FastQCFastQC Report
Tue 31 May 2016
SRR212631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65849
Sequences flagged as poor quality0
Sequence length58
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT28264.2916369269085335No Hit
AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC21903.3257908244620262No Hit
AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG14902.2627526613919726No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10541.6006317483940529No Hit
AAAGTTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT8691.3196859481541101No Hit
AAAGTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC6380.9688833543409923No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4900.744126714149038No Hit
AAAGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4820.7319777065710945No Hit
AAAGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4820.7319777065710945No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC4180.6347856459475466No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGTGG3970.6028945010554451No Hit
AAAGTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA3260.4950720588011967No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2760.41914076143905No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.3644702273383043No Hit
AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGAAC2320.35232121976036085No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1990.302206563501344Illumina Single End Adapter 2 (96% over 25bp)
AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1860.2824644261871858No Hit
AAAGTTGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1770.2687967926619994No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1760.2672781667147565No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.2369056477698978No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.23538702182265483No Hit
AAAGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1520.23083114398092605No Hit
AAAGTTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT1460.22171938829746843No Hit
AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGAAGCTGAAAAACATCGAAC1370.20805175477228202No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.1959027471943386No Hit
AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.17464198393293748No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1120.17008610609120867Illumina Single End Adapter 2 (96% over 32bp)
AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.16401160230223694No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.14275083904083585No Hit
AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAG940.14275083904083585No Hit
AAAGTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG870.1321204574101353No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAA860.13060183146289236No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.13060183146289236No Hit
AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.12908320551564945No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA830.12604595362116355No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.12452732767392063No Hit
AAAGTTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG820.12452732767392063No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA790.11997144983219184No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGGGG760.11541557199046303No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA760.11541557199046303No Hit
AAAGTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGA760.11541557199046303No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC730.11085969414873421No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG700.10630381630700543No Hit
AAAGTTGGGCAGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA700.10630381630700543No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT690.1047851903597625No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC690.1047851903597625No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG670.10174793846527662Illumina Single End Adapter 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG458.913048E-1152.10053622
AACGCGG251.6194E-552.10053323
AACGCCG150.00713530952.10053323
TGCCGAA150.00713530952.10053322
TGCGAAG150.00713530952.10053325
CAACACA203.391261E-452.10053322
TCAACAC251.6194E-552.10053321
CAGAGAG307.774979E-752.10053327
CAGGGTC251.6194E-552.10053327
GATCCCA150.00713530952.10053323
CGCAGGA251.6194E-552.10053325
CTGCCGA150.00713530952.10053321
ACGCGGA150.00713530952.10053324
ACGCGAT150.00713530952.10053324
CAACGCC150.00713530952.10053322
AGAAGTG251.634081E-552.02129451
GGCCGCT1050.052.02129450
CCGCTCC1050.052.02129452
TTCTCCT1050.052.02129452
GCCGCTC1050.052.02129451
Produced by FastQC (version 0.11.5)