FastQCFastQC Report
Tue 31 May 2016
SRR212623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182780
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT71173.8937520516467887No Hit
TACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC24391.3343910712331766No Hit
TACTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT21521.177371703687493No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16030.8770106138527192No Hit
TACTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA11200.6127585074953497No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10410.5695371484845169No Hit
TACTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA9810.5367107998686946No Hit
TACTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC9260.5066199803041909No Hit
TACTTCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC9070.49622496990918047No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAGCAGT8400.4595688806215122No Hit
TACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA8060.44096728307254623No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7570.41415909836962467No Hit
TACTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT7260.3971988182514498No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5860.32060400481453116No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.2943429259218733No Hit
TACTTCGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4770.2609694714957873No Hit
TACTTCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4590.2511215669110406No Hit
TACTTCGGGGTGCTTACTTCGGGCAGTGGTAGCTTACTTCGGGCTTTTCCTGTGGG4440.24291497975708504No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4010.2193894299157457No Hit
TACTTCGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3730.20407046722836197No Hit
TACTTCGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3660.20024072655651604No Hit
TACTTCGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3610.19750519750519752No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3540.19367545683335155No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.1860159754896597No Hit
TACTTCGGGGCTTTTCCTGTAGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3370.1843746580588686No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA3250.17780938833570412No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG3230.17671517671517672Illumina Single End Adapter 2 (95% over 23bp)
TACTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.176168070904913No Hit
TACTTCGGGGCCTGTGAGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC3140.17179122442280337No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAGCAGG3110.17014990699201227No Hit
TACTTCGGGGCTTACTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCT3040.16632016632016633No Hit
TACTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.16084910821752926No Hit
TACTTCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2940.16084910821752926No Hit
TACTTCGGGCTTACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC2720.14881278039172777No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2710.14826567458146406No Hit
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.1422475106685633No Hit
TACTTCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG2520.13787066418645366Illumina Single End Adapter 2 (96% over 30bp)
TACTTCGGGAGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2520.13787066418645366No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.13568224094539885No Hit
TACTTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2460.1345880293248714No Hit
TACTTCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC2330.12747565379144327No Hit
TACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC2200.12036327825801509No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2040.11160958529379582No Hit
TACTTCGGGAGGCCAATCTTCTCTGAAGAATTCCTTCTTAAGGCTCCTTCCCAGTG1960.10723273881168618No Hit
TACTTCGGGGTGCTTACTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGA1960.10723273881168618No Hit
TACTTCGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG1890.10340299813984026No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT1830.10012036327825802Illumina Single End Adapter 2 (100% over 29bp)
TACTTCGGGAACCATTGTACTTACCATCACAGTAATATACCAATGTCTCTTGGACC1830.10012036327825802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAT150.00823238850.28528249
CAATTAT150.00823238850.28528244
TTATAGA150.00823238850.28528246
TGATTCG150.00823238850.28528248
GATATGG301.0028234E-650.28528250
TTTATAG150.00823238850.28528245
GATTCGG150.00823238850.28528249
TACCCAA204.058939E-450.2852844
GGCCGCT402.5302143E-950.2852849
AAGACCT204.058939E-450.2852849
CGTCGGG252.0148922E-550.271443
ACCTTCG355.0369636E-850.271442
TGATTTC150.008241381550.27139742
ACAATTA150.008241381550.27139743
AATTCGG301.0047352E-650.27139742
ACGTCGG150.008241381550.27139742
CACAATT150.008241381550.27139742
CGCCCTT150.008241381550.27139743
TCACAAT150.0082593950.24365641
AAGCTCT150.0082593950.24365641
Produced by FastQC (version 0.11.5)