FastQCFastQC Report
Tue 31 May 2016
SRR212625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106003
Sequences flagged as poor quality0
Sequence length58
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT23942.2584266483024065No Hit
TACTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT12561.1848721262605775No Hit
TACTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7570.7141307321490901No Hit
TACTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7250.6839429072762092No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6740.6358310613850552No Hit
TACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6020.567908455421073No Hit
TACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5220.49243889323887063No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA4560.4301765044385536No Hit
TACTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4440.4188560701112233No Hit
TACTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4350.41036574436572554No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4080.38489476712923215No Hit
TACTTCGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG3930.37074422422006925No Hit
TACTTCGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3610.3405563993471883No Hit
TACTTCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2670.2518796637831005No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2670.2518796637831005No Hit
TACTTCGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2410.2273520560738847No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360.22263520843749707No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.21414488269199927No Hit
TACTTCGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2250.21225814363744425No Hit
TACTTCGGGCTTACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT2240.21131477411016672No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC2160.20376781789194645No Hit
TACTTCGGGGTGCTTACTTCGGGCAGTGGTAGCTTACTTCGGGCTTTTCCTGTGGGAG2130.20093770931011384No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAGCAGTGG2130.20093770931011384No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2060.19433412261917116No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1820.17169325396451043Illumina Single End Adapter 2 (96% over 25bp)
TACTTCGGGGCTTACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA1710.1613161891644576No Hit
TACTTCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1680.158486080582625No Hit
TACTTCGGGAGGCCAATCTTCTCTGAAGAATTCCTTCTTAAGGCTCCTTCCCAGTGAT1660.15659934152806998No Hit
TACTTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1600.15093912436440476No Hit
TACTTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1490.14056205956435194No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1430.13490184240068678No Hit
TACTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.12358140807335642No Hit
TACTTCGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG1300.1226380385460789No Hit
TACTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.11980792996424629No Hit
TACTTCGGGAGCCCATCCCCAGCAATATCCCCAACCGGTCTACCTTCGCCTGCCCCTA1270.11980792996424629No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1250.11792119090969125No Hit
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.11603445185513618No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1200.11320434327330359No Hit
TACTTCGGGGCTTTTCCTGTAGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1200.11320434327330359No Hit
TACTTCGGGAGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1120.10565738705508336No Hit
TACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA1100.10377064800052829No Hit
TACTTCGGGAACCATTGTACTTACCATCACAGTAATATACCAATGTCTCTTGGACCTT1090.10282727847325075No Hit
TACTTCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1080.10188390894597323No Hit
TACTTCGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTCT1080.10188390894597323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCT150.00712167952.14774338
AAACCGA150.00712167952.14774338
AAACCAC307.7717596E-752.14774352
AACCGAT150.00712167952.14774339
AGGATTA150.00712167952.14774344
ATCTATG150.00712167952.14774350
TAAGCCC150.00712167952.14774344
GGGTACG203.3855313E-452.1477437
GGATCTC150.00712167952.1477438
CGCTCCG800.052.14774352
GGATTGT203.3855313E-452.1477438
GGATTGA150.00712167952.1477438
TATGATT150.00712167952.14774340
GGTTTAC150.00712167952.1477439
CCCACAC150.00712167952.14774350
GAATGCT203.3855313E-452.14774344
ACCGATC150.00712167952.14774340
GGATATT150.00712167952.1477438
GATTGAA150.00712167952.1477439
GCTATGA150.00712167952.14774338
Produced by FastQC (version 0.11.5)