##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212629_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60612 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.13995578433313 38.0 36.0 39.0 33.0 40.0 2 36.88307595855606 38.0 36.0 39.0 32.0 40.0 3 36.72172177126642 38.0 36.0 39.0 31.0 40.0 4 36.56982115752656 38.0 35.0 39.0 31.0 40.0 5 35.756021909852834 38.0 35.0 39.0 29.0 40.0 6 37.05653995908401 38.0 36.0 40.0 33.0 40.0 7 36.96733320134627 38.0 36.0 39.0 33.0 40.0 8 36.70261664356893 38.0 36.0 40.0 31.0 40.0 9 36.46116280604501 38.0 36.0 40.0 30.0 40.0 10 35.794793110275194 38.0 35.0 39.0 29.0 40.0 11 35.83377878967861 38.0 35.0 39.0 29.0 40.0 12 35.78284828086848 38.0 35.0 39.0 29.0 40.0 13 35.488550122088036 38.0 35.0 39.0 29.0 40.0 14 35.646885105259685 38.0 35.0 39.0 29.0 40.0 15 35.714726456807234 38.0 35.0 39.0 29.0 40.0 16 35.307694845905104 38.0 35.0 39.0 28.0 40.0 17 35.40076222530192 38.0 35.0 39.0 29.0 40.0 18 35.194367451989706 38.0 35.0 39.0 28.0 40.0 19 34.567791856398074 38.0 34.0 39.0 26.0 40.0 20 34.908780439516924 38.0 35.0 39.0 27.0 40.0 21 34.72566488484129 38.0 35.0 39.0 26.0 40.0 22 34.527651290173566 38.0 35.0 39.0 26.0 40.0 23 34.08531313931235 38.0 33.0 39.0 25.0 40.0 24 33.92781957368178 38.0 33.0 39.0 24.0 40.0 25 33.92166567676368 38.0 33.0 39.0 24.0 40.0 26 33.19421896654128 38.0 33.0 39.0 19.0 40.0 27 33.29650894212367 38.0 33.0 39.0 19.0 40.0 28 32.269105127697486 37.0 32.0 39.0 10.0 40.0 29 32.46249917508084 38.0 33.0 39.0 2.0 40.0 30 31.58069359202798 37.0 31.0 39.0 2.0 40.0 31 30.481439318946745 36.0 30.0 39.0 2.0 40.0 32 29.373803867221014 35.0 26.0 39.0 2.0 40.0 33 29.868672870058735 36.0 28.0 39.0 2.0 40.0 34 29.907724542994785 36.0 29.0 39.0 2.0 40.0 35 28.802250379462812 35.0 26.0 39.0 2.0 40.0 36 27.78802877318023 35.0 17.0 39.0 2.0 40.0 37 27.064987131261137 35.0 2.0 39.0 2.0 40.0 38 26.991552827822872 35.0 2.0 39.0 2.0 40.0 39 26.991552827822872 35.0 2.0 39.0 2.0 40.0 40 26.581617501484853 35.0 2.0 39.0 2.0 40.0 41 26.570101630040256 35.0 2.0 39.0 2.0 40.0 42 26.618557381376625 35.0 2.0 39.0 2.0 40.0 43 26.197551639939284 35.0 2.0 39.0 2.0 40.0 44 26.064327195934798 35.0 2.0 39.0 2.0 40.0 45 26.051689434435424 35.0 2.0 39.0 2.0 40.0 46 25.838546822411402 35.0 2.0 39.0 2.0 40.0 47 25.533887679007456 35.0 2.0 39.0 2.0 40.0 48 25.555500560945028 35.0 2.0 39.0 2.0 40.0 49 25.367715963835543 35.0 2.0 39.0 2.0 40.0 50 25.067692866099122 35.0 2.0 39.0 2.0 40.0 51 24.53672540091071 35.0 2.0 39.0 2.0 40.0 52 24.174503398666932 34.0 2.0 39.0 2.0 40.0 53 23.8400976704283 34.0 2.0 39.0 2.0 40.0 54 23.573714775951956 33.0 2.0 39.0 2.0 40.0 55 23.04876922061638 33.0 2.0 39.0 2.0 40.0 56 13.075100640137267 16.0 2.0 22.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.9172006041455489 1 2 -1.3620208438954293 1 3 -1.6997643802456253 1 4 -1.5628301869212606 1 5 -2.616475175461048 1 6 -2.0805880333767064 1 7 -2.183777089774054 1 8 -1.7664797782505062 1 9 -1.8509218178915816 1 10 -3.1902266263052255 1 11 -3.4682719390215553 1 12 -4.785530121504447 1 13 -3.5855554772476523 1 14 -3.8496651394008197 1 15 -4.889797528928749 1 16 -5.270660969303993 1 17 -4.436592351670775 1 18 -4.8808235383561005 1 19 -5.786037042839453 1 20 -4.211162260571932 1 21 -5.018431058125955 1 22 -4.773000058497946 1 23 -5.752317050286084 1 24 -5.028875271124807 1 25 -5.40337469013328 1 26 -5.755961612485692 1 27 -5.326898484255278 1 28 -5.472167214079217 1 29 -4.773252531663871 1 30 -5.658814753925299 1 31 -5.155036452727902 1 32 -5.841407547170089 1 33 -5.713951949092216 1 34 -6.511362030789201 1 35 -5.518593364208741 1 36 -6.571138594935768 1 37 -5.303994640918127 1 38 -5.9359897795340295 1 39 -5.1131590648045275 1 40 -5.040103538166267 1 41 -4.135543015907675 1 42 -4.534633695488239 1 43 -4.882931388359594 1 44 -5.548216046656787 1 45 -4.916222583437051 1 46 -5.4668035615646176 1 47 -5.259379275214698 1 48 -5.475015548897602 1 49 -5.490266549512086 1 50 -5.190371571097593 1 51 -5.247443403004116 1 52 -5.392486031488382 1 53 -4.740996868856296 1 54 -5.669784518433698 1 55 -6.924604841455977 1 56 -3.578028249480761 2 1 -0.45478350760907205 2 2 -1.216110748095872 2 3 -1.736610364033389 2 4 -0.8686518523597329 2 5 -1.306968911643807 2 6 -1.1756506715491497 2 7 -1.0736075982486284 2 8 -1.6728909794295745 2 9 -2.104422186351414 2 10 -1.5671610404540885 2 11 -2.2040862352632686 2 12 -1.1083009394852894 2 13 -1.068237864867399 2 14 -1.0228412632033255 2 15 -1.941750366069499 2 16 -2.95415396856707 2 17 -1.4108001630193385 2 18 -2.44751476901196 2 19 -1.9301048394496192 2 20 -0.9488188560030295 2 21 -3.4167361428717165 2 22 -2.4063825198096644 2 23 -2.1274091652455525 2 24 -2.266900326393781 2 25 -2.5142811013344577 2 26 -1.8911863729867946 2 27 -1.2358889042994896 2 28 -3.4054759834233614 2 29 -3.9902753761738197 2 30 -3.18055388436008 2 31 -4.275891279551779 2 32 -3.206182786668986 2 33 -5.174158286601429 2 34 -5.0846855385268555 2 35 -5.225299333258114 2 36 -5.113511476291698 2 37 -5.212985060962339 2 38 -6.03068395786859 2 39 -5.418980730242993 2 40 -5.5765810621161584 2 41 -6.142175292989474 2 42 -5.871405987308432 2 43 -5.9043020589565 2 44 -6.600537343635416 2 45 -7.056605781668441 2 46 -7.47601505751156 2 47 -8.900867852959724 2 48 -8.286364111904234 2 49 -7.660494994611568 2 50 -7.331491689004739 2 51 -7.070214220984955 2 52 -5.816951764723459 2 53 -6.6076144075445775 2 54 -6.166468379892798 2 55 -6.386653478154578 2 56 -3.562184476452021 3 1 0.0881450836662836 3 2 -0.6064969664898712 3 3 0.23893839951203688 3 4 -0.3076626887031608 3 5 -0.1638736073926239 3 6 -0.04922666488062788 3 7 0.23425569711119465 3 8 0.2862643414929025 3 9 0.041451667496872346 3 10 -0.8277889926630309 3 11 -0.29827906660530346 3 12 0.38767016359890505 3 13 0.254073888397393 3 14 0.049123171361827644 3 15 -0.5480298881589718 3 16 -0.13559325725837823 3 17 -0.18712692045195922 3 18 0.06429406677575855 3 19 0.5992088587998907 3 20 0.3426509967338376 3 21 -0.02662777943743322 3 22 -0.25161730725061915 3 23 0.16072642797478665 3 24 -0.2163307237263723 3 25 -0.27115801158730335 3 26 0.6164546384052585 3 27 0.627128600562969 3 28 -1.720563507735669 3 29 -1.388719388399437 3 30 -2.1969473269830324 3 31 -3.127238876106375 3 32 -2.0580860931430074 3 33 -3.583160787296066 3 34 -3.974654974481286 3 35 -3.845749458292847 3 36 -3.390810726083309 3 37 -3.8253773921654535 3 38 -3.319097406048641 3 39 -3.317007960029045 3 40 -3.491279589484872 3 41 -3.757068318829983 3 42 -3.3984968458802562 3 43 -4.647008366264085 3 44 -3.7919794108763156 3 45 -3.5773059761669153 3 46 -3.25261966990389 3 47 -3.1442687976665873 3 48 -2.69953443699454 3 49 -2.4810006906382434 3 50 -2.7110415639700065 3 51 -2.3775218064253565 3 52 -2.120925090647326 3 53 -2.352543554529852 3 54 -1.703284162054512 3 55 -2.3935998521473536 3 56 -2.662490116907703 4 1 -0.11364329189258626 4 2 0.031655045432636086 4 3 -0.5551003486250679 4 4 -0.05848236083430436 4 5 -0.224380313800971 4 6 0.2166451682043231 4 7 0.5162229102259488 4 8 -0.07319914583943898 4 9 0.28109399238510235 4 10 0.08368641717303404 4 11 0.45939604516816956 4 12 -0.0993641135993073 4 13 0.10772515516341485 4 14 0.9266194455794192 4 15 0.0030878465653216836 4 16 0.17558408998454667 4 17 0.6203246443766588 4 18 0.7444729043316443 4 19 1.2230538662514547 4 20 0.404648016107906 4 21 -0.5002492399441394 4 22 -0.04058899130426852 4 23 -0.6574553902070335 4 24 -0.3972547177651222 4 25 -1.3626632276826882 4 26 -2.035408252801897 4 27 -1.7320368241762232 4 28 -2.542918202221511 4 29 -2.8700904763279027 4 30 -2.1441902479964448 4 31 -3.332902065376121 4 32 -2.3528699977629763 4 33 -2.3032800123333246 4 34 -2.237844244227933 4 35 -2.939937237726525 4 36 -2.8984113222084957 4 37 -2.4909511626572574 4 38 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0.24479433879737655 6 8 0.5099177372774477 6 9 0.5054446417357497 6 10 0.8765435600301785 6 11 1.3337466945188154 6 12 1.000960561725364 6 13 0.7979848954231556 6 14 0.761359705319677 6 15 1.6560099244873996 6 16 1.2798048692053285 6 17 -0.2673376932856826 6 18 0.4305118653706117 6 19 1.1068201000176927 6 20 0.38744022390011423 6 21 1.2494260847311907 6 22 1.367527891812614 6 23 0.48215499940335604 6 24 1.364758269247865 6 25 1.7581159930965313 6 26 2.3775787601851164 6 27 1.871859279719878 6 28 2.2522116679083624 6 29 1.4286108223733933 6 30 1.3908746870684894 6 31 2.597292739818684 6 32 1.95362350873053 6 33 1.6369797279264162 6 34 1.9319609505772597 6 35 2.9581147103254217 6 36 2.8038614050642323 6 37 3.1447631230570288 6 38 2.2441344440684574 6 39 2.7626173327110024 6 40 3.1501138531380803 6 41 3.5678731331606492 6 42 2.67732282625089 6 43 4.068155568162144 6 44 3.4711938912314118 6 45 2.7941811432710253 6 46 4.105401407379482 6 47 3.782546190623812 6 48 3.3262266871272423 6 49 3.974795562289156 6 50 3.485094267411725 6 51 3.1640472802256987 6 52 3.3047189374557178 6 53 3.314903752261717 6 54 3.9963645498892824 6 55 3.7586249722086187 6 56 1.6642077753639608 7 1 0.5489829707336753 7 2 0.7845169982945848 7 3 0.16577507225034793 7 4 0.46373986138791423 7 5 -0.09071028013093496 7 6 0.9210222725814248 7 7 0.7421488361518698 7 8 1.044309271668979 7 9 0.47435998565109116 7 10 1.3540567875434064 7 11 1.9230324088045307 7 12 1.3151140008788076 7 13 1.1326409800792447 7 14 1.1350369539969236 7 15 0.2704279139053867 7 16 0.4934292078296636 7 17 0.040863364915374234 7 18 0.8848769447356872 7 19 0.43618517938276824 7 20 0.9780486895085829 7 21 0.05564301594812093 7 22 -0.46382131453659525 7 23 0.9199856872340462 7 24 -0.9652681857785907 7 25 0.012084247064791498 7 26 0.24001262261897693 7 27 0.36392277178337196 7 28 -1.6267565860598943 7 29 -0.19837330461073321 7 30 -1.7916649954711907 7 31 -0.871622604096661 7 32 -1.5781225230155016 7 33 -1.5561419651952768 7 34 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19 0.8339629571605442 8 20 0.6210116524715446 8 21 0.6615689418740445 8 22 0.8739564632411856 8 23 0.8007264279747872 8 24 0.9954725549621486 8 25 1.0609731359536738 8 26 1.17186447447083 8 27 0.6661449940055917 8 28 0.624354525051217 8 29 0.7171822509448234 8 30 1.4594461156399205 8 31 -0.38199297446703184 8 32 0.4107663658733891 8 33 -0.40873455778787005 8 34 -0.8087533351370233 8 35 -1.029028146817435 8 36 -0.5511385949357681 8 37 -0.13095116265725437 8 38 -0.3472941273601151 8 39 -0.66881123871757 8 40 -0.8148861468619195 8 41 -1.016412581125067 8 42 -1.0676771737491109 8 43 0.1031555681621441 8 44 -1.2395203944828737 8 45 -1.6579617138718312 8 46 -1.3824557354776594 8 47 -1.9445966665190468 8 48 -1.7280590271584728 8 49 -1.3059187234251297 8 50 -1.0877628754454172 8 51 -1.8630955769171607 8 52 -1.1481382054014233 8 53 -1.3279533905954253 8 54 -0.5793497358250015 8 55 -2.2385178849342395 8 56 -1.3615065103503259 9 1 0.6759670977178018 9 2 -0.2657475519699659 9 3 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120 37 -0.8454966172027092 120 38 0.05906950900352115 120 39 -0.6724476023539339 120 40 0.025113853138080344 120 41 -0.07823076294324949 120 42 0.5523228262508901 120 43 0.6704282954348706 120 44 0.5150250600625803 120 45 0.9029473770372576 120 46 0.4966351736132495 120 47 0.7890396971173175 120 48 0.6573955182960738 120 49 0.24589945839305472 120 50 0.11496439728185592 120 51 -0.352186486008069 120 52 0.7982254309622121 120 53 1.1493193366773014 120 54 1.5288320823568142 120 55 1.060573024156671 120 56 1.2875843987405826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 192.0 3 33.0 4 50.0 5 53.0 6 113.0 7 455.0 8 262.0 9 388.0 10 536.0 11 392.0 12 473.0 13 665.0 14 644.0 15 703.0 16 732.0 17 780.0 18 858.0 19 1137.0 20 1259.0 21 1300.0 22 1156.0 23 1709.0 24 1721.0 25 1472.0 26 982.0 27 877.0 28 1004.0 29 1120.0 30 1345.0 31 1706.0 32 2030.0 33 2551.0 34 3058.0 35 3655.0 36 4794.0 37 7164.0 38 7628.0 39 5615.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 45.11482874678281 43.15152115092721 4.862073516795354 6.871576585494621 11 29.779581601003102 47.1688774500099 13.795947997096283 9.255592951890716 12 66.5214808948723 14.571372005543456 10.79654193888999 8.110605160694252 13 17.62852240480433 24.6667326601993 23.266019930046856 34.43872500494952 14 23.73787368837854 48.66033128753382 17.74236124859764 9.859433775490002 15 42.10882333531314 15.816999934006468 27.433511515871444 14.640665214808948 16 15.351745528938162 19.438395037286345 49.41925691282255 15.790602520952945 17 43.04428166039728 14.475681383224446 13.073318814756155 29.40671814162212 18 39.22325612090015 18.13172309113707 20.631228139642317 22.013792648320464 19 19.915198310565565 15.643766910842738 51.98970500890913 12.451329769682571 20 19.32125651686135 38.36369035834488 20.61637959479971 21.69867352999406 21 19.006137398534946 22.64733056160496 41.82835082161948 16.518181218240613 22 23.69497789216657 30.20359004817528 11.268395697221672 34.83303636243648 23 26.448558041311955 50.62693856002112 13.160760245495942 9.763743153170989 24 17.905695241866297 52.380716689764405 16.986735299940605 12.726852768428696 25 18.594059405940595 26.627062706270628 22.683168316831683 32.0957095709571 26 40.817033919286956 21.67698275150615 22.03680779070727 15.46917553849963 27 21.733170377221377 16.423333553544296 22.352513708132392 39.490982361101935 28 24.988850899360784 42.625902251292466 18.011991477131957 14.373255372214789 29 43.77581705825981 19.244241763325736 26.476322659528766 10.503618518885695 30 21.785631632889213 41.427248983370255 26.746454193804343 10.040665189936192 31 39.92130150122347 24.960320084650487 25.590238740824017 9.52813967330203 32 24.607901528687712 26.46416517768513 39.44642975315995 9.481503540467209 33 34.108437324153456 21.449141041342557 24.47783919764324 19.964582436860745 34 22.95825470917337 22.822524580395275 29.902009468004103 24.317211242427252 35 19.131428192986522 41.597403887545944 28.580416570085106 10.69075134938243 36 28.321794149799583 30.011263126511412 23.566435882995993 18.100506840693015 37 15.745174384889404 27.788915582801753 40.54179438323254 15.924115649076299 38 23.16755878892332 26.8713852476675 34.73310905987439 15.227946903534791 39 18.96800278380752 28.51414273641651 30.409783094997433 22.108071384778537 40 16.465204198378352 31.645359730720124 40.103467144207336 11.785968926694192 41 16.72442668346958 34.08891173495614 37.887807385543965 11.29885419603031 42 21.456428298786864 34.67149044924241 33.177888046202106 10.694193205768626 43 16.141987089065534 36.100831411075525 33.372608241092614 14.384573258766325 44 25.47666063753879 30.312131822179445 35.75494084263976 8.456266697642002 45 21.323370693233706 31.11000415110004 37.418015774180155 10.148609381486095 46 16.76685486549319 31.876453005645967 37.87944204583195 13.477250083028894 47 23.19346157679657 32.6456028439483 32.609056779294995 11.551878799960132 48 25.400149588631265 31.01470954874096 31.84908169201363 11.736059170614146 49 24.89784392545098 32.40922228497392 31.756420052489954 10.936513737085146 50 16.185806880505236 35.86671098554097 34.37593485125478 13.57154728269902 51 18.62722286853914 36.12099052684062 30.806049526342033 14.445737078278212 52 21.87988427388514 33.542283262944366 34.250606897010414 10.327225566160088 53 12.360316615671145 33.27790341891712 37.6579752560862 16.703804709325528 54 15.144254766896276 37.100262886426414 31.53805197830355 16.217430368373766 55 23.714100410756156 31.227445828413686 32.61936041774067 12.439093343089485 56 17.991019457841343 36.715449858639616 32.80558789289872 12.487942790620323 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 81.0 7 162.0 8 163.0 9 164.0 10 141.5 11 119.0 12 112.0 13 163.5 14 222.0 15 296.0 16 370.0 17 365.0 18 360.0 19 489.5 20 619.0 21 719.0 22 1066.5 23 1314.0 24 1767.5 25 2221.0 26 2789.5 27 3358.0 28 3696.5 29 3306.5 30 2578.0 31 3767.0 32 4956.0 33 3321.0 34 1686.0 35 1454.0 36 1222.0 37 1162.0 38 1060.0 39 1018.0 40 1499.5 41 1981.0 42 2696.0 43 3411.0 44 2695.0 45 1979.0 46 2000.5 47 2987.5 48 3953.0 49 4568.5 50 5184.0 51 4602.0 52 4020.0 53 3470.0 54 2570.5 55 2221.0 56 1897.5 57 1574.0 58 1626.5 59 1679.0 60 1453.0 61 1227.0 62 941.0 63 597.0 64 539.0 65 497.0 66 455.0 67 332.5 68 210.0 69 153.0 70 96.0 71 60.5 72 21.0 73 17.0 74 11.5 75 6.0 76 4.0 77 2.0 78 2.0 79 2.0 80 2.0 81 1.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.019798059790140567 26 0.04454563452781627 27 0.10558965221408301 28 0.11383884379330825 29 0.14848544842605427 30 0.1946809212697156 31 0.21117930442816601 32 0.27717283706196794 33 0.31346928001055896 34 0.3266679865373193 35 0.3530653995908401 36 0.39266151917112124 37 0.4240084471721771 38 0.44380650696231766 39 0.43390747706724736 40 0.49990100970104934 41 0.5032006863327394 42 0.5856926021249917 43 0.5823929254933017 44 0.5757935722299214 45 0.6384874282320332 46 0.6467366198112585 47 0.6830330627598495 48 0.7374777271827361 49 0.6764337094964694 50 0.7292285356035109 51 0.7292285356035109 52 0.7754240084471722 53 0.7853230383422425 54 0.841417541080974 55 0.7902725532897775 56 0.7952220682373128 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 60612.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.09278690688313 #Duplication Level Percentage of deduplicated Percentage of total 1 78.45283018867924 37.730152445060384 2 10.981132075471699 10.562264898039993 3 4.264150943396226 6.15224707978618 4 2.0891938250428814 4.019006137398535 5 1.2246998284734134 2.944961393783409 6 0.6792452830188679 1.9600079192239162 7 0.476843910806175 1.605292681317231 8 0.3053173241852487 1.1746848808816737 9 0.25042881646655235 1.083943773510196 >10 1.118353344768439 9.719197518643172 >50 0.06861063464837049 2.3988649112386984 >100 0.06518010291595197 5.937768098726324 >500 0.010291595197255574 3.0208539563122816 >1k 0.0137221269296741 11.690754306078006 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 2906 4.794430145845707 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1827 3.0142546030489012 No Hit AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1291 2.129941265755956 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 1062 1.75212829142744 No Hit AAAGTTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 721 1.189533425724279 No Hit AAAGTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 556 0.9173101036098462 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGT 554 0.9140104269781562 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 422 0.6962317692866099 No Hit AAAGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 374 0.6170395301260476 No Hit AAAGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 366 0.6038408235992874 No Hit AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 238 0.39266151917112124 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 234 0.38606216590774106 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC 186 0.3068699267471788 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 171 0.2821223520095031 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGA 162 0.26727380716689764 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.2557249389559823 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGG 151 0.24912558569260215 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 151 0.24912558569260215 Illumina Single End Adapter 2 (95% over 23bp) AAAGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 145 0.23922655579753185 No Hit AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 142 0.2342770408499967 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC 138 0.2276776875866165 No Hit AAAGTTGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 130 0.2144789810598561 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.20787962779647595 No Hit AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 105 0.17323302316372996 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.1682835082161948 No Hit AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.16663366990034978 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA 100 0.16498383158450472 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 95 0.15673464000527948 No Hit AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 0.15673464000527948 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGAAGCTGAAAAACATCGA 88 0.14518577179436415 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT 87 0.14353593347851912 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 83 0.1369365802151389 Illumina Single End Adapter 2 (96% over 30bp) AAAGTTGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTAAAGTTGGGAAGCAGT 83 0.1369365802151389 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.13198706526760376 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAA 80 0.13198706526760376 No Hit AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 78 0.12868738863591367 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 70 0.1154886821091533 No Hit AAAGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 62 0.10228997558239293 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.10064013726654789 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.05774434105457665 0.0 0.0 0.0 0.0 11 0.10888932884577311 0.0 0.0 0.0 0.0 12 0.11383884379330826 0.0 0.0 0.0 0.0 13 0.12043819705668844 0.0 0.0 0.0 0.0 14 0.1501352867418993 0.0 0.0 0.0 0.0 15 0.15178512505774433 0.0 0.0 0.0 0.0 16 0.16168415495281463 0.0 0.0 0.0 0.0 17 0.16663366990034978 0.0 0.0 0.0 0.0 18 0.1715831848478849 0.0 0.0 0.0 0.0 19 0.174882861479575 0.0 0.0 0.0 0.0 20 0.2029301128489408 0.0 0.0 0.0 0.0 21 0.21447898105985613 0.0 0.0 0.0 0.0 22 0.24252623242922194 0.0 0.0 0.0 0.0 23 0.2639741305352076 0.0 0.0 0.0 0.0 24 0.2821223520095031 0.0 0.0 0.0 0.0 25 0.2936712202204184 0.0 0.0 0.0 0.0 26 0.29862073516795357 0.0 0.0 0.0 0.0 27 0.30522008843133375 0.0 0.0 0.0 0.0 28 0.4520556985415429 0.0 0.0 0.0 0.0 29 0.4685540816999934 0.0 0.0 0.0 0.0 30 0.5444466442288656 0.0 0.0 0.0 0.0 31 0.5559955124397808 0.0 0.0 0.0 0.0 32 0.5642447040190062 0.0 0.0 0.0 0.0 33 0.5724938955982314 0.0 0.0 0.0 0.0 34 0.5823929254933017 0.0 0.0 0.0 0.0 35 0.805121098132383 0.0 0.0 0.0 0.0 36 0.8265689962383687 0.0 0.0 0.0 0.0 37 0.8348181878175939 0.0 0.0 0.0 0.0 38 0.8447172177126642 0.0 0.0 0.0 0.0 39 0.8513165709760443 0.0 0.0 0.0 0.0 40 0.8612156008711146 0.0 0.0 0.0 0.0 41 0.8711146307661849 0.0 0.0 0.0 0.0 42 0.8876130139246354 0.0 0.0 0.0 0.0 43 0.9041113970830859 0.0 0.0 0.0 0.0 44 0.9239094568732265 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATATGG 55 0.0 50.361137 50 GGCCGCT 20 4.007147E-4 50.361137 50 CCTTCTC 100 0.0 50.361134 50 AGGCCGC 45 1.2369128E-10 50.361134 49 AGCATCG 25 1.9776564E-5 50.361134 49 GTAGCAT 15 0.008156968 50.361134 49 CATCGAA 75 0.0 50.361134 50 GGGTAGG 15 0.0081839245 50.319164 47 GCAGGCC 45 1.2369128E-10 50.319164 47 CAGGCCG 45 1.2369128E-10 50.319164 48 AAAGCAT 25 1.9874537E-5 50.319164 47 AAGCATC 25 1.9874537E-5 50.319164 48 AGGGGGT 15 0.008210947 50.27727 45 GTCTGGT 15 0.008210947 50.27727 44 CGTAGCG 15 0.008210947 50.27727 41 CGTGCTT 15 0.008210947 50.27727 42 TGAAAAG 25 1.997291E-5 50.27727 44 GCGGGGC 20 4.04029E-4 50.277267 44 GAGCGGG 20 4.04029E-4 50.277267 42 CGGAGCG 45 1.2551027E-10 50.277267 41 >>END_MODULE