##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212628_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52289 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.55332861596129 39.0 38.0 40.0 33.0 40.0 2 37.31287651322458 39.0 37.0 40.0 33.0 40.0 3 37.15226911970013 39.0 37.0 40.0 33.0 40.0 4 37.035131672053396 38.0 36.0 40.0 33.0 40.0 5 36.421331446384514 39.0 35.0 39.0 30.0 40.0 6 37.4234160148406 39.0 38.0 40.0 33.0 40.0 7 37.29853315228824 38.0 38.0 40.0 33.0 40.0 8 37.09380558052363 38.0 37.0 40.0 33.0 40.0 9 36.89001510834019 38.0 37.0 40.0 32.0 40.0 10 36.26516093250971 38.0 35.0 39.0 30.0 40.0 11 36.32064105261145 38.0 36.0 40.0 31.0 40.0 12 36.269138824609385 38.0 35.0 40.0 31.0 40.0 13 36.02599017001664 38.0 35.0 40.0 30.0 40.0 14 36.239916617261756 38.0 35.0 40.0 30.0 40.0 15 36.26189064621622 38.0 36.0 40.0 31.0 40.0 16 35.962592514678036 38.0 36.0 40.0 30.0 40.0 17 36.01682954349863 38.0 36.0 40.0 30.0 40.0 18 35.82436076421427 38.0 35.0 40.0 29.0 40.0 19 35.363766757826696 38.0 35.0 40.0 29.0 40.0 20 35.61764424639981 38.0 35.0 40.0 29.0 40.0 21 35.36407274952667 38.0 35.0 40.0 29.0 40.0 22 35.19893285394634 38.0 35.0 40.0 28.0 40.0 23 34.76649008395647 38.0 35.0 39.0 27.0 40.0 24 34.65935474000268 38.0 35.0 39.0 27.0 40.0 25 34.706152345617625 38.0 35.0 39.0 27.0 40.0 26 33.93088412476811 38.0 34.0 39.0 23.0 40.0 27 34.00166382986861 38.0 35.0 39.0 23.0 40.0 28 33.106618982960086 38.0 33.0 39.0 18.0 40.0 29 33.266901260303314 38.0 33.0 39.0 18.0 40.0 30 32.49245539214749 38.0 33.0 39.0 2.0 40.0 31 31.441565147545372 37.0 31.0 39.0 2.0 40.0 32 30.35607871636482 36.0 29.0 39.0 2.0 40.0 33 30.937558568723823 37.0 30.0 39.0 2.0 40.0 34 30.983093958576372 37.0 30.0 39.0 2.0 40.0 35 29.8738740461665 36.0 29.0 39.0 2.0 40.0 36 28.918873950544093 36.0 25.0 39.0 2.0 40.0 37 28.143835223469562 36.0 20.0 39.0 2.0 40.0 38 28.088814090917783 36.0 18.0 39.0 2.0 40.0 39 28.067146053663294 36.0 18.0 39.0 2.0 40.0 40 27.635028399854654 35.0 16.0 39.0 2.0 40.0 41 27.63026640402379 35.0 8.0 39.0 2.0 40.0 42 27.646082350016258 36.0 2.0 39.0 2.0 40.0 43 27.258486488554 35.0 2.0 39.0 2.0 40.0 44 27.057373443745337 35.0 2.0 39.0 2.0 40.0 45 27.03901394174683 35.0 2.0 39.0 2.0 40.0 46 26.76974124576871 35.0 2.0 39.0 2.0 40.0 47 26.459924649543883 35.0 2.0 39.0 2.0 40.0 48 26.50941880701486 35.0 2.0 39.0 2.0 40.0 49 26.31457859205569 35.0 2.0 39.0 2.0 40.0 50 25.98575226146991 35.0 2.0 39.0 2.0 40.0 51 25.39285509380558 35.0 2.0 39.0 2.0 40.0 52 25.026544779972845 35.0 2.0 39.0 2.0 40.0 53 24.59580408881409 34.0 2.0 39.0 2.0 40.0 54 24.378779475606724 34.0 2.0 39.0 2.0 40.0 55 23.773833884755877 34.0 2.0 39.0 2.0 40.0 56 13.442616994014037 17.0 2.0 22.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.6200136702479213 1 2 0.6305002426194406 1 3 0.7482248443551427 1 4 0.5290730285997185 1 5 0.40138379915229905 1 6 0.31871728109791064 1 7 0.17559223207297237 1 8 0.2637232417668116 1 9 0.2684276683169813 1 10 0.2604017321514718 1 11 -0.025992563226807874 1 12 0.017294389951985067 1 13 0.5149573190129502 1 14 0.39846669579291216 1 15 0.12743098156774835 1 16 0.5329534270095095 1 17 0.7335765262128504 1 18 0.24481622742286646 1 19 -0.09939828901175218 1 20 1.3906686674363087 1 21 1.1947348574172452 1 22 0.7232768196345987 1 23 1.2857856210632264 1 24 1.1861162232005356 1 25 1.2211353774203317 1 26 1.8654242599631843 1 27 1.7526597356828333 1 28 1.4232731772815157 1 29 2.1138543502318257 1 30 0.8317661281839364 1 31 -0.6347424479648147 1 32 -1.7559037373216597 1 33 -1.098371730531369 1 34 -1.5049926177516184 1 35 -2.180730238966394 1 36 -2.0280264471861607 1 37 -1.4299677173294398 1 38 -1.1576755281811977 1 39 -1.9234621008961525 1 40 -1.53698707702263 1 41 -1.715269699661313 1 42 -1.1771181973708202 1 43 -1.6221146210782784 1 44 -1.1704031914450361 1 45 -1.814048180938098 1 46 -1.5370866106415058 1 47 -1.3810853521051847 1 48 -1.6547863971494436 1 49 -1.2563406068050718 1 50 -0.9235644497151867 1 51 -0.0053863717678765966 1 52 0.42564109932551375 1 53 0.5332079921804187 1 54 0.587813536755398 1 55 1.1032065555107948 1 56 1.971708507158045 2 1 -0.5199863297520793 2 2 -0.8981954095544751 2 3 -1.0613403730361597 2 4 -0.5709269714002829 2 5 -1.3333988095433469 2 6 -1.3795435884673068 2 7 -0.9383208114052835 2 8 -0.6214941495375399 2 9 -1.1507027664656277 2 10 -1.4917721808920064 2 11 -1.717296911052891 2 12 -1.6992273491784502 2 13 -1.8798252896826995 2 14 -2.3102289563810032 2 15 -1.7943081488670316 2 16 -1.321829181686141 2 17 -0.9681626042219307 2 18 -0.802140294316267 2 19 -0.8646156803161062 2 20 -0.022374810824558722 2 21 -0.7304825338871055 2 22 -0.7088970934088792 2 23 -1.291605683284601 2 24 -1.1877968202777254 2 25 -0.9875602747535837 2 26 -1.1806626965585565 2 27 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1.0938138335209295 4 39 1.6148357714442731 4 40 1.6408852634029003 4 41 -0.35356757200174016 4 42 0.17649882390577787 4 43 -0.30211462107827813 4 44 0.5704478723847508 4 45 -0.37192052136363074 4 46 -0.6481504404287399 4 47 -0.9478938627434843 4 48 -1.1041480992771042 4 49 -0.816766138719963 4 50 -0.909521896523696 4 51 -1.5892161590019178 4 52 -1.2407418793978877 4 53 -2.2399834971812815 4 54 -1.2994205057977943 4 55 -0.9857296147019738 4 56 -0.21680213113982738 5 1 -0.5927136024793498 5 2 -0.3258633937441999 5 3 -1.2826842465539485 5 4 -0.5800178804911909 5 5 -0.47134347357496864 5 6 -0.8558281734475415 5 7 -0.6462259497452081 5 8 -0.6017313036877354 5 9 -0.6024814225921062 5 10 -0.9214164496667081 5 11 -0.8532652904995288 5 12 -1.4790692464116475 5 13 -0.7995881355325025 5 14 -0.6197151223889108 5 15 -0.49075083661406893 5 16 -0.5415920275359483 5 17 -0.7282416556053306 5 18 0.26663440924104975 5 19 0.0824198928064277 5 20 0.13612321289085116 5 21 0.7983712210536069 5 22 0.8160040923618723 5 23 0.46214925742686574 5 24 0.5224798595641715 5 25 0.2756808319657864 5 26 0.2726969872359106 5 27 -1.5037039006807973 5 28 -0.02399954999120979 5 29 0.7774907138681897 5 30 1.2863115827293896 5 31 0.9725302793079145 5 32 -0.335903737321658 5 33 -0.43109900325864103 5 34 -0.1577198904788908 5 35 0.5192697610336054 5 36 0.13924628008656725 5 37 1.4318504644887433 5 38 0.6477790172733471 5 39 0.4492651718311187 5 40 1.015740195704641 5 41 1.193821209429597 5 42 1.1556090753564519 5 43 0.47970356073990317 5 44 1.1077786267367813 5 45 0.9441336372437199 5 46 0.5483679348130366 5 47 -0.39199444301427633 5 48 -1.1602409426039912 5 49 -1.0399769704414332 5 50 -0.9617462678970021 5 51 -0.28902273540423806 5 52 -0.970722537038121 5 53 -2.486792007819581 5 54 -1.4394591905173293 5 55 -1.8367934444892065 5 56 -0.9901096746601379 6 1 -0.45748632975207926 6 2 -0.4144997573805611 6 3 -0.6084418223115193 6 4 -0.19592697140028292 6 5 -0.6902828675143624 6 6 -0.17211605223542392 6 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21 1.0222348574172457 117 22 1.2766101529679332 117 23 1.4049522877298912 117 24 1.8611162232005327 117 25 1.4336353774203303 117 26 0.8329242599631854 117 27 1.2784930690161715 117 28 1.8324398439481797 117 29 2.147187683565164 117 30 2.090099461517269 117 31 2.728590885368522 117 32 1.976596262678342 117 33 2.345794936135295 117 34 2.3025073822483826 117 35 2.598436427700271 117 36 -0.9671931138528294 117 37 0.5375322826705613 117 38 0.4439911384854689 117 39 -0.002628767562821821 117 40 -1.6986537436892988 117 41 -0.590269699661313 117 42 -0.38378486403748724 117 43 0.21871871225505402 117 44 -0.5479031914450374 117 45 0.1134518190619005 117 46 0.5362467226918248 117 47 0.6864146478948143 117 48 -0.1772863971494445 117 49 0.08615939319492938 117 50 0.16893555028481444 117 51 0.03711362823212383 117 52 -0.571858900674485 117 53 -1.103458674486248 117 54 0.6753135367553966 117 55 -0.5451267778225386 117 56 -0.574958159508622 118 1 -1.1866529964187436 118 2 -1.0149899534589935 118 3 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118 41 1.5396322611229998 118 42 1.1456269006683968 118 43 1.7371010651962315 118 44 0.8895968085549626 118 45 1.2396773092579778 118 46 1.5362467226918248 118 47 2.1067577851497177 118 48 1.973448896968204 118 49 1.6609142951557132 118 50 2.6015335895005016 118 51 3.474613628232124 118 52 2.1793665895215923 118 53 2.4504628941412037 118 54 0.9584017720495126 118 55 1.1632065555107935 118 56 1.5917085071580441 119 1 -0.030190411384729998 119 2 0.24111248751739822 119 3 0.1959799463959584 119 4 -0.009702481604364266 119 5 0.1728123705808713 119 6 0.272186668853017 119 7 0.44987794635868994 119 8 0.2906620172770147 119 9 0.3129174642353476 119 10 0.6399935688861618 119 11 -0.9586456244512931 119 12 -1.018215814129647 119 13 -1.8225937013952134 119 14 -1.9770435082887232 119 15 -1.0888955490444943 119 16 -2.989087389317021 119 17 -1.7015255146034747 119 18 -2.225387854209785 119 19 -1.7279697175831856 119 20 -1.7521884754208372 119 21 -0.9815916731949983 119 22 -1.8322333844470364 119 23 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-1.3552696996613136 120 42 -1.237118197370819 120 43 -1.0021146210782774 120 44 -1.1904031914450357 120 45 -2.654048180938098 120 46 -1.7970866106415073 120 47 -1.2810853521051868 120 48 -1.1747863971494432 120 49 -1.8563406068050696 120 50 -2.4235644497151867 120 51 -1.5453863717678757 120 52 -1.6743589006744841 120 53 -1.7667920078195785 120 54 -2.692186463244603 120 55 -1.4567934444892074 120 56 -2.2882914928419567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 145.0 3 27.0 4 43.0 5 47.0 6 80.0 7 309.0 8 202.0 9 259.0 10 368.0 11 274.0 12 347.0 13 502.0 14 543.0 15 491.0 16 525.0 17 648.0 18 680.0 19 825.0 20 879.0 21 1048.0 22 854.0 23 1421.0 24 1458.0 25 1310.0 26 822.0 27 650.0 28 743.0 29 849.0 30 1078.0 31 1380.0 32 1636.0 33 2145.0 34 2557.0 35 3094.0 36 4102.0 37 6450.0 38 7493.0 39 6005.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 46.87984088431602 41.19413260915298 5.085199563961827 6.840826942569183 11 30.99887165560634 45.10126412821052 14.458107823825278 9.441756392357856 12 65.93738644839259 14.526955956319684 11.226070492837882 8.309587102449846 13 18.434087475377233 25.10661898296009 24.347377077396775 32.1119164642659 14 24.38562603989367 47.17244544741724 18.070722331656754 10.37120618103234 15 40.34118074547228 16.487215284285416 27.91026793398229 15.261336036260017 16 15.41241943812274 20.42303352521563 47.774866606743295 16.38968042991834 17 41.35477825163992 15.035667157528351 13.43494807703341 30.174606513798313 18 37.396010633211574 18.808927307846773 20.84950945705598 22.945552601885673 19 20.39052190709327 15.844632714337623 50.80227198837232 12.962573390196791 20 20.161028132111916 36.02861022394768 21.241561322649122 22.568800321291285 21 19.547132284036795 22.945552601885673 40.04857618237105 17.458738931706478 22 24.332542839657282 31.35136168910649 11.849755201958384 32.46634026927784 23 27.860230271965726 49.08197222965995 13.078070611636003 9.979726886738325 24 18.42452523475301 50.85964543211765 17.661458432940005 13.054370900189333 25 19.65372106370767 26.967667878324086 23.619667113066768 29.75894394490147 26 38.72987306388926 22.06161092071758 23.27162030211943 15.936895713273728 27 22.203285971429665 16.682624181379495 23.637547393818696 37.47654245337214 28 26.42907753882686 40.40100346617132 18.510503839598613 14.659415155403204 29 42.015212767995706 19.927959688080776 27.223957235644626 10.832870308278888 30 22.828650877361202 39.463035765653466 27.174225716751366 10.534087640233963 31 38.12336964861132 25.75187969924812 26.53444836581249 9.590302286328065 32 24.709809865505267 27.386274246464957 38.61015713436043 9.293758753669348 33 32.6187505997505 22.514154111889454 24.911236925439017 19.955858362921024 34 22.77254322503886 23.89130893669283 30.31797509163133 23.018172746636985 35 19.23128611745733 40.47267072398103 29.43536775011039 10.860675408451245 36 26.825613340761944 30.975389521815146 24.030277996580278 18.168719140842633 37 15.79361726640808 28.495717961519258 39.14128806789815 16.569376704174505 38 21.314909482261598 28.102778952223545 34.915632086712534 15.666679478802322 39 18.931441534971356 29.49590494866767 30.284154266159113 21.28849925020187 40 16.729833201869987 32.8498047288328 38.465534158025356 11.95482791127186 41 16.83629297573927 34.00157762087077 37.95140158146872 11.210727821921235 42 21.720467363477123 34.08596562145098 33.50657350195376 10.68699351311813 43 16.589977089389883 35.62503609865039 33.16262682659171 14.622359985368014 44 24.272293238872635 30.67919297705221 36.333359002002155 8.715154782073002 45 21.4855184101063 32.16954244338315 36.02102911723926 10.3239100292713 46 17.3024706811223 32.83906872845616 35.97411850796279 13.884342082458742 47 21.901128963896273 33.86699032867106 32.44711594035372 11.78476476707895 48 24.15866774603909 32.095909949500786 31.893527620369298 11.851894684090821 49 23.34014103502755 33.51893953990212 31.85426380486301 11.286655620207314 50 16.467798831852264 35.69982844034929 33.82038282861384 14.01198989918461 51 19.211499922922766 35.74263912440265 30.420456297209803 14.625404655464777 52 21.19710843373494 34.5310843373494 33.661686746987954 10.61012048192771 53 12.500481974166187 34.168112589165226 37.499518025833815 15.83188741083478 54 15.847826925301762 37.4474567120435 30.972581080559948 15.73213528209479 55 23.135983949688445 32.081331867199104 32.2182996701198 12.56438451299265 56 18.38328897118389 37.66539366585658 31.483624580488367 12.467692782471165 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 73.0 7 146.0 8 144.0 9 142.0 10 126.0 11 110.0 12 99.0 13 133.5 14 179.0 15 249.0 16 319.0 17 296.0 18 273.0 19 390.5 20 508.0 21 633.5 22 966.0 23 1173.0 24 1558.5 25 1944.0 26 2527.5 27 3111.0 28 3353.0 29 2968.0 30 2341.0 31 3303.5 32 4266.0 33 2816.0 34 1366.0 35 1214.5 36 1063.0 37 974.0 38 907.0 39 929.0 40 1281.0 41 1633.0 42 2234.0 43 2835.0 44 2295.0 45 1755.0 46 1727.0 47 2464.0 48 3229.0 49 3842.5 50 4456.0 51 3889.0 52 3322.0 53 2927.5 54 2224.0 55 1915.0 56 1653.5 57 1392.0 58 1396.5 59 1401.0 60 1278.0 61 1155.0 62 855.0 63 526.0 64 497.0 65 438.0 66 379.0 67 290.5 68 202.0 69 139.0 70 76.0 71 53.0 72 21.0 73 12.0 74 8.5 75 5.0 76 5.5 77 6.0 78 4.5 79 2.0 80 1.0 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0019124481248446136 23 0.005737344374533841 24 0.0 25 0.03633651437204766 26 0.11092199124098757 27 0.12813402436458912 28 0.13387136873912295 29 0.1835950199850829 30 0.27539252997762437 31 0.2926045631012259 32 0.3212912849738951 33 0.3518904549714089 34 0.3404157662223412 35 0.38631452121861193 36 0.455162653713018 37 0.4035265543422135 38 0.4876742718353764 39 0.5259232343322687 40 0.5928589187018303 41 0.5966838149515195 42 0.6464074661974794 43 0.6655319474459256 44 0.6597946030713917 45 0.6903937730689055 46 0.6884813249440609 47 0.732467631815487 48 0.7783663868117577 49 0.7401174243148655 50 0.7879286274359808 51 0.7515921130639331 52 0.7917535236856701 53 0.8013157643098932 54 0.81661534930865 55 0.8644265524297654 56 0.8472145193061639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 52289.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.76168983916311 #Duplication Level Percentage of deduplicated Percentage of total 1 78.63278032709731 38.342672455009655 2 11.181707651880615 10.904779207863987 3 4.2318704161273875 6.190594580122014 4 1.945326901204063 3.7942970796917135 5 1.1609208926540378 2.830423224770028 6 0.737341648037024 2.1572414848247243 7 0.3922030042750127 1.3387136873912295 8 0.2745421029925089 1.0709709499129836 9 0.184335412009256 0.8089655568092715 >10 1.0824802917990353 9.18548834362868 >50 0.08628466094050281 2.979594178507908 >100 0.0705965407695023 7.4069115875231875 >500 0.007844060085500256 2.914570942263191 >1k 0.011766090128250382 10.074776721681426 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 2498 4.777295415861844 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1551 2.9662070416339956 No Hit AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1219 2.331274264185584 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 947 1.811088374227849 No Hit AAAGTTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 577 1.1034825680353422 No Hit AAAGTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 461 0.8816385855533669 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 415 0.7936659718105147 No Hit AAAGTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 360 0.6884813249440609 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGT 341 0.6521448105720132 No Hit AAAGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 322 0.6158082961999656 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 253 0.4838493755856872 No Hit AAAGTTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 214 0.4092638987167473 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC 184 0.3518904549714089 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGA 175 0.3346784218478074 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 167 0.31937883684905044 No Hit AAAGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 128 0.24479335998011054 No Hit AAAGTTGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 127 0.24288091185526592 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC 127 0.24288091185526592 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 126 0.2409684637304213 Illumina Single End Adapter 2 (95% over 23bp) AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 124 0.2371435674807321 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAGCAGG 121 0.23140622310619824 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 118 0.22566887873166439 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.2103692937329075 No Hit AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.18933236435961673 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.1835950199850829 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.1835950199850829 No Hit AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 95 0.18168257186023828 No Hit AAAGTTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT 92 0.17594522748570446 No Hit AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.15682074623725833 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.1529958499875691 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA 80 0.1529958499875691 No Hit AAAGTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGAAGCTGAAAAACATCGA 76 0.14534605748819063 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 72 0.13769626498881218 Illumina Single End Adapter 2 (96% over 30bp) AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAA 71 0.13578381686396757 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTAAAGTTGGGAAGCAGT 70 0.13387136873912295 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.11283443936583219 No Hit AAAGTTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG 59 0.11283443936583219 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.11092199124098757 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 56 0.10709709499129837 No Hit AAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55 0.10518464686645375 No Hit AAAGTTGGGCAGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAA 54 0.10327219874160913 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCGGTGGGAAGGGCGGGTAGGCCG 53 0.10135975061676451 No Hit AAAGTTGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 53 0.10135975061676451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.061198339995027634 0.0 0.0 0.0 0.0 11 0.09944730249191991 0.0 0.0 0.0 0.0 12 0.10327219874160913 0.0 0.0 0.0 0.0 13 0.11092199124098759 0.0 0.0 0.0 0.0 14 0.14152116123850142 0.0 0.0 0.0 0.0 15 0.143433609363346 0.0 0.0 0.0 0.0 16 0.14725850561303525 0.0 0.0 0.0 0.0 17 0.15108340186272448 0.0 0.0 0.0 0.0 18 0.1549082981124137 0.0 0.0 0.0 0.0 19 0.15873319436210293 0.0 0.0 0.0 0.0 20 0.19889460498383982 0.0 0.0 0.0 0.0 21 0.2103692937329075 0.0 0.0 0.0 0.0 22 0.24479335998011054 0.0 0.0 0.0 0.0 23 0.27156763372793513 0.0 0.0 0.0 0.0 24 0.3059916999751382 0.0 0.0 0.0 0.0 25 0.3193788368490505 0.0 0.0 0.0 0.0 26 0.3346784218478074 0.0 0.0 0.0 0.0 27 0.3385033180974966 0.0 0.0 0.0 0.0 28 0.47046223871177495 0.0 0.0 0.0 0.0 29 0.48576182371053184 0.0 0.0 0.0 0.0 30 0.5526975080800933 0.0 0.0 0.0 0.0 31 0.5679970930788503 0.0 0.0 0.0 0.0 32 0.5718219893285394 0.0 0.0 0.0 0.0 33 0.5852091262024518 0.0 0.0 0.0 0.0 34 0.5928589187018302 0.0 0.0 0.0 0.0 35 0.79175352368567 0.0 0.0 0.0 0.0 36 0.8089655568092715 0.0 0.0 0.0 0.0 37 0.8147029011838054 0.0 0.0 0.0 0.0 38 0.8300024861825623 0.0 0.0 0.0 0.0 39 0.84147717493163 0.0 0.0 0.0 0.0 40 0.851039415555853 0.0 0.0 0.0 0.0 41 0.8739887930539884 0.0 0.0 0.0 0.0 42 0.8969381705521238 0.0 0.0 0.0 0.0 43 0.9217999961751038 0.0 0.0 0.0 0.0 44 0.935187133049016 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATATGG 20 4.033138E-4 50.282127 50 TTTTATG 15 0.008201431 50.282127 50 CCTTCTC 100 0.0 50.282127 50 GCATCGA 20 4.033138E-4 50.282127 50 CATCGAA 80 0.0 50.282127 50 GAGCGTG 90 0.0 50.233593 42 ACCGGGG 15 0.00823284 50.233593 45 CACCGGG 15 0.00823284 50.233593 44 AGAGGGG 20 4.0524383E-4 50.233593 43 GAAAAGC 15 0.00823284 50.233593 45 CGTAGCG 60 0.0 50.233593 41 CATCACC 15 0.00823284 50.233593 41 AAAAGCA 15 0.00823284 50.233593 46 CGGGAAG 20 4.0524383E-4 50.233593 46 ATCACCG 15 0.00823284 50.233593 42 AGCATCG 15 0.00823284 50.233593 49 TCACCGG 15 0.00823284 50.233593 43 CCGGGGC 15 0.00823284 50.233593 46 GGTATGC 20 4.0524383E-4 50.233593 48 GGAGCGT 90 0.0 50.233593 41 >>END_MODULE