FastQCFastQC Report
Tue 31 May 2016
SRR212626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100146
Sequences flagged as poor quality0
Sequence length58
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT27052.7010564575719447No Hit
TACTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT12441.242186407844547No Hit
TACTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8230.821800171749246No Hit
TACTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7830.78185848660955No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7000.6989794899446807No Hit
TACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6290.6280829988217204No Hit
TACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4820.4812973059333373No Hit
TACTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4590.4583308369780121No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA4570.4563337527210273No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4550.4543366684640425No Hit
TACTTCGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4330.43236874163720973No Hit
TACTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4250.42438040460927046No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3160.31553931260359874No Hit
TACTTCGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG2840.2835859644918419No Hit
TACTTCGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2790.2785932538493799No Hit
TACTTCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2770.27659616959239514No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2690.26860783256445586No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC2300.22966468955325225No Hit
TACTTCGGGGTGCTTACTTCGGGCAGTGGTAGCTTACTTCGGGCTTTTCCTGTGGGAG2250.22467197891079027No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2230.22267489465380544No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.22167635252531306No Hit
TACTTCGGGCTTACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT2100.20969384698340424No Hit
TACTTCGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2100.20969384698340424No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC2080.20769676272641946Illumina Single End Adapter 2 (96% over 25bp)
TACTTCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1970.196712799313003No Hit
TACTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.1837317516426018No Hit
TACTTCGGGAGGCCAATCTTCTCTGAAGAATTCCTTCTTAAGGCTCCTTCCCAGTGAT1760.17574341461466259No Hit
TACTTCGGGGCTTACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA1690.16875361971521577No Hit
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.15976674055878418No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1590.15876819843029177No Hit
TACTTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1540.15377548778782976No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAGCAGTGG1530.15277694565933736No Hit
TACTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.14079444011742856No Hit
TACTTCGGGAGCCCATCCCCAGCAATATCCCCAACCGGTCTACCTTCGCCTGCCCCTA1400.13979589798893616No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA1340.13380464521798174No Hit
TACTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCT1320.13180756096099694No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1280.12781339244702733No Hit
TACTTCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC1250.12481776606155014No Hit
TACTTCGGGAGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1240.12381922393305775No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1240.12381922393305775No Hit
TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.12082359754758054No Hit
TACTTCGGGGCTTTTCCTGTAGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1200.11982505541908813No Hit
TACTTCGGGAACCATTGTACTTACCATCACAGTAATATACCAATGTCTCTTGGACCTT1180.11782797116210333No Hit
TACTTCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1160.11583088690511852No Hit
TACTTCGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCT1150.11483234477662613No Hit
TACTTCGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG1120.11183671839114892No Hit
TACTTCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1120.11183671839114892No Hit
TACTTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1100.10983963413416412No Hit
TACTTCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1060.1058454656201945Illumina Single End Adapter 2 (96% over 32bp)
TACTTCGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA1030.1028498392347173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG150.007140314752.11128251
TATATAA150.007140314752.11128252
TAAAAGC150.007140314752.11128251
TTATATA150.007140314752.11128251
TCTATAG150.007140314752.11128250
ATTATAT150.007140314752.11128250
TCTCGTC150.007140314752.11128250
TCGTCCT150.007140314752.11128252
TGGACCT150.007140314752.11128251
TGGGATT150.007140314752.11128252
CTCGTCC150.007140314752.11128251
CGCCCCG401.8299033E-952.1112852
AGACCTT203.3963792E-452.1112850
GCCCCTA251.623562E-552.1112852
CGGAGAC550.052.11127552
CTCCTTC203.4047995E-452.0851744
CACGTGA150.00715448852.0851744
ACACCCG150.00715448852.0851749
GAGTTAC150.00715448852.0851744
TCGCCTG251.62839E-552.0851746
Produced by FastQC (version 0.11.5)