FastQCFastQC Report
Tue 31 May 2016
SRR212624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198124
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT83254.201913952877995No Hit
TACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC23371.1795643132583635No Hit
TACTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT20581.0387434132159656No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC14340.7237891421533988No Hit
TACTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA11980.6046718216874281No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11420.5764066947971977No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAGCAGT11100.560255193717066No Hit
TACTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA9810.4951444549877854No Hit
TACTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC9400.4744503442288668No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8560.4320526538935212No Hit
TACTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT7530.38006500979184754No Hit
TACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA7330.3699703216167653No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5900.29779330116492697No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.28668914417233654No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG4580.23116835920938403Illumina Single End Adapter 2 (95% over 23bp)
TACTTCGGGGTGCTTACTTCGGGCAGTGGTAGCTTACTTCGGGCTTTTCCTGTGGG4540.22914942157436757No Hit
TACTTCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC4510.2276352183481052No Hit
TACTTCGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4450.22460681189558057No Hit
TACTTCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4270.21552159253800648No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA4270.21552159253800648No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.2039127011366619No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3930.19836062264036664No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3930.19836062264036664No Hit
TACTTCGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3470.17514283983767742No Hit
TACTTCGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3380.1706002301588904No Hit
TACTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.16959076134138218No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAGCAGG3350.16908602693262803No Hit
TACTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.16757182370636572No Hit
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.16000080757505403No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.15394399467000464No Hit
TACTTCGGGGCTTTTCCTGTAGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3050.15394399467000464No Hit
TACTTCGGGGCCTGTGAGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC2820.14233510326866003No Hit
TACTTCGGGCTTACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC2800.1413256344511518No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2730.137792493589873No Hit
TACTTCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2690.13577355595485655No Hit
TACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC2680.13526882154610242No Hit
TACTTCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG2660.1342593527285942Illumina Single End Adapter 2 (96% over 30bp)
TACTTCGGGGCTTACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCT2650.1337546183198401No Hit
TACTTCGGGAGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2440.12315519573600371No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2240.11306050756092144No Hit
TACTTCGGGAGGCCAATCTTCTCTGAAGAATTCCTTCTTAAGGCTCCTTCCCAGTG2170.10952736669964265No Hit
TACTTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2140.1080131634733803No Hit
TACTTCGGGGTGCTTACTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGA2000.10094688175082273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTC150.00827971250.2140350
GGTTGCG204.0934357E-450.2012748
GGTAGGC204.0934357E-450.2012748
GTTGCGG204.0934357E-450.2012749
GACACAT150.00828803650.20126749
GGCTTGA252.0322455E-550.20126748
ACCTACC150.00828803650.20126749
ATCCCTA150.00828803650.20126748
GCTCGAA150.00829636850.18851546
TATCCCT150.00829636850.18851547
CAGGTTC150.00830470450.17576645
TTGGCCC204.103716E-450.17576645
GTTTGCG150.00831304850.16302542
ACCCTCC150.00831304850.16302544
TGATTGC150.00831304850.16302544
TTGTTGG204.108864E-450.16302542
ACCTTCG402.588422E-950.16302542
TAGTTCG150.00832975350.1375641
TAGGGCC150.00832975350.1375641
CTTAAAT150.00833811450.1248439
Produced by FastQC (version 0.11.5)