##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212627_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93675
Sequences flagged as poor quality	0
Sequence length	58
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.673883106485185	39.0	38.0	39.0	34.0	40.0
2	36.71113957832933	38.0	35.0	39.0	32.0	40.0
3	36.57346143581532	39.0	35.0	39.0	31.0	40.0
4	36.99471577261809	39.0	36.0	39.0	33.0	40.0
5	37.46251401120897	39.0	38.0	39.0	34.0	40.0
6	36.77284227381906	38.0	35.0	39.0	33.0	40.0
7	36.64818788364025	38.0	35.0	39.0	31.0	40.0
8	37.08872164398185	39.0	37.0	39.0	33.0	40.0
9	36.94631438484121	39.0	37.0	39.0	33.0	40.0
10	35.6230904723779	38.0	35.0	39.0	29.0	40.0
11	35.98454230050707	38.0	35.0	39.0	30.0	40.0
12	35.65847878302642	38.0	35.0	39.0	29.0	40.0
13	35.61981318388044	38.0	35.0	39.0	29.0	40.0
14	35.53797704830531	38.0	35.0	39.0	29.0	40.0
15	35.576696023485454	38.0	35.0	39.0	29.0	40.0
16	35.15906058179877	38.0	34.0	39.0	28.0	40.0
17	35.14203362690152	38.0	34.0	39.0	28.0	40.0
18	34.95474779823859	38.0	33.0	39.0	27.0	40.0
19	34.47459834534294	38.0	33.0	39.0	26.0	40.0
20	34.67987189751801	38.0	33.0	39.0	27.0	40.0
21	34.47917800907393	38.0	33.0	39.0	26.0	40.0
22	34.2554470242861	38.0	33.0	39.0	26.0	40.0
23	33.90759540966106	37.0	33.0	39.0	25.0	40.0
24	33.92902054977315	37.0	33.0	39.0	25.0	40.0
25	33.84889244729116	37.0	33.0	39.0	25.0	40.0
26	33.31593274619696	37.0	33.0	39.0	22.0	40.0
27	33.27877235121431	37.0	33.0	39.0	22.0	40.0
28	32.66524686415799	36.0	31.0	39.0	20.0	40.0
29	32.91448091806779	36.0	32.0	39.0	21.0	40.0
30	32.361889511609284	36.0	31.0	39.0	18.0	40.0
31	31.48470776621297	36.0	30.0	38.0	11.0	40.0
32	31.138852415265546	36.0	29.0	38.0	9.0	40.0
33	30.97346143581532	35.0	29.0	38.0	8.0	40.0
34	30.625695222844943	35.0	29.0	38.0	5.0	40.0
35	30.218233253269283	35.0	28.0	38.0	2.0	40.0
36	30.622514011208967	36.0	29.0	39.0	2.0	40.0
37	30.47389378169202	36.0	29.0	39.0	2.0	40.0
38	30.404718441419803	36.0	29.0	39.0	2.0	40.0
39	30.3671096877502	36.0	29.0	39.0	2.0	40.0
40	30.233210568454762	35.0	28.0	39.0	2.0	40.0
41	30.48210301574593	36.0	30.0	39.0	2.0	40.0
42	30.39720309580998	36.0	29.0	39.0	2.0	40.0
43	30.21741126234321	36.0	29.0	39.0	2.0	40.0
44	30.15520683213237	36.0	29.0	39.0	2.0	40.0
45	29.72601014144649	35.0	28.0	39.0	2.0	40.0
46	29.511790765946092	35.0	28.0	39.0	2.0	40.0
47	29.486394448892447	35.0	28.0	39.0	2.0	40.0
48	29.347403255938083	35.0	27.0	39.0	2.0	40.0
49	29.225545769949292	35.0	27.0	39.0	2.0	40.0
50	28.97191353082466	35.0	26.0	39.0	2.0	40.0
51	28.682135041366426	35.0	26.0	39.0	2.0	40.0
52	28.533792367227115	35.0	26.0	39.0	2.0	40.0
53	28.225695222844944	35.0	24.0	39.0	2.0	40.0
54	28.050493728315985	35.0	23.0	39.0	2.0	40.0
55	27.67464104617027	35.0	22.0	39.0	2.0	40.0
56	27.10022951694689	35.0	2.0	39.0	2.0	40.0
57	26.82293034427542	35.0	2.0	39.0	2.0	39.0
58	26.391619962636778	35.0	2.0	38.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-2.863573070867922
1	2	-6.004891740337513
1	3	-4.209290880379186
1	4	-2.4869885041675985
1	5	-2.5600310150276826
1	6	-5.983170906939996
1	7	-11.963413112561724
1	8	-2.190335949818156
1	9	-4.050384083464991
1	10	-0.08867722623005392
1	11	-5.48452361139973
1	12	-4.983570779080495
1	13	-3.9537447255769393
1	14	1.2567692478270942
1	15	0.9543198426704222
1	16	-2.134424426594393
1	17	-24.576642159137926
1	18	-14.288141615959603
1	19	-12.088263488753157
1	20	-6.395870789128537
1	21	0.8267691948360749
1	22	-5.528906587695623
1	23	-12.832616351305415
1	24	-12.89716399084286
1	25	-6.700320937507339
1	26	-13.708202998279528
1	27	-14.682088486010631
1	28	-9.398612534506015
1	29	-0.13685820734735188
1	30	-15.699176759965418
1	31	-0.6264764291376714
1	32	-13.170125954352748
1	33	-5.172981407331175
1	34	3.6972286462855557
1	35	-24.729708619734808
1	36	-24.832530374665836
1	37	-24.836532816923935
1	38	-24.604381668992048
1	39	-24.684473821205525
1	40	-24.24678225509837
1	41	-24.07820455618402
1	42	-24.509460500191942
1	43	-24.041168432728803
1	44	-24.135498933729732
1	45	-23.10811607104498
1	46	-23.59903387623744
1	47	-23.572599997190476
1	48	-23.414380443307778
1	49	-23.480767681851585
1	50	-22.82558064781567
1	51	-22.861561436291737
1	52	-22.699293344699942
1	53	-22.23151312747697
1	54	-22.427835119929192
1	55	-21.886774243337324
1	56	-21.149136148797457
1	57	-20.904278845002757
1	58	-20.35683593768423
2	1	1.136426929132078
2	2	1.4951082596624872
2	3	3.7907091196208142
2	4	0.013011495832401465
2	5	1.4399689849723174
2	6	2.0168290930600037
2	7	-3.4634131125617245
2	8	0.8096640501818442
2	9	0.9496159165350093
2	10	2.911322773769946
2	11	-2.48452361139973
2	12	-1.9835707790804946
2	13	-1.9537447255769393
2	14	-2.743230752172906
2	15	1.4543198426704222
2	16	-0.6344244265943928
2	17	3.4233578408620744
2	18	1.2118583840403971
2	19	1.4117365112468434
2	20	0.10412921087146287
2	21	-2.173230805163925
2	22	4.471093412304377
2	23	-0.8326163513054148
2	24	-2.3971639908428592
2	25	2.299679062492661
2	26	-2.7082029982795284
2	27	-0.6820884860106311
2	28	2.1013874654939855
2	29	2.363141792652648
2	30	-4.199176759965418
2	31	5.373523570862329
2	32	4.8298740456472515
2	33	3.827018592668825
2	34	5.697228646285556
2	35	5.270291380265192
2	36	1.6674696253341637
2	37	6.663467183076065
2	38	4.895618331007952
2	39	5.3155261787944745
2	40	0.2532177449016295
2	41	3.4217954438159808
2	42	4.990539499808058
2	43	-4.041168432728803
2	44	4.364501066270268
2	45	4.391883928955021
2	46	10.900966123762561
2	47	12.427400002809524
2	48	12.585619556692222
2	49	10.019232318148415
2	50	9.17441935218433
2	51	10.138438563708263
2	52	8.800706655300058
2	53	13.768486872523031
2	54	10.072164880070808
2	55	11.113225756662676
2	56	14.350863851202543
2	57	13.595721154997243
2	58	13.143164062315769
3	1	1.136426929132078
3	2	2.995108259662487
3	3	1.7907091196208142
3	4	2.5130114958324015
3	5	1.4399689849723174
3	6	3.0168290930600037
3	7	2.0365868874382755
3	8	1.8096640501818442
3	9	-0.05038408346499068
3	10	3.911322773769946
3	11	4.51547638860027
3	12	5.016429220919505
3	13	5.046255274423061
3	14	5.256769247827094
3	15	4.954319842670422
3	16	4.865575573405607
3	17	5.423357840862074
3	18	2.711858384040397
3	19	4.911736511246843
3	20	5.604129210871463
3	21	2.826769194836075
3	22	3.471093412304377
3	23	-0.8326163513054148
3	24	-0.8971639908428592
3	25	-0.7003209375073389
3	26	5.291797001720472
3	27	3.317911513989369
3	28	5.6013874654939855
3	29	7.863141792652648
3	30	6.300823240034582
3	31	9.373523570862329
3	32	9.829874045647252
3	33	0.8270185926688249
3	34	5.697228646285556
3	35	8.270291380265192
3	36	4.167469625334164
3	37	4.163467183076065
3	38	7.395618331007952
3	39	5.3155261787944745
3	40	7.7532177449016295
3	41	5.921795443815981
3	42	8.490539499808058
3	43	6.958831567271197
3	44	8.864501066270268
3	45	-2.1081160710449787
3	46	11.400966123762561
3	47	12.427400002809524
3	48	10.585619556692222
3	49	11.519232318148415
3	50	9.17441935218433
3	51	11.138438563708263
3	52	11.300706655300058
3	53	8.768486872523031
3	54	14.572164880070808
3	55	15.113225756662676
3	56	13.850863851202543
3	57	14.095721154997243
3	58	4.643164062315769
4	1	-1.363573070867922
4	2	-3.504891740337513
4	3	-4.209290880379186
4	4	-1.4869885041675985
4	5	-1.0600310150276826
4	6	-3.9831709069399963
4	7	-13.463413112561724
4	8	-6.190335949818156
4	9	-6.050384083464991
4	10	-4.588677226230054
4	11	-0.4845236113997302
4	12	0.5164292209195054
4	13	0.046255274423060655
4	14	1.7567692478270942
4	15	-0.5456801573295778
4	16	-2.634424426594393
4	17	-0.5766421591379256
4	18	-1.2881416159596029
4	19	-1.0882634887531566
4	20	-2.895870789128537
4	21	1.826769194836075
4	22	4.471093412304377
4	23	0.16738364869458522
4	24	1.6028360091571408
4	25	-4.200320937507339
4	26	-8.708202998279528
4	27	-12.682088486010631
4	28	-7.8986125345060145
4	29	-1.6368582073473519
4	30	-9.699176759965418
4	31	-3.6264764291376714
4	32	2.3298740456472515
4	33	-8.172981407331175
4	34	-5.802771353714444
4	35	-2.229708619734808
4	36	-16.832530374665836
4	37	-9.336532816923935
4	38	-11.604381668992048
4	39	-6.6844738212055255
4	40	-4.2467822550983705
4	41	-9.07820455618402
4	42	-6.009460500191942
4	43	-6.541168432728803
4	44	-3.635498933729732
4	45	-13.108116071044979
4	46	6.4009661237625615
4	47	5.927400002809524
4	48	4.585619556692222
4	49	6.019232318148415
4	50	3.67441935218433
4	51	9.138438563708263
4	52	6.800706655300058
4	53	-6.731513127476969
4	54	-5.927835119929192
4	55	-5.3867742433373245
4	56	-10.149136148797457
4	57	-6.904278845002757
4	58	-9.856835937684231
5	1	-1.863573070867922
5	2	-1.0048917403375128
5	3	-4.209290880379186
5	4	-1.8203218375009342
5	5	-1.5600310150276826
5	6	-3.649837573606664
5	7	-0.6300797792283888
5	8	-0.8570026164848201
5	9	-0.717050750131655
5	10	-1.4220105595633896
5	11	-1.4845236113997302
5	12	-2.9835707790804946
5	13	-4.953744725576939
5	14	0.2567692478270942
5	15	-1.0456801573295778
5	16	-6.134424426594393
5	17	-3.9099754924712578
5	18	-4.954808282626271
5	19	-6.421596822086489
5	20	0.9374625442047986
5	21	-10.506564138497257
5	22	-7.862239921028955
5	23	-1.165949684638747
5	24	-1.8971639908428592
5	25	-5.700320937507339
5	26	-3.3748696649461962
5	27	-2.0154218193439633
5	28	-1.3986125345060145
5	29	-2.136858207347352
5	30	-4.03251009329875
5	31	2.3735235708623286
5	32	-0.8367926210194163
5	33	5.827018592668825
5	34	-6.302771353714444
5	35	-7.396375286401476
5	36	-5.499197041332504
5	37	-6.836532816923935
5	38	-5.93771500232538
5	39	-5.351140487872193
5	40	-3.9134489217650383
5	41	-3.4115378895173514
5	42	-3.509460500191942
5	43	-3.7078350993954707
5	44	-3.8021656003963997
5	45	-3.441449404378311
5	46	-13.599033876237439
5	47	-9.239266663857144
5	48	-9.414380443307778
5	49	-9.480767681851585
5	50	-7.492247314482338
5	51	-8.528228102958405
5	52	-7.0326266780332745
5	53	-5.898179794143637
5	54	-3.761168453262524
5	55	-4.553440910003992
5	56	0.5175305178692113
5	57	-4.237612178336089
5	58	-1.0235026043508988
6	1	-0.8635730708679219
6	2	-2.504891740337513
6	3	-1.2092908803791858
6	4	-1.9869885041675985
6	5	-1.5600310150276826
6	6	-1.9831709069399963
6	7	-2.9634131125617245
6	8	-0.6903359498181558
6	9	-0.5503840834649907
6	10	-4.338677226230054
6	11	1.5154763886002698
6	12	0.7664292209195054
6	13	2.0462552744230607
6	14	-3.243230752172906
6	15	1.9543198426704222
6	16	-2.384424426594393
6	17	1.6733578408620744
6	18	-0.28814161595960286
6	19	0.9117365112468434
6	20	1.1041292108714629
6	21	-1.923230805163925
6	22	0.7210934123043771
6	23	-3.332616351305415
6	24	-0.8971639908428592
6	25	-2.450320937507339
6	26	-1.9582029982795284
6	27	-1.682088486010631
6	28	1.8513874654939855
6	29	4.113141792652648
6	30	-5.199176759965418
6	31	-0.8764764291376714
6	32	-1.4201259543527485
6	33	-5.172981407331175
6	34	-3.0527713537144443
6	35	-0.7297086197348079
6	36	-4.582530374665836
6	37	0.4134671830760652
6	38	-0.6043816689920476
6	39	-3.9344738212055255
6	40	-5.4967822550983705
6	41	1.9217954438159808
6	42	2.240539499808058
6	43	-0.7911684327288029
6	44	1.1145010662702681
6	45	-2.1081160710449787
6	46	6.9009661237625615
6	47	7.427400002809524
6	48	7.835619556692222
6	49	7.519232318148415
6	50	6.17441935218433
6	51	8.638438563708263
6	52	5.800706655300058
6	53	8.268486872523031
6	54	8.072164880070808
6	55	6.1132257566626755
6	56	2.8508638512025435
6	57	2.3457211549972428
6	58	-0.35683593768423094
7	1	-4.196906404201258
7	2	-5.338225073670845
7	3	-7.542624213712518
7	4	-7.153655170834266
7	5	-6.560031015027683
7	6	-7.983170906939996
7	7	-15.630079779228392
7	8	-9.190335949818156
7	9	-8.383717416798323
7	10	-15.422010559563386
7	11	-5.48452361139973
7	12	-3.3169041124138268
7	13	-7.2870780589102715
7	14	-3.743230752172906
7	15	-5.712346823996246
7	16	1.198908906738943
7	17	-3.2433088258045935
7	18	-0.621474949292935
7	19	-5.421596822086489
7	20	-2.062537455795205
7	21	1.1601025281694106
7	22	-3.862239921028955
7	23	-12.165949684638747
7	24	-11.230497324176191
7	25	-11.033654270840671
7	26	-10.04153633161286
7	27	-7.348755152677299
7	28	-11.731945867839347
7	29	-11.470191540680684
7	30	-9.03251009329875
7	31	-6.293143095804339
7	32	-11.836792621019416
7	33	-7.506314740664507
7	34	-4.969438020381112
7	35	-13.729708619734808
7	36	-13.832530374665836
7	37	-15.169866150257269
7	38	-13.604381668992048
7	39	-13.684473821205525
7	40	-13.24678225509837
7	41	-13.07820455618402
7	42	-13.509460500191942
7	43	-15.041168432728803
7	44	-13.135498933729732
7	45	-12.108116071044979
7	46	-12.599033876237439
7	47	-12.572599997190476
7	48	-12.414380443307778
7	49	-12.480767681851585
7	50	-11.82558064781567
7	51	-11.861561436291737
7	52	-11.699293344699942
7	53	-11.231513127476969
7	54	-11.427835119929192
7	55	-10.886774243337324
7	56	-10.149136148797457
7	57	-9.904278845002757
7	58	-9.356835937684231
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119	3	-2.2092908803791858
119	4	-1.4869885041675985
119	5	-1.0600310150276826
119	6	-1.9831709069399963
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119	14	-2.243230752172906
119	15	1.4543198426704222
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119	18	-1.7881416159596029
119	19	-6.088263488753157
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119	21	1.826769194836075
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119	28	-17.898612534506015
119	29	-17.636858207347352
119	30	-12.699176759965418
119	31	-19.12647642913767
119	32	-18.67012595435275
119	33	-17.672981407331175
119	34	-14.302771353714444
119	35	-10.229708619734808
119	36	-9.832530374665836
119	37	-24.836532816923935
119	38	-24.604381668992048
119	39	-24.684473821205525
119	40	-24.24678225509837
119	41	-24.07820455618402
119	42	-24.509460500191942
119	43	-24.041168432728803
119	44	-24.135498933729732
119	45	-23.10811607104498
119	46	-23.59903387623744
119	47	-23.572599997190476
119	48	-23.414380443307778
119	49	-23.480767681851585
119	50	-22.82558064781567
119	51	-22.861561436291737
119	52	-22.699293344699942
119	53	-22.23151312747697
119	54	-22.427835119929192
119	55	-21.886774243337324
119	56	-21.149136148797457
119	57	-20.904278845002757
119	58	-20.35683593768423
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	272.0
3	16.0
4	121.0
5	87.0
6	201.0
7	390.0
8	217.0
9	340.0
10	402.0
11	419.0
12	542.0
13	677.0
14	624.0
15	781.0
16	915.0
17	951.0
18	1074.0
19	1132.0
20	1267.0
21	1007.0
22	1131.0
23	1176.0
24	1299.0
25	1240.0
26	1376.0
27	1743.0
28	2020.0
29	2482.0
30	2906.0
31	3540.0
32	4153.0
33	4913.0
34	5666.0
35	6637.0
36	7887.0
37	11217.0
38	12827.0
39	9989.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	39.31144915932746	37.086736055511075	6.199092607419269	17.402722177742195
11	26.689084601014144	31.506805444355486	19.196156925540432	22.607953029089938
12	41.69949292767547	15.714971977582065	27.009340805978116	15.576194288764345
13	17.465705898051777	18.184147317854283	33.165732586068856	31.184414198025085
14	17.835068054443553	32.10888710968775	33.421937550040035	16.634107285828666
15	31.64771817453963	15.469442220443023	32.737656792100346	20.145182812917
16	20.230584467574058	16.3629570322925	38.283426741393114	25.12303175874033
17	32.18788364024553	15.389378169202029	22.95809981318388	29.464638377368562
18	33.44969308780358	18.163864424873232	24.535895382973045	23.85054710435015
19	25.484921270349613	18.54923939151321	36.38323992527355	19.582599412863626
20	27.307179076594608	29.32372564718441	22.08486789431545	21.284227381905524
21	27.98244958525936	19.767702539685928	32.118113010152335	20.131734864902374
22	29.182812917000266	20.56685348278623	20.27969041900187	29.97064318121164
23	27.68383294901358	32.898838500298915	20.563241950636264	18.854086600051243
24	25.770224395243073	32.50314921963405	22.504643764545122	19.221982620577748
25	23.822791566586602	20.644782492660795	22.57592740859354	32.95649853215906
26	32.56670617252314	19.843684935455972	22.506593207128137	25.083015684892747
27	21.887009992313605	19.221325476129472	23.717866598343154	35.17379793321377
28	22.713149324648978	29.974907906678766	25.020554161550372	22.291388607121885
29	32.33328350878691	18.65858085456215	25.84612756507442	23.16200807157652
30	22.809696710807348	28.960914139256726	27.723195215719777	20.506193934216146
31	34.62191514581976	20.779983554563607	28.289354249650266	16.308747049966364
32	29.48706997800508	17.72833073522817	37.592090371351084	15.192508915415662
33	30.016868807652884	17.493380594465325	32.26746668944311	20.222283908438673
34	23.994361805503647	22.927589778636793	33.69889051437846	19.379157901481094
35	23.09203711811377	32.92043525152968	31.674266127051588	12.313261503304965
36	24.787192002477866	28.615065844983928	29.88283544628267	16.71490670625554
37	22.635463507898702	25.32924601860654	36.30838575990942	15.726904713585338
38	23.517220750363215	26.75839671822921	34.05691821211862	15.667464319288948
39	23.154487871051817	24.72040931862122	32.81812452600434	19.306978284322625
40	23.334757530442214	22.771843623157444	37.176885280922875	16.716513565477463
41	20.305940414258703	25.061690149872344	38.550201360922095	16.082168074946857
42	22.303788000213707	25.329913981941548	34.32494523695036	18.041352780894375
43	20.355391240236358	22.166540224603846	35.36922863218182	22.108839902977977
44	21.587060796735287	21.013385751065623	38.390291324366764	19.009262127832322
45	21.15624966617884	22.646427312445923	39.65902171706921	16.538301304306025
46	21.6802341730231	23.948251180479886	36.06606414118753	18.30545050530949
47	23.173195105007213	23.87538075134933	35.70993427029338	17.241489873350076
48	23.422855371856667	23.428198907781255	35.41161257227132	17.737333148090755
49	21.041884928695133	24.451048726775138	35.01314917363324	19.493917170896495
50	17.769058895220173	23.567709725355197	38.760303189044144	19.902928190380482
51	15.671227020094058	25.90637024369389	40.93202223172296	17.490380504489096
52	17.09522282782943	23.95639628085925	40.063054397777066	18.88532649353425
53	15.460842793919568	24.986637590062642	40.831248797383104	18.721270818634682
54	16.556723295521365	25.524681128585634	37.90533822287321	20.01325735301979
55	17.5310648672926	24.46585537994311	35.46634728489852	22.536732467865775
56	16.772551284519455	25.937453207555244	36.42751716614259	20.86247834178271
57	19.353389730810775	25.525384212271263	34.51974802947499	20.601478027442973
58	19.21295553487576	27.060937650418765	35.950753564590485	17.775353250114986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	153.5
9	260.0
10	213.0
11	188.5
12	164.0
13	188.5
14	213.0
15	433.0
16	760.5
17	868.0
18	945.5
19	1023.0
20	1185.0
21	1347.0
22	1554.5
23	2023.5
24	2285.0
25	2545.5
26	2806.0
27	3073.5
28	3387.5
29	3434.0
30	3047.5
31	2661.0
32	3661.0
33	4661.0
34	3352.0
35	1822.5
36	1602.0
37	1544.5
38	1487.0
39	1580.0
40	1673.0
41	1777.0
42	2274.5
43	2668.0
44	2763.0
45	2858.0
46	2615.5
47	2392.0
48	2411.0
49	2649.0
50	2887.0
51	3853.0
52	4819.0
53	4696.5
54	4441.0
55	4308.0
56	4554.5
57	4801.0
58	5524.5
59	6639.5
60	7031.0
61	6090.0
62	5149.0
63	4379.5
64	3610.0
65	2912.0
66	1816.0
67	1418.0
68	1094.0
69	770.0
70	648.0
71	526.0
72	413.5
73	215.5
74	130.0
75	97.0
76	64.0
77	53.5
78	34.0
79	25.0
80	19.0
81	13.0
82	8.0
83	3.0
84	3.5
85	3.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0021350413664264745
22	0.0
23	0.0032025620496397116
24	0.0010675206832132373
25	0.0
26	0.01921537229783827
27	0.0032025620496397116
28	0.021350413664264743
29	0.013877768881772086
30	0.03736322391246331
31	0.03416066186282359
32	0.018147851614625034
33	0.01174272751534561
34	0.029890579129970646
35	0.029890579129970646
36	0.049105951427808915
37	0.055511075527088345
38	0.0715238857752869
39	0.05978115825994129
40	0.05871363757672805
41	0.06618628235922071
42	0.09607686148919135
43	0.09394182012276488
44	0.07259140645850014
45	0.06618628235922071
46	0.07365892714171338
47	0.1174272751534561
48	0.11102215105417669
49	0.14198025086736055
50	0.14518281291700028
51	0.12276487856952228
52	0.11208967173738991
53	0.1377101681345076
54	0.15158793701627968
55	0.1718708299973312
56	0.188951160928743
57	0.1836135575126768
58	0.1985588470776621
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	93675.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.3549506271684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78136010869815	36.055511075527086
2	10.692490154986988	9.912997064318121
3	4.20284181194298	5.844675740592474
4	2.1509338369067086	3.9882572724846543
5	1.2620040070930152	2.9250066720042702
6	0.7668746977408286	2.132906325060048
7	0.543490776777284	1.763544168668268
8	0.4444649149068466	1.6482519348812383
9	0.271745388388642	1.133706965572458
>10	1.6212606222508807	13.841473178542834
>50	0.1519931833360201	4.691753402722178
>100	0.09672293485019459	9.22658126501201
>500	0.009211708080970915	2.8684280757939686
>1k	0.004605854040485457	3.9669068588203897
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	2496	2.6645316253002402	No Hit
TACTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	1220	1.3023752335201495	No Hit
TACTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	778	0.8305310915398986	No Hit
TACTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	708	0.755804643714972	No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	629	0.6714705097411263	No Hit
TACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	572	0.6106218307979717	No Hit
TACTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	451	0.48145182812917003	No Hit
TACTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	445	0.47504670402989063	No Hit
TACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	444	0.4739791833466773	No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	434	0.46330397651454497	No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	400	0.4270082732852949	No Hit
TACTTCGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	368	0.3928476114224713	No Hit
TACTTCGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG	325	0.3469442220443021	No Hit
TACTTCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	290	0.3095809981318388	No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	262	0.27969041900186814	No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	261	0.2786228983186549	No Hit
TACTTCGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	250	0.26688017080330934	No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	235	0.2508673605551107	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAGCAGTGG	226	0.24125967440619162	No Hit
TACTTCGGGCTTACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT	218	0.23271950894048574	No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	217	0.23165198825727248	No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	210	0.22417934347477983	No Hit
TACTTCGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	205	0.21884174005871362	No Hit
TACTTCGGGGTGCTTACTTCGGGCAGTGGTAGCTTACTTCGGGCTTTTCCTGTGGGAG	197	0.21030157459300775	No Hit
TACTTCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	186	0.1985588470776621	No Hit
TACTTCGGGGCTTACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA	177	0.188951160928743	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	173	0.18468107819589002	Illumina Single End Adapter 2 (96% over 25bp)
TACTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153	0.1633306645316253	No Hit
TACTTCGGGGCTTTTCCTGTAGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	152	0.16226314384841206	No Hit
TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152	0.16226314384841206	No Hit
TACTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	149	0.15906058179877236	No Hit
TACTTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC	148	0.1579930611155591	No Hit
TACTTCGGGAGGCCAATCTTCTCTGAAGAATTCCTTCTTAAGGCTCCTTCCCAGTGAT	146	0.15585801974913263	No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	140	0.14945289564985323	No Hit
TACTTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC	133	0.14198025086736055	No Hit
TACTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCT	128	0.13664264745129437	No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	128	0.13664264745129437	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	125	0.13344008540165467	No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	124	0.13237256471844142	No Hit
TACTTCGGGAGCCCATCCCCAGCAATATCCCCAACCGGTCTACCTTCGCCTGCCCCTA	122	0.13023752335201497	No Hit
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.12383239925273552	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA	113	0.12062983720309581	No Hit
TACTTCGGGAACCATTGTACTTACCATCACAGTAATATACCAATGTCTCTTGGACCTT	110	0.1174272751534561	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	108	0.11529223378702962	Illumina Single End Adapter 2 (96% over 32bp)
TACTTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC	107	0.11422471310381639	No Hit
TACTTCGGGAGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	107	0.11422471310381639	No Hit
TACTTCGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG	104	0.11102215105417669	No Hit
TACTTCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC	104	0.11102215105417669	No Hit
TACTTCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	100	0.10675206832132372	No Hit
TACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA	98	0.10461702695489725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.016012810248198558	0.0	0.0	0.0	0.0
11	0.028823058446757407	0.0	0.0	0.0	0.0
12	0.029890579129970642	0.0	0.0	0.0	0.0
13	0.033093141179610354	0.0	0.0	0.0	0.0
14	0.05551107552708834	0.0	0.0	0.0	0.0
15	0.058713637576728046	0.0	0.0	0.0	0.0
16	0.06084867894315452	0.0	0.0	0.0	0.0
17	0.06511876167600747	0.0	0.0	0.0	0.0
18	0.06511876167600747	0.0	0.0	0.0	0.0
19	0.06511876167600747	0.0	0.0	0.0	0.0
20	0.08219909260741927	0.0	0.0	0.0	0.0
21	0.08753669602348546	0.0	0.0	0.0	0.0
22	0.09073925807312516	0.0	0.0	0.0	0.0
23	0.09821190285561783	0.0	0.0	0.0	0.0
24	0.11529223378702963	0.0	0.0	0.0	0.0
25	0.12062983720309581	0.0	0.0	0.0	0.0
26	0.12383239925273552	0.0	0.0	0.0	0.0
27	0.12810248198558846	0.0	0.0	0.0	0.0
28	0.24552975713904457	0.0	0.0	0.0	0.0
29	0.24873231918868427	0.0	0.0	0.0	0.0
30	0.2957032292500667	0.0	0.0	0.0	0.0
31	0.29890579129970646	0.0	0.0	0.0	0.0
32	0.30851347744862556	0.0	0.0	0.0	0.0
33	0.31064851881505207	0.0	0.0	0.0	0.0
34	0.3181211635975447	0.0	0.0	0.0	0.0
35	0.440886042167067	0.0	0.0	0.0	0.0
36	0.4494262076327729	0.0	0.0	0.0	0.0
37	0.45689885241526557	0.0	0.0	0.0	0.0
38	0.46330397651454497	0.0	0.0	0.0	0.0
39	0.4643714971977582	0.0	0.0	0.0	0.0
40	0.4686415799306112	0.0	0.0	0.0	0.0
41	0.4739791833466773	0.0	0.0	0.0	0.0
42	0.4835868694955965	0.0	0.0	0.0	0.0
43	0.4899919935948759	0.0	0.0	0.0	0.0
44	0.4953295970109421	0.0	0.0	0.0	0.0
45	0.5060048038430744	0.0	0.0	0.0	0.0
46	0.5166800106752069	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCTAC	15	0.0071995556	52.0	26
ATCCTGC	45	9.094947E-11	52.0	20
CAATGTC	15	0.0071995556	52.0	41
ACTAATC	15	0.0071995556	52.0	10
AATCCTA	15	0.0071995556	52.0	13
AAACTGG	15	0.0071995556	52.0	27
CCAATGT	15	0.0071995556	52.0	40
GGGTCCT	15	0.0071995556	52.0	7
TATACTA	15	0.0071995556	52.0	44
ATCTTGA	15	0.0071995556	52.0	10
GGCATAA	15	0.0071995556	52.0	8
CAGCAAA	15	0.0071995556	52.0	46
TATATCC	15	0.0071995556	52.0	23
AGGATTT	25	1.6434518E-5	52.0	35
TGGTAGC	30	7.915896E-7	52.0	27
GAGTGAT	15	0.0071995556	52.0	29
TTGTACT	15	0.0071995556	52.0	15
TGACTCG	15	0.0071995556	52.0	14
CGCCCCG	25	1.6434518E-5	52.0	52
GACAGCT	15	0.0071995556	52.0	13
>>END_MODULE