##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212631_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65849 Sequences flagged as poor quality 0 Sequence length 58 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.17617579613965 38.0 36.0 39.0 33.0 40.0 2 36.72482497835958 38.0 36.0 39.0 31.0 40.0 3 36.64664611459551 38.0 36.0 39.0 31.0 40.0 4 36.071694330969336 38.0 35.0 39.0 30.0 40.0 5 36.10615195371228 38.0 35.0 39.0 30.0 40.0 6 36.477987516894714 38.0 36.0 39.0 31.0 40.0 7 36.12577260095066 38.0 35.0 39.0 30.0 40.0 8 35.66005558170967 38.0 35.0 39.0 28.0 40.0 9 35.33218423969992 38.0 35.0 39.0 25.0 40.0 10 35.52319701134414 38.0 35.0 39.0 28.0 40.0 11 36.14958465580343 38.0 35.0 39.0 30.0 40.0 12 35.992665036674815 38.0 35.0 39.0 30.0 40.0 13 35.560646327203145 38.0 35.0 39.0 29.0 40.0 14 36.03656851280961 38.0 35.0 39.0 30.0 40.0 15 36.04574101353096 38.0 35.0 39.0 30.0 40.0 16 35.88335434099227 38.0 35.0 39.0 30.0 40.0 17 35.95981715743595 38.0 35.0 39.0 30.0 40.0 18 35.74195507904448 38.0 35.0 39.0 29.0 40.0 19 35.32310285653541 38.0 35.0 39.0 29.0 40.0 20 35.55095749365974 38.0 35.0 39.0 29.0 40.0 21 35.53920332882807 38.0 35.0 39.0 29.0 40.0 22 35.35667967622895 38.0 35.0 39.0 29.0 40.0 23 35.04161035095446 38.0 35.0 39.0 28.0 40.0 24 34.83601877021671 38.0 34.0 39.0 27.0 40.0 25 34.88207869519658 38.0 35.0 39.0 28.0 40.0 26 34.16704885419672 38.0 33.0 39.0 26.0 40.0 27 33.94944494221628 38.0 33.0 39.0 24.0 40.0 28 33.47991617184771 38.0 33.0 39.0 23.0 40.0 29 33.67931175872071 38.0 33.0 39.0 23.0 40.0 30 32.933666418624426 38.0 33.0 39.0 18.0 40.0 31 32.057783717292594 37.0 32.0 39.0 2.0 40.0 32 30.973029203176967 36.0 29.0 39.0 2.0 40.0 33 31.523105893787303 37.0 31.0 39.0 2.0 40.0 34 31.250998496560314 37.0 30.0 39.0 2.0 40.0 35 30.37131923036037 36.0 29.0 39.0 2.0 40.0 36 29.863429968564443 37.0 28.0 39.0 2.0 40.0 37 29.257437470576622 36.0 26.0 39.0 2.0 40.0 38 29.09357773086911 36.0 25.0 39.0 2.0 40.0 39 29.109933332320917 36.0 25.0 39.0 2.0 40.0 40 28.85229844037115 36.0 25.0 39.0 2.0 40.0 41 29.15866603896794 36.0 27.0 39.0 2.0 40.0 42 29.114231043751612 36.0 26.0 39.0 2.0 40.0 43 28.69382982277635 36.0 24.0 39.0 2.0 40.0 44 28.642712873392156 36.0 24.0 39.0 2.0 40.0 45 28.68187823657155 36.0 25.0 39.0 2.0 40.0 46 28.445868578110524 36.0 23.0 39.0 2.0 40.0 47 28.16759555953773 36.0 20.0 39.0 2.0 40.0 48 28.237209372959345 36.0 21.0 39.0 2.0 40.0 49 27.97842032528968 36.0 18.0 39.0 2.0 40.0 50 27.805615878752903 35.0 17.0 39.0 2.0 40.0 51 27.46614223450622 36.0 2.0 39.0 2.0 40.0 52 27.10088232167535 35.0 2.0 39.0 2.0 40.0 53 26.871190147154856 35.0 2.0 39.0 2.0 40.0 54 26.578444623304833 35.0 2.0 39.0 2.0 40.0 55 26.162492976354994 35.0 2.0 39.0 2.0 40.0 56 25.305456423028446 35.0 2.0 39.0 2.0 40.0 57 25.168795274036054 35.0 2.0 39.0 2.0 40.0 58 24.8763990341539 35.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.38803442824716683 1 2 -1.1854198995215341 1 3 -1.2363365018287027 1 4 -1.8516411949843103 1 5 -0.9519553270384691 1 6 -1.968566819712187 1 7 -2.546221928993532 1 8 -2.495299845219755 1 9 -2.551839249943022 1 10 -1.7984632448924813 1 11 -1.4656131043449392 1 12 -2.216184494144521 1 13 -2.1482420232069117 1 14 -3.049090623787599 1 15 -2.337750822601322 1 16 -0.49872461809767543 1 17 -1.1133882883203512 1 18 -1.611588629031118 1 19 -1.3797372785375899 1 20 -0.6104605642178811 1 21 -1.0368790170034003 1 22 -1.0816625291486233 1 23 -0.7781446904526987 1 24 -0.5462226965417472 1 25 -0.41546942847159585 1 26 -1.7189201806614562 1 27 -1.4320450775220905 1 28 -2.9978679020479966 1 29 -2.3145817671048015 1 30 -1.8918004727773194 1 31 -1.5263352871923814 1 32 -1.4354329069474758 1 33 -1.165880301235756 1 34 -1.1200177716708666 1 35 -1.5105889991534944 1 36 -0.5916573481177956 1 37 -0.610619286319956 1 38 -0.3489627800675734 1 39 -1.4589066112398932 1 40 -1.3265136883943178 1 41 -0.984155489036926 1 42 -1.6632043483771284 1 43 -1.5642614110907687 1 44 -1.642366580591986 1 45 -0.6401513202438238 1 46 -0.8724940949605369 1 47 -1.0965337620174544 1 48 -0.8961130788833955 1 49 -0.45752776260332695 1 50 -0.4701955598896177 1 51 -0.11444389060191895 1 52 -0.31159944594805467 1 53 -0.5384544366962274 1 54 -0.6290391583082666 1 55 -0.05248201968964494 1 56 -1.034619695786347 1 57 -0.3562139800579729 1 58 -0.30128926061349404 2 1 -1.4843769795406558 2 2 -1.2564873987781553 2 3 -1.3359499422093108 2 4 -3.5233628720583567 2 5 -1.2968854489534252 2 6 -2.748525190214714 2 7 -3.938728619448483 2 8 -4.2131113230669115 2 9 -4.763852333499372 2 10 -3.0431852193200726 2 11 -2.5244891079131833 2 12 -2.48201857086174 2 13 -1.7411650086365853 2 14 -2.4044281200706763 2 15 -2.547979784837416 2 16 -1.8484123968666353 2 17 -2.3441346457393237 2 18 -1.6145621645648731 2 19 -3.6461660623615586 2 20 -2.234010965645176 2 21 -2.358020854648359 2 22 -3.917523367685643 2 23 -2.8355339262540653 2 24 -3.7070909689176013 2 25 -3.5350055569283327 2 26 -3.3094643376641315 2 27 -2.8367432636654115 2 28 -3.8934968048133847 2 29 -5.207831841443188 2 30 -3.282225688358647 2 31 -3.371116732330652 2 32 -2.7880942212501836 2 33 -3.141497309859009 2 34 -1.8978946672997665 2 35 -2.6845408278778464 2 36 -1.8307296050312658 2 37 -1.1830248765667584 2 38 -1.0950228454853566 2 39 -0.43868656961039676 2 40 -1.146614788602463 2 41 -1.725160544047334 2 42 -1.9198204649397184 2 43 -1.2496613516200554 2 44 -1.1395417218349237 2 45 -1.5458902438239583 2 46 -0.9498060188380286 2 47 -1.393292608285666 2 48 -0.7973916991629082 2 49 -0.6680540783927995 2 50 -1.082149172735292 2 51 -1.6627638430253526 2 52 -0.9069012598047337 2 53 -1.0347375172790407 2 54 -1.4711444214661604 2 55 -0.8654466346167915 2 56 -3.278449609553814 2 57 -4.245598458202487 2 58 -2.926921136914416 3 1 -1.3082646914050642 3 2 -1.7350361275917123 3 3 -1.8311829930567711 3 4 -2.0736806686685227 3 5 -1.518018923529695 3 6 -1.9617137495367487 3 7 -2.593919297414587 3 8 -2.5144884417109807 3 9 -3.009350214855303 3 10 -2.0021364905065155 3 11 -0.9055802096080967 3 12 -2.0114147573024184 3 13 -0.8269700933823501 3 14 -1.2502967641384757 3 15 -1.6020052085662329 3 16 -0.9082640917818878 3 17 -0.6079058321800019 3 18 -1.6368079272767346 3 19 -2.0675114013446105 3 20 -0.8020724063231413 3 21 -2.1199382275297154 3 22 -3.145807265990726 3 23 -2.611752146593048 3 24 -2.2293367316294663 3 25 -2.082410217945281 3 26 -2.4368478122404014 3 27 -2.292516568750159 3 28 -2.1220455336269453 3 29 -2.712333960087257 3 30 -1.7999693324264427 3 31 -1.30676291877133 3 32 -0.5889416788773012 3 33 -1.1497070556217217 3 34 -2.0048861927234967 3 35 -2.0640429465219157 3 36 -1.5192889270651655 3 37 -1.793459198600658 3 38 -2.404609709892135 3 39 -1.5811653831697186 3 40 -2.456996144534667 3 41 -2.687554611843943 3 42 -2.842754786973618 3 43 -2.392934656704803 3 44 -2.527783247258654 3 45 -2.625074565857858 3 46 -3.2938208493465027 3 47 -2.4290447269297353 3 48 -2.7807073771290085 3 49 -2.9180540783927995 3 50 -3.0697021388369876 3 51 -3.1023824870931485 3 52 -3.0917529547199862 3 53 -2.9913053138892103 3 54 -2.9086444214661604 3 55 -2.7068131600405216 3 56 -2.540102151926696 3 57 -2.5149310853211304 3 58 -2.3043046114906858 4 1 -1.0989921504214593 4 2 -0.9622389964441709 4 3 -0.7405067635485736 4 4 -1.5444798489963887 4 5 -0.504443103857561 4 6 -2.067502683962978 4 7 -2.3054971662670454 4 8 -2.1789351630224587 4 9 -2.4537661984618637 4 10 -1.7472696872278277 4 11 -1.6942584882966187 4 12 -1.6156668064827429 4 13 -2.147666814693828 4 14 -1.982366436269622 4 15 -1.8099970118449207 4 16 -1.2243501573556586 4 17 -1.658110750212792 4 18 -1.2566849764570591 4 19 -1.550861811180674 4 20 -1.6363449473067462 4 21 -0.49416978490676655 4 22 -0.2723441512366307 4 23 -0.3645697695438699 4 24 -0.31642689556389314 4 25 -0.17129341466659298 4 26 0.1715025975956621 4 27 0.4408338410859045 4 28 0.38435815489764735 4 29 0.6951967776176602 4 30 0.4349179626555255 4 31 0.6427760156548992 4 32 -0.8553351215002536 4 33 -0.725270580211884 4 34 0.7686793810469936 4 35 0.10373369282234535 4 36 -1.021338107393035 4 37 -1.750682559256397 4 38 -2.415111759072463 4 39 -1.5509399733336515 4 40 -1.0932666363379475 4 41 -1.3683947757783699 4 42 -1.5530519181211595 4 43 -1.9065104763769334 4 44 -1.3582135751275075 4 45 -0.4742036642185141 4 46 -1.1859827345924039 4 47 -0.7853459564379328 4 48 -1.2625209017191708 4 49 -1.9795294882288665 4 50 -2.0474173027714144 4 51 -1.8800464215193777 4 52 -2.643494757998674 4 53 -1.8263462974957676 4 54 -2.9301608149087848 4 55 -3.821823405942162 4 56 -3.1784218240578426 4 57 -3.7651872328621145 4 58 -4.150872234441504 5 1 -0.9186642815690007 5 2 -1.0605996521818781 5 3 -1.5765723373190639 5 4 -1.4133323080127838 5 5 -0.6847709727100195 5 6 -1.6084862905203536 5 7 -0.6989397892178673 5 8 -1.883853195809344 5 9 -1.9783563623962834 5 10 -1.2390729659163497 5 11 -0.7270453735425235 5 12 -0.6812405769745524 5 13 -1.0656996015790696 5 14 -1.5725303706958513 5 15 -0.5804888151236085 5 16 -0.6833665507982829 5 17 -0.5105697666062312 5 18 0.13675764649375566 5 19 -0.1902060734757569 5 20 -0.19372199648707777 5 21 0.42386300197848215 5 22 0.7768361766322229 5 23 0.40592203373481794 5 24 -0.16888591195733227 5 25 -0.17129341466659298 5 26 0.7780599746448473 5 27 0.7687026935449239 5 28 0.8105876630943669 5 29 1.6624098923717625 5 30 1.2218032085571622 5 31 1.282120277949982 5 32 -0.03566299035271214 5 33 0.8484999115913929 5 34 1.2604826597355228 5 35 0.021766479707594044 5 36 1.3229241876889333 5 37 1.0689895718911444 5 38 1.388166929452126 5 39 1.432666584043396 5 40 -0.07687319371499512 5 41 1.156195388156057 5 42 1.3158005408952356 5 43 1.044309195754213 5 44 0.7073601953642985 5 45 1.7880914177486993 5 46 0.5517221834403827 5 47 0.017932732086659797 5 48 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0.25261208122867274 6 32 0.0072121672765455 6 33 0.3380333289936637 6 34 -0.7596938850311901 6 35 -1.065244869598839 6 36 0.8941726113963746 6 37 0.634185032168574 6 38 0.4499575978001715 6 39 0.9529692322148975 6 40 0.6189653939268709 6 41 1.294656926617595 6 42 1.1127740591802286 6 43 1.2087480356028877 6 44 1.0294282912028834 6 45 0.7943965879882953 6 46 1.292957996807342 6 47 0.9490802730702654 6 48 1.2315522382556061 6 49 1.6242536139148918 6 50 1.1079420919322445 6 51 0.2024252052145421 6 52 0.7423816606646305 6 53 0.8288869938030992 6 54 0.19039404007230232 6 55 0.10352337842101633 6 56 -0.4276021519266955 6 57 -0.8469022391672851 6 58 -1.142044996106069 7 1 -0.41658036937116805 7 2 -0.42597892420187833 7 3 -0.40374655237880575 7 4 -0.47251541443123557 7 5 0.025148449351661384 7 6 -0.985813325807932 7 7 -1.7522879414823862 7 8 -2.2131113230669115 7 9 -3.0350387741773375 7 10 -0.4160665752522803 7 11 -0.4058450401165743 7 12 -1.2108321301837748 7 13 -0.8259107713484539 7 14 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7 55 -4.153582227837131 7 56 -4.193703846841949 7 57 -5.432039136168587 7 58 -4.672683848778821 8 1 -0.7801396914050613 8 2 -1.9485777942583766 8 3 -1.2249329930567683 8 4 -2.1840973353351885 8 5 -0.24406059019636217 8 6 -2.4273387495367515 8 7 -2.058502630747917 8 8 -1.3874051083776493 8 9 -2.306225214855303 8 10 -1.484428157173184 8 11 -0.5357885429414324 8 12 -0.9916230906357484 8 13 -1.2394700933823515 8 14 -0.9815467641384785 8 15 -0.8728385418995686 8 16 -0.5478474251152221 8 17 -0.6704058321800019 8 18 -1.7826412606100703 8 19 -0.6393864013446091 8 20 -0.3051974063231384 8 21 -0.3272298941963854 8 22 -0.3228905993240616 8 23 -0.471127146593048 8 24 -0.5918367316294635 8 25 -0.8584518846119451 8 26 -0.28997281224039995 8 27 -0.5873082354168275 8 28 0.047746133039723304 8 29 0.2512077065794074 8 30 -0.3093443324264413 8 31 1.0859454145620013 8 32 1.894391654456033 8 33 0.6534179443782797 8 34 0.3826138072765026 8 35 1.1078320534780843 8 36 0.5557110729348338 8 37 1.0200824680660077 8 38 -0.024401376558802923 8 39 0.4542512834969479 8 40 1.3305038554653343 8 41 -0.1771379451772752 8 42 1.2832868796930477 8 43 1.133107009961865 8 44 0.824300086074679 8 45 1.8072171008088098 8 46 1.8134708173201624 8 47 2.0824136064035983 8 48 1.7995009562043265 8 49 1.5819459216072005 8 50 2.077172861163014 8 51 1.6601175129068508 8 52 2.226997045280015 8 53 2.171194686110791 8 54 2.178855578533838 8 55 2.781728506626145 8 56 3.355731181406636 8 57 3.390277248012204 8 58 3.925903721842648 9 1 -0.14085397711934888 9 2 -0.13905398473456643 9 3 -0.049932993056771124 9 4 -0.09600209723995334 9 5 -0.12739392352969503 9 6 -0.5130530352510334 9 7 -0.21445501170030212 9 8 -0.8314527274252654 9 9 -0.21693950056958755 9 10 -1.1427614905065155 9 11 0.6725447903919033 9 12 -0.6654326144452725 9 13 -0.532327236239496 9 14 -1.102975335567045 9 15 -1.5573623514233788 9 16 -1.0109426632104572 9 17 -1.652548689322856 9 18 -1.1100222129910193 9 19 -0.9893864013446105 9 20 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-0.13767053362694526 118 29 -0.8217089600872569 118 30 -0.7062193324264427 118 31 0.036987081228669894 118 32 -0.9014416788773012 118 33 -0.6028320556217217 118 34 -1.1142611927234967 118 35 -1.9702929465219157 118 36 -1.2067889270651655 118 37 -1.340334198600658 118 38 -0.6233597098921351 118 39 -0.9405403831697186 118 40 0.16800385546533292 118 41 -0.8906796118439431 118 42 -1.280254786973618 118 43 -1.1898096567048029 118 44 -0.5590332472586539 118 45 -0.46882456585785803 118 46 -0.6063208493465027 118 47 -0.8821697269297353 118 48 -0.5150823771290085 118 49 -0.9961790783927995 118 50 -0.4603271388369876 118 51 -0.7430074870931485 118 52 -0.6073779547199862 118 53 -0.3350553138892103 118 54 -0.22114442146616042 118 55 0.09006183995947836 118 56 0.1473978480733038 118 57 0.2975689146788696 118 58 0.7738203885093142 119 1 -0.13638969140506418 119 2 -0.5944111275917123 119 3 -0.5186829930567711 119 4 -0.9174306686685227 119 5 -0.03364392352969503 119 6 -1.3367137495367487 119 7 -1.9689192974145868 119 8 -1.5144884417109807 119 9 -2.040600214855303 119 10 0.49786350949348446 119 11 0.5944197903919033 119 12 0.5510852426975816 119 13 0.8761549066176499 119 14 0.7653282358615243 119 15 0.05424479143376715 119 16 0.6854859082181122 119 17 -0.18603083218000194 119 18 -0.15243292727673463 119 19 0.11998859865538947 119 20 0.36980259367685875 119 21 -0.04181322752971539 119 22 -0.03643226599072591 119 23 -0.252377146593048 119 24 -0.5262117316294663 119 25 -1.4574102179452808 119 26 -1.5774728122404014 119 27 -1.651891568750159 119 28 -0.48142053362694526 119 29 -0.4154589600872569 119 30 -1.4718443324264427 119 31 -0.1973879187713301 119 32 -0.5264416788773012 119 33 -0.3840820556217217 119 34 -0.8642611927234967 119 35 -1.2515429465219157 119 36 -1.4880389270651655 119 37 -1.105959198600658 119 38 -1.638984709892135 119 39 -0.8467903831697186 119 40 -1.503871144534667 119 41 -0.7500546118439431 119 42 -1.030254786973618 119 43 -1.0491846567048029 119 44 -0.6527832472586539 119 45 -0.671949565857858 119 46 -0.15319584934650265 119 47 -0.2571697269297353 119 48 -0.015082377129008506 119 49 -1.0274290783927995 119 50 -0.8040771388369876 119 51 0.17886751290685154 119 52 -0.21675295471998623 119 53 -0.4131803138892103 119 54 -0.7680194214661604 119 55 -1.4255631600405216 119 56 -1.7901021519266962 119 57 -1.7024310853211304 119 58 -1.6793046114906858 120 1 -1.4801396914050642 120 2 -2.4069111275917123 120 3 -2.299932993056771 120 4 -3.631716382954238 120 5 -1.4488224949582644 120 6 -3.0309101781081793 120 7 -2.500169297414587 120 8 -4.045738441710981 120 9 -4.431225214855303 120 10 -2.964190061935085 120 11 -2.4524552096080967 120 12 -2.1297183287309878 120 13 -3.9787558076680654 120 14 -2.4065467641384757 120 15 -2.8073623514233788 120 16 -3.0109426632104572 120 17 -3.4918344036085713 120 18 -2.8778793558481652 120 19 -3.453672115630326 120 20 -3.165911692037426 120 21 -3.671277513244 120 22 -2.507414408847872 120 23 -2.578270003735902 120 24 -2.9168367316294663 120 25 -3.6382137893738538 120 26 -2.675687097954686 120 27 -2.1384987116073013 120 28 -3.9189205336269453 120 29 -1.7368875315158299 120 30 -2.748630046712158 120 31 -2.893816490199903 120 32 -4.571084536020159 120 33 -2.1430106270502947 120 34 -3.1923861927234967 120 35 -4.374310803664773 120 36 -4.912146069922308 120 37 -5.121584198600658 120 38 -4.107734709892135 120 39 -5.094558240312576 120 40 -5.483781858820382 120 41 -4.486661754701085 120 42 -5.429808358402191 120 43 -5.479988228133376 120 44 -5.023318961544369 120 45 -4.823735280143573 120 46 -4.296052992203645 120 47 -3.88663401264402 120 48 -3.715975234271866 120 49 -3.328768364107084 120 50 -4.004969995979845 120 51 -3.1113110585217214 120 52 -2.9622886690057015 120 53 -2.214519599603495 120 54 -2.5425729928947334 120 55 -2.5349381600405216 120 56 -2.4418878662124115 120 57 -2.6823417996068457 120 58 -2.4895724686335434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 255.0 3 23.0 4 81.0 5 55.0 6 97.0 7 197.0 8 170.0 9 253.0 10 321.0 11 316.0 12 452.0 13 546.0 14 591.0 15 608.0 16 721.0 17 755.0 18 976.0 19 984.0 20 1167.0 21 1044.0 22 1378.0 23 1275.0 24 1450.0 25 1106.0 26 864.0 27 842.0 28 1065.0 29 1294.0 30 1580.0 31 1874.0 32 2350.0 33 2943.0 34 3368.0 35 3900.0 36 5116.0 37 7628.0 38 9344.0 39 8850.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 48.13284939786481 39.97175355738128 5.055505778371729 6.8398912663821765 11 31.945815426202373 44.198089568558366 14.540843444851099 9.31525156038816 12 66.06782183480388 14.458837643699981 11.400324985952711 8.07301553554344 13 18.25692113775456 25.76804507281811 24.52429042202615 31.450743367401174 14 24.55618156691825 46.773679175082385 18.073167398138164 10.596971859861197 15 39.33393065953925 16.354082825859162 28.77948032620085 15.532506188400735 16 15.543136570031436 20.39362784552537 47.64992634664156 16.413309237801638 17 40.68854500447995 14.896201916505946 13.409467114155113 31.005785964858994 18 36.58469903856377 18.460183174106533 21.41739698355078 23.537720803778917 19 20.720447090268497 15.707386708783865 50.59682906086746 12.975337140080184 20 20.140017312335797 35.17289556409361 21.772540205621954 22.914546917948638 21 19.705690291424318 23.216753481449985 39.58146668893985 17.49608953818585 22 24.935838053728986 31.62234809943963 11.666084526720223 31.77572932011116 23 28.062688879102186 48.56717641877629 13.442876884994456 9.927257817127064 24 18.68070111485768 50.01063215772047 17.61444758346244 13.694219143959415 25 19.842366626676185 27.45979437804674 23.830278364136127 28.867560631140943 26 38.28559278272227 22.26542328604842 23.44096473375657 16.008019197472738 27 22.66796732105841 16.757102792342398 23.683087428362395 36.8918424582368 28 26.62099971141724 40.25425659563481 18.6302951138383 14.494448579109646 29 41.558323207776425 19.845078979343864 27.943499392466585 10.653098420413123 30 22.91793313069909 39.171732522796354 27.767477203647417 10.142857142857142 31 37.60555251807302 26.140574691695523 26.58556588299617 9.668306907235284 32 24.374943038551507 27.39769723850898 38.730747030409816 9.496612692529697 33 32.820715436282406 22.20466218885816 24.77889554143999 20.195726833419446 34 23.54558852576082 24.036343194007628 30.352340580701036 22.065727699530516 35 20.287741366999377 39.49683241420172 29.021770505750272 11.193655713048631 36 27.21884498480243 31.18237082066869 24.185410334346503 17.41337386018237 37 17.583770230225667 28.54494339335917 39.25689537269205 14.61439100372312 38 20.876649036415586 28.331509514256187 36.14505441060247 14.646787038725758 39 19.785649133475218 30.065369413195498 30.673456977804804 19.475524475524473 40 17.013936382011885 33.593215702366294 38.30301372361282 11.089834192008997 41 17.337244828162763 33.143838635638176 38.05043396312453 11.468482573074526 42 22.606924643584524 33.44529896343131 33.08356385080706 10.864212542177098 43 15.768675431263773 35.71699977201915 33.49494642450034 15.019378372216735 44 22.561476032706164 32.887017842487616 36.22298550107906 8.328520623727165 45 21.60194543658333 32.60886085568812 35.134888669351774 10.654305038376776 46 17.335197823410496 33.92561292578012 34.84671155629189 13.892477694517488 47 20.476487312423032 35.30323993127879 32.71099083210436 11.509281924193818 48 23.121712426647207 32.99887500380066 31.980297363860256 11.899115205691873 49 22.4861248384399 34.88633771763096 31.735725689956663 10.891811753972478 50 16.570863746958636 35.49574209245742 33.66332116788321 14.27007299270073 51 19.522802962332154 35.68636992654998 29.78147477911769 15.009352332000184 52 19.603758666828853 35.828974577302034 33.9587641406155 10.608502615253618 53 12.270964798905194 35.02318862616893 38.14338934083479 14.562457234091081 54 15.923470008516851 37.04069838179827 30.969400170337025 16.066431439347852 55 22.402068913060013 32.422605917699855 32.3587130143759 12.816612154864227 56 17.603019051676913 38.290522855925495 32.42284983869986 11.683608253697729 57 20.702928870292887 35.40966146823887 29.483453784709013 14.403955876759225 58 20.40748010529359 31.504389768871444 31.40852999802194 16.67960012781303 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 90.0 7 180.0 8 178.0 9 152.5 10 129.0 11 121.5 12 114.0 13 173.0 14 232.0 15 308.5 16 380.5 17 376.0 18 503.5 19 631.0 20 714.0 21 797.0 22 1050.5 23 1781.5 24 2259.0 25 2827.0 26 3395.0 27 3798.0 28 3540.0 29 2879.0 30 3958.5 31 5038.0 32 3442.5 33 1847.0 34 1600.0 35 1334.0 36 1315.0 37 1224.5 38 1134.0 39 1164.0 40 1194.0 41 1880.0 42 3344.5 43 4123.0 44 3211.5 45 2300.0 46 2463.0 47 2761.5 48 2897.0 49 4473.0 50 6049.0 51 5044.0 52 4039.0 53 3481.5 54 2542.5 55 2161.0 56 1946.5 57 1732.0 58 1762.0 59 1625.5 60 1459.0 61 1231.5 62 1004.0 63 849.0 64 694.0 65 491.0 66 211.5 67 135.0 68 99.5 69 64.0 70 47.5 71 31.0 72 21.5 73 10.0 74 8.0 75 5.0 76 2.0 77 2.5 78 1.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.015186259472429346 19 0.0015186259472429347 20 0.0 21 0.0 22 0.0 23 0.0 24 0.016704885419672282 25 0.0 26 0.010630381630700542 27 0.36598885328554726 28 0.015186259472429346 29 0.01366763352518641 30 0.07441267141490379 31 0.007593129736214673 32 0.02277938920864402 33 0.06530091573144618 34 0.04859603031177391 35 0.0394842746283163 36 0.07441267141490379 37 0.06681954167868913 38 0.08048717520387554 39 0.1047851903597625 40 0.07593129736214672 41 0.09111755683457608 42 0.0835244270983614 43 0.08200580115111847 44 0.07744992330938966 45 0.08200580115111847 46 0.08808030494009021 47 0.11541557199046303 48 0.10782244225424836 49 0.1275645795684065 50 0.13515770930462118 51 0.13667633525186412 52 0.12300870172667769 53 0.1275645795684065 54 0.14730671688256466 55 0.17312335798569453 56 0.2019772509833103 57 0.18830961745812388 58 0.19438412124709564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 65849.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.9429907819405 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75096684626318 35.72111953104831 2 10.931147324232308 10.04419201506477 3 4.475589197765511 6.168658597700801 4 2.2840710012230194 4.1974821181794715 5 1.2825174362873104 2.946134337651293 6 0.7701715532343899 2.123039074245623 7 0.45945856609261887 1.4776230466673752 8 0.3602948467920537 1.324241825995839 9 0.2412983836313754 0.997737247338608 >10 1.2560737778071596 10.025968503697854 >50 0.0958582619905464 3.169372351896004 >100 0.0727200608204145 8.035049886862367 >500 0.0066109146200376825 2.2885693024951026 >1k 0.013221829240075365 11.480812161156585 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 2826 4.2916369269085335 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 2190 3.3257908244620262 No Hit AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1490 2.2627526613919726 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1054 1.6006317483940529 No Hit AAAGTTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 869 1.3196859481541101 No Hit AAAGTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 638 0.9688833543409923 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 490 0.744126714149038 No Hit AAAGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 482 0.7319777065710945 No Hit AAAGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 482 0.7319777065710945 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC 418 0.6347856459475466 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGTGG 397 0.6028945010554451 No Hit AAAGTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 326 0.4950720588011967 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC 276 0.41914076143905 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 240 0.3644702273383043 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGAAC 232 0.35232121976036085 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 199 0.302206563501344 Illumina Single End Adapter 2 (96% over 25bp) AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 186 0.2824644261871858 No Hit AAAGTTGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 177 0.2687967926619994 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 176 0.2672781667147565 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 156 0.2369056477698978 No Hit AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.23538702182265483 No Hit AAAGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 152 0.23083114398092605 No Hit AAAGTTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT 146 0.22171938829746843 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGAAGCTGAAAAACATCGAAC 137 0.20805175477228202 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 129 0.1959027471943386 No Hit AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115 0.17464198393293748 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 112 0.17008610609120867 Illumina Single End Adapter 2 (96% over 32bp) AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108 0.16401160230223694 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94 0.14275083904083585 No Hit AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAG 94 0.14275083904083585 No Hit AAAGTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG 87 0.1321204574101353 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAA 86 0.13060183146289236 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86 0.13060183146289236 No Hit AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 85 0.12908320551564945 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA 83 0.12604595362116355 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.12452732767392063 No Hit AAAGTTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG 82 0.12452732767392063 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 79 0.11997144983219184 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGGGG 76 0.11541557199046303 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.11541557199046303 No Hit AAAGTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGA 76 0.11541557199046303 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC 73 0.11085969414873421 No Hit AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 70 0.10630381630700543 No Hit AAAGTTGGGCAGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 70 0.10630381630700543 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT 69 0.1047851903597625 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC 69 0.1047851903597625 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG 67 0.10174793846527662 Illumina Single End Adapter 2 (96% over 30bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.07289404546766086 0.0 0.0 0.0 0.0 11 0.12149007577943477 0.0 0.0 0.0 0.0 12 0.12452732767392063 0.0 0.0 0.0 0.0 13 0.12908320551564945 0.0 0.0 0.0 0.0 14 0.16097435040775107 0.0 0.0 0.0 0.0 15 0.16401160230223694 0.0 0.0 0.0 0.0 16 0.16856748014396575 0.0 0.0 0.0 0.0 17 0.1716047320384516 0.0 0.0 0.0 0.0 18 0.17616060988018042 0.0 0.0 0.0 0.0 19 0.18223511366915215 0.0 0.0 0.0 0.0 20 0.2277938920864402 0.0 0.0 0.0 0.0 21 0.2369056477698978 0.0 0.0 0.0 0.0 22 0.25664778508405595 0.0 0.0 0.0 0.0 23 0.2930948078178864 0.0 0.0 0.0 0.0 24 0.3158741970265304 0.0 0.0 0.0 0.0 25 0.3204300748682592 0.0 0.0 0.0 0.0 26 0.33106045649895977 0.0 0.0 0.0 0.0 27 0.34017221218241733 0.0 0.0 0.0 0.0 28 0.4859603031177391 0.0 0.0 0.0 0.0 29 0.49051618095946786 0.0 0.0 0.0 0.0 30 0.5664474783216146 0.0 0.0 0.0 0.0 31 0.5816337377940439 0.0 0.0 0.0 0.0 32 0.5907454934775016 0.0 0.0 0.0 0.0 33 0.5922641194247444 0.0 0.0 0.0 0.0 34 0.6028945010554451 0.0 0.0 0.0 0.0 35 0.8762471715591733 0.0 0.0 0.0 0.0 36 0.8853589272426309 0.0 0.0 0.0 0.0 37 0.8929520569788455 0.0 0.0 0.0 0.0 38 0.9111755683457607 0.0 0.0 0.0 0.0 39 0.9187686980819755 0.0 0.0 0.0 0.0 40 0.929399079712676 0.0 0.0 0.0 0.0 41 0.9430667132378624 0.0 0.0 0.0 0.0 42 0.9643274764992634 0.0 0.0 0.0 0.0 43 0.9946999954441221 0.0 0.0 0.0 0.0 44 1.0114048808637945 0.0 0.0 0.0 0.0 45 1.044814651703139 0.0 0.0 0.0 0.0 46 1.0721499187535117 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACGCG 45 8.913048E-11 52.100536 22 AACGCGG 25 1.6194E-5 52.100533 23 AACGCCG 15 0.007135309 52.100533 23 TGCCGAA 15 0.007135309 52.100533 22 TGCGAAG 15 0.007135309 52.100533 25 CAACACA 20 3.391261E-4 52.100533 22 TCAACAC 25 1.6194E-5 52.100533 21 CAGAGAG 30 7.774979E-7 52.100533 27 CAGGGTC 25 1.6194E-5 52.100533 27 GATCCCA 15 0.007135309 52.100533 23 CGCAGGA 25 1.6194E-5 52.100533 25 CTGCCGA 15 0.007135309 52.100533 21 ACGCGGA 15 0.007135309 52.100533 24 ACGCGAT 15 0.007135309 52.100533 24 CAACGCC 15 0.007135309 52.100533 22 AGAAGTG 25 1.634081E-5 52.021294 51 GGCCGCT 105 0.0 52.021294 50 CCGCTCC 105 0.0 52.021294 52 TTCTCCT 105 0.0 52.021294 52 GCCGCTC 105 0.0 52.021294 51 >>END_MODULE