FastQCFastQC Report
Tue 31 May 2016
SRR212628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52289
Sequences flagged as poor quality0
Sequence length56
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT24984.777295415861844No Hit
AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA15512.9662070416339956No Hit
AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA12192.331274264185584No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA9471.811088374227849No Hit
AAAGTTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA5771.1034825680353422No Hit
AAAGTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4610.8816385855533669No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA4150.7936659718105147No Hit
AAAGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3600.6884813249440609No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGT3410.6521448105720132No Hit
AAAGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3220.6158082961999656No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA2530.4838493755856872No Hit
AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2140.4092638987167473No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1840.3518904549714089No Hit
AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGA1750.3346784218478074No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA1670.31937883684905044No Hit
AAAGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1280.24479335998011054No Hit
AAAGTTGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1270.24288091185526592No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC1270.24288091185526592No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG1260.2409684637304213Illumina Single End Adapter 2 (95% over 23bp)
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.2371435674807321No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGG1210.23140622310619824No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.22566887873166439No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.2103692937329075No Hit
AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.18933236435961673No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.1835950199850829No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA960.1835950199850829No Hit
AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.18168257186023828No Hit
AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT920.17594522748570446No Hit
AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.15682074623725833No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA800.1529958499875691No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA800.1529958499875691No Hit
AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGAAGCTGAAAAACATCGA760.14534605748819063No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG720.13769626498881218Illumina Single End Adapter 2 (96% over 30bp)
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAA710.13578381686396757No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTAAAGTTGGGAAGCAGT700.13387136873912295No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA590.11283443936583219No Hit
AAAGTTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG590.11283443936583219No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAA580.11092199124098757No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA560.10709709499129837No Hit
AAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10518464686645375No Hit
AAAGTTGGGCAGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAA540.10327219874160913No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCGGTGGGAAGGGCGGGTAGGCCG530.10135975061676451No Hit
AAAGTTGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG530.10135975061676451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATGG204.033138E-450.28212750
TTTTATG150.00820143150.28212750
CCTTCTC1000.050.28212750
GCATCGA204.033138E-450.28212750
CATCGAA800.050.28212750
GAGCGTG900.050.23359342
ACCGGGG150.0082328450.23359345
CACCGGG150.0082328450.23359344
AGAGGGG204.0524383E-450.23359343
GAAAAGC150.0082328450.23359345
CGTAGCG600.050.23359341
CATCACC150.0082328450.23359341
AAAAGCA150.0082328450.23359346
CGGGAAG204.0524383E-450.23359346
ATCACCG150.0082328450.23359342
AGCATCG150.0082328450.23359349
TCACCGG150.0082328450.23359343
CCGGGGC150.0082328450.23359346
GGTATGC204.0524383E-450.23359348
GGAGCGT900.050.23359341
Produced by FastQC (version 0.11.5)