SRR212629_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212629_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60612
Sequences flagged as poor quality	0
Sequence length	56
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	2906	4.794430145845707	No Hit
AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1827	3.0142546030489012	No Hit
AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1291	2.129941265755956	No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1062	1.75212829142744	No Hit
AAAGTTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	721	1.189533425724279	No Hit
AAAGTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	556	0.9173101036098462	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGT	554	0.9140104269781562	No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	422	0.6962317692866099	No Hit
AAAGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	374	0.6170395301260476	No Hit
AAAGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	366	0.6038408235992874	No Hit
AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	238	0.39266151917112124	No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	234	0.38606216590774106	No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	186	0.3068699267471788	No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	171	0.2821223520095031	No Hit
AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGA	162	0.26727380716689764	No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	155	0.2557249389559823	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGG	151	0.24912558569260215	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	151	0.24912558569260215	Illumina Single End Adapter 2 (95% over 23bp)
AAAGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	145	0.23922655579753185	No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	142	0.2342770408499967	No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	138	0.2276776875866165	No Hit
AAAGTTGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	130	0.2144789810598561	No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	126	0.20787962779647595	No Hit
AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105	0.17323302316372996	No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	102	0.1682835082161948	No Hit
AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	101	0.16663366990034978	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	100	0.16498383158450472	No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.15673464000527948	No Hit
AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95	0.15673464000527948	No Hit
AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGAAGCTGAAAAACATCGA	88	0.14518577179436415	No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT	87	0.14353593347851912	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	83	0.1369365802151389	Illumina Single End Adapter 2 (96% over 30bp)
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTAAAGTTGGGAAGCAGT	83	0.1369365802151389	No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	80	0.13198706526760376	No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAA	80	0.13198706526760376	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	78	0.12868738863591367	No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA	70	0.1154886821091533	No Hit
AAAGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC	62	0.10228997558239293	No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.10064013726654789	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATGG	55	0.0	50.361137	50
GGCCGCT	20	4.007147E-4	50.361137	50
CCTTCTC	100	0.0	50.361134	50
AGGCCGC	45	1.2369128E-10	50.361134	49
AGCATCG	25	1.9776564E-5	50.361134	49
GTAGCAT	15	0.008156968	50.361134	49
CATCGAA	75	0.0	50.361134	50
GGGTAGG	15	0.0081839245	50.319164	47
GCAGGCC	45	1.2369128E-10	50.319164	47
CAGGCCG	45	1.2369128E-10	50.319164	48
AAAGCAT	25	1.9874537E-5	50.319164	47
AAGCATC	25	1.9874537E-5	50.319164	48
AGGGGGT	15	0.008210947	50.27727	45
GTCTGGT	15	0.008210947	50.27727	44
CGTAGCG	15	0.008210947	50.27727	41
CGTGCTT	15	0.008210947	50.27727	42
TGAAAAG	25	1.997291E-5	50.27727	44
GCGGGGC	20	4.04029E-4	50.277267	44
GAGCGGG	20	4.04029E-4	50.277267	42
CGGAGCG	45	1.2551027E-10	50.277267	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content