34 23.15463017328335 23.588214925552894 30.244041973412823 23.013112927750928 35 20.03974826101358 40.44084434942335 28.52690535698154 10.992502032581529 36 28.023310093511423 30.872321522685176 23.80701410953335 17.297354274270056 37 17.754954520812 28.252816095415938 39.49159689175351 14.500632492018553 38 21.687726855128997 27.877765561697064 36.295314547344006 14.139193035829933 39 19.760839771683308 29.816714107140168 30.48389282971129 19.938553291465233 40 16.829580540703365 32.90157391369832 38.835755704495824 11.433089841102491 41 17.201807228915662 33.695783132530124 37.62198795180723 11.480421686746988 42 22.358629831591 34.04181604555177 32.67857680836321 10.920977314494019 43 15.428993191540641 36.40115683557269 33.388564198349094 14.781285774537567 44 23.22775945420865 32.4312113134234 35.86349192005904 8.47753731230892 45 21.841019307810477 32.09132805204976 35.54022711527456 10.527425524865206 46 16.74874621605747 33.608939893673096 35.56981279838552 14.072501091883913 47 21.24248496993988 34.767278918739734 32.819021501649914 11.171214609670468 48 23.59357579775213 32.48711844999548 31.928164642781816 11.991141109470576 49 23.34353008544175 34.277663085245855 31.54262292611624 10.836183903196154 50 16.56242935724512 35.603948459046045 33.74274734383242 14.090874839876422 51 19.605863830171906 36.032724149880224 29.51350699832763 14.847905021620237 52 20.093999969871803 35.468418119096754 33.70139945467967 10.736182456351777 53 12.306162123061622 34.391247343912475 37.893515378935156 15.40907515409075 54 15.969375452134074 37.192548830479865 30.58385579937304 16.25421991801302 55 23.048859542007747 32.109206578927534 32.1664932989613 12.675440580103418 56 17.941952506596305 37.84093479080287 32.735770825480586 11.48134187712024 57 21.45150834451463 34.93841491911776 29.268366223937527 14.341710512430087 58 20.75656605252842 31.46880559659862 31.085848686789497 16.688779664083466 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 88.5 7 177.0 8 182.0 9 152.5 10 118.0 11 116.5 12 115.0 13 177.0 14 239.0 15 300.0 16 368.0 17 375.0 18 521.0 19 667.0 20 744.0 21 821.0 22 1024.0 23 1727.0 24 2227.0 25 2829.5 26 3432.0 27 3782.5 28 3462.5 29 2792.0 30 3994.0 31 5196.0 32 3574.0 33 1952.0 34 1668.5 35 1301.5 36 1218.0 37 1165.0 38 1112.0 39 1169.5 40 1227.0 41 1832.0 42 3284.5 43 4132.0 44 3242.5 45 2353.0 46 2570.5 47 2845.0 48 2902.0 49 4502.0 50 6102.0 51 5074.5 52 4047.0 53 3531.5 54 2635.5 55 2255.0 56 2000.0 57 1745.0 58 1776.0 59 1631.5 60 1456.0 61 1331.5 62 1207.0 63 968.5 64 730.0 65 506.0 66 205.5 67 129.0 68 85.0 69 41.0 70 30.5 71 20.0 72 17.0 73 9.0 74 4.0 75 3.0 76 2.0 77 2.0 78 2.0 79 2.0 80 1.5 81 1.0 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0015052080197483291 18 0.0 19 0.0 20 0.0015052080197483291 21 0.004515624059244988 22 0.0 23 0.0015052080197483291 24 0.0 25 0.0 26 0.01655728821723162 27 0.009031248118489975 28 0.0255885363357216 29 0.01505208019748329 30 0.03913540851345656 31 0.04064061653320489 32 0.021072912276476608 33 0.01505208019748329 34 0.01956770425672828 35 0.027093744355469927 36 0.04064061653320489 37 0.04816665663194653 38 0.058703112770184834 39 0.05569269673068818 40 0.06171352880968149 41 0.054187488710939855 42 0.07526040098741646 43 0.0722499849479198 44 0.05569269673068818 45 0.05719790475043651 46 0.05569269673068818 47 0.10385935336263472 48 0.0933228972243964 49 0.11289060148112469 50 0.12192184959961468 51 0.09482810524414474 52 0.07977602504666145 53 0.118911433560118 54 0.12643747365885966 55 0.1550364260340779 56 0.16707809019206454 57 0.15804684207357458 58 0.16557288217231622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 66436.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.10054789571919 #Duplication Level Percentage of deduplicated Percentage of total 1 77.03834729499717 34.74471671985068 2 11.263892133631478 10.160154133301221 3 4.7792277141808235 6.466373652838822 4 2.29950272002136 4.148353302426395 5 1.2682308180088777 2.8598952375218256 6 0.7709508393685546 2.086218315371184 7 0.4972799786403231 1.5699319645975076 8 0.38714414444481526 1.3968330423264497 9 0.22694656743316755 0.9211873080859775 >10 1.2782431665721057 10.108977060629778 >50 0.08009878850582385 2.361671382985129 >100 0.08343623802689984 7.700644229032452 >500 0.013349798084303973 3.818712746101511 >1k 0.013349798084303973 11.656330904931062 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 2933 4.41477512192185 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 2270 3.4168222048287076 No Hit AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1444 2.1735203805165875 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1097 1.651213197663917 No Hit AAAGTTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 860 1.294478896983563 No Hit AAAGTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 632 0.951291468480944 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGTGG 524 0.7887290023481245 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC 521 0.7842133782888795 No Hit AAAGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 488 0.7345415136371847 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 479 0.7209946414594497 No Hit AAAGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 474 0.713468601360708 No Hit AAAGTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 363 0.5463905111686435 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC 292 0.4395207417665121 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 273 0.41092178939129387 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGAAC 217 0.32663014028538745 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 204 0.3070624360286591 Illumina Single End Adapter 2 (96% over 25bp) AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 195 0.2935155638509242 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 186 0.2799686916731892 No Hit AAAGTTGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 182 0.2739478595941959 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 157 0.2363176591004877 No Hit AAAGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 155 0.23330724306099104 No Hit AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.23330724306099104 No Hit AAAGTTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT 148 0.22277078692275273 No Hit AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.18965621048828948 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 125 0.18815100246854116 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGGGG 123 0.1851405864290445 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGAAGCTGAAAAACATCGAAC 117 0.1761093383105545 No Hit AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 116 0.1746041302908062 No Hit AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAG 114 0.17159371425130954 No Hit AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.1640676741525679 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.16105725811307123 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA 107 0.16105725811307123 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 104 0.15654163405382623 Illumina Single End Adapter 2 (96% over 32bp) AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 87 0.13095309771810465 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.12643747365885966 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.12643747365885966 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAA 76 0.11439580950087302 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG 75 0.11289060148112469 Illumina Single End Adapter 2 (96% over 30bp) AAAGTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC 75 0.11289060148112469 Illumina Single End Adapter 2 (100% over 31bp) AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC 74 0.11138539346137637 No Hit AAAGTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG 67 0.10084893732313804 No Hit AAAGTTGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 67 0.10084893732313804 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.05719790475043651 0.0 0.0 0.0 0.0 11 0.09181768920464808 0.0 0.0 0.0 0.0 12 0.09482810524414474 0.0 0.0 0.0 0.0 13 0.0978385212836414 0.0 0.0 0.0 0.0 14 0.12493226563911132 0.0 0.0 0.0 0.0 15 0.13095309771810465 0.0 0.0 0.0 0.0 16 0.13245830573785297 0.0 0.0 0.0 0.0 17 0.13546872177734964 0.0 0.0 0.0 0.0 18 0.13697392979709797 0.0 0.0 0.0 0.0 19 0.1399843458365946 0.0 0.0 0.0 0.0 20 0.1806249623697995 0.0 0.0 0.0 0.0 21 0.18815100246854116 0.0 0.0 0.0 0.0 22 0.22126557890300438 0.0 0.0 0.0 0.0 23 0.24384369919922932 0.0 0.0 0.0 0.0 24 0.26943223553495094 0.0 0.0 0.0 0.0 25 0.2754530676139442 0.0 0.0 0.0 0.0 26 0.28147389969293757 0.0 0.0 0.0 0.0 27 0.29201035583117585 0.0 0.0 0.0 0.0 28 0.45607802998374375 0.0 0.0 0.0 0.0 29 0.4681196941417304 0.0 0.0 0.0 0.0 30 0.5283280149316636 0.0 0.0 0.0 0.0 31 0.5403696790896502 0.0 0.0 0.0 0.0 32 0.5494009272081402 0.0 0.0 0.0 0.0 33 0.5539165512673851 0.0 0.0 0.0 0.0 34 0.5614425913661268 0.0 0.0 0.0 0.0 35 0.8052862905653562 0.0 0.0 0.0 0.0 36 0.8188331627430911 0.0 0.0 0.0 0.0 37 0.8263592028418327 0.0 0.0 0.0 0.0 38 0.8474321151183094 0.0 0.0 0.0 0.0 39 0.8609789872960443 0.0 0.0 0.0 0.0 40 0.8790414835330242 0.0 0.0 0.0 0.0 41 0.8940935637305075 0.0 0.0 0.0 0.0 42 0.9061352278884942 0.0 0.0 0.0 0.0 43 0.9272081401649708 0.0 0.0 0.0 0.0 44 0.9527966765006924 0.0 0.0 0.0 0.0 45 0.9768800048166657 0.0 0.0 0.0 0.0 46 1.0039737491721357 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTGG 15 0.0071681 52.040726 42 GCGGGGC 15 0.0071681 52.040726 44 GTTTGGT 15 0.0071681 52.040726 44 TACAGGC 15 0.0071681 52.040726 49 GCGATTA 15 0.0071681 52.040726 44 TGGTAGG 15 0.0071681 52.040726 47 ACGTTGG 15 0.0071681 52.040726 42 CGGGGCC 15 0.0071681 52.040726 45 GGCCGCC 30 7.8380435E-7 52.040726 50 GCAGGCC 100 0.0 52.040726 47 CCGCCCC 30 7.8380435E-7 52.040726 52 TCGAACT 65 0.0 52.040726 52 GAAAAGC 25 1.6306227E-5 52.040726 45 GGCGATT 15 0.0071681 52.040726 43 ATTACAG 15 0.0071681 52.040726 47 CCTTCTC 100 0.0 52.040726 50 AGGCCGC 100 0.0 52.040726 49 AGCATCG 25 1.6306227E-5 52.040726 49 TTTGGCG 15 0.0071681 52.040726 45 TGGGTAG 25 1.6306227E-5 52.040726 46 >>END_MODULE